FastQCFastQC Report
Sat 18 Jun 2016
SRR3551180_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551180_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1215761
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54870.45132225823990074No Hit
GCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC23640.1944461123526746TruSeq Adapter, Index 15 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC22870.18811263068974907TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCT16950.139418849592971TruSeq Adapter, Index 14 (95% over 23bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG12830.10553061004588894No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTTA351.2120108E-745.00000440
CGGTATT351.2120108E-745.00000443
TAACGCC207.0334476E-445.012
GCGATAC253.890939E-545.09
TACGTTG302.165607E-644.9999961
CGAATAT1100.040.90909214
ACCGGTA403.4584446E-739.37541
TCGACGT800.039.37526
GACCGAC356.248587E-638.571439
ATACCGG356.248587E-638.571432
TTAAGCG659.094947E-1238.076921
CGTAAGG1800.037.52
TTACGGG3300.037.53
CTTAACG301.1399609E-437.4999961
ATTAGCG301.1399609E-437.4999961
CGGCATA301.1399609E-437.49999611
ATAGACG301.1399609E-437.4999961
TTAGTCG301.1399609E-437.49999641
CGGTCTA601.5643309E-1037.49999631
AGTACGG1200.037.4999962
Produced by FastQC (version 0.11.5)