FastQCFastQC Report
Sat 18 Jun 2016
SRR3551170_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551170_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences221294
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16010.7234719423029996No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2370.10709734561262392No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT2330.10528979547570201No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAT207.020927E-445.00000443
GTCTCGC207.020927E-445.00000431
TCTCGCG207.020927E-445.00000432
CCGAGGG351.20599E-745.0000043
AGTCTCG207.020927E-445.00000430
ATGCGCG207.020927E-445.0000041
AGTACGG207.020927E-445.0000042
CTCACGT850.045.045
CGACGGT950.045.028
CGTAAAG253.880565E-545.02
ACGGTCT950.045.030
CGTTTTT6450.042.558141
TACGAAT650.041.5384612
CGAATAT650.041.5384614
CGACCAC603.6379788E-1241.24999620
ATAGGAC556.002665E-1140.9090925
TAGGGTA1100.040.9090925
CGGTCTA1050.040.71428731
CGTTTTA1050.040.7142871
CAACGCA750.039.017