##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551173_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	189463
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.997645978370446	33.0	31.0	34.0	30.0	34.0
2	32.13936230292986	33.0	31.0	34.0	30.0	34.0
3	32.044082485762395	33.0	31.0	34.0	30.0	34.0
4	35.78989565244929	37.0	35.0	37.0	35.0	37.0
5	35.85400315628909	37.0	35.0	37.0	35.0	37.0
6	35.83179829307041	37.0	35.0	37.0	35.0	37.0
7	26.923130109836748	36.0	10.0	37.0	0.0	37.0
8	31.243039538062842	36.0	19.0	37.0	17.0	37.0
9	36.02553532879771	39.0	32.0	39.0	32.0	39.0
10	36.683859117611355	38.0	35.0	39.0	33.0	39.0
11	37.1517077212965	39.0	37.0	39.0	34.0	39.0
12	37.15499068419692	39.0	37.0	39.0	34.0	39.0
13	37.09793996717037	39.0	37.0	39.0	33.0	39.0
14	38.242522286673385	40.0	37.0	41.0	34.0	41.0
15	38.32751513488122	40.0	38.0	41.0	34.0	41.0
16	38.26900766904356	40.0	37.0	41.0	34.0	41.0
17	38.16338810216243	40.0	37.0	41.0	34.0	41.0
18	38.09465172619456	40.0	37.0	41.0	33.0	41.0
19	37.998374352776004	40.0	37.0	41.0	34.0	41.0
20	38.077281580044655	40.0	37.0	41.0	34.0	41.0
21	38.027282371755966	40.0	37.0	41.0	34.0	41.0
22	38.03444472007727	40.0	37.0	41.0	34.0	41.0
23	37.95709980312779	40.0	37.0	41.0	34.0	41.0
24	37.952761225146865	40.0	37.0	41.0	33.0	41.0
25	37.848112824139804	40.0	36.0	41.0	33.0	41.0
26	37.74041369555005	40.0	36.0	41.0	33.0	41.0
27	37.569778795859875	40.0	36.0	41.0	33.0	41.0
28	37.560183254777975	40.0	36.0	41.0	33.0	41.0
29	37.53220417706888	40.0	36.0	41.0	33.0	41.0
30	37.34672732934663	40.0	36.0	41.0	32.0	41.0
31	37.25547996178673	39.0	36.0	41.0	32.0	41.0
32	37.10122820814618	39.0	35.0	41.0	31.0	41.0
33	36.99707594622697	39.0	35.0	41.0	31.0	41.0
34	36.83478568374828	39.0	35.0	41.0	31.0	41.0
35	36.69682734887551	39.0	35.0	41.0	30.0	41.0
36	36.51973208489257	39.0	35.0	41.0	30.0	41.0
37	36.45205660208062	39.0	35.0	41.0	30.0	41.0
38	36.307447892200585	39.0	35.0	41.0	30.0	41.0
39	36.27203728432465	39.0	35.0	41.0	30.0	41.0
40	36.09531676369529	39.0	35.0	40.0	29.0	41.0
41	35.95052332117616	39.0	35.0	40.0	28.0	41.0
42	35.8928075666489	39.0	35.0	40.0	28.0	41.0
43	35.827639169653175	39.0	35.0	40.0	28.0	41.0
44	35.60418129133393	38.0	35.0	40.0	27.0	41.0
45	35.450737083229974	38.0	34.0	40.0	26.0	41.0
46	35.246253885983016	38.0	34.0	40.0	25.0	41.0
47	35.10480146519373	38.0	34.0	40.0	24.0	41.0
48	35.01402912442007	38.0	34.0	40.0	24.0	41.0
49	34.842375556177196	38.0	34.0	40.0	23.0	41.0
50	34.62769511725244	38.0	34.0	40.0	23.0	41.0
51	32.25954407984673	35.0	30.0	39.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	4.0
10	12.0
11	9.0
12	6.0
13	5.0
14	4.0
15	7.0
16	22.0
17	25.0
18	64.0
19	107.0
20	195.0
21	334.0
22	556.0
23	841.0
24	1221.0
25	1526.0
26	1944.0
27	2312.0
28	2630.0
29	3181.0
30	3864.0
31	4829.0
32	6391.0
33	8380.0
34	13020.0
35	15912.0
36	17530.0
37	28199.0
38	36231.0
39	40090.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.46675076400142	23.182362783234723	27.861904435166764	12.488982017597102
2	30.065500915746085	26.011939006560645	26.851680803111954	17.070879274581316
3	31.060418129133392	24.543578429561443	28.00863493135863	16.387368509946533
4	29.33554308756855	25.391237339216627	27.279204910721354	17.99401466249347
5	25.328428241925867	30.792819706222325	24.682391812649435	19.196360239202377
6	26.68594923547078	34.74609818275864	24.061690145305416	14.506262436465168
7	63.74490005964225	27.269176567456444	5.3187165831851075	3.667206789716198
8	86.23319592743702	4.079424478658102	6.15951399481693	3.527865599087949
9	79.27880377699076	8.187350564490165	7.982033431329599	4.551812227189478
10	33.99027778510843	41.22915819975404	13.252191720810924	11.528372294326598
11	32.19837118593076	25.04130094002523	24.61641587011712	18.143912003926886
12	31.656840649625522	21.522935876661933	27.674532758374987	19.145690715337558
13	26.669587201722766	24.44962868739543	28.941270854995434	19.939513255886375
14	21.62533053947209	27.82654132996944	28.854710418393037	21.693417712165434
15	20.909623514881535	25.184864590975547	33.864131782986654	20.04138011115627
16	23.93237729794208	24.93679504705404	30.667729319181053	20.463098335822828
17	23.269978834917634	24.18414149464539	28.04294242147543	24.50293724896154
18	23.611998121005158	25.03338382692135	30.28401323741311	21.07060481466038
19	23.940294411045958	26.870154067021	27.349931121115993	21.839620400817044
20	25.386487071354303	27.091305426389322	27.21111773802801	20.31108976422837
21	24.26595166338546	26.960409156405206	29.15239387109884	19.621245309110485
22	24.063273567926192	23.455239281548376	28.623002908219547	23.858484242305884
23	21.936736988224613	26.570359384154163	28.92332539862664	22.56957822899458
24	22.85459430073418	24.882958677947673	30.10455867372521	22.157888347592934
25	21.36617703720515	26.923990436127372	28.224508215324363	23.48532431134311
26	21.30653478515594	27.770593730702036	27.898323155444597	23.024548328697424
27	21.561465827100808	27.498245039928644	28.50741305690293	22.432876076067622
28	19.30983886035796	26.669587201722766	31.45416255416625	22.566411383753028
29	22.27981188939265	24.826483271140013	29.74248270110787	23.15122213835947
30	22.364261095834014	25.423433599172395	30.14150520154331	22.070800103450278
31	23.874846276053898	25.300982249832423	28.434047808806994	22.39012366530668
32	24.547273082343253	25.467241625013852	27.59852847257776	22.386956820065134
33	23.580857476129903	24.60849875701324	29.596807819996517	22.213835946860335
34	22.920570243266493	23.758200809656767	30.742677989897764	22.578550957178976
35	21.30600697761568	24.75945171352718	29.83907148097518	24.09546982788196
36	23.037215709663627	25.790787647192325	30.507275826942465	20.66472081620158
37	22.05971614510485	26.23150694330819	29.67070087563271	22.038076035954248
38	19.982265666647315	27.775871806104625	28.806152124689255	23.43571040255881
39	22.823981463399186	24.57366345935618	30.04174957643445	22.560605500810187
40	23.452600243847083	23.68958582942316	29.766234040419505	23.091579886310257
41	20.80775665961164	24.8164549278751	29.71609232409494	24.659696088418322
42	21.557771174318997	24.157223310092206	30.009025508938418	24.275980006650375
43	21.879205966336436	24.317676802330798	30.11986509239271	23.683252138940055
44	21.16033209650433	24.70878218966236	29.48913508178378	24.64175063204953
45	22.239170708792745	24.55255115774584	28.413463314736916	24.7948148187245
46	22.480906562231148	24.957379541124126	29.236843077540204	23.32487081910452
47	20.90065078669714	24.461240453281118	31.084169468445026	23.553939291576718
48	21.31445189825982	23.1966135868217	31.466829935132452	24.022104579786028
49	21.901901690567552	22.5057135166233	31.622005351968458	23.97037944084069
50	20.704834189261227	23.305341940114957	31.312710133376964	24.67711373724685
51	20.549130964884966	23.070995392240174	29.461161282150076	26.918712360724783
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	74.0
1	191.0
2	308.0
3	496.5
4	685.0
5	510.5
6	336.0
7	289.0
8	242.0
9	249.5
10	257.0
11	281.5
12	306.0
13	290.0
14	274.0
15	321.5
16	369.0
17	355.0
18	341.0
19	389.5
20	438.0
21	521.0
22	604.0
23	792.5
24	981.0
25	1139.5
26	1479.5
27	1661.0
28	2082.5
29	2504.0
30	3067.0
31	3630.0
32	4061.5
33	4493.0
34	5073.5
35	5654.0
36	5998.5
37	6343.0
38	6819.0
39	7295.0
40	8311.5
41	9328.0
42	10842.0
43	12356.0
44	13578.5
45	14801.0
46	16275.5
47	17750.0
48	18194.5
49	18639.0
50	17778.0
51	16917.0
52	15036.5
53	13156.0
54	11789.0
55	10422.0
56	9619.5
57	8817.0
58	8241.0
59	7665.0
60	7243.0
61	6821.0
62	5973.0
63	5125.0
64	4294.0
65	3463.0
66	2811.0
67	2159.0
68	1843.0
69	1527.0
70	1214.5
71	902.0
72	778.0
73	654.0
74	508.5
75	279.5
76	196.0
77	180.0
78	164.0
79	117.0
80	70.0
81	51.5
82	33.0
83	25.5
84	18.0
85	18.0
86	18.0
87	10.5
88	3.0
89	2.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	189463.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.75774689517215
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.65079474934828	32.328211840834356
2	9.615428980090893	8.799607311190048
3	4.933444067640206	6.772298549057072
4	3.2805038410962695	6.004338577980925
5	2.448843057189656	5.602677039844192
6	1.8709483931990682	5.1366229817959175
7	1.418783306803239	4.544422921625858
8	1.1880868341523056	4.349134131730207
9	0.9793065264032113	4.032977415115353
>10	3.5954045262647933	21.622691501770795
>50	0.01384178835905599	0.40799522861983606
>100	0.004613929453018663	0.3990225004354412
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	255	0.13459092276592263	No Hit
CCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGC	227	0.11981231163868407	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09553316478679215	0.0
2	0.0	0.0	0.0	0.6233407050453122	0.0
3	0.0	0.0	0.0	0.8972728184394843	0.0
4	0.0	0.0	0.0	1.2181798029166644	0.0
5	0.0	0.0	0.0	2.188817869452083	0.0
6	0.0	0.0	0.0	2.6664836933860436	0.0
7	0.0	0.0	0.0	3.2022083467484417	0.0
8	0.0	0.0	0.0	4.233016472873332	0.0
9	0.0	0.0	0.0	4.615676939560759	0.0
10	0.0	0.0	0.0	5.5646748969455775	0.0
11	0.0	0.0	0.0	6.79077181296612	0.0
12	0.0	0.0	0.0	7.815774056148166	0.0
13	0.0	0.0	0.0	8.233269820492655	0.0
14	0.0	0.0	0.0	8.37102758850013	0.0
15	0.0	0.0	0.0	8.643904086813784	0.0
16	0.0	0.0	0.0	9.26618917677858	0.0
17	0.0	0.0	0.0	9.98664646923146	0.0
18	0.0	0.0	0.0	10.83852783920871	0.0
19	0.0	0.0	0.0	11.276080290083025	0.0
20	0.0	0.0	0.0	11.7980819473987	0.0
21	0.0	0.0	0.0	12.396087890511604	0.0
22	0.0	0.0	0.0	13.05373608567372	0.0
23	0.0	0.0	0.0	13.689744171685236	0.0
24	0.0	0.0	0.0	14.214384866702206	0.0
25	0.0	0.0	0.0	14.62976940088566	0.0
26	0.0	0.0	0.0	15.030375323941877	0.0
27	0.0	0.0	0.0	15.437314937481197	0.0
28	0.0	0.0	0.0	15.863255622469822	0.0
29	0.0	0.0	0.0	16.283918232055864	0.0
30	0.0	0.0	0.0	16.73888833175871	0.0
31	0.0	0.0	0.0	17.20071992948491	0.0
32	5.278075402585201E-4	0.0	0.0	17.618215693829402	0.0
33	5.278075402585201E-4	0.0	0.0	18.03676707325441	0.0
34	5.278075402585201E-4	0.0	0.0	18.459013105461224	0.0
35	5.278075402585201E-4	0.0	0.0	18.8817869452083	0.0
36	5.278075402585201E-4	0.0	0.0	19.296115864311236	0.0
37	5.278075402585201E-4	0.0	0.0	19.71941751159857	0.0
38	5.278075402585201E-4	0.0	0.0	20.156969962472886	0.0
39	5.278075402585201E-4	0.0	0.0	20.581855032380993	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCACGA	40	6.7630026E-9	45.0	24
CGACGGT	40	6.7630026E-9	45.0	28
TAGCACG	25	3.8784397E-5	45.0	1
CGCAGGA	20	7.0183614E-4	45.0	4
CTATTGG	20	7.0183614E-4	45.0	2
CGGTCTA	40	6.7630026E-9	45.0	31
GCTACGG	20	7.0183614E-4	45.0	2
GTAAGGC	20	7.0183614E-4	45.0	4
TAGGAAC	20	7.0183614E-4	45.0	6
ACGGTCT	40	6.7630026E-9	45.0	30
GTTAGGA	55	6.002665E-11	40.909092	4
GTAGGGT	50	1.0713848E-9	40.5	4
TACGGGC	50	1.0713848E-9	40.5	4
TCGGGAA	45	1.9137588E-8	40.0	5
ACGGGAC	45	1.9137588E-8	40.0	5
CGGGCGA	45	1.9137588E-8	40.0	6
AACGGGA	85	0.0	39.705883	4
CGGGATA	40	3.4378718E-7	39.375	6
TACGGGA	75	0.0	39.0	4
ACGCCGG	35	6.2207055E-6	38.571426	27
>>END_MODULE