##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551171_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	230312
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.903161797908922	31.0	31.0	34.0	30.0	34.0
2	32.02645107506339	33.0	31.0	34.0	30.0	34.0
3	31.955395289867656	33.0	31.0	34.0	30.0	34.0
4	35.70849977421932	37.0	35.0	37.0	35.0	37.0
5	35.78773576713328	37.0	35.0	37.0	35.0	37.0
6	35.76915662232103	37.0	35.0	37.0	35.0	37.0
7	26.955638438292404	35.0	15.0	37.0	0.0	37.0
8	31.22227673764285	35.0	21.0	37.0	17.0	37.0
9	35.94830490812463	39.0	32.0	39.0	32.0	39.0
10	36.6049489388308	38.0	35.0	39.0	32.0	39.0
11	37.04710132342214	39.0	37.0	39.0	34.0	39.0
12	37.16435965125569	39.0	37.0	39.0	34.0	39.0
13	37.13658428566466	39.0	37.0	39.0	34.0	39.0
14	38.28047170794401	40.0	38.0	41.0	34.0	41.0
15	38.35845722324499	40.0	38.0	41.0	34.0	41.0
16	38.28206519851332	40.0	37.0	41.0	34.0	41.0
17	38.185587377123205	40.0	37.0	41.0	33.0	41.0
18	38.10517906144708	40.0	37.0	41.0	33.0	41.0
19	38.04883375594845	40.0	37.0	41.0	33.0	41.0
20	38.10772777797075	40.0	37.0	41.0	34.0	41.0
21	38.0487599430338	40.0	37.0	41.0	33.0	41.0
22	38.06321424849769	40.0	37.0	41.0	34.0	41.0
23	37.9969389350099	40.0	37.0	41.0	33.0	41.0
24	37.961404529507796	40.0	37.0	41.0	33.0	41.0
25	37.87027163152593	40.0	37.0	41.0	33.0	41.0
26	37.74046510820105	40.0	37.0	41.0	33.0	41.0
27	37.65292299142033	40.0	36.0	41.0	33.0	41.0
28	37.59432856299281	40.0	36.0	41.0	33.0	41.0
29	37.59214456910625	40.0	36.0	41.0	33.0	41.0
30	37.52348553266873	40.0	36.0	41.0	33.0	41.0
31	37.36226075931779	39.0	36.0	41.0	32.0	41.0
32	37.24231477300358	39.0	36.0	41.0	31.0	41.0
33	37.18536159644309	39.0	36.0	41.0	31.0	41.0
34	37.08752040709994	39.0	36.0	41.0	31.0	41.0
35	37.01469745388864	39.0	36.0	41.0	31.0	41.0
36	36.881499878425785	39.0	35.0	41.0	31.0	41.0
37	36.77028552572163	39.0	35.0	41.0	30.0	41.0
38	36.658168050296986	39.0	35.0	41.0	30.0	41.0
39	36.57574073430824	39.0	35.0	41.0	30.0	41.0
40	36.4218972524228	39.0	35.0	41.0	30.0	41.0
41	36.333256625794576	39.0	35.0	40.0	30.0	41.0
42	36.27549150717288	39.0	35.0	40.0	30.0	41.0
43	36.20881673555872	39.0	35.0	40.0	29.0	41.0
44	35.96232067803675	39.0	35.0	40.0	28.0	41.0
45	35.903101010802736	39.0	35.0	40.0	28.0	41.0
46	35.72683142867067	38.0	35.0	40.0	27.0	41.0
47	35.63841224078641	38.0	35.0	40.0	27.0	41.0
48	35.538408767237485	38.0	35.0	40.0	26.0	41.0
49	35.3575844940776	38.0	34.0	40.0	26.0	41.0
50	35.07162023689604	38.0	34.0	40.0	24.0	41.0
51	32.70218225711209	36.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	16.0
10	13.0
11	6.0
12	3.0
13	6.0
14	5.0
15	4.0
16	12.0
17	20.0
18	56.0
19	102.0
20	177.0
21	338.0
22	539.0
23	817.0
24	1214.0
25	1671.0
26	2021.0
27	2657.0
28	3191.0
29	3728.0
30	4708.0
31	5807.0
32	7505.0
33	9835.0
34	14448.0
35	18584.0
36	22094.0
37	34605.0
38	45671.0
39	50445.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.545920316787665	22.00666921393588	26.962555142589185	11.484855326687276
2	28.34980374448574	26.694657681753448	27.898242384243982	17.05729618951683
3	30.364462120948975	25.015630970162217	28.44619472715273	16.17371218173608
4	28.330265031782975	26.592622182083435	27.69590815936642	17.38120462676717
5	24.89883288756122	30.556809892667335	24.969172253291188	19.57518496648025
6	25.23055680989267	35.929955885928656	24.54713605891139	14.292351245267291
7	61.999374761193515	27.330317135016845	6.3978429261176135	4.272465177672028
8	82.71952829205598	5.6471221647156895	6.86633783736844	4.767011705859877
9	75.472836847407	9.220969814859842	9.279585952968148	6.026607384765014
10	30.966688665809862	41.58055159956928	15.137726214873737	12.315033519747127
11	28.2013095279447	25.62176525756365	27.617753308555347	18.559171905936296
12	27.62817395533016	23.030497759560944	29.53384973427351	19.807478550835388
13	24.127270832609675	26.039893709402897	31.123866754663236	18.708968703324185
14	19.751467574420786	28.561690228906873	29.01629094445795	22.670551252214388
15	18.883514536802252	26.945621591580114	33.76332974399944	20.407534127618188
16	21.861648546319774	27.820955920664144	29.718382020910767	20.59901351210532
17	20.98284066831081	27.34030358817604	28.23995275973462	23.436902983778527
18	21.41963944562159	26.72374865399979	31.28712355413526	20.569488346243357
19	22.05964083504116	26.805377053735803	28.4783250547084	22.65665705651464
20	21.827781444301646	28.306818576539648	29.651082010490114	20.21431796866859
21	22.48341380388343	27.87783528430998	29.635885233943522	20.00286567786307
22	21.52949043037271	24.99218451491889	28.95420125742471	24.524123797283686
23	19.566066900552297	26.776720275105077	30.362725346486506	23.294487477856126
24	20.45746639341415	25.94263433950467	30.590676994685474	23.009222272395707
25	18.876567438952378	29.344541317864465	28.49265344402376	23.2862377991594
26	19.1644378061065	28.559953454444404	28.30985793184897	23.965750807600124
27	20.734916113793464	26.948660946889436	29.621556844628156	22.694866094688944
28	17.751571780888533	26.939977074577094	31.264545486123176	24.043905658411198
29	18.917381638820384	25.151099378234743	30.431327937754006	25.500191045190874
30	20.007207614019244	26.019486609468895	31.197245475702523	22.776060300809338
31	20.688457396922434	26.160165340928828	27.811837854736183	25.339539407412552
32	20.146583764632325	26.084181458195836	30.15083886206537	23.618395915106465
33	19.426256556323594	25.223175518427176	30.297162110528326	25.053405814720904
34	17.814529855153012	24.64048768626906	31.0491854527771	26.495797005800824
35	18.624735141894476	23.545017194067178	32.08300045156136	25.747247212476985
36	19.696759178853036	24.378234742436348	30.74003959845775	25.184966480252875
37	17.88877696342353	25.844940775990832	32.71431796866859	23.551964291917052
38	17.851002118864844	26.84705964083504	30.943676404182153	24.35826183611796
39	19.411928167008234	23.869794018548752	31.610163604154362	25.10811421028865
40	20.290736045017194	23.75429851679461	32.060422383549266	23.894543054638927
41	17.795425336065858	24.761627705026225	29.408367779360173	28.03457917954774
42	18.698548056549377	24.15505922400917	31.135589982284902	26.010802737156553
43	20.622894160964258	23.442113307165933	30.640609260481433	25.294383271388376
44	19.055021014970997	25.028656778630726	30.131734342978223	25.784587863420054
45	18.90435583035187	24.21020181319254	30.28978081906284	26.595661537392758
46	20.424467678627252	24.778127062419674	30.329726631699604	24.467678627253463
47	17.69599499808955	23.73172044878252	33.18845739692244	25.383827156205495
48	18.507068672062246	23.82984820591198	31.805984924797663	25.857098197228108
49	19.311629441800687	22.500347354892494	32.865851540518946	25.322171662787866
50	18.12932022647539	22.817308694292958	32.92359929139602	26.129771787835633
51	18.93127583452013	21.78870401889611	30.313227274306158	28.96679287227761
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	111.0
1	201.5
2	292.0
3	549.5
4	807.0
5	589.5
6	372.0
7	366.0
8	360.0
9	346.5
10	333.0
11	344.5
12	356.0
13	352.5
14	349.0
15	368.5
16	388.0
17	410.5
18	433.0
19	503.0
20	573.0
21	670.5
22	768.0
23	847.0
24	926.0
25	1309.5
26	2190.5
27	2688.0
28	3250.5
29	3813.0
30	4250.5
31	4688.0
32	5383.0
33	6078.0
34	7100.0
35	8122.0
36	8989.5
37	9857.0
38	10471.0
39	11085.0
40	12386.5
41	13688.0
42	14433.5
43	15179.0
44	17459.5
45	19740.0
46	21992.5
47	24245.0
48	24240.0
49	24235.0
50	22636.0
51	21037.0
52	17844.5
53	14652.0
54	12605.5
55	10559.0
56	9589.0
57	8619.0
58	7786.0
59	6953.0
60	6249.5
61	5546.0
62	4682.0
63	3818.0
64	3377.5
65	2937.0
66	2433.5
67	1930.0
68	1583.5
69	1237.0
70	1009.0
71	781.0
72	611.0
73	441.0
74	335.5
75	194.0
76	158.0
77	126.5
78	95.0
79	73.5
80	52.0
81	47.0
82	42.0
83	38.0
84	34.0
85	20.5
86	7.0
87	4.5
88	2.0
89	2.5
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	230312.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.826950571666416
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.28690448812651	31.50747593324685
2	10.48052114979626	9.396196070944427
3	4.96932172543425	6.682786180822475
4	3.1921175204306547	5.723715772291812
5	2.275780021338649	5.100813926426678
6	1.766264938089632	4.750576264572683
7	1.3796438350648528	4.329165820066951
8	1.0859324814784153	3.894323333711998
9	0.916099429395594	3.6959349456223283
>10	3.604456640463253	22.13799200208823
>50	0.02797250164403238	0.8795366810634037
>100	0.01398625082201619	1.6542813135683363
>500	9.990179158582994E-4	0.2472017555738283
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	552	0.23967487582062594	RNA PCR Primer, Index 35 (95% over 23bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCG	476	0.20667616103372816	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTC	474	0.205807773802494	RNA PCR Primer, Index 10 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	448	0.19451873979645	RNA PCR Primer, Index 10 (95% over 24bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGC	374	0.16238841224078643	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	343	0.14892841015665706	No Hit
CTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGCT	315	0.13677098891937892	TruSeq Adapter, Index 10 (96% over 25bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	293	0.12721872937580325	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.3419361561707596E-4	0.0	0.0	0.2131890652679843	0.0
2	4.3419361561707596E-4	0.0	0.0	1.1454027579978463	0.0
3	4.3419361561707596E-4	0.0	0.0	1.468877001632568	0.0
4	4.3419361561707596E-4	0.0	0.0	1.8904790023967488	0.0
5	4.3419361561707596E-4	0.0	0.0	3.2264927576504916	0.0
6	4.3419361561707596E-4	0.0	0.0	3.850863176907847	0.0
7	4.3419361561707596E-4	0.0	0.0	4.548612317204488	0.0
8	4.3419361561707596E-4	0.0	0.0	6.033554482614887	0.0
9	4.3419361561707596E-4	0.0	0.0	6.536784883115079	0.0
10	4.3419361561707596E-4	0.0	0.0	7.959637361492237	0.0
11	4.3419361561707596E-4	0.0	0.0	9.314321442217514	0.0
12	4.3419361561707596E-4	0.0	0.0	10.66900552294279	0.0
13	4.3419361561707596E-4	0.0	0.0	11.14444753204349	0.0
14	4.3419361561707596E-4	0.0	0.0	11.311612074056063	0.0
15	4.3419361561707596E-4	0.0	0.0	11.681545034561811	0.0
16	4.3419361561707596E-4	0.0	0.0	12.399701274792456	0.0
17	4.3419361561707596E-4	0.0	0.0	13.312810448435167	0.0
18	4.3419361561707596E-4	0.0	0.0	14.34402028552572	0.0
19	4.3419361561707596E-4	0.0	0.0	14.885459724200215	0.0
20	4.3419361561707596E-4	0.0	0.0	15.50244885199208	0.0
21	4.3419361561707596E-4	0.0	0.0	16.279655423946647	0.0
22	4.3419361561707596E-4	0.0	0.0	17.084650387300705	0.0
23	4.3419361561707596E-4	0.0	0.0	17.855344055021014	0.0
24	4.3419361561707596E-4	0.0	0.0	18.43195317656049	0.0
25	4.3419361561707596E-4	0.0	0.0	18.9534197089166	0.0
26	4.3419361561707596E-4	0.0	0.0	19.44753204348883	0.0
27	4.3419361561707596E-4	0.0	0.0	19.922105665358295	0.0
28	4.3419361561707596E-4	0.0	0.0	20.430546389245894	0.0
29	4.3419361561707596E-4	0.0	0.0	20.94593421098336	0.0
30	4.3419361561707596E-4	0.0	0.0	21.56075237069714	0.0
31	4.3419361561707596E-4	0.0	0.0	22.084389871131336	0.0
32	4.3419361561707596E-4	0.0	0.0	22.585015109937824	0.0
33	4.3419361561707596E-4	0.0	0.0	23.083903574281845	0.0
34	4.3419361561707596E-4	0.0	0.0	23.55674042168884	0.0
35	4.3419361561707596E-4	0.0	0.0	24.10165340928827	0.0
36	4.3419361561707596E-4	0.0	0.0	24.595765743860504	0.0
37	4.3419361561707596E-4	0.0	0.0	25.043419361561707	0.0
38	4.3419361561707596E-4	0.0	0.0	25.530584598284065	0.0
39	4.3419361561707596E-4	0.0	0.0	26.032946611553022	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCGAC	20	7.0215284E-4	45.000004	9
AGTATAC	20	7.0215284E-4	45.000004	19
CGCTAGG	20	7.0215284E-4	45.000004	2
CTCGCCA	20	7.0215284E-4	45.000004	41
CACGTCC	20	7.0215284E-4	45.000004	37
TGTAACG	20	7.0215284E-4	45.000004	1
CTAGCGG	30	2.1579199E-6	45.000004	2
TACGCGG	35	1.206281E-7	45.0	2
GCGGGCT	25	3.8810635E-5	45.0	5
TTTGCGG	35	1.206281E-7	45.0	2
AGGCGAT	75	0.0	42.0	7
TCACGAC	65	0.0	41.538464	25
CGGTCTA	65	0.0	41.538464	31
ACGGTCT	65	0.0	41.538464	30
TACGGCT	60	3.6379788E-12	41.250004	7
GGCATAG	50	1.0732037E-9	40.5	1
TGCGAGG	40	3.4421828E-7	39.375004	2
AAGGCGA	40	3.4421828E-7	39.375004	6
GGCGATT	115	0.0	39.130436	8
GGTGTGC	35	6.226559E-6	38.571426	8
>>END_MODULE