##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551168_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	167818
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.04394045930711	33.0	31.0	34.0	30.0	34.0
2	32.153511542266024	33.0	31.0	34.0	30.0	34.0
3	32.047575349485754	33.0	31.0	34.0	30.0	34.0
4	35.784957513496764	37.0	35.0	37.0	35.0	37.0
5	35.85945488564993	37.0	35.0	37.0	35.0	37.0
6	35.83899224159506	37.0	35.0	37.0	35.0	37.0
7	26.81119426998296	35.0	10.0	37.0	0.0	37.0
8	31.134312171519145	35.0	19.0	37.0	17.0	37.0
9	35.94375454361273	39.0	32.0	39.0	32.0	39.0
10	36.73704251033858	38.0	35.0	39.0	33.0	39.0
11	37.05191934119105	39.0	37.0	39.0	34.0	39.0
12	37.03653958454993	39.0	37.0	39.0	33.0	39.0
13	36.99489923607718	39.0	37.0	39.0	33.0	39.0
14	38.199043010880835	40.0	37.0	41.0	33.0	41.0
15	38.25340547497885	40.0	37.0	41.0	34.0	41.0
16	38.174689246683904	40.0	37.0	41.0	33.0	41.0
17	38.02085592725452	40.0	37.0	41.0	33.0	41.0
18	37.924328737084224	40.0	36.0	41.0	33.0	41.0
19	37.82596622531552	40.0	36.0	41.0	33.0	41.0
20	37.913966320656904	40.0	36.0	41.0	33.0	41.0
21	37.84015421468496	40.0	36.0	41.0	33.0	41.0
22	37.85393104434566	40.0	36.0	41.0	33.0	41.0
23	37.79176846345446	40.0	36.0	41.0	33.0	41.0
24	37.74402030771431	40.0	36.0	41.0	33.0	41.0
25	37.65919031331561	40.0	35.0	41.0	33.0	41.0
26	37.524508693942245	40.0	35.0	41.0	33.0	41.0
27	37.41475288705622	39.0	35.0	41.0	33.0	41.0
28	37.35646354979799	39.0	35.0	41.0	33.0	41.0
29	37.35107676172997	39.0	35.0	41.0	33.0	41.0
30	37.201760240260285	39.0	35.0	41.0	32.0	41.0
31	37.09014527643042	39.0	35.0	41.0	32.0	41.0
32	36.93043058551526	39.0	35.0	41.0	31.0	41.0
33	36.791172579818614	39.0	35.0	41.0	31.0	41.0
34	36.60144323016601	39.0	35.0	41.0	30.0	41.0
35	36.51335375227926	39.0	35.0	41.0	30.0	41.0
36	36.35748251081529	39.0	35.0	41.0	30.0	41.0
37	36.335965152724974	39.0	35.0	41.0	30.0	41.0
38	36.24645747178491	39.0	35.0	41.0	30.0	41.0
39	36.20249913596873	39.0	35.0	40.0	30.0	41.0
40	36.02381746892467	38.0	35.0	40.0	29.0	41.0
41	35.92103945941437	38.0	35.0	40.0	29.0	41.0
42	35.867004731316065	38.0	35.0	40.0	29.0	41.0
43	35.83691260770597	38.0	35.0	40.0	29.0	41.0
44	35.66881383403449	38.0	35.0	40.0	28.0	41.0
45	35.59258244050102	38.0	35.0	40.0	28.0	41.0
46	35.434869918602296	38.0	34.0	40.0	27.0	41.0
47	35.28508264906029	38.0	34.0	40.0	27.0	41.0
48	35.23392008008676	38.0	34.0	40.0	27.0	41.0
49	35.08325090276371	37.0	34.0	40.0	26.0	41.0
50	34.891704108021784	37.0	34.0	40.0	26.0	41.0
51	32.85311468376455	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	8.0
9	10.0
10	17.0
11	15.0
12	6.0
13	4.0
14	5.0
15	12.0
16	20.0
17	32.0
18	76.0
19	106.0
20	238.0
21	331.0
22	448.0
23	731.0
24	993.0
25	1303.0
26	1667.0
27	2018.0
28	2262.0
29	2786.0
30	3301.0
31	4338.0
32	5516.0
33	7878.0
34	12476.0
35	15429.0
36	15880.0
37	24562.0
38	31518.0
39	33825.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.63525962650014	19.589078644722257	23.010642481736166	12.765019247041437
2	30.620076511458844	26.652683264012207	24.41811962959873	18.309120594930224
3	32.99705633483893	24.886484167371794	25.765412530241093	16.35104696754818
4	29.00404009105102	25.496669010475635	26.98399456554124	18.515296332932106
5	25.785672573859774	30.507454504284404	23.967631600900976	19.739241320954847
6	26.203982886221976	35.63443730708267	24.356147731470998	13.80543207522435
7	60.5322432635355	28.120940542730814	7.107104124706527	4.239712069027161
8	81.3250068526618	6.626226030580748	8.090908007484298	3.9578591092731408
9	75.13913882896948	8.337603832723547	9.752827467852079	6.7704298704548975
10	43.69376348186726	27.655555423136967	15.812964044381413	12.837717050614355
11	34.890774529549866	24.974079061840804	22.392711151366363	17.742435257242967
12	33.586385250688245	21.950565493570416	25.70642005029258	18.75662920544876
13	26.6580462167348	23.906255586409088	26.805229474788163	22.630468722067953
14	20.859502556340797	26.957775685564123	28.05360569188049	24.12911606621459
15	21.980359675362596	26.025813679104743	32.21227758643292	19.78154905909974
16	25.892931628311622	24.969311992754058	29.39493975616442	19.742816622769904
17	24.33648357148816	25.62359222491032	28.012489721007285	22.027434482594238
18	25.886972791953188	27.301600543445876	25.991252428225813	20.820174236375124
19	26.059183162711985	29.14466862911011	23.966439833629288	20.82970837454862
20	29.707778664982303	25.817254406559485	26.009128937301124	18.465837991157088
21	26.450082827825383	29.28648893444088	25.425162974174402	18.838265263559332
22	25.445423017793082	24.729170887509085	27.234861576231395	22.590544518466434
23	26.9136802965117	26.022238377289682	27.53757046323994	19.52651086295868
24	26.69677865306463	23.33301552872755	28.04585920461452	21.924346613593297
25	23.77218176834428	26.010916588208655	25.956691177346887	24.26021046610018
26	22.34742399504225	27.11151366361177	27.114493081790986	23.426569259554995
27	22.682310598386344	28.585133895052973	25.58247625403711	23.150079252523568
28	20.683716883766937	30.108808351905044	27.75625975759454	21.451215006733484
29	24.521207498599672	26.311241940673824	26.574026624080847	22.593523936645653
30	22.97310181267802	27.017959932784326	27.764006244860507	22.24493200967715
31	25.64921522125159	27.772944499398157	24.725595585694027	21.85224469365622
32	26.721209882134218	27.51015981599113	24.982421432742612	20.786208869132036
33	25.246397883421324	27.737191481247542	24.765519789295546	22.25089084603559
34	22.610208678449272	27.942175451977736	28.499326651491497	20.94828921808149
35	23.262105376062163	27.765198012132192	26.520397096854925	22.45229951495072
36	22.047694526212922	32.67229975330417	24.67494547664732	20.605060243835585
37	24.061781215364263	31.294616787233785	24.78101276382748	19.862589233574468
38	22.831281507347246	30.098678330095694	24.34423005875413	22.725810103802928
39	24.743472094769334	27.122239569056955	25.533613795897935	22.600674540275776
40	24.83523817468925	25.677817635772087	26.13783980264334	23.349104386895327
41	20.783825334588663	28.36763636797006	27.46963972875377	23.378898568687507
42	22.57147624211944	26.2671465516214	26.616930245861585	24.544446960397572
43	23.350892037802858	26.503712355051306	25.792227293854058	24.353168313291782
44	22.0542492462072	27.10793836179671	25.652194639430814	25.185617752565282
45	23.135778045263322	26.770072340273394	25.48415545412292	24.609994160340367
46	22.566113289396846	29.22809233812821	23.95630981181995	24.249484560654995
47	21.152677305175846	27.031069372772887	28.79011786578317	23.026135456268097
48	22.451107747679032	25.694502377575706	28.641146956822272	23.213242917922987
49	22.404628824083233	25.748727788437474	27.70560964854783	24.141033738931462
50	19.921581713522983	25.896506930126684	28.880096294795553	25.301815061554777
51	19.823260913608788	25.185617752565282	27.198512674444935	27.792608659380996
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	100.0
1	230.0
2	360.0
3	783.5
4	1207.0
5	859.5
6	512.0
7	446.0
8	380.0
9	386.5
10	393.0
11	362.0
12	331.0
13	353.5
14	376.0
15	377.0
16	378.0
17	411.5
18	445.0
19	443.0
20	441.0
21	533.5
22	626.0
23	674.0
24	722.0
25	859.5
26	1236.0
27	1475.0
28	1829.0
29	2183.0
30	2576.0
31	2969.0
32	3505.5
33	4042.0
34	4613.0
35	5184.0
36	5152.0
37	5120.0
38	5662.5
39	6205.0
40	6745.5
41	7286.0
42	8184.0
43	9082.0
44	11335.5
45	13589.0
46	12745.0
47	11901.0
48	11800.5
49	11700.0
50	10902.5
51	10105.0
52	9895.5
53	9686.0
54	9465.5
55	9245.0
56	9571.5
57	9898.0
58	9801.0
59	9704.0
60	9671.0
61	9638.0
62	8473.0
63	7308.0
64	6148.5
65	4989.0
66	4118.0
67	3247.0
68	2678.5
69	2110.0
70	1806.0
71	1502.0
72	1231.5
73	961.0
74	765.5
75	460.0
76	350.0
77	278.0
78	206.0
79	172.5
80	139.0
81	108.5
82	78.0
83	58.0
84	38.0
85	29.0
86	20.0
87	16.5
88	13.0
89	7.0
90	1.0
91	1.5
92	2.0
93	2.5
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	167818.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.64360199740195
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.95808862786973	29.892502592093816
2	10.931660437800321	9.760573955118044
3	6.0744794447410575	8.135599280172567
4	4.251201281366791	7.591557520647369
5	3.000533902829685	6.69773206688198
6	2.08756006406834	5.591772038756272
7	1.6791243993593168	5.2473512972386755
8	1.2506673785371063	4.466743734283569
9	0.9917245061398825	3.984673872886103
>10	2.7335824879871864	15.834415855271782
>50	0.022690870261612386	0.7055262248388134
>100	0.01601708489054992	1.4569354896375837
>500	0.0026695141484249867	0.634616072173426
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCG	541	0.3223730469913835	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTC	524	0.31224302518204244	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	500	0.29794181792179625	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGC	477	0.2842364942973936	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	227	0.1352655853364955	No Hit
GCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC	193	0.11500554171781335	Illumina Single End Adapter 1 (95% over 21bp)
GAGGAGCTGAGAACCCCAGTCTAATAATGTCCATCGACACCTCCTTATCCC	180	0.10725905445184664	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06375954903526439	0.0
2	0.0	0.0	0.0	0.3170100942687912	0.0
3	0.0	0.0	0.0	0.4081802905528608	0.0
4	0.0	0.0	0.0	0.5690688722306309	0.0
5	0.0	0.0	0.0	1.2549309370866057	0.0
6	0.0	0.0	0.0	1.5189073877653172	0.0
7	0.0	0.0	0.0	1.833533947490734	0.0
8	0.0	0.0	0.0	2.3722127542933418	0.0
9	0.0	0.0	0.0	2.5944773504630017	0.0
10	0.0	0.0	0.0	3.4352691606383106	0.0
11	0.0	0.0	0.0	4.014468054678282	0.0
12	0.0	0.0	0.0	4.854068097581904	0.0
13	0.0	0.0	0.0	5.057860301040413	0.0
14	0.0	0.0	0.0	5.1508181482320134	0.0
15	0.0	0.0	0.0	5.34269267897365	0.0
16	0.0	0.0	0.0	5.662682191421659	0.0
17	0.0	0.0	0.0	6.030342394737156	0.0
18	0.0	0.0	0.0	6.439714452561704	0.0
19	0.0	0.0	0.0	6.7126291577780695	0.0
20	0.0	0.0	0.0	7.001632721162212	0.0
21	0.0	0.0	0.0	7.336519324506311	0.0
22	0.0	0.0	0.0	7.675577113301315	0.0
23	0.0	0.0	0.0	7.99497074211348	0.0
24	0.0	0.0	0.0	8.273248400052438	0.0
25	0.0	0.0	0.0	8.533649548916088	0.0
26	0.0	0.0	0.0	8.778557723247804	0.0
27	0.0	0.0	0.0	9.015719410313555	0.0
28	0.0	0.0	0.0	9.276716442813047	0.0
29	0.0	0.0	0.0	9.515665780786328	0.0
30	5.958836358435925E-4	0.0	0.0	9.830292340511745	0.0
31	5.958836358435925E-4	0.0	0.0	10.12644650752601	0.0
32	5.958836358435925E-4	0.0	0.0	10.426175976355337	0.0
33	5.958836358435925E-4	0.0	0.0	10.715775423375323	0.0
34	5.958836358435925E-4	0.0	0.0	10.963663015886258	0.0
35	5.958836358435925E-4	0.0	0.0	11.238961255645997	0.0
36	5.958836358435925E-4	0.0	0.0	11.532136004481044	0.0
37	5.958836358435925E-4	0.0	0.0	11.860467887830865	0.0
38	5.958836358435925E-4	0.0	0.0	12.212635116614427	0.0
39	5.958836358435925E-4	0.0	0.0	12.535604047241655	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTCGG	20	7.016057E-4	45.000004	24
CACGCCG	30	2.1543947E-6	45.000004	26
CTACATT	20	7.016057E-4	45.000004	22
CGTAACC	30	2.1543947E-6	45.000004	35
CGGTCTA	30	2.1543947E-6	45.000004	31
TAGGAAC	20	7.016057E-4	45.000004	6
GCGAGAC	55	1.8189894E-12	45.0	21
ACCGGAG	25	3.8765313E-5	45.0	32
CGACACC	55	1.8189894E-12	45.0	35
ACCTCCT	50	2.1827873E-11	45.0	39
CCATCGA	55	1.8189894E-12	45.0	31
TCGTCCC	25	3.8765313E-5	45.0	38
CGAATAT	35	1.2036435E-7	45.0	14
AACGACG	25	3.8765313E-5	45.0	13
CACCGGA	25	3.8765313E-5	45.0	31
GCTCGTA	25	3.8765313E-5	45.0	32
TCGACAC	55	1.8189894E-12	45.0	34
TAGCTCG	25	3.8765313E-5	45.0	30
ACACGAC	60	3.6379788E-12	41.250004	26
GTAGGGT	60	3.6379788E-12	41.250004	4
>>END_MODULE