##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551167_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	143322
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.878769484098743	31.0	31.0	34.0	30.0	34.0
2	31.981852053418176	33.0	31.0	34.0	30.0	34.0
3	31.8893889284269	33.0	31.0	34.0	30.0	34.0
4	35.659410278952286	37.0	35.0	37.0	33.0	37.0
5	35.74697534223636	37.0	35.0	37.0	35.0	37.0
6	35.71789397301182	37.0	35.0	37.0	35.0	37.0
7	26.867033672429912	35.0	11.0	37.0	0.0	37.0
8	31.128138038821675	35.0	19.0	37.0	17.0	37.0
9	35.837184800658655	39.0	32.0	39.0	32.0	39.0
10	36.559753561909545	37.0	35.0	39.0	32.0	39.0
11	36.92387770195783	39.0	37.0	39.0	33.0	39.0
12	37.02999539498472	39.0	37.0	39.0	33.0	39.0
13	36.982933534279454	39.0	37.0	39.0	33.0	39.0
14	38.17443239698023	40.0	37.0	41.0	33.0	41.0
15	38.213672709004896	40.0	37.0	41.0	33.0	41.0
16	38.170371610778524	40.0	37.0	41.0	33.0	41.0
17	38.05630677774522	40.0	37.0	41.0	33.0	41.0
18	37.967416028244095	40.0	37.0	41.0	33.0	41.0
19	37.86715926375574	40.0	37.0	41.0	33.0	41.0
20	37.939604526869566	40.0	37.0	41.0	33.0	41.0
21	37.89907341510724	40.0	37.0	41.0	33.0	41.0
22	37.897803547257226	40.0	36.0	41.0	33.0	41.0
23	37.88101617337185	40.0	36.0	41.0	33.0	41.0
24	37.806331198280795	40.0	36.0	41.0	33.0	41.0
25	37.721724508449505	39.0	36.0	41.0	33.0	41.0
26	37.56479116953434	39.0	36.0	41.0	33.0	41.0
27	37.47028369685045	39.0	36.0	41.0	33.0	41.0
28	37.46710204992953	39.0	36.0	41.0	33.0	41.0
29	37.47478405269254	39.0	36.0	41.0	33.0	41.0
30	37.3987175730174	39.0	36.0	41.0	32.0	41.0
31	37.28158272979724	39.0	36.0	41.0	32.0	41.0
32	37.19186168208649	39.0	36.0	41.0	32.0	41.0
33	37.135478154086606	39.0	35.0	41.0	32.0	41.0
34	37.00830298209626	39.0	35.0	41.0	31.0	41.0
35	36.943574608224836	39.0	35.0	41.0	31.0	41.0
36	36.74235637236433	39.0	35.0	41.0	30.0	41.0
37	36.712458659521914	39.0	35.0	41.0	30.0	41.0
38	36.62780312861947	39.0	35.0	41.0	30.0	41.0
39	36.5997055581139	39.0	35.0	41.0	30.0	41.0
40	36.42971072131285	39.0	35.0	40.0	30.0	41.0
41	36.302340185037885	39.0	35.0	40.0	30.0	41.0
42	36.2547829363252	39.0	35.0	40.0	30.0	41.0
43	36.24489610806436	39.0	35.0	40.0	30.0	41.0
44	36.08220650004884	39.0	35.0	40.0	29.0	41.0
45	36.00785643515999	38.0	35.0	40.0	29.0	41.0
46	35.861989087509244	38.0	35.0	40.0	29.0	41.0
47	35.71366573170902	38.0	35.0	40.0	28.0	41.0
48	35.59173748621984	38.0	35.0	40.0	28.0	41.0
49	35.418937776475346	38.0	34.0	40.0	27.0	41.0
50	35.18244930994544	38.0	34.0	40.0	26.0	41.0
51	32.86979668159808	36.0	31.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	9.0
9	8.0
10	10.0
11	6.0
12	5.0
13	5.0
14	4.0
15	5.0
16	13.0
17	29.0
18	41.0
19	78.0
20	134.0
21	203.0
22	355.0
23	461.0
24	740.0
25	1003.0
26	1252.0
27	1583.0
28	1886.0
29	2160.0
30	2800.0
31	3688.0
32	4795.0
33	6597.0
34	9877.0
35	12376.0
36	14134.0
37	21799.0
38	27800.0
39	29462.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.61647200011164	20.960494550731916	23.55255996985808	11.870473479298363
2	30.591953782392096	26.884218752180406	24.89289850825414	17.63092895717336
3	32.15486805933492	25.777619625737845	26.081829726071366	15.985682588855862
4	29.204867361605334	25.038723992129608	27.15703102105748	18.599377625207573
5	25.82018113060102	29.257197080699406	24.80010047306066	20.12252131563891
6	25.990427150053723	35.10277556830075	24.431699250638424	14.475098031007102
7	59.214216938083474	28.041054408953265	7.99109697045813	4.753631682505128
8	78.44155119241987	7.012182358605099	9.428419921575195	5.11784652739984
9	71.66520143453204	9.043273189042853	11.04505937678793	8.24646599963718
10	38.81469697603996	28.02640208760693	18.825442011693948	14.333458924659158
11	29.105789760120565	26.915616583636847	25.021280752431586	18.957312903811
12	29.097417005065516	22.808082499546476	29.09253289795007	19.00196759743794
13	24.27052371582869	25.57178939730118	29.913760622932976	20.243926263937148
14	19.069647367466263	29.458840931608542	28.210602698818043	23.260909002107145
15	18.190508086685924	27.61125298279399	34.857174753352595	19.341064177167496
16	20.680704985975634	26.582799570198574	32.13602936046106	20.600466083364733
17	19.81482256736579	26.51930617769777	29.35557695259625	24.310294302340186
18	21.326802584390396	27.83592191010452	29.604666415484015	21.23260909002107
19	23.207183823837234	29.08067149495541	26.208816511072968	21.503328170134385
20	25.47131633664057	27.234479005316697	28.209904969230127	19.084299688812603
21	23.987245503132808	28.238511882334883	27.52194359554011	20.252299018992197
22	21.661712786592428	26.808166227097026	27.518454947600507	24.01166603871004
23	21.467046231562495	28.732504430582885	27.847085583511255	21.953363754343368
24	22.236641967039255	25.257811082736776	29.27952442751287	23.226022522711098
25	21.089574524497287	28.856002567644882	26.45441732602113	23.600005581836704
26	20.294162794267454	30.344957508268095	27.02655558811627	22.33432410934818
27	21.873124851732463	29.189517310671075	27.849178772275017	21.088179065321444
28	19.895061469976696	29.03252815338887	29.039505449268084	22.03290492736635
29	21.143299702767195	27.620323467436958	28.62365861486722	22.612718214928623
30	21.240981845076124	27.084467143913706	28.2859574943135	23.38859351669667
31	23.060660610373844	28.10664099021783	26.241609801705252	22.591088597703074
32	23.389988975872512	29.035319071740556	25.497132331393647	22.077559620993288
33	22.004995743849516	27.268667755124827	26.793513905750686	23.932822595274974
34	21.233306819608995	27.298670127405423	28.890191317453006	22.577831735532577
35	21.7775358981873	25.813901564309738	27.990120149035043	24.418442388467927
36	22.347580971518678	27.939883618704737	28.02012252131564	21.69241288846095
37	21.665899164119953	27.872203848676406	30.211691156975203	20.25020583022844
38	21.994529800030698	26.614197401655016	26.605126917012043	24.786145881302243
39	21.97010926445347	25.143383430317744	28.9599642762451	23.926543028983687
40	22.620393240395753	24.494494913551303	30.22564574873362	22.65946609731932
41	19.517589762911484	26.698622681793445	30.53892633371011	23.244861221584962
42	21.230515901257306	26.305800923793974	29.864919551778513	22.598763623170203
43	22.85692357070094	26.653270258578587	27.824060507109866	22.66574566361061
44	21.100738197904022	27.034928343171323	26.985389542428933	24.87894391649572
45	20.458826977016788	25.86971993134341	26.37417842341022	27.297274668229583
46	22.007786662201198	27.487057116144065	26.871659619597825	23.63349660205691
47	20.479758864654414	26.284171306568428	31.324569849709043	21.911499979068115
48	21.64147862854272	24.477051673853282	29.29836312638674	24.583106571217257
49	19.846918128410156	24.78335496295056	31.323174390533204	24.046552518106083
50	19.185470479061134	24.082834456677972	30.513808068544957	26.21788699571594
51	19.25873208579283	24.440769735281396	28.01244749584851	28.288050683077266
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	56.0
1	203.0
2	350.0
3	564.0
4	778.0
5	568.5
6	359.0
7	347.0
8	335.0
9	312.0
10	289.0
11	300.0
12	311.0
13	316.5
14	322.0
15	327.5
16	333.0
17	355.5
18	378.0
19	410.5
20	443.0
21	449.5
22	456.0
23	586.0
24	716.0
25	846.0
26	1136.5
27	1297.0
28	1716.5
29	2136.0
30	2634.5
31	3133.0
32	3379.0
33	3625.0
34	4086.0
35	4547.0
36	4776.0
37	5005.0
38	5602.0
39	6199.0
40	6803.0
41	7407.0
42	8074.0
43	8741.0
44	9751.5
45	10762.0
46	13148.5
47	15535.0
48	14592.0
49	13649.0
50	12779.0
51	11909.0
52	10306.5
53	8704.0
54	7622.5
55	6541.0
56	6193.0
57	5845.0
58	5784.5
59	5724.0
60	5433.5
61	5143.0
62	4701.5
63	4260.0
64	3488.5
65	2717.0
66	2170.5
67	1624.0
68	1328.0
69	1032.0
70	827.0
71	622.0
72	538.5
73	455.0
74	366.0
75	205.0
76	133.0
77	106.0
78	79.0
79	66.0
80	53.0
81	39.0
82	25.0
83	22.0
84	19.0
85	15.5
86	12.0
87	7.5
88	3.0
89	3.5
90	4.0
91	2.5
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	143322.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.514226706297706
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.6677808779248	37.21270984217357
2	9.75026934445703	9.850546322267343
3	4.634105914528026	7.022648302423913
4	3.1395894914224147	6.3437574133768715
5	2.265255946296859	5.721382620951424
6	1.5483853145114506	4.692929208356009
7	1.2251719660764109	4.332203011400901
8	0.8991961103897896	3.6337756938920753
9	0.7679770159396668	3.4914388579562106
>10	2.049780380673499	13.788532116492933
>50	0.030387579767396887	1.0375238972383862
>100	0.019337550761070747	1.952247387002693
>500	0.0027625072515815355	0.9203053264676742
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTC	668	0.46608336473116474	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCG	651	0.4542219617365094	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGC	482	0.33630566137787643	No Hit
GCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC	361	0.25188038123944684	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	353	0.2462985445360796	No Hit
CTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGCT	195	0.13605726964457654	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC	183	0.12768451458952568	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCG	173	0.12070721871031663	No Hit
GAATGACTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCT	169	0.117916300358633	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	163	0.11372992283110758	No Hit
GAATCTATCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTC	157	0.10954354530358214	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.18978244791448626	0.0
2	0.0	0.0	0.0	0.9133280305884651	0.0
3	0.0	0.0	0.0	1.1840471107017765	0.0
4	0.0	0.0	0.0	1.5810552462287715	0.0
5	0.0	0.0	0.0	3.0867556969620855	0.0
6	0.0	0.0	0.0	3.6456370968867304	0.0
7	0.0	0.0	0.0	4.337784848104269	0.0
8	0.0	0.0	0.0	5.466711321360294	0.0
9	0.0	0.0	0.0	5.863719456887289	0.0
10	0.0	0.0	0.0	7.326858402757427	0.0
11	0.0	0.0	0.0	8.596726252773475	0.0
12	0.0	0.0	0.0	9.967764893038055	0.0
13	0.0	0.0	0.0	10.412916370131592	0.0
14	0.0	0.0	0.0	10.595023792578948	0.0
15	0.0	0.0	0.0	10.970402310880395	0.0
16	0.0	0.0	0.0	11.713484322016159	0.0
17	0.0	0.0	0.0	12.568203067219269	0.0
18	0.0	0.0	0.0	13.450133266351292	0.0
19	0.0	0.0	0.0	13.993664615341679	0.0
20	0.0	0.0	0.0	14.476493490182945	0.0
21	0.0	0.0	0.0	15.141429787471568	0.0
22	0.0	0.0	0.0	15.869859477260993	0.0
23	0.0	0.0	0.0	16.62410516180349	0.0
24	0.0	0.0	0.0	17.212291204420815	0.0
25	0.0	0.0	0.0	17.7090746710205	0.0
26	0.0	0.0	0.0	18.154923877701957	0.0
27	0.0	0.0	0.0	18.630077727076095	0.0
28	0.0	0.0	0.0	19.132443030379147	0.0
29	0.0	0.0	0.0	19.623644660275463	0.0
30	0.0	0.0	0.0	20.16647827967793	0.0
31	0.0	0.0	0.0	20.72326649083881	0.0
32	0.0	0.0	0.0	21.310754803868214	0.0
33	0.0	0.0	0.0	21.78660638283027	0.0
34	0.0	0.0	0.0	22.259667043440643	0.0
35	0.0	0.0	0.0	22.743891377457754	0.0
36	0.0	0.0	0.0	23.278352241805166	0.0
37	0.0	0.0	0.0	23.75978565747059	0.0
38	0.0	0.0	0.0	24.247498639427302	0.0
39	0.0	0.0	0.0	24.739397998911542	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTTG	20	7.0126134E-4	45.0	1
CACGGGC	20	7.0126134E-4	45.0	4
GCGCGGG	20	7.0126134E-4	45.0	3
AGCTTAG	20	7.0126134E-4	45.0	2
CGTTGAT	20	7.0126134E-4	45.0	25
GCGTTGG	35	1.2020064E-7	45.0	2
ACGGGCG	25	3.873679E-5	45.0	5
ACGGGAC	25	3.873679E-5	45.0	5
TGATTCA	20	7.0126134E-4	45.0	44
TACGAAT	25	3.873679E-5	45.0	12
CGGGATT	25	3.873679E-5	45.0	6
CGAATAT	25	3.873679E-5	45.0	14
TAGGATG	25	3.873679E-5	45.0	6
GGCGATA	35	1.2020064E-7	45.0	8
AGCTACG	25	3.873679E-5	45.0	9
TAGCTAG	25	3.873679E-5	45.0	1
AAGCTAC	25	3.873679E-5	45.0	8
ACGGGCA	30	2.1521773E-6	44.999996	5
CGTTGGG	45	1.9081199E-8	40.0	3
ACGGGAG	45	1.9081199E-8	40.0	5
>>END_MODULE