FastQCFastQC Report
Sat 18 Jun 2016
SRR3551182_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551182_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences521084
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC16040.30781985246140736No Hit
CCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC11580.22222904560493126No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9730.18672613244697592No Hit
CTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGCT9410.1805850880088431No Hit
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA6520.12512378042695613No Hit
TCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC6150.11802319779536505No Hit
ATCCGCTAGTTCTGGCCCCTGGCCTCTGGCATAATATCTTCACAATGGTGC5250.10075151031311651No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGC453.8380676E-1045.0000046
GCATAAC253.88786E-545.09
GTTTCGA207.0297334E-445.023
CGCATTG253.88786E-545.018
TTTCGAA207.0297334E-445.024
CTAAGTC253.88786E-545.029
ATATGCG253.88786E-545.01
TAACGAC253.88786E-545.032
ACGATAG207.0297334E-445.01
TTAACGA253.88786E-545.031
TCGAGTA207.0297334E-445.043
TCATACG207.0297334E-445.018
ATACGTA207.0297334E-445.020
ACGAGTC253.88786E-545.040
CGTCCAA253.88786E-545.037
TTCGAAC207.0297334E-445.025
ATACCGG502.1827873E-1145.02
TGCAACG207.0297334E-445.012
TACGAAT1050.042.8571412
CGAATAT1050.042.8571414