FastQCFastQC Report
Sat 18 Jun 2016
SRR3551184_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551184_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences726902
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCG74881.0301251062729226No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGC72120.9921557513942734No Hit
GAATCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTC63930.8794858178956724No Hit
GCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC27190.37405317360524526No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGGTGGCTT21570.2967387625842273No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20930.28793427449642456No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCT15070.20731818044248057No Hit
CCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC11840.16288302962435103No Hit
GAACTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCT11650.16026919722328456No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTA11440.1573802245694743No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT10160.13977124839386876No Hit
CTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGCT8910.12257498259737902No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTC8410.1156964762787831No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATGCG351.2111013E-745.0000041
CGCATCG253.8893864E-545.021
ATCGTGT207.0315733E-445.042
CGTAAGC253.8893864E-545.043
TAGGCCG253.8893864E-545.029
GAACCCG207.0315733E-445.045
GACGCAC253.8893864E-545.033
CGCCCAC207.0315733E-445.034
AATCGTC253.8893864E-545.040
AGGTCGA253.8893864E-545.011
ATCCCGT302.1643973E-644.99999628
CGAAAGG800.042.18752
GCTACGG1400.041.7857172
CGGGTAT650.041.538466
TACGAGG650.041.538462
CGTAAGG1200.041.2499962
TACGGCT8500.040.7647067
CCGATGA9100.040.5494518
ACGGCTG8600.040.29078
CTACGGG2150.039.7674453