FastQCFastQC Report
Sat 18 Jun 2016
SRR3551168_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551168_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167818
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCG5410.3223730469913835No Hit
GAATCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTC5240.31224302518204244No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5000.29794181792179625No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGC4770.2842364942973936No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT2270.1352655853364955No Hit
GCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC1930.11500554171781335Illumina Single End Adapter 1 (95% over 21bp)
GAGGAGCTGAGAACCCCAGTCTAATAATGTCCATCGACACCTCCTTATCCC1800.10725905445184664No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCGG207.016057E-445.00000424
CACGCCG302.1543947E-645.00000426
CTACATT207.016057E-445.00000422
CGTAACC302.1543947E-645.00000435
CGGTCTA302.1543947E-645.00000431
TAGGAAC207.016057E-445.0000046
GCGAGAC551.8189894E-1245.021
ACCGGAG253.8765313E-545.032
CGACACC551.8189894E-1245.035
ACCTCCT502.1827873E-1145.039
CCATCGA551.8189894E-1245.031
TCGTCCC253.8765313E-545.038
CGAATAT351.2036435E-745.014
AACGACG253.8765313E-545.013
CACCGGA253.8765313E-545.031
GCTCGTA253.8765313E-545.032
TCGACAC551.8189894E-1245.034
TAGCTCG253.8765313E-545.030
ACACGAC603.6379788E-1241.25000426
GTAGGGT603.6379788E-1241.2500044