FastQCFastQC Report
Sat 18 Jun 2016
SRR3551176_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551176_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences736696
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTC121001.642468535189549No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84031.140633314148577No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGC52010.7059899877289953No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCG51150.6943162444210367No Hit
GAACTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCT35970.4882611009154387No Hit
GCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCTGC28920.39256355403042775TruSeq Adapter, Index 14 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTT21230.2881785702650754No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCT12890.17497040841812633No Hit
CGCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCTG12430.16872631316038095TruSeq Adapter, Index 21 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTC11950.16221073550012488No Hit
GAATATGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTC11810.1603103586825502No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTT11630.15786701705995418No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCAGGTTT9380.1273252467775039No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9350.12691802317373788No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTC8580.11646595067707712No Hit
CCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCTGC8330.11307242064569376TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCTGCT8000.1085929610042677TruSeq Adapter, Index 21 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTCGA207.0316356E-445.012
ACGTAAG207.0316356E-445.01
CGCAACA351.2111377E-745.043
CGTTATT4700.043.0851061
CGTTTTT34350.042.7729231
CGATGAA10950.042.12328719
CTCGAAT750.042.043
CCGATGA10800.041.66666818
TAATACG2650.041.6037754
CGAATGC1600.040.7812545
TACGGCT7500.039.8999987
ATAATAC3750.039.63
CGTTCTG1550.039.193551
GCGATAT356.2452364E-638.5714269
GCGTATG659.094947E-1238.076921
GCGTAAG659.094947E-1238.076921
AATACGG2900.038.0172425
GATGAAT12550.037.82868620
GAATCTG17400.037.51
TCGAATG1800.037.544
Produced by FastQC (version 0.11.5)