##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551174_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	337568
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.0715055929472	33.0	31.0	34.0	30.0	34.0
2	32.18211145606219	33.0	31.0	34.0	30.0	34.0
3	32.14419613233482	33.0	31.0	34.0	30.0	34.0
4	35.75764290454072	37.0	35.0	37.0	35.0	37.0
5	32.08062671817234	37.0	35.0	37.0	0.0	37.0
6	33.86441546592094	37.0	35.0	37.0	17.0	37.0
7	35.50719262489336	37.0	35.0	37.0	32.0	37.0
8	35.73628424495213	37.0	35.0	37.0	35.0	37.0
9	37.66308714096123	39.0	37.0	39.0	35.0	39.0
10	37.30979239738364	39.0	37.0	39.0	34.0	39.0
11	37.269068750592474	39.0	37.0	39.0	34.0	39.0
12	37.13945930893924	39.0	37.0	39.0	34.0	39.0
13	37.05294340695801	39.0	37.0	39.0	33.0	39.0
14	38.24035453597497	40.0	37.0	41.0	33.0	41.0
15	38.3797931083515	40.0	37.0	41.0	34.0	41.0
16	38.342188833064746	40.0	37.0	41.0	34.0	41.0
17	38.3566955398616	40.0	37.0	41.0	34.0	41.0
18	38.29351123329226	40.0	37.0	41.0	34.0	41.0
19	38.16044174803299	40.0	37.0	41.0	34.0	41.0
20	38.23017584605176	40.0	37.0	41.0	34.0	41.0
21	38.18875308086074	40.0	37.0	41.0	34.0	41.0
22	38.23112380320409	40.0	37.0	41.0	34.0	41.0
23	38.244883993743485	40.0	37.0	41.0	34.0	41.0
24	38.209525191961326	40.0	37.0	41.0	34.0	41.0
25	37.9468018295573	40.0	36.0	41.0	34.0	41.0
26	37.98502227699308	40.0	36.0	41.0	34.0	41.0
27	37.964774504692386	40.0	36.0	41.0	34.0	41.0
28	37.890771637122	40.0	36.0	41.0	34.0	41.0
29	37.86424957341928	40.0	36.0	41.0	34.0	41.0
30	37.7277555929472	40.0	36.0	41.0	33.0	41.0
31	37.712771945208075	40.0	36.0	41.0	33.0	41.0
32	37.6340144800455	40.0	36.0	41.0	33.0	41.0
33	37.59982285050716	40.0	36.0	41.0	33.0	41.0
34	37.49723907479382	40.0	35.0	41.0	33.0	41.0
35	37.493435396720066	40.0	35.0	41.0	33.0	41.0
36	37.408933903687554	40.0	35.0	41.0	33.0	41.0
37	37.308089036875536	40.0	35.0	41.0	33.0	41.0
38	37.18738743008816	40.0	35.0	41.0	33.0	41.0
39	37.14592615413783	39.0	35.0	41.0	33.0	41.0
40	37.034476016684046	39.0	35.0	41.0	32.0	41.0
41	36.93566037065125	39.0	35.0	41.0	32.0	41.0
42	36.9071120485354	39.0	35.0	41.0	32.0	41.0
43	36.855774244004174	39.0	35.0	41.0	32.0	41.0
44	36.79105543179448	39.0	35.0	41.0	32.0	41.0
45	36.77418179448289	39.0	35.0	41.0	32.0	41.0
46	36.66790394824154	39.0	35.0	41.0	31.0	41.0
47	36.56889871077827	39.0	35.0	41.0	31.0	41.0
48	36.54771779315575	39.0	35.0	41.0	31.0	41.0
49	36.53702365153095	39.0	35.0	41.0	31.0	41.0
50	36.420119798085125	39.0	35.0	41.0	31.0	41.0
51	35.41452981325244	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	3.0
10	15.0
11	11.0
12	12.0
13	12.0
14	13.0
15	21.0
16	22.0
17	31.0
18	68.0
19	151.0
20	294.0
21	495.0
22	746.0
23	990.0
24	1343.0
25	1660.0
26	2088.0
27	2541.0
28	2931.0
29	3611.0
30	4406.0
31	6067.0
32	7981.0
33	12206.0
34	21569.0
35	29930.0
36	26129.0
37	39468.0
38	64215.0
39	108453.0
40	81.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.711181154611815	19.658853919802823	22.623589913735902	11.006375011849464
2	33.96945208076595	24.650440800075838	23.552884159635983	17.827222959522228
3	33.879988624514176	25.083538724049674	24.303843966252725	16.73262868518343
4	29.344013650582994	25.261873163333014	26.197980851265523	19.196132334818465
5	23.751955161626697	37.11252251398236	21.134704711347048	18.00081761304389
6	25.882192624893356	35.96193952033368	23.79935301924353	14.356514835529435
7	78.71332590766897	6.087069864442127	10.25126789269125	4.9483363351976495
8	79.44947388378046	6.845435586311499	8.65869987676557	5.0463906531424785
9	73.26908948715518	7.777692198312636	10.66185183429709	8.291366480235093
10	41.00566404398521	24.566902076026164	18.465612854298985	15.961821025689638
11	32.3161555597687	25.51160062565172	23.034470091951846	19.13777372262774
12	28.26897099251114	22.120876386387337	29.563524978670962	20.04662764243056
13	25.55929471987866	24.832922551900655	30.887702625841314	18.72008010237937
14	22.123838752488385	28.815231301545168	28.786200113754855	20.274729832211584
15	20.52801213385155	25.10723765285809	34.32997203526401	20.034778178026354
16	22.381564603279934	23.825125604322682	31.79685278225424	21.996457010143143
17	22.701796378803678	23.465494359654944	28.83507915442222	24.99763010711916
18	23.564733624040194	23.52326049862546	30.434756848990425	22.47724902834392
19	24.34324343539672	26.64885297184567	27.01796378803678	21.989939804720827
20	25.128862925395772	25.617357095459287	28.737024836477392	20.51675514266755
21	24.691025215660254	27.11601810598161	28.087674187126744	20.105282491231396
22	22.348386102948147	24.750272537681298	28.19787420608588	24.703467153284674
23	21.671781685467817	27.262062754763484	27.755000473978576	23.311155085790123
24	22.860875438430185	24.783747274623185	29.715494359654944	22.639882927291684
25	21.08434448762916	26.631671248459572	28.312517774196603	23.971466489714665
26	20.89771542326287	26.84496160773533	29.010451227604513	23.24687174139729
27	23.242131955635607	26.423120674945494	27.736930040762157	22.597817328656745
28	20.528308370461655	26.270855057351405	30.772466584510383	22.428369987676557
29	21.52099725092426	25.76192056119063	29.431699687174138	23.28538250071097
30	23.496895440326096	25.954770594369137	29.126872215375865	21.421461749928902
31	25.508934496160773	26.717579865390086	25.121753246753247	22.651732391695894
32	24.797374158688026	28.148106455588206	25.12797421556546	21.92654517015831
33	24.992297848137266	25.206773153853447	26.99574604227889	22.8051829557304
34	22.282325338894683	27.67057304009859	27.221182102568964	22.825919518437768
35	22.37800976395867	27.17289553512181	26.44326476443265	24.005829936486872
36	25.98350554554934	27.526009574367237	26.036828135368285	20.45365674471514
37	23.446238979998103	27.717378424495216	26.86895677315385	21.96742582235283
38	22.452068916484976	27.874976301071193	26.08600341264575	23.586951369798083
39	23.84408474736942	26.232936771257936	26.796378803678074	23.126599677694568
40	22.77170821878851	26.78008579012229	28.304519385723765	22.14368660536544
41	22.091252725376815	27.84624134989098	26.45244809934591	23.610057825386292
42	23.64975353114039	26.540134135937056	26.791342781306287	23.018769551616266
43	25.051248933548205	24.143283723575696	26.843184188074698	23.962283154801405
44	22.686392075078206	25.099239264385247	28.21861076879325	23.995757891743295
45	22.78296520997251	25.447613517868994	26.708100293866714	25.06132097829178
46	23.612427718267135	27.112167030050244	27.283095554080955	21.99230969760167
47	22.03319034979619	26.474962081713905	29.355566878377097	22.136280690112805
48	22.463918380889183	25.407917812114896	28.534695231775526	23.5934685752204
49	23.101419565835624	24.02271542326287	29.823324485733245	23.052540525168265
50	21.886553227794103	24.200161152715896	29.428441084462982	24.484844535027015
51	21.695480614276235	24.643331121433313	27.171414352071288	26.489773912219167
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	122.0
1	380.5
2	639.0
3	909.0
4	1179.0
5	852.5
6	526.0
7	495.0
8	464.0
9	511.5
10	559.0
11	565.0
12	571.0
13	566.5
14	562.0
15	604.5
16	647.0
17	625.0
18	603.0
19	709.5
20	816.0
21	909.0
22	1002.0
23	1159.5
24	1317.0
25	1575.0
26	2181.0
27	2529.0
28	3271.5
29	4014.0
30	4426.0
31	4838.0
32	5450.0
33	6062.0
34	7158.5
35	8255.0
36	8716.0
37	9177.0
38	10351.0
39	11525.0
40	13183.5
41	14842.0
42	16897.5
43	18953.0
44	21318.0
45	23683.0
46	28587.5
47	33492.0
48	33985.5
49	34479.0
50	33619.0
51	32759.0
52	29520.5
53	26282.0
54	22843.5
55	19405.0
56	17900.5
57	16396.0
58	15499.0
59	14602.0
60	14022.0
61	13442.0
62	11558.5
63	9675.0
64	8479.5
65	7284.0
66	6320.5
67	5357.0
68	4438.5
69	3520.0
70	2944.0
71	2368.0
72	2007.0
73	1646.0
74	1319.5
75	782.0
76	571.0
77	440.0
78	309.0
79	226.5
80	144.0
81	115.5
82	87.0
83	52.0
84	17.0
85	11.5
86	6.0
87	4.0
88	2.0
89	1.0
90	0.0
91	1.0
92	2.0
93	4.5
94	7.0
95	5.0
96	3.0
97	2.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	337568.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.14829979016197
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.39669615908156	25.446207804166658
2	9.966570438072297	7.006160112742621
3	3.9114507360611026	4.1244252925657605
4	2.026176989119376	2.848667049659823
5	1.3499984576052728	2.3725075252083196
6	1.012627199000028	2.135527461967499
7	0.8263085065022246	2.033033737399083
8	0.7882005676479559	2.2163127877172952
9	0.6010966573067161	1.901477296249264
>10	7.015047482534868	44.57577674404002
>50	0.07026899989354325	1.6214621299502747
>100	0.029631505979204983	1.6687758533237549
>500	0.0025398433696461418	0.5454468864376681
>1k	0.003386457826194856	1.5042193185719652
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCG	1362	0.40347426296331407	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGC	1299	0.384811356526685	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTTC	1261	0.3735543653426865	No Hit
GCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTTCTGC	1133	0.3356360792492179	No Hit
CCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTTCTGC	719	0.21299412266565554	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	574	0.17003981420039813	No Hit
CTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTTCTGCT	540	0.15996776945682056	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.924732202104465E-4	0.0	0.0	0.16618873826903025	0.0
2	5.924732202104465E-4	0.0	0.0	0.8952270357379847	0.0
3	5.924732202104465E-4	0.0	0.0	1.1852426770309983	0.0
4	5.924732202104465E-4	0.0	0.0	1.6325599582898853	0.0
5	5.924732202104465E-4	0.0	0.0	3.0796757986539007	0.0
6	5.924732202104465E-4	0.0	0.0	3.7894587164660156	0.0
7	5.924732202104465E-4	0.0	0.0	4.524421746137075	0.0
8	5.924732202104465E-4	0.0	0.0	5.6471584984358705	0.0
9	5.924732202104465E-4	0.0	0.0	6.009159635984454	0.0
10	5.924732202104465E-4	0.0	0.0	7.294234050620912	0.0
11	5.924732202104465E-4	0.0	0.0	8.566274054412741	0.0
12	5.924732202104465E-4	0.0	0.0	9.97724902834392	0.0
13	5.924732202104465E-4	0.0	0.0	10.427528675703858	0.0
14	5.924732202104465E-4	0.0	0.0	10.611787847189307	0.0
15	8.887098303156697E-4	0.0	0.0	11.0084486681202	0.0
16	8.887098303156697E-4	0.0	0.0	11.738671912029575	0.0
17	8.887098303156697E-4	0.0	0.0	12.569319366764622	0.0
18	8.887098303156697E-4	0.0	0.0	13.47372973741587	0.0
19	8.887098303156697E-4	0.0	0.0	14.041319082377477	0.0
20	8.887098303156697E-4	0.0	0.0	14.560029386671722	0.0
21	8.887098303156697E-4	0.0	0.0	15.180052611621955	0.0
22	8.887098303156697E-4	0.0	0.0	15.821701109109869	0.0
23	8.887098303156697E-4	0.0	0.0	16.47105175846052	0.0
24	8.887098303156697E-4	0.0	0.0	16.972580339368662	0.0
25	8.887098303156697E-4	0.0	0.0	17.41101052232439	0.0
26	8.887098303156697E-4	0.0	0.0	17.874028343918855	0.0
27	8.887098303156697E-4	0.0	0.0	18.295276803488484	0.0
28	0.001184946440420893	0.0	0.0	18.765108067115367	0.0
29	0.0014811830505261161	0.0	0.0	19.210647928713623	0.0
30	0.0014811830505261161	0.0	0.0	19.713953929282397	0.0
31	0.0014811830505261161	0.0	0.0	20.21163143425917	0.0
32	0.0014811830505261161	0.0	0.0	20.66191108161911	0.0
33	0.0014811830505261161	0.0	0.0	21.12522513982368	0.0
34	0.0014811830505261161	0.0	0.0	21.59090909090909	0.0
35	0.0017774196606313395	0.0	0.0	22.075552185041236	0.0
36	0.0017774196606313395	0.0	0.0	22.532645274433595	0.0
37	0.0017774196606313395	0.0	0.0	22.969298037728695	0.0
38	0.0017774196606313395	0.0	0.0	23.403580908142953	0.0
39	0.0017774196606313395	0.0	0.0	23.831939046355103	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGGT	20	7.0262E-4	45.0	4
TTTACGG	55	1.8189894E-12	45.0	2
CGACGGT	20	7.0262E-4	45.0	28
TCCGATT	20	7.0262E-4	45.0	14
ACGCAAC	20	7.0262E-4	45.0	40
TAAACGG	20	7.0262E-4	45.0	2
CCAGCGA	20	7.0262E-4	45.0	14
GTTAGCG	20	7.0262E-4	45.0	1
ATCCGTC	20	7.0262E-4	45.0	40
CGTTAGG	25	3.8849324E-5	45.0	2
CTACGAA	20	7.0262E-4	45.0	11
CGGTCTA	20	7.0262E-4	45.0	31
AGGGCGC	40	6.7902874E-9	45.0	6
CCACGCA	20	7.0262E-4	45.0	38
GCGTCTC	20	7.0262E-4	45.0	22
AGTCGTT	20	7.0262E-4	45.0	18
TGCGTAG	35	1.2085184E-7	45.0	1
CGGACGG	25	3.8849324E-5	45.0	2
TAACGGG	95	0.0	40.263157	3
ACGGGCG	45	1.9213985E-8	40.0	5
>>END_MODULE