FastQCFastQC Report
Sat 18 Jun 2016
SRR3551189_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551189_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1312499
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66350.5055241946851007No Hit
GCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC30500.23238112943324146TruSeq Adapter, Index 19 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTC28900.22019064395477636No Hit
CCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC21920.16700965105497223TruSeq Adapter, Index 19 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCT21870.1666286983837702TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCG19100.14552392039917744No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT17660.13455248346855883No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGC16960.12921914607173032No Hit
ATCCGCTAGTTCTGGCCCCTGGCCTCTGGCATAATATCTTCACAATGGTGC13790.10506674671752131No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGAG207.0336525E-445.00000417
CGTATGG1350.043.3333322
GTTAACG650.041.5384641
GCTACGA3350.040.29850810
CGTTTTT32700.040.1146771
TATAGCG451.9286745E-840.01
CGCGACT356.2489653E-638.5714332
TTAGTCG356.2489653E-638.571431
TATACGG356.2489653E-638.571432
TTTAGCG659.094947E-1238.0769271
GCGTTAG659.094947E-1238.0769271
CGAATAT3500.037.9285714
CTACCGT301.1400099E-437.50000412
CGCACGG1200.037.5000042
CGGTAAT301.1400099E-437.5000046
AGCTACG3600.036.8759
TCGTAAG1100.036.818181
TACAGCG1100.036.818181
CGTAAGG2550.036.176472
ATTGCGG2250.036.02