FastQCFastQC Report
Sat 18 Jun 2016
SRR3551167_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551167_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143322
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTC6680.46608336473116474No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCG6510.4542219617365094No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGC4820.33630566137787643No Hit
GCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC3610.25188038123944684No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3530.2462985445360796No Hit
CTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGCT1950.13605726964457654Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC1830.12768451458952568No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCG1730.12070721871031663No Hit
GAATGACTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCT1690.117916300358633No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT1630.11372992283110758No Hit
GAATCTATCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTC1570.10954354530358214No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTTG207.0126134E-445.01
CACGGGC207.0126134E-445.04
GCGCGGG207.0126134E-445.03
AGCTTAG207.0126134E-445.02
CGTTGAT207.0126134E-445.025
GCGTTGG351.2020064E-745.02
ACGGGCG253.873679E-545.05
ACGGGAC253.873679E-545.05
TGATTCA207.0126134E-445.044
TACGAAT253.873679E-545.012
CGGGATT253.873679E-545.06
CGAATAT253.873679E-545.014
TAGGATG253.873679E-545.06
GGCGATA351.2020064E-745.08
AGCTACG253.873679E-545.09
TAGCTAG253.873679E-545.01
AAGCTAC253.873679E-545.08
ACGGGCA302.1521773E-644.9999965
CGTTGGG451.9081199E-840.03
ACGGGAG451.9081199E-840.05