##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551184_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	726902
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.34562155558796	33.0	31.0	34.0	30.0	34.0
2	32.4500276515954	34.0	31.0	34.0	30.0	34.0
3	32.480334350435136	34.0	31.0	34.0	30.0	34.0
4	35.97986248490168	37.0	35.0	37.0	35.0	37.0
5	36.00918555733785	37.0	35.0	37.0	35.0	37.0
6	36.03624560119521	37.0	35.0	37.0	35.0	37.0
7	36.16589443969063	37.0	36.0	37.0	35.0	37.0
8	35.713273591213124	37.0	35.0	37.0	35.0	37.0
9	37.676109021573744	39.0	38.0	39.0	35.0	39.0
10	37.58980302709306	39.0	37.0	39.0	35.0	39.0
11	37.507207298920626	39.0	37.0	39.0	35.0	39.0
12	37.343187940052445	39.0	37.0	39.0	35.0	39.0
13	37.25245081180132	39.0	37.0	39.0	34.0	39.0
14	38.529944889407375	40.0	38.0	41.0	34.0	41.0
15	38.55682334069792	40.0	38.0	41.0	34.0	41.0
16	38.68214835012147	40.0	38.0	41.0	35.0	41.0
17	38.626948336914744	40.0	38.0	41.0	35.0	41.0
18	38.627548142665724	40.0	38.0	41.0	35.0	41.0
19	38.630127582535195	40.0	38.0	41.0	35.0	41.0
20	38.44127544015562	40.0	37.0	41.0	34.0	41.0
21	38.4777989330061	40.0	37.0	41.0	34.0	41.0
22	38.41491012543644	40.0	37.0	41.0	34.0	41.0
23	38.309025150570506	40.0	37.0	41.0	34.0	41.0
24	38.29430239564618	40.0	37.0	41.0	34.0	41.0
25	38.31747195632974	40.0	37.0	41.0	34.0	41.0
26	38.25289241190697	40.0	37.0	41.0	34.0	41.0
27	38.13035732464624	40.0	37.0	41.0	34.0	41.0
28	38.08645594591843	40.0	37.0	41.0	34.0	41.0
29	38.048369656432364	40.0	36.0	41.0	34.0	41.0
30	37.983534231574545	40.0	36.0	41.0	34.0	41.0
31	37.94663654798033	40.0	36.0	41.0	34.0	41.0
32	37.72978475778028	40.0	36.0	41.0	33.0	41.0
33	37.687037592412736	40.0	36.0	41.0	33.0	41.0
34	37.568120599475584	40.0	36.0	41.0	33.0	41.0
35	37.47369659183769	40.0	36.0	41.0	33.0	41.0
36	37.424842688560496	40.0	36.0	41.0	33.0	41.0
37	37.30648285463515	40.0	35.0	41.0	33.0	41.0
38	37.230275883131426	40.0	35.0	41.0	33.0	41.0
39	37.12711204536512	40.0	35.0	41.0	33.0	41.0
40	37.119130226633025	40.0	35.0	41.0	32.0	41.0
41	37.07104946746604	40.0	35.0	41.0	32.0	41.0
42	37.02819637310119	40.0	35.0	41.0	32.0	41.0
43	36.94684015176736	40.0	35.0	41.0	32.0	41.0
44	36.92595563088284	39.0	35.0	41.0	32.0	41.0
45	36.88704116923602	39.0	35.0	41.0	32.0	41.0
46	36.732935113674195	39.0	35.0	41.0	32.0	41.0
47	36.62897749627873	39.0	35.0	41.0	31.0	41.0
48	36.61682730271756	39.0	35.0	41.0	31.0	41.0
49	36.51548076632063	39.0	35.0	41.0	31.0	41.0
50	36.441202527988644	39.0	35.0	41.0	31.0	41.0
51	35.689672060332754	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	7.0
10	9.0
11	13.0
12	10.0
13	12.0
14	15.0
15	25.0
16	52.0
17	69.0
18	128.0
19	266.0
20	667.0
21	1410.0
22	2315.0
23	3103.0
24	3719.0
25	4485.0
26	5023.0
27	5676.0
28	5925.0
29	6651.0
30	7801.0
31	10090.0
32	13480.0
33	19116.0
34	38324.0
35	59382.0
36	45750.0
37	68656.0
38	126160.0
39	297918.0
40	645.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.44353709303317	19.313194901100836	22.391464048798877	11.851803957067116
2	31.009819755620427	29.6127951223136	23.289384263628385	16.08800085843759
3	30.592019281828915	28.161292718963495	24.432592013779022	16.814095985428573
4	27.860701992840852	24.14341960814525	29.631779799752923	18.364098599260974
5	28.890276818608285	28.706345559649034	23.11604590439977	19.287331717342916
6	24.46230716107536	36.78432581008169	23.31001978258417	15.443347246258782
7	76.29749264687675	6.008375269293522	13.140698471045614	4.553433612784117
8	76.5303988708244	10.21692057526324	8.339776200918418	4.912904352993939
9	71.60139881304495	7.616982756960361	9.645729410567036	11.135889019427653
10	43.251222310572814	25.058123378392132	17.41761612982218	14.273038181212874
11	32.56284891223301	24.31042974156076	23.87075561767611	19.255965728530118
12	27.339311213891282	22.29488982008579	27.82974871440717	22.536050251615762
13	26.08522194188488	23.555032177652556	30.59463311422998	19.765112766232587
14	20.622862504161496	29.362004781937596	27.779122907902305	22.23600980599861
15	19.214144410113054	25.04491664626043	34.214928559833375	21.52601038379314
16	21.708015660983186	24.61253373907349	29.04545592115581	24.633994678787513
17	21.529449636952435	27.087833022883416	28.26969797854456	23.113019361619585
18	21.526285524045882	25.209312947274874	28.78875006534581	24.475651463333435
19	22.658900374465883	26.59313084845	26.285936756261503	24.462032020822612
20	26.337250413398227	25.208900236895758	29.767836654734754	18.686012694971264
21	25.041339822974763	28.181653097666537	27.369163931313988	19.407843148044716
22	22.879287716913694	23.903359737626257	28.706070419396283	24.51128212606376
23	23.301077724369996	26.87377390624871	27.719692613309633	22.105455756071656
24	22.30589543019554	26.000753884292514	28.194171979166384	23.49917870634556
25	21.45873859199727	30.170779554878095	26.056744925725887	22.313736927398743
26	20.733193745511773	26.933479341094124	29.204486987241747	23.128839926152356
27	24.19831008856765	26.53810279790123	27.480183023296124	21.783404090235
28	20.41444376270804	26.94985018613238	30.50026000753884	22.135446043620735
29	22.313461787145997	23.90844983230202	31.574132413998036	22.203955966553952
30	23.289246693502015	26.929764947682084	28.281941719791664	21.49904663902424
31	25.709380356636796	25.34605765288856	24.890425394344767	24.054136596129876
32	25.662744083796717	26.633851605856083	26.899361949753885	20.80404236059331
33	26.243014876833463	25.005846730370806	24.892488946240345	23.858649446555383
34	23.884650200439676	24.845852673400266	28.075311389981046	23.19418573617902
35	24.41677144924625	24.10737623503581	27.636462686854625	23.839389628863312
36	25.42791187807985	26.19720402475162	27.73510046746329	20.639783629705242
37	25.05358356422186	25.15868714077001	29.09484359652333	20.692885698484805
38	24.654355057490555	25.417456548475588	25.863321328046972	24.064867065986885
39	25.003370468096115	24.421723973795643	28.710059953061073	21.864845605047172
40	24.98961345545892	23.074774866488195	28.42171296818553	23.513898709867355
41	22.763591240634913	23.996632283306415	28.383193332801394	24.856583143257275
42	22.405771341941556	23.50413123089495	29.99524007362753	24.094857353535964
43	24.114942591986267	23.49615216356538	28.52612869410182	23.86277655034654
44	23.570027321427098	23.62904490564065	29.048069753556877	23.752858019375374
45	24.002135088361293	23.58516003532801	27.284558303595258	25.128146572715444
46	25.315517084833992	25.77417588615797	27.009830761230536	21.9004762677775
47	22.336160857997363	24.866763332608794	31.22883139680452	21.56824441258932
48	23.660273324327076	24.20408803387527	28.709372102429214	23.42626653936844
49	22.290350005915517	22.692192345047886	31.485674822740894	23.531782826295704
50	22.57236876497795	23.04258345691716	29.603027643341196	24.782020134763698
51	22.32295412586566	23.005439522796745	27.742116543908256	26.929489807429334
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	244.0
1	828.5
2	1413.0
3	2621.5
4	3830.0
5	2658.0
6	1486.0
7	1458.0
8	1430.0
9	1398.5
10	1367.0
11	1395.5
12	1424.0
13	1400.0
14	1376.0
15	1365.5
16	1355.0
17	1315.0
18	1275.0
19	1467.0
20	1659.0
21	2188.0
22	2717.0
23	2800.5
24	2884.0
25	3762.5
26	5262.5
27	5884.0
28	7280.5
29	8677.0
30	10276.5
31	11876.0
32	13102.0
33	14328.0
34	16231.5
35	18135.0
36	19846.0
37	21557.0
38	23404.0
39	25251.0
40	27666.5
41	30082.0
42	32748.5
43	35415.0
44	38125.0
45	40835.0
46	43973.0
47	47111.0
48	64666.5
49	82222.0
50	73539.5
51	64857.0
52	64716.5
53	64576.0
54	56784.5
55	48993.0
56	44277.0
57	39561.0
58	35765.0
59	31969.0
60	29618.0
61	27267.0
62	26292.5
63	25318.0
64	21863.0
65	18408.0
66	15474.5
67	12541.0
68	10888.0
69	9235.0
70	7755.0
71	6275.0
72	5111.0
73	3947.0
74	3183.0
75	1937.0
76	1455.0
77	1230.5
78	1006.0
79	681.0
80	356.0
81	258.0
82	160.0
83	108.5
84	57.0
85	34.5
86	12.0
87	12.5
88	13.0
89	7.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	726902.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.728708460375888
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.15936903169306	18.56567368501107
2	10.457930983896304	5.381381147668001
3	3.706936964856121	2.861241013493214
4	1.8557905077250525	1.9098837174716332
5	1.1637756314761325	1.4971221967769632
6	0.828895443663906	1.2795845528497538
7	0.6139936167409427	1.105808393316164
8	0.5226722051596318	1.0758144629515152
9	0.46180040027678704	1.069337507904571
>10	7.632993659475365	48.763380670378325
>50	0.5207536346830138	8.085134654214889
>100	0.06536430476829573	3.013509756801709
>500	0.0037814060609757854	0.6557392783225929
>1k	0.0043216069268294695	1.804742375798828
>5k	0.001620602597561051	2.9316465870407917
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCG	7488	1.0301251062729226	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGC	7212	0.9921557513942734	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTC	6393	0.8794858178956724	No Hit
GCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC	2719	0.37405317360524526	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGGTGGCTT	2157	0.2967387625842273	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2093	0.28793427449642456	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCT	1507	0.20731818044248057	No Hit
CCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC	1184	0.16288302962435103	No Hit
GAACTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCT	1165	0.16026919722328456	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTA	1144	0.1573802245694743	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1016	0.13977124839386876	No Hit
CTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGCT	891	0.12257498259737902	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTC	841	0.1156964762787831	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12725236689402422	0.0
2	0.0	0.0	0.0	0.7862132722155118	0.0
3	0.0	0.0	0.0	1.0240720207125582	0.0
4	0.0	0.0	0.0	1.459481470679679	0.0
5	0.0	0.0	0.0	3.1211910271260774	0.0
6	0.0	0.0	0.0	3.69155677106405	0.0
7	0.0	0.0	0.0	4.368952073319375	0.0
8	0.0	0.0	0.0	5.146498427573456	0.0
9	0.0	0.0	0.0	5.4519041081191135	0.0
10	0.0	0.0	0.0	7.2573744466241665	0.0
11	0.0	0.0	0.0	8.464965015916864	0.0
12	0.0	0.0	0.0	10.478578955622629	0.0
13	0.0	0.0	0.0	10.925957006584106	0.0
14	0.0	0.0	0.0	11.1170419121147	0.0
15	1.3757012637191808E-4	0.0	0.0	11.553139212713681	0.0
16	1.3757012637191808E-4	0.0	0.0	12.17426833328289	0.0
17	1.3757012637191808E-4	0.0	0.0	12.861981395016109	0.0
18	1.3757012637191808E-4	0.0	0.0	13.550244737254816	0.0
19	1.3757012637191808E-4	0.0	0.0	14.31499706975631	0.0
20	1.3757012637191808E-4	0.0	0.0	14.789614005739425	0.0
21	1.3757012637191808E-4	0.0	0.0	15.322285535051492	0.0
22	2.7514025274383617E-4	0.0	0.0	15.921678575653939	0.0
23	2.7514025274383617E-4	0.0	0.0	16.45008543104848	0.0
24	4.1271037911575425E-4	0.0	0.0	16.922913955388758	0.0
25	4.1271037911575425E-4	0.0	0.0	17.32104190110909	0.0
26	4.1271037911575425E-4	0.0	0.0	17.69688348635717	0.0
27	4.1271037911575425E-4	0.0	0.0	18.107943023956462	0.0
28	4.1271037911575425E-4	0.0	0.0	18.473329279600275	0.0
29	4.1271037911575425E-4	0.0	0.0	18.85164712712305	0.0
30	4.1271037911575425E-4	0.0	0.0	19.301914150738337	0.0
31	4.1271037911575425E-4	0.0	0.0	19.682157980030322	0.0
32	4.1271037911575425E-4	0.0	0.0	20.084137889289064	0.0
33	4.1271037911575425E-4	0.0	0.0	20.450487135817482	0.0
34	4.1271037911575425E-4	0.0	0.0	20.837334331175317	0.0
35	4.1271037911575425E-4	0.0	0.0	21.266277985202958	0.0
36	4.1271037911575425E-4	0.0	0.0	21.652024619549817	0.0
37	4.1271037911575425E-4	0.0	0.0	22.048089013374568	0.0
38	4.1271037911575425E-4	0.0	0.0	22.43411078797417	0.0
39	4.1271037911575425E-4	0.0	0.0	22.823159105353955	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATGCG	35	1.2111013E-7	45.000004	1
CGCATCG	25	3.8893864E-5	45.0	21
ATCGTGT	20	7.0315733E-4	45.0	42
CGTAAGC	25	3.8893864E-5	45.0	43
TAGGCCG	25	3.8893864E-5	45.0	29
GAACCCG	20	7.0315733E-4	45.0	45
GACGCAC	25	3.8893864E-5	45.0	33
CGCCCAC	20	7.0315733E-4	45.0	34
AATCGTC	25	3.8893864E-5	45.0	40
AGGTCGA	25	3.8893864E-5	45.0	11
ATCCCGT	30	2.1643973E-6	44.999996	28
CGAAAGG	80	0.0	42.1875	2
GCTACGG	140	0.0	41.785717	2
CGGGTAT	65	0.0	41.53846	6
TACGAGG	65	0.0	41.53846	2
CGTAAGG	120	0.0	41.249996	2
TACGGCT	850	0.0	40.764706	7
CCGATGA	910	0.0	40.54945	18
ACGGCTG	860	0.0	40.2907	8
CTACGGG	215	0.0	39.767445	3
>>END_MODULE