##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551166_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	96161
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.126319401836504	33.0	31.0	34.0	30.0	34.0
2	32.25679849419203	33.0	31.0	34.0	30.0	34.0
3	32.1378729422531	33.0	31.0	34.0	30.0	34.0
4	35.88847869718493	37.0	35.0	37.0	35.0	37.0
5	35.9641746654049	37.0	35.0	37.0	35.0	37.0
6	35.92821414086792	37.0	35.0	37.0	35.0	37.0
7	26.723027006790694	36.0	10.0	37.0	0.0	37.0
8	31.137352980938218	37.0	19.0	37.0	17.0	37.0
9	35.994696394588246	39.0	32.0	39.0	32.0	39.0
10	36.80100040556982	38.0	35.0	39.0	34.0	39.0
11	37.15541643701709	39.0	37.0	39.0	34.0	39.0
12	37.13931843470846	39.0	37.0	39.0	34.0	39.0
13	36.99590270483876	39.0	37.0	39.0	33.0	39.0
14	38.18539740643296	40.0	37.0	41.0	33.0	41.0
15	38.302835869011346	40.0	37.0	41.0	34.0	41.0
16	38.26054221565916	40.0	37.0	41.0	34.0	41.0
17	38.13911045018251	40.0	37.0	41.0	34.0	41.0
18	38.04296960306153	40.0	37.0	41.0	33.0	41.0
19	37.96349871569555	40.0	36.0	41.0	34.0	41.0
20	37.99138944062562	40.0	36.0	41.0	34.0	41.0
21	37.914622352097005	40.0	36.0	41.0	33.0	41.0
22	37.891598465074196	40.0	36.0	41.0	33.0	41.0
23	37.85451482409709	40.0	36.0	41.0	33.0	41.0
24	37.77477355684737	40.0	36.0	41.0	33.0	41.0
25	37.70575389191044	40.0	35.0	41.0	33.0	41.0
26	37.52781273073283	39.0	35.0	41.0	33.0	41.0
27	37.397489626771765	39.0	35.0	41.0	33.0	41.0
28	37.39488982019738	39.0	35.0	41.0	33.0	41.0
29	37.39378750220983	39.0	35.0	41.0	33.0	41.0
30	37.29187508449371	39.0	35.0	41.0	33.0	41.0
31	37.129262382878714	39.0	35.0	41.0	32.0	41.0
32	36.98767691683739	39.0	35.0	41.0	32.0	41.0
33	36.87754911034619	39.0	35.0	41.0	31.0	41.0
34	36.72946412786889	39.0	35.0	41.0	31.0	41.0
35	36.61595657283098	39.0	35.0	41.0	31.0	41.0
36	36.456869208930854	39.0	35.0	41.0	30.0	41.0
37	36.34687659238153	39.0	35.0	41.0	30.0	41.0
38	36.21717744199832	39.0	35.0	41.0	30.0	41.0
39	36.124187560445506	39.0	35.0	40.0	30.0	41.0
40	35.87779869177733	38.0	35.0	40.0	29.0	41.0
41	35.73011927912563	38.0	35.0	40.0	28.0	41.0
42	35.67188361185928	38.0	35.0	40.0	28.0	41.0
43	35.61583178211541	38.0	35.0	40.0	27.0	41.0
44	35.40059899543474	38.0	34.0	40.0	27.0	41.0
45	35.26991191855326	38.0	34.0	40.0	26.0	41.0
46	35.06313370285251	38.0	34.0	40.0	25.0	41.0
47	34.893667911107414	38.0	34.0	40.0	24.0	41.0
48	34.79803662607502	38.0	34.0	40.0	24.0	41.0
49	34.62906999719221	37.0	34.0	40.0	24.0	41.0
50	34.42792816214474	37.0	34.0	40.0	23.0	41.0
51	32.238090286082716	35.0	30.0	39.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	8.0
9	10.0
10	6.0
11	8.0
12	3.0
13	5.0
14	5.0
15	6.0
16	17.0
17	24.0
18	46.0
19	73.0
20	124.0
21	180.0
22	275.0
23	431.0
24	558.0
25	843.0
26	990.0
27	1176.0
28	1391.0
29	1547.0
30	1896.0
31	2413.0
32	3041.0
33	4447.0
34	7218.0
35	9261.0
36	9110.0
37	14113.0
38	17661.0
39	19273.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.36003161364795	21.505599983361236	25.752644003286157	13.38172439970466
2	31.971381329229104	25.4562660538056	25.724566092282735	16.847786524682565
3	32.11385072950573	24.297792244257028	26.58250226183172	17.00585476440553
4	29.219746050893814	25.152608645916747	25.95958860660767	19.668056696581772
5	25.70792733020663	29.065837501689874	24.368506983080458	20.857728185023035
6	26.642817774357585	34.25817119206331	24.26555464273458	14.833456390844521
7	59.86730587244309	28.702904503904907	6.957082393069955	4.472707230582045
8	80.62520148500951	5.739332993625275	9.202275350713906	4.4331901706513035
9	74.77875646051933	8.332900032237601	10.865111635694303	6.023231871548757
10	44.52636723827747	23.488732438306588	18.20280571125508	13.782094612160856
11	34.55974875469265	24.998700096712806	23.161156809933342	17.280394338661203
12	31.655244849783177	23.279707989725566	27.230374060169922	17.834673100321336
13	25.871195183078378	25.85039673048325	28.269256767296515	20.009151319141854
14	20.284730816027288	28.83913436840299	29.878017075529577	20.99811774004014
15	19.804286561079856	25.7516040806564	35.02251432493422	19.42159503332952
16	23.286987448133857	23.617682844396377	33.384636183067975	19.710693524401783
17	23.208993250902132	24.076288724118925	28.613471157745863	24.10124686723308
18	24.11996547456869	26.039662649098904	29.154230925219167	20.686140951113238
19	25.95750876134816	26.22892856771456	27.143020559270393	20.670542111666894
20	27.522592319131455	26.003265357057437	27.394681835671424	19.07946048813968
21	25.750564158026645	25.65801104397833	28.13302690279843	20.458397895196597
22	23.466894063081707	24.500577157059514	28.850573517330307	23.18195526252847
23	24.27387402377263	25.124530734913325	28.78505839165566	21.816536849658384
24	24.401784507232662	24.290512785848733	29.55876082819438	21.748941878724224
25	23.462734372562682	25.550899013113426	27.358284543629953	23.62808207069394
26	21.566955418516862	28.73306226016784	27.640103576293924	22.05987874502137
27	22.05467913187259	28.026954794563284	29.08767587691476	20.830690196649368
28	20.210896309314588	27.538191158577803	30.9709757594035	21.27993677270411
29	22.920934682459627	27.458117116086562	28.892170422520564	20.728777778933246
30	22.44361019540146	26.420274331589727	29.887376379197388	21.24873909381142
31	24.297792244257028	26.269485550275057	27.638023731034412	21.794698474433503
32	26.565863499755622	26.401555724254116	26.83624338349227	20.196337392498
33	24.6867233077859	26.14053514418527	26.841442996641053	22.331298551387775
34	22.406172980730233	26.842482919270804	28.635309532970748	22.116034567028215
35	23.74143363733738	25.68504903235199	27.942721061553023	22.630796268757607
36	22.904295920383525	27.15133994030844	29.043999126464993	20.900365012843043
37	23.398259169517786	27.60682605214172	28.613471157745863	20.381443620594627
38	23.09564168425869	26.75616934100103	26.029263422801343	24.118925551938936
39	24.910306673183516	24.909266750553762	26.815444930897144	23.364981645365585
40	24.030532128409646	24.66072524204199	28.081030771310616	23.227711858237747
41	20.730857624192762	26.269485550275057	28.96496500660351	24.03469181892867
42	22.081717120246253	26.097898316365264	28.448123459614607	23.372261103773877
43	23.54488826031343	24.84895123802789	28.349330809787755	23.256829691870927
44	22.098355882322355	26.48578945726438	27.971838895186195	23.444015765227068
45	23.045725398030388	26.787367019893722	26.755129418371272	23.411778163704618
46	23.132038976300162	26.463951082039493	27.743055916639804	22.660954025020537
47	21.528478281215875	26.11037738792234	29.781304270962238	22.579840059899546
48	23.55944717713002	24.3341895362985	28.923368101413256	23.182995185158227
49	22.33857800979607	23.225632012978235	29.299820093385055	25.13596988384064
50	20.840049500317175	24.518255841765374	29.796903110408586	24.844791547508866
51	21.532637971734903	25.38139162446314	27.399881448820206	25.686088954981752
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	98.0
1	197.0
2	296.0
3	473.0
4	650.0
5	482.5
6	315.0
7	294.5
8	274.0
9	255.5
10	237.0
11	245.0
12	253.0
13	256.0
14	259.0
15	270.5
16	282.0
17	275.5
18	269.0
19	262.0
20	255.0
21	292.0
22	329.0
23	375.0
24	421.0
25	555.0
26	836.0
27	983.0
28	1267.0
29	1551.0
30	1748.0
31	1945.0
32	2057.5
33	2170.0
34	2355.5
35	2541.0
36	2735.5
37	2930.0
38	3193.0
39	3456.0
40	4077.0
41	4698.0
42	5193.0
43	5688.0
44	6270.0
45	6852.0
46	7367.0
47	7882.0
48	7788.5
49	7695.0
50	7434.0
51	7173.0
52	6607.0
53	6041.0
54	5524.0
55	5007.0
56	4891.5
57	4776.0
58	4699.0
59	4622.0
60	4503.5
61	4385.0
62	3967.0
63	3549.0
64	2959.0
65	2369.0
66	2025.0
67	1681.0
68	1475.0
69	1269.0
70	1036.0
71	803.0
72	700.5
73	598.0
74	494.0
75	308.5
76	227.0
77	169.5
78	112.0
79	81.5
80	51.0
81	40.0
82	29.0
83	27.5
84	26.0
85	20.0
86	14.0
87	10.5
88	7.0
89	7.0
90	7.0
91	4.5
92	2.0
93	2.0
94	2.0
95	2.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	96161.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.005137217790995
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.57887836524334	32.00985846653009
2	9.64533556364294	8.874699722340658
3	5.501932683831009	7.593515042480839
4	3.7433034200592235	6.888447499506037
5	2.660548384909243	6.119944676116098
6	1.9846741562874386	5.478312413556432
7	1.5416261669567575	4.964590634456796
8	1.1799543389317118	4.342716901862501
9	0.9945975270688758	4.11809361383513
>10	3.1533262505933677	18.850677509593286
>50	0.009041795700626144	0.27037988373665
>100	0.006781346775469608	0.4887636359854826
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	233	0.24230197273322868	No Hit
GAGGAGCTGAGAACCCCAGTCTAATAATGTCCATCGACACCTCCTTATCCC	136	0.14142947764686306	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCG	101	0.10503218560539095	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0655151256746498	0.0
2	0.0	0.0	0.0	0.3650128430444775	0.0
3	0.0	0.0	0.0	0.5324403864352493	0.0
4	0.0	0.0	0.0	0.7622632876114017	0.0
5	0.0	0.0	0.0	1.4798099021432805	0.0
6	0.0	0.0	0.0	1.8521022035960524	0.0
7	0.0	0.0	0.0	2.225434427678581	0.0
8	0.0	0.0	0.0	2.859787231829952	0.0
9	0.0	0.0	0.0	3.1228876571583073	0.0
10	0.0	0.0	0.0	3.962105219371679	0.0
11	0.0	0.0	0.0	4.941712336602157	0.0
12	0.0	0.0	0.0	5.760131446220401	0.0
13	0.0	0.0	0.0	6.077307848296087	0.0
14	0.0	0.0	0.0	6.220817171202462	0.0
15	0.0	0.0	0.0	6.441280768710808	0.0
16	0.0	0.0	0.0	6.979960690924595	0.0
17	0.0	0.0	0.0	7.548798369401316	0.0
18	0.0	0.0	0.0	8.19355039985025	0.0
19	0.0	0.0	0.0	8.558563242894728	0.0
20	0.0	0.0	0.0	8.884059026008465	0.0
21	0.0	0.0	0.0	9.33226567943345	0.0
22	0.0	0.0	0.0	9.751354499225258	0.0
23	0.0	0.0	0.0	10.159004170089746	0.0
24	0.0	0.0	0.0	10.481380185314213	0.0
25	0.0	0.0	0.0	10.775678289535259	0.0
26	0.0	0.0	0.0	11.075176006905087	0.0
27	0.0	0.0	0.0	11.36739426586662	0.0
28	0.0	0.0	0.0	11.683530745312549	0.0
29	0.0	0.0	0.0	11.997587379498965	0.0
30	0.0	0.0	0.0	12.328282775761483	0.0
31	0.0	0.0	0.0	12.634020028909848	0.0
32	0.0	0.0	0.0	12.965755347802123	0.0
33	0.0	0.0	0.0	13.262133297282682	0.0
34	0.0	0.0	0.0	13.568910473060804	0.0
35	0.0	0.0	0.0	13.891286488285271	0.0
36	0.0	0.0	0.0	14.205343122471689	0.0
37	0.0	0.0	0.0	14.55475712606982	0.0
38	0.0	0.0	0.0	14.89065213548112	0.0
39	0.0	0.0	0.0	15.239026216449496	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	20	7.001047E-4	45.0	25
AAGTACG	25	3.8641076E-5	45.0	1
TTGGCGG	20	7.001047E-4	45.0	2
AGTCCGT	20	7.001047E-4	45.0	20
ACGCCGG	20	7.001047E-4	45.0	27
TGCCAAT	25	3.8641076E-5	45.0	14
GACAATA	20	7.001047E-4	45.0	21
GACCGAT	20	7.001047E-4	45.0	9
GTCTCAC	20	7.001047E-4	45.0	22
CACGCCG	20	7.001047E-4	45.0	26
GTAGGGT	35	1.1964585E-7	45.0	4
CACGACG	20	7.001047E-4	45.0	26
CGACAAT	20	7.001047E-4	45.0	20
GTCAGAC	20	7.001047E-4	45.0	33
GTCCGTT	20	7.001047E-4	45.0	21
GATGCGG	25	3.8641076E-5	45.0	2
GCAGGGT	25	3.8641076E-5	45.0	4
ACCACCT	20	7.001047E-4	45.0	40
CGGTCTA	20	7.001047E-4	45.0	31
TGGGCTG	20	7.001047E-4	45.0	6
>>END_MODULE