FastQCFastQC Report
Sat 18 Jun 2016
SRR3551174_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551174_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences337568
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCG13620.40347426296331407No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGC12990.384811356526685No Hit
GAATCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTTC12610.3735543653426865No Hit
GCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTTCTGC11330.3356360792492179No Hit
CCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTTCTGC7190.21299412266565554No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5740.17003981420039813No Hit
CTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTTCTGCT5400.15996776945682056No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGGT207.0262E-445.04
TTTACGG551.8189894E-1245.02
CGACGGT207.0262E-445.028
TCCGATT207.0262E-445.014
ACGCAAC207.0262E-445.040
TAAACGG207.0262E-445.02
CCAGCGA207.0262E-445.014
GTTAGCG207.0262E-445.01
ATCCGTC207.0262E-445.040
CGTTAGG253.8849324E-545.02
CTACGAA207.0262E-445.011
CGGTCTA207.0262E-445.031
AGGGCGC406.7902874E-945.06
CCACGCA207.0262E-445.038
GCGTCTC207.0262E-445.022
AGTCGTT207.0262E-445.018
TGCGTAG351.2085184E-745.01
CGGACGG253.8849324E-545.02
TAACGGG950.040.2631573
ACGGGCG451.9213985E-840.05