##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551172_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	105303
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.99542273249575	33.0	31.0	34.0	30.0	34.0
2	32.127071403473785	33.0	31.0	34.0	30.0	34.0
3	32.00745467840422	33.0	31.0	34.0	30.0	34.0
4	35.77826842540099	37.0	35.0	37.0	35.0	37.0
5	35.85255880649174	37.0	35.0	37.0	35.0	37.0
6	35.83609203916318	37.0	35.0	37.0	35.0	37.0
7	26.8618557875844	36.0	10.0	37.0	0.0	37.0
8	31.201067395990616	36.0	19.0	37.0	17.0	37.0
9	36.0026305043541	39.0	32.0	39.0	32.0	39.0
10	36.712866679961635	38.0	35.0	39.0	33.0	39.0
11	37.165512853384996	39.0	37.0	39.0	34.0	39.0
12	37.15901731194743	39.0	37.0	39.0	34.0	39.0
13	37.03345583696571	39.0	37.0	39.0	33.0	39.0
14	38.18447717538911	40.0	37.0	41.0	33.0	41.0
15	38.29629735145247	40.0	37.0	41.0	34.0	41.0
16	38.245130718023226	40.0	37.0	41.0	34.0	41.0
17	38.16628206223944	40.0	37.0	41.0	34.0	41.0
18	38.09370103415857	40.0	37.0	41.0	33.0	41.0
19	37.994321149444936	40.0	37.0	41.0	34.0	41.0
20	38.05759570002754	40.0	37.0	41.0	34.0	41.0
21	37.97714215169558	40.0	37.0	41.0	33.0	41.0
22	37.96493927048612	40.0	37.0	41.0	33.0	41.0
23	37.89779968282005	40.0	36.0	41.0	33.0	41.0
24	37.86104859310751	40.0	36.0	41.0	33.0	41.0
25	37.78677720482797	40.0	36.0	41.0	33.0	41.0
26	37.651235007549644	40.0	36.0	41.0	33.0	41.0
27	37.53360303125267	40.0	36.0	41.0	33.0	41.0
28	37.528455979411795	40.0	36.0	41.0	33.0	41.0
29	37.57483642441336	40.0	36.0	41.0	33.0	41.0
30	37.469768192739046	40.0	36.0	41.0	33.0	41.0
31	37.30468267760653	40.0	36.0	41.0	32.0	41.0
32	37.2131943059552	39.0	35.0	41.0	32.0	41.0
33	37.13539975119417	39.0	35.0	41.0	32.0	41.0
34	36.969943876242844	39.0	35.0	41.0	31.0	41.0
35	36.93259451297684	39.0	35.0	41.0	31.0	41.0
36	36.78563763615472	39.0	35.0	41.0	31.0	41.0
37	36.664586953837976	39.0	35.0	41.0	30.0	41.0
38	36.56591929954512	39.0	35.0	41.0	30.0	41.0
39	36.58820736351291	39.0	35.0	41.0	30.0	41.0
40	36.38435752067842	39.0	35.0	41.0	30.0	41.0
41	36.360872909603714	39.0	35.0	40.0	30.0	41.0
42	36.29209044376704	39.0	35.0	40.0	30.0	41.0
43	36.24604237296183	39.0	35.0	40.0	30.0	41.0
44	36.02772950438259	39.0	35.0	40.0	29.0	41.0
45	35.94278415619688	38.0	35.0	40.0	29.0	41.0
46	35.84620571113833	38.0	35.0	40.0	28.0	41.0
47	35.71198351423986	38.0	35.0	40.0	28.0	41.0
48	35.65551788647997	38.0	35.0	40.0	28.0	41.0
49	35.464193802645696	38.0	34.0	40.0	27.0	41.0
50	35.22065848076503	38.0	34.0	40.0	26.0	41.0
51	33.0866547011956	36.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	7.0
10	15.0
11	4.0
12	3.0
13	2.0
14	2.0
15	7.0
16	10.0
17	22.0
18	44.0
19	63.0
20	101.0
21	164.0
22	241.0
23	442.0
24	537.0
25	739.0
26	983.0
27	1107.0
28	1338.0
29	1547.0
30	1963.0
31	2580.0
32	3176.0
33	4536.0
34	7263.0
35	9369.0
36	9647.0
37	15771.0
38	20572.0
39	23041.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.76524885330902	21.534999002877413	27.85865549889367	10.841096644919897
2	30.443577106065355	25.505446188617608	26.866281112598884	17.184695592718153
3	31.482483879851475	24.64127327806425	27.973562006780433	15.902680835303837
4	29.224238625680183	25.68112969241142	26.9602955281426	18.1343361537658
5	26.644065221313735	30.626857734347553	23.690683076455564	19.038393967883156
6	26.26136007521153	36.1271758639355	23.577675849690895	14.033788211162076
7	62.09414736522226	28.03243972156539	6.023570078725203	3.8498428344871467
8	84.01565007644606	5.424346884704139	6.752894029609793	3.8071090092400026
9	76.85630988670789	9.712923658395297	8.284664254579642	5.14610220031718
10	36.098686647104074	38.18124839748155	14.193327825418079	11.526737129996297
11	32.851865568882175	25.865359961254665	23.552035554542606	17.73073891532055
12	32.08740491723883	22.95091307939945	27.09704376893346	17.864638234428266
13	25.264237486111508	27.104640893421838	27.88239651291986	19.748725107546793
14	19.604379742267554	31.058944189624228	27.85865549889367	21.47802056921455
15	19.164696162502494	27.29741792731451	34.87649924503575	18.66138666514724
16	22.492236688413435	25.317417357530175	32.54987987046903	19.640466083587363
17	22.049704186965233	25.565273543963613	28.12740377767015	24.257618491401004
18	23.1712296895625	26.353475209633153	28.853878806871595	21.621416293932747
19	24.899575510669212	26.937504154677455	27.367691328832038	20.795229005821298
20	26.7371299962964	26.617475285604396	27.305015051802894	19.340379666296307
21	25.15502882159103	26.624122769531734	27.816871314207575	20.403977094669667
22	22.991747623524496	26.175892424717244	27.014425040122315	23.817934911635945
23	22.739143234285823	26.60607959887183	28.3296772171733	22.32509994966905
24	22.342193479767907	25.74665489112371	30.115951112503918	21.795200516604467
25	20.98040891522559	27.792180659620335	28.61456938548759	22.61284103966649
26	20.744898056085773	29.914627313561816	26.84728830137793	22.49318632897448
27	22.027862454061136	29.202396892776083	27.353446720416326	21.416293932746456
28	18.378393777955043	29.39327464554666	30.838627579461175	21.38970399703712
29	21.10101326647864	26.034395981121143	29.70760567125343	23.156985081146786
30	22.09813585557866	27.41897191912861	28.510108923772354	21.972783301520373
31	22.48653884504715	29.62593658300333	25.365849026143607	22.521675545805913
32	24.64127327806425	27.456957541570514	26.38386370758668	21.517905472778555
33	23.360208161210984	28.165389400112055	26.38196442646458	22.09243801221238
34	20.922480841001683	28.238511723312726	27.964065601169956	22.874941834515635
35	21.28714281644398	26.285101089237724	27.997303020806623	24.430453073511675
36	23.8169852710749	27.946022430510055	26.961245168703645	21.2757471297114
37	22.011718564523328	30.124497877553345	28.292641235292443	19.571142322630884
38	20.965214666248823	29.174857316505705	28.07707282793463	21.782855189310844
39	22.202596317293903	26.696295452171352	28.432238397766447	22.668869832768294
40	22.648927380986297	26.43894286012744	28.98302992317408	21.929099835712183
41	20.36409219110567	27.323058222462798	28.157792275623677	24.15505731080786
42	21.843632185217896	25.707719628120756	27.658281340512612	24.790366846148732
43	22.323200668546956	26.235719780063242	28.024842597077004	23.41623695431279
44	20.825617503774822	27.9469720710711	27.445561854837948	23.781848570316136
45	20.243487839852616	27.31831001965756	26.998281150584504	25.439920989905325
46	21.007948491495966	29.05425296525265	27.231892728602226	22.705905814649157
47	21.121905358821685	27.74469863156795	29.2365839529738	21.896812056636563
48	21.13235140499321	25.68112969241142	29.32964872795647	23.8568701746389
49	21.24535863175788	25.188266241227698	29.55661282204686	24.00976230496757
50	19.4837753910145	26.451288187421063	29.915576954122862	24.149359467441574
51	19.704092001177553	26.303144259897625	27.702914446881856	26.28984929204296
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	61.0
1	129.0
2	197.0
3	367.5
4	538.0
5	389.5
6	241.0
7	233.5
8	226.0
9	225.0
10	224.0
11	219.0
12	214.0
13	222.5
14	231.0
15	230.5
16	230.0
17	214.5
18	199.0
19	227.0
20	255.0
21	301.0
22	347.0
23	408.5
24	470.0
25	598.0
26	999.0
27	1272.0
28	1529.0
29	1786.0
30	1925.5
31	2065.0
32	2398.5
33	2732.0
34	2975.5
35	3219.0
36	3530.5
37	3842.0
38	4239.0
39	4636.0
40	5267.0
41	5898.0
42	6580.5
43	7263.0
44	7818.0
45	8373.0
46	8902.5
47	9432.0
48	9540.0
49	9648.0
50	8948.5
51	8249.0
52	7470.5
53	6692.0
54	6102.5
55	5513.0
56	4996.0
57	4479.0
58	4335.0
59	4191.0
60	4004.5
61	3818.0
62	3413.5
63	3009.0
64	2420.0
65	1831.0
66	1481.5
67	1132.0
68	967.0
69	802.0
70	664.5
71	527.0
72	429.5
73	332.0
74	267.5
75	142.0
76	81.0
77	67.5
78	54.0
79	43.5
80	33.0
81	23.0
82	13.0
83	10.0
84	7.0
85	8.0
86	9.0
87	5.5
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	105303.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.78217144810689
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.52292055590127	31.829102684633863
2	9.95436631404273	9.114650104935281
3	5.270690728064717	7.239109996866186
4	3.6880315287284797	6.75384367017084
5	2.63638249325866	6.034965765457774
6	2.1178178801078618	5.817498076977864
7	1.528728479568554	4.899195654444792
8	1.1180253059531218	4.094850099237438
9	0.9479361128396597	3.9058716275889576
>10	3.177763949388094	18.779142094717148
>50	0.02696535988384153	0.8518275832597362
>100	0.01037129226301597	0.6799426417101126
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	221	0.2098705639915292	No Hit
GCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGC	152	0.1443453652792418	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	139	0.13200003798562243	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10446046171524079	0.0
2	0.0	0.0	0.0	0.5403454792361091	0.0
3	0.0	0.0	0.0	0.697985812370018	0.0
4	0.0	0.0	0.0	0.940144155437167	0.0
5	0.0	0.0	0.0	1.6504752951008044	0.0
6	0.0	0.0	0.0	1.9866480537116702	0.0
7	0.0	0.0	0.0	2.380748886546442	0.0
8	0.0	0.0	0.0	3.1471088193118906	0.0
9	0.0	0.0	0.0	3.450044158286089	0.0
10	0.0	0.0	0.0	4.301871741545825	0.0
11	0.0	0.0	0.0	5.2211238046399435	0.0
12	0.0	0.0	0.0	6.023570078725203	0.0
13	0.0	0.0	0.0	6.337901104431973	0.0
14	0.0	0.0	0.0	6.452807612318737	0.0
15	0.0	0.0	0.0	6.673124222481791	0.0
16	0.0	0.0	0.0	7.190678328252757	0.0
17	0.0	0.0	0.0	7.831685706959916	0.0
18	0.0	0.0	0.0	8.620837013190508	0.0
19	0.0	0.0	0.0	8.989297550876993	0.0
20	0.0	0.0	0.0	9.42613220895891	0.0
21	0.0	0.0	0.0	9.927542425192065	0.0
22	0.0	0.0	0.0	10.526765619213128	0.0
23	0.0	0.0	0.0	10.990190213004379	0.0
24	0.0	0.0	0.0	11.36434859405715	0.0
25	0.0	0.0	0.0	11.748953021281444	0.0
26	0.0	0.0	0.0	12.080377577087072	0.0
27	0.0	0.0	0.0	12.426046741308415	0.0
28	0.0	0.0	0.0	12.785010873384424	0.0
29	0.0	0.0	0.0	13.12973039704472	0.0
30	0.0	0.0	0.0	13.569413976809777	0.0
31	0.0	0.0	0.0	14.004349353769598	0.0
32	0.0	0.0	0.0	14.405097670531704	0.0
33	0.0	0.0	0.0	14.746968272508855	0.0
34	0.0	0.0	0.0	15.178105087224486	0.0
35	0.0	0.0	0.0	15.561759873887734	0.0
36	0.0	0.0	0.0	15.972954236821364	0.0
37	0.0	0.0	0.0	16.34141477450785	0.0
38	0.0	0.0	0.0	16.762105543051955	0.0
39	0.0	0.0	0.0	17.200839482255965	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGGG	20	7.004099E-4	45.000004	3
CCCTCGT	20	7.004099E-4	45.000004	14
CATGCAT	40	6.71389E-9	45.000004	25
TTCAATC	20	7.004099E-4	45.000004	30
ATATGGG	45	3.783498E-10	45.000004	3
GCTTGAG	20	7.004099E-4	45.000004	1
ACGGGAC	45	3.783498E-10	45.000004	5
TCGTGTA	20	7.004099E-4	45.000004	17
ATATCAC	40	6.71389E-9	45.000004	30
CCTCCGC	35	1.1979319E-7	45.000004	45
CATAGGA	20	7.004099E-4	45.000004	4
GCCCTCG	20	7.004099E-4	45.000004	13
GCCTCCG	35	1.1979319E-7	45.000004	44
ATAAGGA	20	7.004099E-4	45.000004	4
TTAGTGA	20	7.004099E-4	45.000004	25
TCTTTAG	20	7.004099E-4	45.000004	22
ATTTGCG	20	7.004099E-4	45.000004	1
CGCTTTC	20	7.004099E-4	45.000004	45
GAACGGG	25	3.866633E-5	45.0	3
GCGATAG	25	3.866633E-5	45.0	9
>>END_MODULE