SRR3551171_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3551171_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	230312
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	552	0.23967487582062594	RNA PCR Primer, Index 35 (95% over 23bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCG	476	0.20667616103372816	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTC	474	0.205807773802494	RNA PCR Primer, Index 10 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	448	0.19451873979645	RNA PCR Primer, Index 10 (95% over 24bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGC	374	0.16238841224078643	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	343	0.14892841015665706	No Hit
CTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGCT	315	0.13677098891937892	TruSeq Adapter, Index 10 (96% over 25bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	293	0.12721872937580325	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCGAC	20	7.0215284E-4	45.000004	9
AGTATAC	20	7.0215284E-4	45.000004	19
CGCTAGG	20	7.0215284E-4	45.000004	2
CTCGCCA	20	7.0215284E-4	45.000004	41
CACGTCC	20	7.0215284E-4	45.000004	37
TGTAACG	20	7.0215284E-4	45.000004	1
CTAGCGG	30	2.1579199E-6	45.000004	2
TACGCGG	35	1.206281E-7	45.0	2
GCGGGCT	25	3.8810635E-5	45.0	5
TTTGCGG	35	1.206281E-7	45.0	2
AGGCGAT	75	0.0	42.0	7
TCACGAC	65	0.0	41.538464	25
CGGTCTA	65	0.0	41.538464	31
ACGGTCT	65	0.0	41.538464	30
TACGGCT	60	3.6379788E-12	41.250004	7
GGCATAG	50	1.0732037E-9	40.5	1
TGCGAGG	40	3.4421828E-7	39.375004	2
AAGGCGA	40	3.4421828E-7	39.375004	6
GGCGATT	115	0.0	39.130436	8
GGTGTGC	35	6.226559E-6	38.571426	8