##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551169_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	141084
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.05709364633835	33.0	31.0	34.0	30.0	34.0
2	32.19184315726801	33.0	31.0	34.0	30.0	34.0
3	32.09092455558391	33.0	31.0	34.0	30.0	34.0
4	35.81477701227637	37.0	35.0	37.0	35.0	37.0
5	35.89037027586402	37.0	35.0	37.0	35.0	37.0
6	35.858049105497436	37.0	35.0	37.0	35.0	37.0
7	26.745513311218847	36.0	10.0	37.0	0.0	37.0
8	31.14991777947889	36.0	19.0	37.0	17.0	37.0
9	35.99549913526693	39.0	32.0	39.0	32.0	39.0
10	36.721974143063704	38.0	35.0	39.0	33.0	39.0
11	37.18676816648238	39.0	37.0	39.0	34.0	39.0
12	37.23722037934847	39.0	37.0	39.0	34.0	39.0
13	37.186257832213435	39.0	37.0	39.0	34.0	39.0
14	38.40037140994018	40.0	38.0	41.0	34.0	41.0
15	38.49086359899067	40.0	38.0	41.0	34.0	41.0
16	38.44242437129653	40.0	38.0	41.0	34.0	41.0
17	38.35337104136543	40.0	38.0	41.0	34.0	41.0
18	38.26219131864705	40.0	37.0	41.0	34.0	41.0
19	38.16308723880809	40.0	37.0	41.0	34.0	41.0
20	38.21476567151484	40.0	37.0	41.0	34.0	41.0
21	38.15687817186924	40.0	37.0	41.0	34.0	41.0
22	38.14944997306569	40.0	37.0	41.0	34.0	41.0
23	38.09178928865073	40.0	37.0	41.0	34.0	41.0
24	38.06927078903348	40.0	37.0	41.0	34.0	41.0
25	37.98952397153469	40.0	37.0	41.0	33.0	41.0
26	37.86145842193303	40.0	37.0	41.0	33.0	41.0
27	37.745846446088855	40.0	36.0	41.0	33.0	41.0
28	37.70983952822432	40.0	36.0	41.0	33.0	41.0
29	37.72313657112075	40.0	36.0	41.0	33.0	41.0
30	37.6539933656545	40.0	36.0	41.0	33.0	41.0
31	37.55795837940518	40.0	36.0	41.0	33.0	41.0
32	37.44529500155935	40.0	36.0	41.0	33.0	41.0
33	37.34586487482635	40.0	36.0	41.0	32.0	41.0
34	37.24857531683252	40.0	36.0	41.0	32.0	41.0
35	37.13569221173202	40.0	36.0	41.0	31.0	41.0
36	37.007414022851634	40.0	35.0	41.0	31.0	41.0
37	36.93877406367837	39.0	35.0	41.0	31.0	41.0
38	36.85899180629979	39.0	35.0	41.0	31.0	41.0
39	36.85465396501375	39.0	35.0	41.0	31.0	41.0
40	36.66148535624167	39.0	35.0	41.0	30.0	41.0
41	36.58968415979133	39.0	35.0	41.0	30.0	41.0
42	36.52185222987723	39.0	35.0	41.0	30.0	41.0
43	36.48647614187293	39.0	35.0	41.0	30.0	41.0
44	36.299325224688836	39.0	35.0	40.0	30.0	41.0
45	36.21997533384367	39.0	35.0	40.0	29.0	41.0
46	36.09726120608999	39.0	35.0	40.0	29.0	41.0
47	35.98192566130815	39.0	35.0	40.0	28.0	41.0
48	35.92455558390746	39.0	35.0	40.0	28.0	41.0
49	35.79091179722718	38.0	35.0	40.0	28.0	41.0
50	35.56754841087579	38.0	35.0	40.0	27.0	41.0
51	33.56912194153838	36.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	2.0
8	2.0
9	12.0
10	14.0
11	8.0
12	0.0
13	6.0
14	2.0
15	10.0
16	7.0
17	23.0
18	43.0
19	68.0
20	120.0
21	234.0
22	303.0
23	479.0
24	640.0
25	916.0
26	1198.0
27	1431.0
28	1730.0
29	1930.0
30	2397.0
31	3082.0
32	3985.0
33	5693.0
34	8966.0
35	11295.0
36	13098.0
37	21312.0
38	28987.0
39	33082.0
40	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.835020271611235	22.563153865782088	26.903121544611718	11.698704317994954
2	29.07558617561169	25.885996994698196	27.87346545320518	17.16495137648493
3	32.824416659578695	25.25162314649429	26.53950837798758	15.38445181593944
4	28.456097076918713	26.483513367922658	26.636613648606506	18.423775906552127
5	26.121317796490036	31.242380425845596	24.29261999943296	18.343681778231407
6	27.25822914008676	36.45771313543704	22.60213773354881	13.68191999092739
7	62.79875818661223	28.32284312891611	5.221711887953276	3.656686796518386
8	84.84803379546936	5.590286637747725	6.273567520058972	3.288112046723937
9	77.03921068299736	10.055711491026623	8.177397862266451	4.727679963709563
10	34.29800686116073	40.33979756740665	13.53874287658416	11.82345269484846
11	32.08726715998979	26.414051203538317	24.561963085821212	16.936718550650674
12	31.107000085055713	23.22375322502906	26.21204388874713	19.4572028011681
13	25.551444529500156	25.542230160755295	28.140682146806157	20.765643162938392
14	20.719571319214086	28.563834311474018	27.419834991919707	23.296759377392192
15	19.692523602959938	27.64948541294548	31.988035496583596	20.669955487510986
16	23.71849394686853	26.3339570752176	30.154376116356215	19.793172861557654
17	23.08199370587735	26.443111904964418	27.314224150151684	23.16067023900655
18	23.481755549885175	26.487766153497205	28.785687958946443	21.244790337671173
19	24.291911201837205	28.391596495704686	26.583453828924615	20.733038473533497
20	26.48705735590145	26.79042272688611	27.725326755691643	18.997193161520794
21	25.346602024325932	27.58144084375266	27.164667857446627	19.90728927447478
22	23.62635025941992	25.783221343313205	27.399988659238467	23.19043973802841
23	22.764452382977517	27.8132176575657	27.91315811856766	21.509171840889117
24	22.623401661421564	26.11352102293669	28.990530464120667	22.27254685152108
25	21.662272121572965	27.867086274843356	27.27240509200193	23.19823651158175
26	22.011709336281932	29.38958351053273	26.92438547248448	21.67432168070086
27	21.896175328173285	29.344220464404184	27.939383629610724	20.8202205778118
28	19.19636528592895	29.257038360125883	29.755323069944144	21.79127328400102
29	22.16480961696578	27.37872473136571	28.811204672393753	21.64526097927476
30	22.284596410648973	28.253380964531765	27.955685974313177	21.506336650506082
31	22.769413966147827	28.929573870885427	26.23047262623685	22.070539536729893
32	24.111167814918772	29.281137478381673	26.26024212525871	20.347452581440844
33	22.960789317002636	29.61143715800516	25.72013835729069	21.70763516770151
34	21.798361259958607	27.808256074395395	27.38864789770633	23.004734767939667
35	21.89546653057753	27.632474270647272	26.98179807774092	23.49026112103428
36	22.030138073771653	29.867313090074	26.55226673471124	21.55028210144311
37	21.27668622948031	29.908423350627995	27.126392787275666	21.68849763261603
38	20.13835729069207	30.122480224547076	26.438859119389868	23.300303365370983
39	22.210172663094326	28.009554591590824	27.329108899662614	22.451163845652236
40	22.132913725156644	27.304300983811064	28.436250744237473	22.12653454679482
41	19.850585466814096	27.799750503246294	27.622551104306652	24.72711292563296
42	21.33055484675796	27.494967537070114	27.49284114428284	23.681636471889085
43	21.40923137988716	28.222193870318392	27.489297156304044	22.879277593490404
44	20.92724901477134	28.196677156871086	26.787587536503075	24.088486291854498
45	20.803918233109354	28.549658359558844	26.139037736383997	24.507385670947805
46	21.229905588160243	29.980011907799607	26.4211391794959	22.368943324544244
47	20.564344645742963	28.06413200646423	28.436959541833236	22.93456380595957
48	20.83368773213121	26.85846729607893	28.62408210711349	23.683762864676364
49	20.8698364095149	27.055513027699813	28.518471265345468	23.556179297439822
50	19.50610983527544	27.59986958124238	28.553911145133398	24.340109438348787
51	19.635110997703496	27.118596013722325	27.065436194040434	26.180856794533753
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	59.0
1	149.0
2	239.0
3	440.0
4	641.0
5	467.0
6	293.0
7	285.5
8	278.0
9	259.5
10	241.0
11	254.5
12	268.0
13	273.5
14	279.0
15	292.5
16	306.0
17	315.0
18	324.0
19	342.0
20	360.0
21	452.0
22	544.0
23	610.0
24	676.0
25	829.5
26	1336.5
27	1690.0
28	2049.0
29	2408.0
30	2804.0
31	3200.0
32	3601.0
33	4002.0
34	4655.0
35	5308.0
36	5507.0
37	5706.0
38	6258.5
39	6811.0
40	7404.5
41	7998.0
42	8576.5
43	9155.0
44	9757.5
45	10360.0
46	10944.0
47	11528.0
48	11508.0
49	11488.0
50	11077.0
51	10666.0
52	9659.0
53	8652.0
54	7920.5
55	7189.0
56	6775.5
57	6362.0
58	6289.5
59	6217.0
60	5873.0
61	5529.0
62	4742.0
63	3955.0
64	3406.5
65	2858.0
66	2295.0
67	1732.0
68	1411.5
69	1091.0
70	857.5
71	624.0
72	518.5
73	413.0
74	356.5
75	242.0
76	184.0
77	126.0
78	68.0
79	56.0
80	44.0
81	33.5
82	23.0
83	18.0
84	13.0
85	11.5
86	10.0
87	7.5
88	5.0
89	4.0
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	141084.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.60485951631652
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.96903516242851	31.676873352045593
2	10.769254167173623	10.03799155113266
3	6.057610414892323	8.469422471718977
4	4.028774790120453	7.510419324657651
5	2.772539238350164	6.460690085339231
6	2.047086020197104	5.724249383346091
7	1.5771383379973234	5.145161747611352
8	1.2896946100498845	4.808482889626038
9	1.0174595449568073	4.26767032406226
>10	2.4531573184085653	14.755039550905844
>50	0.007604331427180922	0.27855745513311214
>100	0.010646063998053292	0.8654418644211959
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	259	0.1835785773014658	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	249	0.17649060134388025	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCG	153	0.10844603215105894	No Hit
GCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTGC	152	0.10773723455530038	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGC	149	0.10561084176802472	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07796773553344107	0.0
2	0.0	0.0	0.0	0.3877122848799297	0.0
3	0.0	0.0	0.0	0.4833999603073346	0.0
4	0.0	0.0	0.0	0.6450058121402852	0.0
5	0.0	0.0	0.0	1.12344418927731	0.0
6	0.0	0.0	0.0	1.3715233477928044	0.0
7	0.0	0.0	0.0	1.6670919452241217	0.0
8	0.0	0.0	0.0	2.3943182784723995	0.0
9	0.0	0.0	0.0	2.660826174477616	0.0
10	0.0	0.0	0.0	3.3837997221513425	0.0
11	0.0	0.0	0.0	4.160641887102719	0.0
12	0.0	0.0	0.0	4.931813671288027	0.0
13	0.0	0.0	0.0	5.213206316804174	0.0
14	0.0	0.0	0.0	5.3181083609764395	0.0
15	0.0	0.0	0.0	5.541379603640385	0.0
16	0.0	0.0	0.0	5.953191006776105	0.0
17	0.0	0.0	0.0	6.4500581214028525	0.0
18	0.0	0.0	0.0	7.01071701964787	0.0
19	0.0	0.0	0.0	7.314791188228289	0.0
20	0.0	0.0	0.0	7.6741515692778774	0.0
21	0.0	0.0	0.0	8.102974114711802	0.0
22	0.0	0.0	0.0	8.582830087040344	0.0
23	0.0	0.0	0.0	9.023702191602165	0.0
24	0.0	0.0	0.0	9.392985738992373	0.0
25	0.0	0.0	0.0	9.715488645062516	0.0
26	0.0	0.0	0.0	10.001134076153214	0.0
27	0.0	0.0	0.0	10.29741147118029	0.0
28	0.0	0.0	0.0	10.587309687845538	0.0
29	0.0	0.0	0.0	10.933911712171472	0.0
30	0.0	0.0	0.0	11.303904057157439	0.0
31	0.0014175951915171103	0.0	0.0	11.64625329590882	0.0
32	0.0014175951915171103	0.0	0.0	11.962377023617137	0.0
33	0.0014175951915171103	0.0	0.0	12.287006322474554	0.0
34	0.0014175951915171103	0.0	0.0	12.630064358821695	0.0
35	0.0014175951915171103	0.0	0.0	12.996512715828867	0.0
36	0.0014175951915171103	0.0	0.0	13.359417084857249	0.0
37	0.0014175951915171103	0.0	0.0	13.721612656289869	0.0
38	0.0014175951915171103	0.0	0.0	14.08451702531825	0.0
39	0.0014175951915171103	0.0	0.0	14.42261347849508	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGGG	30	2.1519336E-6	45.000004	3
GGCACGG	20	7.0122356E-4	45.0	2
GACAACA	20	7.0122356E-4	45.0	9
GCACGGG	45	3.8016879E-10	45.0	3
TTGAGCG	20	7.0122356E-4	45.0	1
CTACGGG	20	7.0122356E-4	45.0	3
GCGATGT	40	6.7429937E-9	45.0	9
CACACCG	20	7.0122356E-4	45.0	17
ATAGTTA	20	7.0122356E-4	45.0	37
AGTACGG	20	7.0122356E-4	45.0	2
CCCGATG	20	7.0122356E-4	45.0	37
CGAAGGA	25	3.8733677E-5	44.999996	4
TGGGATC	80	0.0	42.1875	6
ATAAGGA	60	3.6379788E-12	41.250004	4
CGGGCGA	55	6.002665E-11	40.909092	6
ATGCGGG	55	6.002665E-11	40.909092	3
TTGGGAT	125	0.0	39.6	5
TAGGGTC	40	3.4295408E-7	39.375	5
GCTACGA	80	0.0	39.375	10
AGCTACG	80	0.0	39.375	9
>>END_MODULE