FastQCFastQC Report
Sat 18 Jun 2016
SRR3551173_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551173_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189463
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2550.13459092276592263No Hit
CCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGC2270.11981231163868407No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACGA406.7630026E-945.024
CGACGGT406.7630026E-945.028
TAGCACG253.8784397E-545.01
CGCAGGA207.0183614E-445.04
CTATTGG207.0183614E-445.02
CGGTCTA406.7630026E-945.031
GCTACGG207.0183614E-445.02
GTAAGGC207.0183614E-445.04
TAGGAAC207.0183614E-445.06
ACGGTCT406.7630026E-945.030
GTTAGGA556.002665E-1140.9090924
GTAGGGT501.0713848E-940.54
TACGGGC501.0713848E-940.54
TCGGGAA451.9137588E-840.05
ACGGGAC451.9137588E-840.05
CGGGCGA451.9137588E-840.06
AACGGGA850.039.7058834
CGGGATA403.4378718E-739.3756
TACGGGA750.039.04
ACGCCGG356.2207055E-638.57142627