##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551182_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	521084
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.3523673726309	33.0	31.0	34.0	30.0	34.0
2	32.44699702926975	34.0	31.0	34.0	31.0	34.0
3	32.4714997965779	34.0	31.0	34.0	30.0	34.0
4	35.96814717012996	37.0	35.0	37.0	35.0	37.0
5	36.0139344136454	37.0	35.0	37.0	35.0	37.0
6	36.044547520169495	37.0	35.0	37.0	35.0	37.0
7	36.181176163536016	37.0	37.0	37.0	35.0	37.0
8	35.867844723691384	37.0	36.0	37.0	35.0	37.0
9	37.73486040638362	39.0	38.0	39.0	35.0	39.0
10	37.608161064243	39.0	37.0	39.0	35.0	39.0
11	37.53923551673051	39.0	37.0	39.0	35.0	39.0
12	37.34362789876488	39.0	37.0	39.0	35.0	39.0
13	37.276228016979985	39.0	37.0	39.0	34.0	39.0
14	38.5166192015107	40.0	38.0	41.0	34.0	41.0
15	38.61679306983135	40.0	38.0	41.0	35.0	41.0
16	38.669212641339975	40.0	38.0	41.0	35.0	41.0
17	38.66339783988762	40.0	38.0	41.0	35.0	41.0
18	38.667297403105835	40.0	38.0	41.0	35.0	41.0
19	38.66617090526671	40.0	38.0	41.0	35.0	41.0
20	38.59968450384199	40.0	38.0	41.0	35.0	41.0
21	38.52662718486847	40.0	37.0	41.0	35.0	41.0
22	38.51282134934099	40.0	37.0	41.0	35.0	41.0
23	38.48837999247722	40.0	37.0	41.0	35.0	41.0
24	38.44204389311512	40.0	37.0	41.0	35.0	41.0
25	38.355789085828775	40.0	37.0	41.0	34.0	41.0
26	38.34313277705706	40.0	37.0	41.0	34.0	41.0
27	38.30284368739013	40.0	37.0	41.0	34.0	41.0
28	38.23453608247423	40.0	37.0	41.0	34.0	41.0
29	38.218709459511324	40.0	37.0	41.0	34.0	41.0
30	38.13342570487676	40.0	36.0	41.0	34.0	41.0
31	38.09397525159091	40.0	36.0	41.0	34.0	41.0
32	38.01496687674156	40.0	36.0	41.0	34.0	41.0
33	37.98778507879727	40.0	36.0	41.0	34.0	41.0
34	37.924428690959616	40.0	36.0	41.0	34.0	41.0
35	37.83285036577596	40.0	36.0	41.0	34.0	41.0
36	37.79852384644318	40.0	36.0	41.0	34.0	41.0
37	37.74036047930852	40.0	36.0	41.0	33.0	41.0
38	37.6579591774071	40.0	36.0	41.0	33.0	41.0
39	37.590369307059895	40.0	35.0	41.0	33.0	41.0
40	37.50865695358138	40.0	35.0	41.0	33.0	41.0
41	37.43147937760514	40.0	35.0	41.0	33.0	41.0
42	37.38721779981731	40.0	35.0	41.0	33.0	41.0
43	37.32428168970838	40.0	35.0	41.0	33.0	41.0
44	37.26118053903018	40.0	35.0	41.0	33.0	41.0
45	37.170696087387064	39.0	35.0	41.0	33.0	41.0
46	37.03868857996024	39.0	35.0	41.0	32.0	41.0
47	36.98250378058048	39.0	35.0	41.0	32.0	41.0
48	36.926343929193756	39.0	35.0	41.0	32.0	41.0
49	36.81036646682684	39.0	35.0	41.0	32.0	41.0
50	36.71577902986851	39.0	35.0	41.0	32.0	41.0
51	35.88156228170506	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	4.0
10	5.0
11	9.0
12	10.0
13	13.0
14	17.0
15	26.0
16	31.0
17	64.0
18	103.0
19	152.0
20	270.0
21	430.0
22	678.0
23	1028.0
24	1500.0
25	2071.0
26	3083.0
27	3547.0
28	4157.0
29	4659.0
30	5682.0
31	7145.0
32	9562.0
33	13515.0
34	27654.0
35	46033.0
36	31710.0
37	48623.0
38	90469.0
39	218365.0
40	463.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.44523339807018	21.273729379524223	22.51748278588481	11.763554436520792
2	32.07045313231648	25.045865925647306	24.474748792900954	18.408932149135264
3	32.53333435684074	23.995555419087903	26.06969317806726	17.4014170460041
4	29.29489295391914	26.21266436889254	25.16734346093912	19.325099216249203
5	26.236460916090305	30.581825578985345	23.273598882329914	19.90811462259444
6	26.118821533572323	34.86136592180915	23.588327409784217	15.431485134834308
7	78.72320009825671	7.070836947593861	9.295430295307474	4.9105326588419524
8	79.11277260480077	6.57763431615632	8.738514327824305	5.5710787512186135
9	72.777517636312	9.011406990043831	11.362083656377859	6.848991717266315
10	42.457262168863366	23.921670978191617	18.70504563563648	14.916021217308534
11	32.17312371901651	25.418742467625183	23.51501869180401	18.8931151215543
12	28.872504241158815	23.464163167550723	27.89281574563794	19.770516845652526
13	24.24081338133583	26.533917756062365	28.6959492135625	20.52931964903931
14	19.785293733831782	29.989598605982913	28.709574655909602	21.515533004275703
15	20.18177491536873	26.958993175764366	33.237251575561714	19.621980333305185
16	23.02546230550161	26.068733639873802	30.052544311473774	20.85325974315082
17	22.191239800109003	25.913288452533568	27.772489656178273	24.122982091179157
18	23.217945667109333	25.857827144951678	29.40082597047693	21.52340121746206
19	24.56878353585986	26.935196628566604	27.00351574794083	21.492504087632707
20	26.12457876273307	26.68552479062877	27.094479968680673	20.09541647795749
21	24.435407726969164	27.709160135410034	27.67288959169731	20.1825425459235
22	22.930659931987933	25.685877900683955	26.951892593132776	24.43156957419533
23	22.16302937722133	27.142264970714898	27.5350999071167	23.159605744947072
24	21.866915890720115	26.505707333174687	29.020081215312693	22.6072955607925
25	22.59885162469007	26.473274942235804	27.73698674302032	23.190886690053812
26	21.220762871245327	28.20927144184047	27.574824788325873	22.99514089858833
27	22.74681241412133	27.869019198440174	27.59420745983373	21.789960927604763
28	21.08738706235463	27.015605929178406	29.430379746835445	22.466627261631523
29	22.38410697699411	25.776650213785107	28.698444012865487	23.14079879635529
30	22.67216034267028	26.0090503642407	27.822769457515488	23.496019835573534
31	24.27190241880388	26.67247507119773	26.078329021808383	22.977293488190004
32	24.643627514949603	27.36065586354599	26.41013732910625	21.585579292398155
33	22.670241266283362	27.421874400288633	25.508171427255487	24.399712906172518
34	22.09758887242748	26.344313009035012	28.431884302722786	23.126213815814726
35	22.165716084163016	26.27119619869349	27.91680420047439	23.646283516669097
36	23.677756369414528	27.78592319088669	25.823859492903257	22.712460946795527
37	22.478141719953022	27.755985599250792	28.29870040147078	21.467172279325407
38	21.656777026352756	28.482931734614763	26.544664583829096	23.315626655203385
39	22.647404257279057	25.609882475762063	28.59519770324938	23.147515563709497
40	22.912428706312227	25.13606251583238	29.724190341672358	22.227318436183033
41	20.627576360049435	27.33359688649047	27.109640672137314	24.929186081322783
42	22.564883972641645	26.667677380230444	27.551412056405493	23.216026590722414
43	23.319272900338525	24.9311051577097	27.512262898112393	24.237359043839383
44	22.43611394707955	25.72675422772528	27.512838621028475	24.3242932041667
45	22.126183110592535	25.1907561928595	26.97971920074307	25.7033414958049
46	22.609214637179416	26.352181222221372	27.46985130996154	23.56875283063767
47	21.772497332483823	25.858210960229062	29.24634032133015	23.122951385956966
48	21.655625580520606	25.441963291906873	28.671768851087347	24.230642276485174
49	22.257447935457623	24.881209171649868	29.312932271956154	23.548410620936355
50	20.979151154132538	24.75896400580329	29.029292781969897	25.232592058094284
51	20.534693062922678	25.182312256757065	27.20290778454146	27.0800868957788
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	183.0
1	561.0
2	939.0
3	1441.5
4	1944.0
5	1422.0
6	900.0
7	886.5
8	873.0
9	855.5
10	838.0
11	857.0
12	876.0
13	944.5
14	1013.0
15	1022.0
16	1031.0
17	1038.5
18	1046.0
19	1090.5
20	1135.0
21	1899.5
22	2664.0
23	2382.5
24	2101.0
25	2935.0
26	4441.0
27	5113.0
28	5481.5
29	5850.0
30	7116.0
31	8382.0
32	9749.0
33	11116.0
34	11866.5
35	12617.0
36	14161.0
37	15705.0
38	17318.0
39	18931.0
40	21348.0
41	23765.0
42	26893.5
43	30022.0
44	33214.0
45	36406.0
46	40645.0
47	44884.0
48	48138.0
49	51392.0
50	49986.0
51	48580.0
52	43339.0
53	38098.0
54	33555.0
55	29012.0
56	26991.0
57	24970.0
58	23991.5
59	23013.0
60	21765.0
61	20517.0
62	18168.5
63	15820.0
64	13943.5
65	12067.0
66	10340.0
67	8613.0
68	7392.5
69	6172.0
70	5112.5
71	4053.0
72	3512.0
73	2971.0
74	2315.5
75	1383.5
76	1107.0
77	853.0
78	599.0
79	393.0
80	187.0
81	150.5
82	114.0
83	71.5
84	29.0
85	17.5
86	6.0
87	3.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	521084.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.271739135203067
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.82453933583292	19.58780100272967
2	10.80500551482753	5.893425835096138
3	3.951625803394225	3.233031242103136
4	1.89646679598472	2.0687979095467863
5	1.1571222733032254	1.5778368392529354
6	0.835669004489518	1.3674088256287786
7	0.588495074024241	1.1234498898798784
8	0.44123737869529445	0.9626648550783101
9	0.45033881481598237	1.1053370412106194
>10	7.553853888436509	51.90318377779748
>50	0.4325380985399907	7.063909866119684
>100	0.05743538685531024	2.7616331352639056
>500	0.004254473100393351	0.8174093734864876
>1k	0.001418157700131117	0.5341104068061695
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC	1604	0.30781985246140736	No Hit
CCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC	1158	0.22222904560493126	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	973	0.18672613244697592	No Hit
CTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGCT	941	0.1805850880088431	No Hit
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA	652	0.12512378042695613	No Hit
TCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC	615	0.11802319779536505	No Hit
ATCCGCTAGTTCTGGCCCCTGGCCTCTGGCATAATATCTTCACAATGGTGC	525	0.10075151031311651	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.919076386916505E-4	0.0	0.0	0.18730185536305088	0.0
2	3.83815277383301E-4	0.0	0.0	0.94879136569152	0.0
3	3.83815277383301E-4	0.0	0.0	1.2825187493763002	0.0
4	3.83815277383301E-4	0.0	0.0	1.7267849329474712	0.0
5	5.757229160749514E-4	0.0	0.0	2.9642053872312335	0.0
6	5.757229160749514E-4	0.0	0.0	3.6596786698497747	0.0
7	7.67630554766602E-4	0.0	0.0	4.325214360832419	0.0
8	7.67630554766602E-4	0.0	0.0	5.3384866931243335	0.0
9	7.67630554766602E-4	0.0	0.0	5.764713558658489	0.0
10	7.67630554766602E-4	0.0	0.0	6.74037199376684	0.0
11	7.67630554766602E-4	0.0	0.0	8.059545102133246	0.0
12	7.67630554766602E-4	0.0	0.0	9.049020887227396	0.0
13	7.67630554766602E-4	0.0	0.0	9.4717934152651	0.0
14	7.67630554766602E-4	0.0	0.0	9.638753060926836	0.0
15	9.595381934582524E-4	0.0	0.0	9.9751671515533	0.0
16	9.595381934582524E-4	0.0	0.0	10.655095915437817	0.0
17	9.595381934582524E-4	0.0	0.0	11.431938036861618	0.0
18	9.595381934582524E-4	0.0	0.0	12.312026467901529	0.0
19	9.595381934582524E-4	0.0	0.0	12.836893859723192	0.0
20	9.595381934582524E-4	0.0	0.0	13.324723077277367	0.0
21	9.595381934582524E-4	0.0	0.0	13.954563947463365	0.0
22	0.0011514458321499029	0.0	0.0	14.579990941959453	0.0
23	0.0011514458321499029	0.0	0.0	15.181621389257778	0.0
24	0.0011514458321499029	0.0	0.0	15.692479523454951	0.0
25	0.0011514458321499029	0.0	0.0	16.178581572260903	0.0
26	0.0011514458321499029	0.0	0.0	16.617474341948707	0.0
27	0.0011514458321499029	0.0	0.0	17.04350929984417	0.0
28	0.0011514458321499029	0.0	0.0	17.463978936217575	0.0
29	0.0011514458321499029	0.0	0.0	17.919183855194174	0.0
30	0.0011514458321499029	0.0	0.0	18.420446607456764	0.0
31	0.0011514458321499029	0.0	0.0	18.90155905765673	0.0
32	0.0011514458321499029	0.0	0.0	19.38535821479838	0.0
33	0.0011514458321499029	0.0	0.0	19.857451005979843	0.0
34	0.0011514458321499029	0.0	0.0	20.299606205525404	0.0
35	0.0011514458321499029	0.0	0.0	20.773042350177708	0.0
36	0.0011514458321499029	0.0	0.0	21.22229813235486	0.0
37	0.0011514458321499029	0.0	0.0	21.667140038842106	0.0
38	0.0011514458321499029	0.0	0.0	22.09106401271196	0.0
39	0.0011514458321499029	0.0	0.0	22.550874715017155	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGGCGC	45	3.8380676E-10	45.000004	6
GCATAAC	25	3.88786E-5	45.0	9
GTTTCGA	20	7.0297334E-4	45.0	23
CGCATTG	25	3.88786E-5	45.0	18
TTTCGAA	20	7.0297334E-4	45.0	24
CTAAGTC	25	3.88786E-5	45.0	29
ATATGCG	25	3.88786E-5	45.0	1
TAACGAC	25	3.88786E-5	45.0	32
ACGATAG	20	7.0297334E-4	45.0	1
TTAACGA	25	3.88786E-5	45.0	31
TCGAGTA	20	7.0297334E-4	45.0	43
TCATACG	20	7.0297334E-4	45.0	18
ATACGTA	20	7.0297334E-4	45.0	20
ACGAGTC	25	3.88786E-5	45.0	40
CGTCCAA	25	3.88786E-5	45.0	37
TTCGAAC	20	7.0297334E-4	45.0	25
ATACCGG	50	2.1827873E-11	45.0	2
TGCAACG	20	7.0297334E-4	45.0	12
TACGAAT	105	0.0	42.85714	12
CGAATAT	105	0.0	42.85714	14
>>END_MODULE