##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551180_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1215761
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.39865976947772	33.0	31.0	34.0	30.0	34.0
2	32.504841000821706	34.0	31.0	34.0	31.0	34.0
3	32.513635492502225	34.0	31.0	34.0	31.0	34.0
4	36.00233105026399	37.0	35.0	37.0	35.0	37.0
5	36.040060505313136	37.0	35.0	37.0	35.0	37.0
6	36.06810302353834	37.0	36.0	37.0	35.0	37.0
7	36.19725093994626	37.0	37.0	37.0	35.0	37.0
8	35.89084367733461	37.0	36.0	37.0	35.0	37.0
9	37.74762556127397	39.0	38.0	39.0	35.0	39.0
10	37.63481391490597	39.0	37.0	39.0	35.0	39.0
11	37.546880513521984	39.0	37.0	39.0	35.0	39.0
12	37.383794183231736	39.0	37.0	39.0	35.0	39.0
13	37.3313537775928	39.0	37.0	39.0	35.0	39.0
14	38.586897424740556	40.0	38.0	41.0	35.0	41.0
15	38.6640976310311	40.0	38.0	41.0	35.0	41.0
16	38.706065583613885	40.0	38.0	41.0	35.0	41.0
17	38.702747497246584	40.0	38.0	41.0	35.0	41.0
18	38.68155500957836	40.0	38.0	41.0	35.0	41.0
19	38.648518088670386	40.0	38.0	41.0	35.0	41.0
20	38.57303450266952	40.0	38.0	41.0	35.0	41.0
21	38.50862052656731	40.0	37.0	41.0	35.0	41.0
22	38.47407097283101	40.0	37.0	41.0	35.0	41.0
23	38.459319718267	40.0	37.0	41.0	35.0	41.0
24	38.401694905495404	40.0	37.0	41.0	34.0	41.0
25	38.2930954356983	40.0	37.0	41.0	34.0	41.0
26	38.258278559684015	40.0	37.0	41.0	34.0	41.0
27	38.22611598825756	40.0	37.0	41.0	34.0	41.0
28	38.14459667648494	40.0	36.0	41.0	34.0	41.0
29	38.10343069073609	40.0	36.0	41.0	34.0	41.0
30	37.99492087671837	40.0	36.0	41.0	34.0	41.0
31	37.95608923135386	40.0	36.0	41.0	34.0	41.0
32	37.85402394056069	40.0	36.0	41.0	34.0	41.0
33	37.81511744495835	40.0	36.0	41.0	34.0	41.0
34	37.708514255680186	40.0	36.0	41.0	33.0	41.0
35	37.59452556875899	40.0	35.0	41.0	33.0	41.0
36	37.547985993957695	40.0	35.0	41.0	33.0	41.0
37	37.52021984584141	40.0	35.0	41.0	33.0	41.0
38	37.4281885995685	40.0	35.0	41.0	33.0	41.0
39	37.38783280595446	40.0	35.0	41.0	33.0	41.0
40	37.27150731105867	40.0	35.0	41.0	33.0	41.0
41	37.160609692201014	39.0	35.0	41.0	33.0	41.0
42	37.1223225617535	39.0	35.0	41.0	33.0	41.0
43	37.08111956215078	39.0	35.0	41.0	33.0	41.0
44	37.020192291083525	39.0	35.0	41.0	33.0	41.0
45	36.94593345238086	39.0	35.0	41.0	32.0	41.0
46	36.81340493731909	39.0	35.0	41.0	32.0	41.0
47	36.76734078490756	39.0	35.0	41.0	32.0	41.0
48	36.7060590033732	39.0	35.0	41.0	32.0	41.0
49	36.626332807188255	39.0	35.0	41.0	32.0	41.0
50	36.56194021686828	39.0	35.0	41.0	32.0	41.0
51	35.777747435556826	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	13.0
10	21.0
11	22.0
12	22.0
13	35.0
14	55.0
15	61.0
16	88.0
17	170.0
18	262.0
19	420.0
20	658.0
21	1095.0
22	1593.0
23	2382.0
24	3566.0
25	5264.0
26	7588.0
27	9619.0
28	10437.0
29	11882.0
30	14039.0
31	17236.0
32	22881.0
33	32502.0
34	68100.0
35	102114.0
36	79899.0
37	120081.0
38	214632.0
39	488095.0
40	926.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.811671866427695	21.42263158630685	22.787620264180212	13.978076283085244
2	33.04300763061161	25.436660659455274	23.848190557190104	17.672141152743016
3	32.479903533671504	24.35478683721554	25.610050001603934	17.555259627509027
4	29.876184546140237	25.787963259226114	25.484367404448736	18.851484790184912
5	25.672397782129874	30.372416947080882	24.094620570983935	19.86056469980531
6	26.761016351075583	33.826796549650794	23.878212905332543	15.53397419394108
7	78.89947119540766	6.546845967258368	9.463619905557096	5.090062931776887
8	79.40911083675162	6.160421332811301	9.177708447630744	5.252759382806325
9	72.73650001932945	8.513926668152704	11.872728274718467	6.87684503779937
10	43.127802257187064	23.500589342806684	18.46349734857427	14.908111051431982
11	31.71371675847473	25.55658554600781	22.77692737306099	19.95277032245647
12	29.440079094493075	22.55895690024602	27.142670311023302	20.858293694237602
13	25.6246087841278	23.601678290387667	29.000519016484326	21.773193909000206
14	21.44360610350225	26.45380136391939	29.20055833342244	22.902034199155917
15	21.36966064876238	25.91052024205415	31.700227265062786	21.019591844120676
16	24.41285746129379	24.913778283725176	29.97217380718743	20.701190447793604
17	23.823514654607276	25.07968260209038	27.086162494108628	24.010640249193717
18	24.202043000227842	25.480007994992437	28.21524954329017	22.102699461489554
19	26.072394162997497	26.030445128606694	26.311092393982037	21.58606831441377
20	26.55637086565534	25.867501918551426	27.15722909354717	20.418898122246066
21	26.142309220315507	26.041220272734527	27.528107909367055	20.288362597582914
22	23.682779756876556	24.346561536354596	27.826275065576212	24.144383641192636
23	24.039840067250058	24.905059464812574	28.41150522183225	22.643595246105114
24	24.59233352607955	24.428074267886537	28.393656318964005	22.585935887069912
25	24.35610288535329	25.390927986668434	27.044131206709217	23.208837921269065
26	22.31688629590849	26.78396494047761	26.989926474035602	23.909222289578295
27	23.280398038759262	25.87490468932627	27.71235464865216	23.132342623262304
28	21.28132091751586	26.477983748450555	28.523287060532454	23.71740827350112
29	23.29528583331757	26.001574322584787	27.114293023053047	23.588846821044594
30	22.56323405669371	26.128572967877734	26.84647722702077	24.461715748407787
31	23.615825807868486	26.66979776452773	24.973905233018662	24.74047119458512
32	23.60439263967178	27.06173335055163	26.0191764664272	23.314697543349393
33	23.28936361669769	25.86890021969779	26.00675626212718	24.834979901477347
34	22.205351216234114	25.218278921597253	27.747065418285338	24.829304443883295
35	22.527865262991657	24.814169890299162	28.142949148722487	24.51501569798669
36	22.219580986723543	26.68855145049068	26.72992471382122	24.361942848964556
37	23.31083165194475	26.07946792173791	27.451365852334465	23.15833457398288
38	22.029905548870214	26.116070510569102	27.125314926206713	24.72870901435397
39	23.793492306464838	23.975435961508882	27.848072112857707	24.38299961916857
40	24.38744128163348	23.26082182271022	29.055217267209592	23.29651962844671
41	20.987430917754395	25.019308893771065	28.582838238765678	25.410421949708866
42	21.11780193640033	25.708753611935244	28.957007174929938	24.21643727673449
43	22.3903382325967	24.030545477277197	28.641320127886978	24.937796162239124
44	22.52720723892278	24.544709034094694	27.01805700298003	25.9100267240025
45	23.037833916370076	24.31333132087639	26.56344462439575	26.085390138357784
46	22.70405120743304	25.591131809623768	27.034178592667473	24.67063839027572
47	22.203294891018878	24.816143962505787	29.087295940567266	23.89326520590807
48	21.408895333869076	23.84218608756162	29.262412595896727	25.48650598267258
49	22.162086133705554	23.86028174945569	29.221861862652283	24.755770254186473
50	21.01087302520808	23.631618385521495	29.406602119989046	25.95090646928138
51	21.06376170974394	23.82302113655562	28.03009802091036	27.083119132790078
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	642.0
1	1546.0
2	2450.0
3	5833.0
4	9216.0
5	6143.5
6	3071.0
7	2917.5
8	2764.0
9	2725.5
10	2687.0
11	2753.5
12	2820.0
13	2737.0
14	2654.0
15	2615.0
16	2576.0
17	2496.5
18	2417.0
19	2544.0
20	2671.0
21	3708.5
22	4746.0
23	4730.0
24	4714.0
25	5479.5
26	8314.5
27	10384.0
28	12155.5
29	13927.0
30	15568.5
31	17210.0
32	19275.5
33	21341.0
34	24439.0
35	27537.0
36	29564.0
37	31591.0
38	33407.0
39	35223.0
40	40140.5
41	45058.0
42	51524.5
43	57991.0
44	65131.0
45	72271.0
46	84449.5
47	96628.0
48	103507.5
49	110387.0
50	108799.0
51	107211.0
52	98736.5
53	90262.0
54	81323.0
55	72384.0
56	68385.0
57	64386.0
58	62327.5
59	60269.0
60	59895.0
61	59521.0
62	53925.5
63	48330.0
64	44534.5
65	40739.0
66	34205.0
67	27671.0
68	24113.0
69	20555.0
70	16965.0
71	13375.0
72	11186.0
73	8997.0
74	7365.0
75	4620.5
76	3508.0
77	2615.5
78	1723.0
79	1451.5
80	1180.0
81	796.5
82	413.0
83	298.0
84	183.0
85	127.0
86	71.0
87	47.0
88	23.0
89	12.5
90	2.0
91	2.0
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1215761.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.64758728216396
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.62199887631648	17.6530946862706
2	10.517465047475834	5.184602754895389
3	3.845295995564265	2.8433180602927743
4	1.9427078027531182	1.9153224052839382
5	1.1813980272732607	1.4559305496097008
6	0.7847493597215661	1.1605307003015752
7	0.6084665783250723	1.0498063168282814
8	0.4994364886795504	0.9847923557301371
9	0.448454743591509	0.994799469129496
>10	7.592579549283221	48.00412520716396
>50	0.8748973892968659	12.981575387918411
>100	0.07375381514264137	3.3499372714180167
>500	0.006089764553052567	1.0641114559661282
>1k	0.002368241770631554	0.9005046323155097
>5k	3.3832025294736485E-4	0.4575487468761183
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5487	0.45132225823990074	No Hit
GCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	2364	0.1944461123526746	TruSeq Adapter, Index 15 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	2287	0.18811263068974907	TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCT	1695	0.139418849592971	TruSeq Adapter, Index 14 (95% over 23bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	1283	0.10553061004588894	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.645060172188448E-4	0.0	0.0	0.1450120541784117	0.0
2	1.645060172188448E-4	0.0	0.0	0.6701975141495738	0.0
3	1.645060172188448E-4	0.0	0.0	0.9489529603269063	0.0
4	1.645060172188448E-4	0.0	0.0	1.325671739758061	0.0
5	1.645060172188448E-4	0.0	0.0	2.384350213569937	0.0
6	1.645060172188448E-4	0.0	0.0	3.2738342486722307	0.0
7	1.645060172188448E-4	0.0	0.0	3.9642660029397225	0.0
8	1.645060172188448E-4	0.0	0.0	4.914123746361333	0.0
9	1.645060172188448E-4	0.0	0.0	5.36297841434295	0.0
10	1.645060172188448E-4	0.0	0.0	6.177036440550404	0.0
11	1.645060172188448E-4	0.0	0.0	7.268040346745783	0.0
12	1.645060172188448E-4	0.0	0.0	8.100769805907575	0.0
13	1.645060172188448E-4	0.0	0.0	8.47757083834734	0.0
14	1.645060172188448E-4	0.0	0.0	8.64709428909136	0.0
15	1.645060172188448E-4	0.0	0.0	8.942300336990577	0.0
16	1.645060172188448E-4	0.0	0.0	9.484594422752497	0.0
17	1.645060172188448E-4	0.0	0.0	10.1009162162629	0.0
18	1.645060172188448E-4	0.0	0.0	10.793980066805894	0.0
19	1.645060172188448E-4	0.0	0.0	11.22589061501397	0.0
20	1.645060172188448E-4	0.0	0.0	11.665203933996896	0.0
21	1.645060172188448E-4	0.0	0.0	12.189073345830307	0.0
22	1.645060172188448E-4	0.0	0.0	12.736055853082966	0.0
23	1.645060172188448E-4	0.0	0.0	13.264366927381285	0.0
24	1.645060172188448E-4	0.0	0.0	13.712892583328467	0.0
25	1.645060172188448E-4	0.0	0.0	14.126542963625251	0.0
26	1.645060172188448E-4	0.0	0.0	14.525881320423998	0.0
27	2.4675902582826725E-4	0.0	0.0	14.908275557449203	0.0
28	3.290120344376896E-4	0.0	0.0	15.299635372412835	0.0
29	3.290120344376896E-4	0.0	0.0	15.698480211159923	0.0
30	3.290120344376896E-4	0.0	0.0	16.139603096332255	0.0
31	4.1126504304711206E-4	0.0	0.0	16.553006717603214	0.0
32	4.1126504304711206E-4	0.0	0.0	16.991579759508653	0.0
33	4.1126504304711206E-4	0.0	0.0	17.401364248400796	0.0
34	4.935180516565345E-4	0.0	0.0	17.820525580274413	0.0
35	4.935180516565345E-4	0.0	0.0	18.250708815301692	0.0
36	4.935180516565345E-4	0.0	0.0	18.6479908468852	0.0
37	4.935180516565345E-4	0.0	0.0	19.066329648672724	0.0
38	4.935180516565345E-4	0.0	0.0	19.46254239114431	0.0
39	5.757710602659569E-4	0.0	0.0	19.880963445940445	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCGTTA	35	1.2120108E-7	45.000004	40
CGGTATT	35	1.2120108E-7	45.000004	43
TAACGCC	20	7.0334476E-4	45.0	12
GCGATAC	25	3.890939E-5	45.0	9
TACGTTG	30	2.165607E-6	44.999996	1
CGAATAT	110	0.0	40.909092	14
ACCGGTA	40	3.4584446E-7	39.375	41
TCGACGT	80	0.0	39.375	26
GACCGAC	35	6.248587E-6	38.57143	9
ATACCGG	35	6.248587E-6	38.57143	2
TTAAGCG	65	9.094947E-12	38.07692	1
CGTAAGG	180	0.0	37.5	2
TTACGGG	330	0.0	37.5	3
CTTAACG	30	1.1399609E-4	37.499996	1
ATTAGCG	30	1.1399609E-4	37.499996	1
CGGCATA	30	1.1399609E-4	37.499996	11
ATAGACG	30	1.1399609E-4	37.499996	1
TTAGTCG	30	1.1399609E-4	37.499996	41
CGGTCTA	60	1.5643309E-10	37.499996	31
AGTACGG	120	0.0	37.499996	2
>>END_MODULE