##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551191_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	993396
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.31474658645696	33.0	31.0	34.0	30.0	34.0
2	32.41269141409871	34.0	31.0	34.0	30.0	34.0
3	32.444268952160066	34.0	31.0	34.0	30.0	34.0
4	35.94284655867348	37.0	35.0	37.0	35.0	37.0
5	35.98846079509078	37.0	35.0	37.0	35.0	37.0
6	36.0168049800885	37.0	35.0	37.0	35.0	37.0
7	36.16400408296389	37.0	36.0	37.0	35.0	37.0
8	35.81175080229838	37.0	35.0	37.0	35.0	37.0
9	37.70009341692538	39.0	38.0	39.0	35.0	39.0
10	37.580269097117366	39.0	37.0	39.0	35.0	39.0
11	37.50167304881437	39.0	37.0	39.0	35.0	39.0
12	37.406189475294845	39.0	37.0	39.0	35.0	39.0
13	37.366394670403345	39.0	37.0	39.0	35.0	39.0
14	38.61686578162183	40.0	38.0	41.0	35.0	41.0
15	38.6717311122654	40.0	38.0	41.0	35.0	41.0
16	38.72927311968238	40.0	38.0	41.0	35.0	41.0
17	38.725229415057036	40.0	38.0	41.0	35.0	41.0
18	38.71370631651426	40.0	38.0	41.0	35.0	41.0
19	38.714351577819926	40.0	38.0	41.0	35.0	41.0
20	38.64757156259941	40.0	38.0	41.0	35.0	41.0
21	38.595906365638676	40.0	38.0	41.0	35.0	41.0
22	38.57770617155696	40.0	38.0	41.0	35.0	41.0
23	38.54721983982218	40.0	38.0	41.0	35.0	41.0
24	38.48627536249391	40.0	38.0	41.0	34.0	41.0
25	38.408238003776944	40.0	37.0	41.0	34.0	41.0
26	38.403758420609705	40.0	37.0	41.0	34.0	41.0
27	38.36184260858711	40.0	37.0	41.0	34.0	41.0
28	38.31295575983797	40.0	37.0	41.0	34.0	41.0
29	38.31184341390543	40.0	37.0	41.0	34.0	41.0
30	38.20242078687653	40.0	37.0	41.0	34.0	41.0
31	38.16324507044522	40.0	37.0	41.0	34.0	41.0
32	38.08825785487358	40.0	37.0	41.0	34.0	41.0
33	38.03905391203508	40.0	37.0	41.0	34.0	41.0
34	37.96307917487085	40.0	37.0	41.0	34.0	41.0
35	37.890505900970005	40.0	37.0	41.0	34.0	41.0
36	37.85375117274481	40.0	37.0	41.0	34.0	41.0
37	37.79600078921196	40.0	36.0	41.0	33.0	41.0
38	37.7199797462442	40.0	36.0	41.0	33.0	41.0
39	37.65041634957258	40.0	36.0	41.0	33.0	41.0
40	37.554804931769404	40.0	36.0	41.0	33.0	41.0
41	37.510782205686354	40.0	36.0	41.0	33.0	41.0
42	37.50470305900165	40.0	36.0	41.0	33.0	41.0
43	37.45709062649739	40.0	35.0	41.0	33.0	41.0
44	37.39237725942122	40.0	35.0	41.0	33.0	41.0
45	37.33539092164656	40.0	35.0	41.0	33.0	41.0
46	37.20322610519873	39.0	35.0	41.0	33.0	41.0
47	37.15253031016835	39.0	35.0	41.0	33.0	41.0
48	37.125614558544626	39.0	35.0	41.0	33.0	41.0
49	37.03055377714426	39.0	35.0	41.0	32.0	41.0
50	36.974079823152096	39.0	35.0	41.0	32.0	41.0
51	36.195405457642266	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	18.0
10	13.0
11	13.0
12	18.0
13	29.0
14	27.0
15	42.0
16	80.0
17	97.0
18	177.0
19	284.0
20	493.0
21	819.0
22	1285.0
23	1880.0
24	2743.0
25	3848.0
26	5346.0
27	6817.0
28	7697.0
29	9090.0
30	10782.0
31	13978.0
32	18245.0
33	25580.0
34	49573.0
35	75216.0
36	61631.0
37	94762.0
38	176355.0
39	425565.0
40	888.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.219823715819274	21.441600328569876	23.039955868555946	12.29862008705491
2	32.47476333707806	25.81870673930638	23.868427092518996	17.83810283109656
3	32.81490966341721	24.631667532383865	25.333804444551824	17.21961835964711
4	29.833520569843248	25.175056070288182	25.362997233731566	19.628426126137008
5	26.011479812682957	30.47374863599209	23.169712783220387	20.34505876810456
6	25.780152124631062	34.70489110082988	23.71591993525241	15.799036839286648
7	78.21724669718823	6.5357621733930875	9.723010763079376	5.523980366339305
8	77.90991709247874	6.909933198845174	9.032752296163867	6.147397412512231
9	71.66366685591647	8.875312564173804	11.6127908709115	7.848229708998224
10	39.68125500807332	28.100777534840084	17.97037636551788	14.24759109156872
11	28.162283721698095	25.307933593451153	26.419071548506334	20.11071113634442
12	26.622817084022888	22.69155502941425	29.427338141083716	21.258289745479146
13	24.18793713685177	24.471509851056375	31.24101566746796	20.099537344623897
14	19.983571506227126	28.043599933963897	29.177790125992054	22.795038433816927
15	19.94632553382538	26.70858348533717	32.77021449653512	20.574876484302333
16	22.192559664021196	26.7732102806937	29.63148633576137	21.402743719523734
17	21.53793653286303	27.732545732014223	27.2004316506207	23.52908608450205
18	22.430631893021513	26.76183515939263	28.937301942025133	21.870231005560722
19	23.196992941384906	27.63641085730162	27.40206322554148	21.764532975772
20	24.340343629328082	27.433369975316996	27.924010163117224	20.3022762322377
21	24.133880144474105	27.310961590342625	28.762749195688325	19.792409069494944
22	22.34355684943366	25.073988620852106	27.985919009136335	24.5965355205779
23	21.725676366725857	26.382026905685148	29.083869876665496	22.8084268509235
24	23.002307236993104	25.277532826788107	29.05719370724263	22.66296622897616
25	21.88955864529352	27.778750870750436	26.770592996146554	23.561097487809494
26	20.5963180846309	27.775428932671364	27.41998155821042	24.208271424487315
27	22.033307965806184	27.160266399300987	28.11607858296188	22.69034705193095
28	19.853915256352956	27.246435459776364	29.664806381342384	23.234842902528296
29	22.25185122549316	25.398028580747255	27.562321571659236	24.78779862210035
30	21.369625003523268	25.730826377396326	28.37961900390177	24.51992961517864
31	22.448952884851558	27.223987211545044	26.255189269938672	24.071870633664723
32	22.636894048294938	27.352032824774813	26.661069704327378	23.350003422602867
33	21.822515894970383	26.376993666171394	26.989941574155722	24.810548864702493
34	20.74489931507677	26.37085311396462	28.638126185327906	24.246121385630705
35	21.123096932139852	26.432057306451807	26.945850396015285	25.498995365393057
36	21.048806316916917	27.308847629746847	26.736266302662788	24.90607975067345
37	21.925294645841134	27.433168645736444	27.99568349379301	22.645853214629412
38	21.29382441644621	27.765664448014686	27.26576309950916	23.674748036029943
39	22.71923784673987	25.510471151484403	27.977664496333787	23.792626505441937
40	21.6494731204877	25.986716274275313	29.169032289238128	23.194778315998857
41	19.32703574405373	27.382735585808682	26.980881743030977	26.309346927106613
42	20.531389294903544	25.907191089958083	27.96759801730629	25.593821597832083
43	22.216417219316366	24.058683546138703	28.058699652505144	25.666199582039788
44	21.632561435721502	25.08979299292528	27.647886643392965	25.62975892796025
45	21.670713391235722	24.78739596293925	27.432363327414244	26.109527318410784
46	22.424592005605014	26.112849256489863	27.36089132631901	24.101667411586114
47	20.58111770129938	25.81468014769538	29.51662781005762	24.08757434094762
48	20.376365517880078	24.67515472178265	29.208996210977293	25.73948354935997
49	21.136485349246424	23.78296268557554	30.344394380488747	24.736157584689288
50	20.150473728503034	23.832791756761655	30.02176372765745	25.994970787077865
51	20.24207868765326	23.6358914269838	28.0431972748028	28.078832610560138
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	468.0
1	997.5
2	1527.0
3	2980.0
4	4433.0
5	3116.0
6	1799.0
7	1689.5
8	1580.0
9	1582.5
10	1585.0
11	1560.0
12	1535.0
13	1572.5
14	1610.0
15	1653.0
16	1696.0
17	1691.5
18	1687.0
19	1779.0
20	1871.0
21	2723.0
22	3575.0
23	3684.0
24	3793.0
25	5096.5
26	7769.5
27	9139.0
28	10235.0
29	11331.0
30	14716.0
31	18101.0
32	19640.0
33	21179.0
34	24049.0
35	26919.0
36	28711.5
37	30504.0
38	33572.0
39	36640.0
40	41480.5
41	46321.0
42	50113.0
43	53905.0
44	60202.0
45	66499.0
46	78861.5
47	91224.0
48	96805.0
49	102386.0
50	98701.0
51	95016.0
52	84027.0
53	73038.0
54	64726.0
55	56414.0
56	51722.0
57	47030.0
58	43754.0
59	40478.0
60	38266.5
61	36055.0
62	33226.0
63	30397.0
64	26970.0
65	23543.0
66	20072.5
67	16602.0
68	13619.0
69	10636.0
70	8704.5
71	6773.0
72	5609.0
73	4445.0
74	3431.0
75	1932.0
76	1447.0
77	1126.5
78	806.0
79	568.5
80	331.0
81	233.0
82	135.0
83	109.5
84	84.0
85	49.0
86	14.0
87	19.0
88	24.0
89	12.5
90	1.0
91	1.5
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	993396.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.694416798383035
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.02297420648604	18.95916875728746
2	11.30422612538671	6.035194475484866
3	4.021827765360851	3.2208103997955604
4	2.0746582853899462	2.2152717193767173
5	1.2541454976642974	1.6739341320233136
6	0.8544862995278417	1.3686008056862506
7	0.6583515585082361	1.2302017631878734
8	0.5255077759077829	1.1222498880698901
9	0.46096392715710455	1.1074646880496102
>10	7.171947089867217	47.12545864948924
>50	0.5683046128878354	9.138577963408686
>100	0.07304587716912858	3.663496062544998
>500	0.005354148064752857	1.0571409148015427
>1k	0.004206830622305816	2.082429780794
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	3663	0.36873512677723685	Illumina PCR Primer Index 2 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2443	0.24592408264176624	No Hit
CCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	2314	0.2329383246962943	Illumina PCR Primer Index 2 (95% over 24bp)
GAATCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTC	2145	0.21592597513982337	Illumina PCR Primer Index 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCT	1872	0.18844448739475497	Illumina PCR Primer Index 2 (96% over 25bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCG	1752	0.17636471256175784	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGC	1523	0.15331247558878836	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1247	0.125528993472895	No Hit
ATCCGCTAGTTCTGGCCCCTGGCCTCTGGCATAATATCTTCACAATGGTGC	1246	0.12542832868262002	No Hit
TCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	1168	0.1175764750411719	Illumina PCR Primer Index 2 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCC	1025	0.10318141003185033	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.039887416498556E-4	0.0	0.0	0.19428304523070355	0.0
2	6.039887416498556E-4	0.0	0.0	1.0310087819963036	0.0
3	6.039887416498556E-4	0.0	0.0	1.4022605285304148	0.0
4	6.039887416498556E-4	0.0	0.0	1.9270260802338643	0.0
5	6.039887416498556E-4	0.0	0.0	3.544105271211078	0.0
6	6.039887416498556E-4	0.0	0.0	4.530016227164192	0.0
7	6.039887416498556E-4	0.0	0.0	5.3901968600638615	0.0
8	6.039887416498556E-4	0.0	0.0	6.640554220069338	0.0
9	6.039887416498556E-4	0.0	0.0	7.1408582277359685	0.0
10	6.039887416498556E-4	0.0	0.0	8.302328577928641	0.0
11	6.039887416498556E-4	0.0	0.0	9.722910098289102	0.0
12	6.039887416498556E-4	0.0	0.0	10.989575154319123	0.0
13	7.046535319248316E-4	0.0	0.0	11.447700614860539	0.0
14	7.046535319248316E-4	0.0	0.0	11.63664842620667	0.0
15	7.046535319248316E-4	0.0	0.0	12.021892578589002	0.0
16	7.046535319248316E-4	0.0	0.0	12.712251710294787	0.0
17	7.046535319248316E-4	0.0	0.0	13.543843542756363	0.0
18	7.046535319248316E-4	0.0	0.0	14.442679455121624	0.0
19	7.046535319248316E-4	0.0	0.0	15.028850528892809	0.0
20	7.046535319248316E-4	0.0	0.0	15.577775630262252	0.0
21	7.046535319248316E-4	0.0	0.0	16.242867899609017	0.0
22	7.046535319248316E-4	0.0	0.0	16.94671611321165	0.0
23	7.046535319248316E-4	0.0	0.0	17.648047707057408	0.0
24	7.046535319248316E-4	0.0	0.0	18.209958566372322	0.0
25	7.046535319248316E-4	0.0	0.0	18.718819081212327	0.0
26	8.053183221998075E-4	0.0	0.0	19.175635899480167	0.0
27	8.053183221998075E-4	0.0	0.0	19.652585675803003	0.0
28	8.053183221998075E-4	0.0	0.0	20.13094475918969	0.0
29	8.053183221998075E-4	0.0	0.0	20.607089217190325	0.0
30	8.053183221998075E-4	0.0	0.0	21.166886115909467	0.0
31	9.059831124747835E-4	0.0	0.0	21.67775992655497	0.0
32	9.059831124747835E-4	0.0	0.0	22.160950919874853	0.0
33	9.059831124747835E-4	0.0	0.0	22.644947231516937	0.0
34	9.059831124747835E-4	0.0	0.0	23.10236803852643	0.0
35	9.059831124747835E-4	0.0	0.0	23.618174423895404	0.0
36	9.059831124747835E-4	0.0	0.0	24.110827907501136	0.0
37	9.059831124747835E-4	0.0	0.0	24.57901984707005	0.0
38	9.059831124747835E-4	0.0	0.0	25.049426412025014	0.0
39	9.059831124747835E-4	0.0	0.0	25.502015309101306	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTCACG	25	3.890421E-5	45.000004	1
GTACGAG	50	2.1827873E-11	45.000004	1
GTCGATG	35	1.2117016E-7	45.0	1
CCGTACA	20	7.032823E-4	45.0	39
GTATACG	20	7.032823E-4	45.0	1
CCGTTCG	20	7.032823E-4	45.0	10
AAATGCG	35	1.2117016E-7	45.0	1
GTCAACG	30	2.1652031E-6	44.999996	1
CGAATAT	235	0.0	43.085106	14
TACGAAT	240	0.0	42.187496	12
GCTACGA	245	0.0	41.32653	10
CGAAAGG	55	6.002665E-11	40.909092	2
CATACGA	160	0.0	40.78125	18
CGTAAGG	105	0.0	40.714283	2
CTACGAA	255	0.0	40.588234	11
CGTATGG	100	0.0	40.500004	2
CCTATGC	235	0.0	40.212765	35
GGTACGT	45	1.927765E-8	40.000004	8
GGCCGAT	140	0.0	38.571426	8
TACGGGA	415	0.0	38.493977	4
>>END_MODULE