FastQCFastQC Report
Sat 18 Jun 2016
SRR3551187_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551187_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences872475
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21160.24252843921029255No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCG20760.23794378062408664No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGC18250.20917504799564454No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTC18010.20642425284392105No Hit
GCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC14580.16711080546720536No Hit
ATCCGCTAGTTCTGGCCCCTGGCCTCTGGCATAATATCTTCACAATGGTGC10310.1181695750594573No Hit
GCTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAACT10200.11690879394825066No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGT253.8900307E-545.019
CCGTCGA207.032352E-445.041
TCGACGG253.8900307E-545.02
GATTACG351.2114833E-745.01
TCGGTAC207.032352E-445.015
CGAATAT1150.044.99999614
CGGTCTA700.041.78571331
ATTACGG650.041.5384642
ATCGCAT501.0804797E-940.510
TACGAAT1300.039.80769312
ATTTACG356.2465606E-638.5714261
TCGATAG356.2465606E-638.5714261
GTCGAAT301.1396961E-437.50000443
AGCGGTA301.1396961E-437.50000418
ACGACTT301.1396961E-437.50000413
CGTAAGG1300.036.3461572
TCGGCGT1800.036.254
TAGGGCG1800.036.255
TATCACG250.002106831236.038
TCGATCT250.002106831236.035
Produced by FastQC (version 0.11.5)