FastQCFastQC Report
Sat 18 Jun 2016
SRR3551166_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551166_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96161
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2330.24230197273322868No Hit
GAGGAGCTGAGAACCCCAGTCTAATAATGTCCATCGACACCTCCTTATCCC1360.14142947764686306No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCG1010.10503218560539095No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC207.001047E-445.025
AAGTACG253.8641076E-545.01
TTGGCGG207.001047E-445.02
AGTCCGT207.001047E-445.020
ACGCCGG207.001047E-445.027
TGCCAAT253.8641076E-545.014
GACAATA207.001047E-445.021
GACCGAT207.001047E-445.09
GTCTCAC207.001047E-445.022
CACGCCG207.001047E-445.026
GTAGGGT351.1964585E-745.04
CACGACG207.001047E-445.026
CGACAAT207.001047E-445.020
GTCAGAC207.001047E-445.033
GTCCGTT207.001047E-445.021
GATGCGG253.8641076E-545.02
GCAGGGT253.8641076E-545.04
ACCACCT207.001047E-445.040
CGGTCTA207.001047E-445.031
TGGGCTG207.001047E-445.06