##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551189_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1312499
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.386774389923346	33.0	31.0	34.0	30.0	34.0
2	32.492810280236405	34.0	31.0	34.0	31.0	34.0
3	32.51137867533613	34.0	31.0	34.0	31.0	34.0
4	36.003389716868355	37.0	35.0	37.0	35.0	37.0
5	36.04062098333027	37.0	35.0	37.0	35.0	37.0
6	36.066580622156664	37.0	36.0	37.0	35.0	37.0
7	36.20545768034871	37.0	37.0	37.0	35.0	37.0
8	35.8855473413694	37.0	36.0	37.0	35.0	37.0
9	37.76373924856324	39.0	38.0	39.0	35.0	39.0
10	37.65440964145496	39.0	37.0	39.0	35.0	39.0
11	37.590709021492586	39.0	37.0	39.0	35.0	39.0
12	37.51541524984019	39.0	37.0	39.0	35.0	39.0
13	37.496335616255706	39.0	37.0	39.0	35.0	39.0
14	38.810258903054404	40.0	38.0	41.0	35.0	41.0
15	38.87816524050685	40.0	38.0	41.0	35.0	41.0
16	38.92178584517017	40.0	38.0	41.0	35.0	41.0
17	38.91545745939616	40.0	38.0	41.0	35.0	41.0
18	38.89048753560955	40.0	38.0	41.0	35.0	41.0
19	38.85872827331678	40.0	38.0	41.0	35.0	41.0
20	38.755809337759494	40.0	38.0	41.0	35.0	41.0
21	38.72031292976223	40.0	38.0	41.0	35.0	41.0
22	38.70470072739103	40.0	38.0	41.0	35.0	41.0
23	38.6624705999776	40.0	38.0	41.0	35.0	41.0
24	38.628626002762665	40.0	38.0	41.0	35.0	41.0
25	38.55087584828636	40.0	38.0	41.0	35.0	41.0
26	38.52380687528143	40.0	38.0	41.0	35.0	41.0
27	38.463856353414364	40.0	38.0	41.0	35.0	41.0
28	38.385965246449715	40.0	38.0	41.0	35.0	41.0
29	38.35492598470551	40.0	37.0	41.0	34.0	41.0
30	38.265466107021794	40.0	37.0	41.0	34.0	41.0
31	38.19476967220547	40.0	37.0	41.0	34.0	41.0
32	38.07412272314112	40.0	37.0	41.0	34.0	41.0
33	38.00987124561619	40.0	37.0	41.0	34.0	41.0
34	37.93050508990864	40.0	37.0	41.0	34.0	41.0
35	37.83546958892921	40.0	37.0	41.0	34.0	41.0
36	37.801854325222344	40.0	37.0	41.0	33.0	41.0
37	37.73124322380436	40.0	37.0	41.0	33.0	41.0
38	37.65906640690774	40.0	37.0	41.0	33.0	41.0
39	37.612414180886994	40.0	36.0	41.0	33.0	41.0
40	37.536805742328184	40.0	36.0	41.0	33.0	41.0
41	37.469873881808674	40.0	36.0	41.0	33.0	41.0
42	37.4485915798793	40.0	36.0	41.0	33.0	41.0
43	37.378705050441944	40.0	36.0	41.0	33.0	41.0
44	37.3302920611749	40.0	35.0	41.0	33.0	41.0
45	37.259655816880624	40.0	35.0	41.0	33.0	41.0
46	37.143375347333595	40.0	35.0	41.0	32.0	41.0
47	37.10136083913207	40.0	35.0	41.0	32.0	41.0
48	37.06268804776232	39.0	35.0	41.0	32.0	41.0
49	36.95693863385801	39.0	35.0	41.0	32.0	41.0
50	36.90757021529159	39.0	35.0	41.0	32.0	41.0
51	36.188286619646945	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	10.0
10	15.0
11	19.0
12	10.0
13	34.0
14	32.0
15	65.0
16	83.0
17	113.0
18	239.0
19	456.0
20	852.0
21	1305.0
22	2012.0
23	2886.0
24	3991.0
25	6048.0
26	8565.0
27	10368.0
28	11225.0
29	12388.0
30	14269.0
31	17462.0
32	22202.0
33	30801.0
34	57682.0
35	89813.0
36	78343.0
37	119494.0
38	228888.0
39	591577.0
40	1245.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.541670507939436	20.95140643916681	22.896855540461363	13.610067512432392
2	31.903871926759564	25.30500975619791	24.089618353994936	18.701499963047592
3	31.689167001270096	24.187904143165063	26.308515282678314	17.81441357288653
4	28.492745518282298	25.889695915958793	26.388058200425295	19.229500365333614
5	25.85160064883859	29.529241546088798	24.928247564379095	19.690910240693515
6	26.012286485551606	34.07918786985743	24.082913586981782	15.825612057609186
7	78.3892406775167	6.4429763375057805	10.104160079360062	5.063622905617452
8	79.49263199438629	6.439623953999203	8.959625874000666	5.10811817761385
9	73.10992236946467	8.538368410185456	11.318103861412466	7.033605358937417
10	38.016409917264696	30.20139443915767	17.936166046602704	13.846029596974931
11	27.71407825834534	25.439867001803428	27.73419255938481	19.111862180466424
12	26.243524756971244	23.343865404849833	30.23895637253819	20.173653465640736
13	24.0005516194679	24.27727563982906	31.23309046330702	20.48908227739602
14	19.405957642634394	28.71339330544252	29.692136908294785	22.1885121436283
15	19.4444338624258	27.250535048026702	33.12452047582512	20.180510613722372
16	21.541578317393004	27.063487286466504	30.95560453760346	20.439329858537036
17	21.451749677523562	27.183715949497866	28.64992659042026	22.71460778255831
18	22.02561678142231	26.769087062161574	29.621660664122412	21.58363549229371
19	23.15986526465925	27.62074485390084	28.181050042704797	21.038339838735116
20	24.79194269862301	26.963144352871886	28.961317303860802	19.2835956446443
21	24.56984729131222	27.127106382557244	28.898764875249427	19.404281450881104
22	22.766722107978747	25.22836207875206	28.64825039866697	23.356665414602222
23	21.75171181082805	26.856096652264117	29.39507001529144	21.997121521616396
24	21.930835756827243	25.76344820072244	29.65693688147572	22.6487791609746
25	22.0503025145162	28.342193022623256	27.50623048093751	22.101273981923033
26	21.0499969904739	28.792783842121022	27.48162093837786	22.675598229027223
27	22.042988223229123	28.274535828217772	28.29571679673661	21.3867591518165
28	20.13753915241078	28.9788411267361	28.97967922261274	21.903940498240377
29	20.85395874587333	27.892440299002132	28.467450260914482	22.786150694210054
30	21.00900648305256	27.70135443912719	28.2532786691647	23.036360408655547
31	21.22584474350076	29.097774550685372	26.002153144497637	23.67422756131624
32	21.46607349796076	29.835603684269472	26.40200106819129	22.29632174957848
33	20.728015792773938	28.522536017170296	26.67346794168986	24.0759802483659
34	19.920472320359863	28.193011956580538	27.51590667878604	24.370609044273557
35	20.277120211139206	26.98310627284288	26.88703000916572	25.852743506852192
36	20.31666309840998	29.23324132056482	26.130534194692718	24.319561386332484
37	20.68169194795577	29.019603062554715	26.922306226519026	23.376398762970485
38	19.834224635599725	28.437354999889525	27.2493921900131	24.479028174497657
39	21.329463870067713	26.28992479232365	27.688325857772085	24.692285479836556
40	21.17395898968304	25.688857667701082	29.446270054300992	23.690913288314885
41	18.73692856146938	26.675982229319793	28.040173744894282	26.546915464316545
42	20.270948777865737	26.894725253124	27.25861124465619	25.575714724354075
43	20.93647309445569	26.81952519582872	27.913240314849762	24.330761394865824
44	21.22439712335019	27.09617302565564	27.25487790847841	24.424551942515766
45	20.82774920209463	26.309505759623438	27.09792540794317	25.764819630338764
46	20.73053008040387	27.725125885810197	26.863563324619676	24.680780709166257
47	20.460358445987385	26.14767706489681	29.132136481627796	24.259828007488004
48	20.1743391804489	25.5357908844121	29.006879243336563	25.282990691802432
49	20.24999638094962	25.835524446113862	28.917507746672573	24.996971426263944
50	19.19338605210366	25.326266915250983	29.698460722636742	25.781886310008616
51	19.381500481143224	24.608399701637868	27.769011633532674	28.241088183686237
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	578.0
1	1324.0
2	2070.0
3	6557.0
4	11044.0
5	7358.5
6	3673.0
7	3561.5
8	3450.0
9	3374.5
10	3299.0
11	3283.0
12	3267.0
13	3206.5
14	3146.0
15	3130.5
16	3115.0
17	3213.5
18	3312.0
19	3430.5
20	3549.0
21	3813.0
22	4077.0
23	5303.5
24	6530.0
25	7897.5
26	10994.0
27	12723.0
28	14352.5
29	15982.0
30	19769.5
31	23557.0
32	26204.5
33	28852.0
34	33758.5
35	38665.0
36	41468.5
37	44272.0
38	48911.5
39	53551.0
40	58732.5
41	63914.0
42	70759.5
43	77605.0
44	84180.0
45	90755.0
46	103716.5
47	116678.0
48	121255.5
49	125833.0
50	122388.0
51	118943.0
52	105977.5
53	93012.0
54	82537.5
55	72063.0
56	65669.0
57	59275.0
58	55513.5
59	51752.0
60	50337.5
61	48923.0
62	43910.5
63	38898.0
64	32745.0
65	26592.0
66	23082.0
67	19572.0
68	15664.0
69	11756.0
70	9719.5
71	7683.0
72	6213.5
73	4744.0
74	3949.5
75	2443.0
76	1731.0
77	1330.0
78	929.0
79	627.5
80	326.0
81	308.0
82	290.0
83	174.0
84	58.0
85	41.0
86	24.0
87	13.5
88	3.0
89	5.0
90	7.0
91	4.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1312499.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.77577156878278
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.85100894189742	18.520151934729167
2	10.5870227470315	5.457773598419823
3	3.794280080237819	2.934014898485785
4	1.987222968636636	2.0488882118326526
5	1.202155634861579	1.549324451715855
6	0.8571422072961788	1.3256101042337136
7	0.629596104249317	1.135982775460816
8	0.5290141731870164	1.0908598787773622
9	0.4511855627073675	1.046669039953004
>10	7.285780096386414	46.61116312669281
>50	0.7453098455538049	11.745525987126548
>100	0.07069577073763884	3.3145428909915906
>500	0.005392050309742678	0.957215463840584
>1k	0.0038942585570363787	1.7499662234801157
>5k	2.995583505412599E-4	0.5123114142600851
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6635	0.5055241946851007	No Hit
GCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC	3050	0.23238112943324146	TruSeq Adapter, Index 19 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTC	2890	0.22019064395477636	No Hit
CCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC	2192	0.16700965105497223	TruSeq Adapter, Index 19 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCT	2187	0.1666286983837702	TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCG	1910	0.14552392039917744	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1766	0.13455248346855883	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGC	1696	0.12921914607173032	No Hit
ATCCGCTAGTTCTGGCCCCTGGCCTCTGGCATAATATCTTCACAATGGTGC	1379	0.10506674671752131	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.619053424040704E-5	0.0	0.0	0.1731810843284452	0.0
2	7.619053424040704E-5	0.0	0.0	0.708495777901545	0.0
3	7.619053424040704E-5	0.0	0.0	1.0155436308903854	0.0
4	7.619053424040704E-5	0.0	0.0	1.4510487246085522	0.0
5	7.619053424040704E-5	0.0	0.0	2.664535363455515	0.0
6	7.619053424040704E-5	0.0	0.0	3.5378312669190604	0.0
7	7.619053424040704E-5	0.0	0.0	4.229336555684995	0.0
8	7.619053424040704E-5	0.0	0.0	5.230556366138184	0.0
9	7.619053424040704E-5	0.0	0.0	5.705604347127122	0.0
10	7.619053424040704E-5	0.0	0.0	6.759776578877394	0.0
11	7.619053424040704E-5	0.0	0.0	7.937225095028644	0.0
12	7.619053424040704E-5	0.0	0.0	9.067816432622044	0.0
13	1.5238106848081408E-4	0.0	0.0	9.518559633188293	0.0
14	2.285716027212211E-4	0.0	0.0	9.735093131499529	0.0
15	2.285716027212211E-4	0.0	0.0	10.082521967635785	0.0
16	2.285716027212211E-4	0.0	0.0	10.660808122520475	0.0
17	2.285716027212211E-4	0.0	0.0	11.347284836026542	0.0
18	2.285716027212211E-4	0.0	0.0	12.142866394564873	0.0
19	2.285716027212211E-4	0.0	0.0	12.655857261605533	0.0
20	2.285716027212211E-4	0.0	0.0	13.154448117674756	0.0
21	2.285716027212211E-4	0.0	0.0	13.735781893929062	0.0
22	2.285716027212211E-4	0.0	0.0	14.360239512563439	0.0
23	2.285716027212211E-4	0.0	0.0	14.957497110473989	0.0
24	2.285716027212211E-4	0.0	0.0	15.451287962886067	0.0
25	2.285716027212211E-4	0.0	0.0	15.898450208343016	0.0
26	2.285716027212211E-4	0.0	0.0	16.29829813203667	0.0
27	2.285716027212211E-4	0.0	0.0	16.735479417508127	0.0
28	2.285716027212211E-4	0.0	0.0	17.146070206529682	0.0
29	2.285716027212211E-4	0.0	0.0	17.565041954317678	0.0
30	3.0476213696162816E-4	0.0	0.0	18.07384234197512	0.0
31	3.809526712020352E-4	0.0	0.0	18.524661733075607	0.0
32	3.809526712020352E-4	0.0	0.0	18.978452555011472	0.0
33	3.809526712020352E-4	0.0	0.0	19.385157626786764	0.0
34	3.809526712020352E-4	0.0	0.0	19.807024614875896	0.0
35	3.809526712020352E-4	0.0	0.0	20.25494876567525	0.0
36	3.809526712020352E-4	0.0	0.0	20.68915862031133	0.0
37	3.809526712020352E-4	0.0	0.0	21.134035149741067	0.0
38	3.809526712020352E-4	0.0	0.0	21.556816424241084	0.0
39	4.571432054424422E-4	0.0	0.0	21.982340557973757	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCGAG	20	7.0336525E-4	45.000004	17
CGTATGG	135	0.0	43.333332	2
GTTAACG	65	0.0	41.538464	1
GCTACGA	335	0.0	40.298508	10
CGTTTTT	3270	0.0	40.114677	1
TATAGCG	45	1.9286745E-8	40.0	1
CGCGACT	35	6.2489653E-6	38.57143	32
TTAGTCG	35	6.2489653E-6	38.57143	1
TATACGG	35	6.2489653E-6	38.57143	2
TTTAGCG	65	9.094947E-12	38.076927	1
GCGTTAG	65	9.094947E-12	38.076927	1
CGAATAT	350	0.0	37.92857	14
CTACCGT	30	1.1400099E-4	37.500004	12
CGCACGG	120	0.0	37.500004	2
CGGTAAT	30	1.1400099E-4	37.500004	6
AGCTACG	360	0.0	36.875	9
TCGTAAG	110	0.0	36.81818	1
TACAGCG	110	0.0	36.81818	1
CGTAAGG	255	0.0	36.17647	2
ATTGCGG	225	0.0	36.0	2
>>END_MODULE