##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551176_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	736696
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.973390652317917	33.0	31.0	34.0	30.0	34.0
2	32.094158241662775	33.0	31.0	34.0	30.0	34.0
3	32.004374938916456	33.0	31.0	34.0	30.0	34.0
4	35.67286099015062	37.0	35.0	37.0	33.0	37.0
5	32.02139281331784	37.0	35.0	37.0	0.0	37.0
6	33.77319002682246	37.0	35.0	37.0	17.0	37.0
7	35.37620809669117	37.0	35.0	37.0	32.0	37.0
8	35.57728968258277	37.0	35.0	37.0	33.0	37.0
9	37.49822179026356	39.0	37.0	39.0	35.0	39.0
10	37.225205512178704	39.0	37.0	39.0	34.0	39.0
11	37.20883376589529	39.0	37.0	39.0	34.0	39.0
12	37.158320392672145	39.0	37.0	39.0	34.0	39.0
13	37.11116932900409	39.0	37.0	39.0	33.0	39.0
14	38.341708112980115	40.0	38.0	41.0	33.0	41.0
15	38.426830062875325	40.0	38.0	41.0	34.0	41.0
16	38.41836388415303	40.0	38.0	41.0	34.0	41.0
17	38.35438226894133	40.0	38.0	41.0	34.0	41.0
18	38.22602403162227	40.0	37.0	41.0	34.0	41.0
19	38.054508236776094	40.0	37.0	41.0	34.0	41.0
20	37.93175203883284	40.0	36.0	41.0	33.0	41.0
21	37.95021284220357	40.0	36.0	41.0	33.0	41.0
22	37.998672451051725	40.0	36.0	41.0	34.0	41.0
23	37.9654090696841	40.0	36.0	41.0	34.0	41.0
24	37.95913375395007	40.0	36.0	41.0	33.0	41.0
25	37.73471282591463	40.0	36.0	41.0	33.0	41.0
26	37.69472889767285	40.0	35.0	41.0	33.0	41.0
27	37.66497714118171	40.0	36.0	41.0	33.0	41.0
28	37.55721763115315	40.0	35.0	41.0	33.0	41.0
29	37.45789579419462	40.0	35.0	41.0	33.0	41.0
30	37.307204057033026	40.0	35.0	41.0	32.0	41.0
31	37.27434111220911	40.0	35.0	41.0	32.0	41.0
32	37.01948157720417	40.0	35.0	41.0	32.0	41.0
33	36.88687192546179	40.0	35.0	41.0	31.0	41.0
34	36.58386362895957	40.0	35.0	41.0	30.0	41.0
35	36.40840726704095	39.0	35.0	41.0	30.0	41.0
36	36.28630398427574	39.0	35.0	41.0	30.0	41.0
37	36.210533245735014	39.0	35.0	41.0	30.0	41.0
38	36.11727089600052	39.0	35.0	41.0	29.0	41.0
39	36.00297544713152	39.0	35.0	41.0	29.0	41.0
40	35.86967487267475	39.0	35.0	41.0	28.0	41.0
41	35.746218250133026	39.0	35.0	41.0	27.0	41.0
42	35.681790046369194	39.0	35.0	41.0	27.0	41.0
43	35.66240348800591	39.0	35.0	41.0	27.0	41.0
44	35.68656270700533	39.0	35.0	41.0	27.0	41.0
45	35.68724955748368	39.0	35.0	41.0	28.0	41.0
46	35.56222105183142	38.0	35.0	40.0	27.0	41.0
47	35.38732394366197	38.0	35.0	40.0	27.0	41.0
48	35.37745827315474	38.0	35.0	40.0	27.0	41.0
49	35.32276814316896	38.0	35.0	40.0	27.0	41.0
50	35.209247776559124	38.0	35.0	40.0	26.0	41.0
51	34.224411426151356	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	22.0
9	35.0
10	47.0
11	35.0
12	24.0
13	14.0
14	21.0
15	33.0
16	76.0
17	110.0
18	250.0
19	513.0
20	1073.0
21	2105.0
22	3325.0
23	4556.0
24	5946.0
25	7489.0
26	9320.0
27	10043.0
28	10029.0
29	10571.0
30	11960.0
31	14979.0
32	19105.0
33	27020.0
34	43033.0
35	55239.0
36	59671.0
37	88280.0
38	138156.0
39	213474.0
40	138.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.34191036736999	17.258136327603243	20.786185889430648	15.613767415596122
2	34.64536253760031	27.671386840704987	21.875780511907216	15.807470109787483
3	29.859942228544746	27.95440724532236	26.69839391010675	15.48725661602615
4	25.851233073072205	23.122020480632447	32.87122503719309	18.155521409102263
5	24.136821701217325	34.329221279876634	23.53508095605243	17.998876062853604
6	22.898861945768676	35.794547547427975	26.985622291963036	14.320968214840313
7	74.11768219184033	4.9512960569895865	16.53531443091859	4.395707320251501
8	73.8320827043991	8.054339917686535	12.841117638754657	5.272459739159708
9	68.26682919413165	5.855196716148859	14.53381041840868	11.344163671310826
10	38.32232019720482	23.845385342122125	21.272275131126	16.56001932954706
11	28.215980540141388	22.253005310195793	28.839711359909654	20.69130278975317
12	23.343414379879896	19.094850521789176	34.60816944845635	22.953565649874573
13	23.812535971418335	20.94988977814458	36.38203546646107	18.855538783976023
14	19.276743731471328	27.163443265607523	32.85371442223115	20.70609858069
15	17.445459185335608	23.22817010001412	36.704556560643745	22.621814154006536
16	20.539136903145938	23.377213938992476	31.4580233909238	24.625625766937787
17	21.304988760628536	24.418484693822144	30.471320598998773	23.805205946550544
18	21.97541455362863	23.02143625050224	30.74429615472325	24.258853041145873
19	21.2379326072084	25.00855169567909	28.160733871230466	25.592781825882042
20	24.319529358107008	25.00787298967281	29.874873760682835	20.797723891537352
21	23.229120288422905	26.814859860783823	29.913152779436835	20.042867071356437
22	22.131245452669756	21.176441843039733	31.44200592917567	25.25030677511484
23	22.78063135947528	24.569836133221845	30.243411122091068	22.40612138521181
24	22.578784193208595	24.462736325431386	29.692437586195663	23.266041895164356
25	21.492990324367174	28.041281614125772	27.884093303072095	22.58163475843496
26	20.69469631978455	23.293461617817933	30.607740506260384	25.40410155613713
27	24.513910758304647	22.63308067371073	28.736276564553087	24.116732003431537
28	20.169377870926404	25.157459793456187	29.980751897661996	24.692410437955413
29	22.01830877322532	25.481609782053926	29.1246321413446	23.375449303376154
30	24.780506477570125	25.524639742851868	28.399638385439857	21.295215394138154
31	25.297273230749184	23.82923213917274	25.657123155277073	25.216371474801
32	25.1565096050474	27.448364047042475	26.067333065470695	21.327793282439433
33	26.913679455297707	22.51905806465625	26.52220183087732	24.04506064916872
34	25.20564791990183	24.100443059280895	27.991464593265064	22.70244442755221
35	25.370709220628317	24.38753569993593	29.02024715757924	21.221507921856507
36	24.53630805651178	26.05810266378533	28.675600247591955	20.729989032110936
37	23.999994570351948	25.04235125479167	28.927128693518085	22.0305254813383
38	22.65697112513167	24.98221790263555	27.274208085831873	25.086602886400904
39	24.724309620250416	25.04995276206196	27.21936864052472	23.0063689771629
40	24.575944487278335	21.876051994309726	30.75013302637723	22.797870492034704
41	22.04301367185379	24.50047237938037	29.28779306525351	24.168720883512332
42	21.959668574283015	24.344098515534224	31.341014475441703	22.355218434741058
43	23.19342035249275	22.84022174682637	29.6488646605927	24.31749324008818
44	23.664035097244994	22.415216045695917	28.65646073821495	25.264288118844135
45	23.428116889463226	21.836551304744425	27.735049464093738	27.00028234169861
46	25.83657302333663	23.40273328482848	27.66894349908239	23.091750192752507
47	21.82854257387036	23.819458772682356	31.577611389229748	22.774387264217534
48	21.42498398253825	24.2022489602224	29.6133004658638	24.75946659137555
49	22.375443873728106	21.86356380379424	32.84176919652068	22.919223125956975
50	22.319654240012163	21.879852747944877	32.33341839781945	23.467074614223506
51	22.17020317743004	21.60429810939655	29.372631316038095	26.852867397135316
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	282.0
1	725.0
2	1168.0
3	7414.0
4	13660.0
5	8583.5
6	3507.0
7	3362.0
8	3217.0
9	3268.0
10	3319.0
11	3215.0
12	3111.0
13	3077.5
14	3044.0
15	2869.0
16	2694.0
17	2531.0
18	2368.0
19	2323.0
20	2278.0
21	2458.0
22	2638.0
23	2717.5
24	2797.0
25	3097.0
26	4106.0
27	4815.0
28	5802.0
29	6789.0
30	7868.0
31	8947.0
32	10110.5
33	11274.0
34	13133.5
35	14993.0
36	16443.5
37	17894.0
38	19485.5
39	21077.0
40	23250.5
41	25424.0
42	27625.5
43	29827.0
44	35595.5
45	41364.0
46	62135.5
47	82907.0
48	73429.0
49	63951.0
50	63594.5
51	63238.0
52	57517.5
53	51797.0
54	48436.0
55	45075.0
56	42993.0
57	40911.0
58	39549.0
59	38187.0
60	35113.5
61	32040.0
62	28394.5
63	24749.0
64	22031.0
65	19313.0
66	17072.5
67	14832.0
68	12581.5
69	10331.0
70	8581.0
71	6831.0
72	6241.5
73	5652.0
74	4773.0
75	2818.5
76	1743.0
77	1237.5
78	732.0
79	501.0
80	270.0
81	247.0
82	224.0
83	150.5
84	77.0
85	54.0
86	31.0
87	22.5
88	14.0
89	12.0
90	10.0
91	6.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	736696.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.41470673130353
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.25857013136596	25.590160700256536
2	8.963666202196995	6.348912195762077
3	3.8468049232660793	4.087004046300083
4	2.302841557404524	3.2621783361655803
5	1.7123066004818641	3.032041804507027
6	1.357915892688686	2.8854115863207634
7	1.1523041370758165	2.856595915586548
8	1.034277251894924	2.9302900443773825
9	0.9439170460035735	3.008569082660541
>10	6.343600761897069	35.43406759952413
>50	0.04404135394853641	1.0785474919687228
>100	0.030789972552033206	2.0791093730839756
>500	0.004287211368004619	1.1265810730340169
>1k	0.0031179719040033598	2.0266588943100308
>5k	0.0011692394640012598	2.5837381899195986
>10k+	3.8974648800041997E-4	1.6701336662229362
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTC	12100	1.642468535189549	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8403	1.140633314148577	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGC	5201	0.7059899877289953	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCG	5115	0.6943162444210367	No Hit
GAACTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCT	3597	0.4882611009154387	No Hit
GCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCTGC	2892	0.39256355403042775	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTT	2123	0.2881785702650754	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCT	1289	0.17497040841812633	No Hit
CGCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCTG	1243	0.16872631316038095	TruSeq Adapter, Index 21 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTC	1195	0.16221073550012488	No Hit
GAATATGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTC	1181	0.1603103586825502	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTT	1163	0.15786701705995418	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCAGGTTT	938	0.1273252467775039	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	935	0.12691802317373788	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTC	858	0.11646595067707712	No Hit
CCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCTGC	833	0.11307242064569376	TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCTGCT	800	0.1085929610042677	TruSeq Adapter, Index 21 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.072236037660039E-4	0.0	0.0	0.11782336268963046	0.0
2	4.072236037660039E-4	0.0	0.0	0.7127770477917621	0.0
3	4.072236037660039E-4	0.0	0.0	1.0692334422882708	0.0
4	4.072236037660039E-4	0.0	0.0	1.8992908879646422	0.0
5	4.072236037660039E-4	0.0	0.0	4.342225286956899	0.0
6	4.072236037660039E-4	0.0	0.0	5.721898856516121	0.0
7	4.072236037660039E-4	0.0	0.0	6.645346248656162	0.0
8	4.072236037660039E-4	0.0	0.0	7.928643565324096	0.0
9	4.072236037660039E-4	0.0	0.0	8.52902689847644	0.0
10	4.072236037660039E-4	0.0	0.0	10.155342230716604	0.0
11	4.072236037660039E-4	0.0	0.0	11.062364937504752	0.0
12	4.072236037660039E-4	0.0	0.0	12.620538186714738	0.0
13	4.072236037660039E-4	0.0	0.0	13.065226362027213	0.0
14	4.072236037660039E-4	0.0	0.0	13.32340612681486	0.0
15	4.072236037660039E-4	0.0	0.0	13.660858753135622	0.0
16	4.072236037660039E-4	0.0	0.0	14.089936690303734	0.0
17	4.072236037660039E-4	0.0	0.0	14.584713368879429	0.0
18	4.072236037660039E-4	0.0	0.0	15.12442038507064	0.0
19	4.072236037660039E-4	0.0	0.0	15.611867038778547	0.0
20	4.072236037660039E-4	0.0	0.0	15.947419288281733	0.0
21	4.072236037660039E-4	0.0	0.0	16.304011423979496	0.0
22	4.072236037660039E-4	0.0	0.0	16.740555127216656	0.0
23	4.072236037660039E-4	0.0	0.0	17.15062929620902	0.0
24	4.072236037660039E-4	0.0	0.0	17.49161119376242	0.0
25	5.429648050213385E-4	0.0	0.0	17.784947929675198	0.0
26	5.429648050213385E-4	0.0	0.0	18.07353372354404	0.0
27	5.429648050213385E-4	0.0	0.0	18.412343761877356	0.0
28	5.429648050213385E-4	0.0	0.0	18.698757696526112	0.0
29	5.429648050213385E-4	0.0	0.0	19.01096245941338	0.0
30	6.787060062766731E-4	0.0	0.0	19.44601300943673	0.0
31	6.787060062766731E-4	0.0	0.0	19.802740886335748	0.0
32	8.144472075320078E-4	0.0	0.0	20.138564618241446	0.0
33	9.501884087873424E-4	0.0	0.0	20.497871577964315	0.0
34	9.501884087873424E-4	0.0	0.0	20.884869742743277	0.0
35	9.501884087873424E-4	0.0	0.0	21.251506727333933	0.0
36	0.001085929610042677	0.0	0.0	21.591809918881058	0.0
37	0.001085929610042677	0.0	0.0	21.919353437510182	0.0
38	0.001085929610042677	0.0	0.0	22.277438726421753	0.0
39	0.001085929610042677	0.0	0.0	22.737329916274827	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTTCGA	20	7.0316356E-4	45.0	12
ACGTAAG	20	7.0316356E-4	45.0	1
CGCAACA	35	1.2111377E-7	45.0	43
CGTTATT	470	0.0	43.085106	1
CGTTTTT	3435	0.0	42.772923	1
CGATGAA	1095	0.0	42.123287	19
CTCGAAT	75	0.0	42.0	43
CCGATGA	1080	0.0	41.666668	18
TAATACG	265	0.0	41.603775	4
CGAATGC	160	0.0	40.78125	45
TACGGCT	750	0.0	39.899998	7
ATAATAC	375	0.0	39.6	3
CGTTCTG	155	0.0	39.19355	1
GCGATAT	35	6.2452364E-6	38.571426	9
GCGTATG	65	9.094947E-12	38.07692	1
GCGTAAG	65	9.094947E-12	38.07692	1
AATACGG	290	0.0	38.017242	5
GATGAAT	1255	0.0	37.828686	20
GAATCTG	1740	0.0	37.5	1
TCGAATG	180	0.0	37.5	44
>>END_MODULE