FastQCFastQC Report
Sat 18 Jun 2016
SRR3551169_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551169_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141084
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2590.1835785773014658No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT2490.17649060134388025No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCG1530.10844603215105894No Hit
GCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTGC1520.10773723455530038No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGC1490.10561084176802472No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGGG302.1519336E-645.0000043
GGCACGG207.0122356E-445.02
GACAACA207.0122356E-445.09
GCACGGG453.8016879E-1045.03
TTGAGCG207.0122356E-445.01
CTACGGG207.0122356E-445.03
GCGATGT406.7429937E-945.09
CACACCG207.0122356E-445.017
ATAGTTA207.0122356E-445.037
AGTACGG207.0122356E-445.02
CCCGATG207.0122356E-445.037
CGAAGGA253.8733677E-544.9999964
TGGGATC800.042.18756
ATAAGGA603.6379788E-1241.2500044
CGGGCGA556.002665E-1140.9090926
ATGCGGG556.002665E-1140.9090923
TTGGGAT1250.039.65
TAGGGTC403.4295408E-739.3755
GCTACGA800.039.37510
AGCTACG800.039.3759