##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551164_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	279742
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.86566193135103	31.0	31.0	34.0	30.0	34.0
2	31.967966912369256	33.0	31.0	34.0	30.0	34.0
3	31.333003267296295	31.0	31.0	34.0	28.0	34.0
4	35.36820713371607	37.0	35.0	37.0	33.0	37.0
5	35.651543207669924	37.0	35.0	37.0	33.0	37.0
6	35.69826125501355	37.0	35.0	37.0	35.0	37.0
7	27.027114269576966	35.0	18.0	37.0	0.0	37.0
8	31.26874048230155	35.0	25.0	37.0	17.0	37.0
9	35.97681792508812	39.0	32.0	39.0	32.0	39.0
10	36.75356578561675	38.0	35.0	39.0	33.0	39.0
11	37.12355670582179	39.0	37.0	39.0	34.0	39.0
12	37.24205517941532	39.0	37.0	39.0	34.0	39.0
13	37.28311444116364	39.0	37.0	39.0	34.0	39.0
14	38.56812348521137	40.0	38.0	41.0	35.0	41.0
15	38.59154506652558	40.0	38.0	41.0	34.0	41.0
16	38.50991985472328	40.0	38.0	41.0	34.0	41.0
17	38.362655589793455	40.0	38.0	41.0	34.0	41.0
18	38.140733247063366	40.0	37.0	41.0	33.0	41.0
19	37.94675450951234	40.0	37.0	41.0	34.0	41.0
20	37.888247027618306	40.0	36.0	41.0	34.0	41.0
21	37.84822443537259	40.0	36.0	41.0	33.0	41.0
22	37.83576652772912	39.0	36.0	41.0	33.0	41.0
23	37.794167482894956	39.0	36.0	41.0	33.0	41.0
24	37.73241772776344	39.0	35.0	41.0	33.0	41.0
25	37.621469067926874	39.0	35.0	41.0	33.0	41.0
26	37.496968635385464	39.0	35.0	41.0	33.0	41.0
27	37.38550521552001	39.0	35.0	41.0	33.0	41.0
28	37.27641183662089	39.0	35.0	41.0	33.0	41.0
29	37.185556691522905	39.0	35.0	41.0	33.0	41.0
30	37.06052005061807	39.0	35.0	41.0	32.0	41.0
31	36.90694282588957	39.0	35.0	41.0	31.0	41.0
32	36.63761608911068	39.0	35.0	41.0	31.0	41.0
33	36.36169398946172	39.0	35.0	41.0	30.0	41.0
34	36.055247335044434	39.0	35.0	41.0	29.0	41.0
35	35.82924980875235	39.0	35.0	41.0	26.0	41.0
36	35.566389744836314	39.0	35.0	41.0	25.0	41.0
37	35.41401362684188	39.0	35.0	41.0	24.0	41.0
38	35.247774735291806	39.0	35.0	40.0	23.0	41.0
39	35.123395843312764	39.0	35.0	40.0	23.0	41.0
40	34.94522452831538	39.0	34.0	40.0	22.0	41.0
41	34.8215605808209	38.0	34.0	40.0	21.0	41.0
42	34.74707051497451	38.0	34.0	40.0	21.0	41.0
43	34.685159897333975	38.0	34.0	40.0	21.0	41.0
44	34.50659178814765	38.0	34.0	40.0	20.0	41.0
45	34.45100485447305	38.0	34.0	40.0	20.0	41.0
46	34.249215348428194	38.0	33.0	40.0	20.0	41.0
47	34.116378663196805	38.0	33.0	40.0	19.0	41.0
48	33.975227173609966	38.0	33.0	40.0	18.0	41.0
49	33.78919146928241	38.0	33.0	40.0	17.0	41.0
50	33.57253111795869	37.0	33.0	40.0	15.0	41.0
51	31.52056180337597	35.0	28.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	10.0
9	9.0
10	14.0
11	16.0
12	8.0
13	15.0
14	12.0
15	13.0
16	21.0
17	46.0
18	123.0
19	239.0
20	426.0
21	714.0
22	1158.0
23	1818.0
24	2722.0
25	4369.0
26	5352.0
27	5816.0
28	5720.0
29	5649.0
30	5817.0
31	7095.0
32	8900.0
33	11731.0
34	16929.0
35	22059.0
36	24956.0
37	39663.0
38	50931.0
39	57380.0
40	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.12977672283747	19.480807315311964	22.453546482115662	19.9358694797349
2	36.604800137269336	24.78712527972203	23.08448498974055	15.523589593268083
3	29.143282024150825	23.8180180308999	32.243996253690895	14.794703691258373
4	25.205367803190082	23.291818890263173	34.509297853021714	16.993515453525035
5	24.106498130420174	27.3995324263071	30.650027525362656	17.843941917910076
6	23.344724782120668	32.957868321524835	30.266102337153523	13.43130455920098
7	55.83215963280451	26.68852013641141	13.575723345082254	3.903596885701825
8	75.7733911961736	5.195144097060863	15.182561074132595	3.848903632632926
9	68.98141859284627	7.765369519056846	16.7972631925131	6.455948695583788
10	32.914971652451186	32.57251324434658	22.5239685138449	11.988546589357336
11	24.41964381465779	25.351573950282763	33.38004303965797	16.84873919540148
12	24.246269777151806	21.70106741211545	36.256264701045964	17.796398109686784
13	22.072838544087052	22.60725954629623	36.463241129326306	18.85666078029041
14	17.724903661230705	26.629179744192864	35.25248264472264	20.393433949853794
15	17.734912884014555	26.80326872618341	37.71224914385398	17.749569245948052
16	21.04474837528866	27.150016801195388	34.04029427114985	17.76494055236611
17	20.641519685996382	26.021476932316208	33.24849325449879	20.088510127188623
18	20.23042660737394	26.613093493290247	33.75431647732554	19.40216342201028
19	21.23885580284691	27.692659664977015	31.5204724353154	19.548012096860678
20	21.70070993987317	26.962701346240465	33.00541212974812	18.33117658413824
21	22.678396522510027	26.58020604700045	33.51159282481716	17.22980460567237
22	21.357536587284	24.123299325807352	33.5198146863896	20.999349400519048
23	20.738394663654365	25.3855338132994	34.15146813849905	19.72460338454719
24	21.05261276461883	24.64056166038707	33.91696634756311	20.38985922743099
25	20.330161362970163	28.047272129319158	31.393927261548143	20.228639246162537
26	19.377497837292935	27.82492439462076	31.889026317106477	20.908551450979832
27	20.14391832474208	27.58827777023114	31.7281638080803	20.53964009694647
28	19.57482251503171	27.489257959119474	32.9986201571448	19.93729936870402
29	20.2329289130699	26.90908050989841	32.66831580527772	20.189674771753975
30	20.220417384590085	27.739488528715743	32.48886473965297	19.5512293470412
31	21.826897641398148	27.585060520050618	31.07148729901123	19.516554539540003
32	21.161284326272064	27.83886581206969	32.11387635750084	18.885973504157402
33	20.626505851820607	26.409691787432703	31.635578497329682	21.328223863417005
34	19.98984778831924	26.387171036169043	33.675315111781565	19.947666063730153
35	19.123692545273858	26.658134995817573	33.43294893151547	20.7852235273931
36	20.170371270670834	27.423125594297602	31.62413938557671	20.782363749454856
37	19.793238055065025	27.54180637873469	32.87993937270771	19.785016193492574
38	19.910488950532994	26.507281709575253	31.368546732346232	22.213682607545522
39	21.244575358723395	25.21215977579341	31.87294006620386	21.670324799279335
40	21.08299790521266	25.750155500425393	32.75303672669817	20.413809867663776
41	18.826275639696576	27.370219702440107	31.723516668930657	22.079987988932658
42	19.968756926024696	26.797191698064644	31.751042031586245	21.483009344324415
43	21.04725068098462	26.843305617318812	30.282188588056137	21.827255113640426
44	20.577174682385913	26.638116550249872	30.034102851913545	22.750605915450663
45	20.329088946243324	25.790192391560794	29.69736399968542	24.183354662510457
46	21.159854437302943	27.553602962729943	28.990641376697102	22.29590122327001
47	20.04025137448077	25.929964038292425	32.371613844185	21.6581707430418
48	20.128547018324028	25.457028261755475	31.41037098469304	23.00405373522746
49	19.517626956266845	25.72870716588857	32.193592667529366	22.560073210315217
50	19.205911160998347	25.06452373973161	32.80272536837515	22.926839730894898
51	19.038614151611128	24.698829635878774	31.83647789749126	24.42607831501884
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	104.0
1	257.0
2	410.0
3	4798.5
4	9187.0
5	6220.5
6	3254.0
7	2859.5
8	2465.0
9	2370.0
10	2275.0
11	2198.5
12	2122.0
13	2060.0
14	1998.0
15	1919.0
16	1840.0
17	1800.5
18	1761.0
19	1707.5
20	1654.0
21	1664.0
22	1674.0
23	1750.5
24	1827.0
25	2044.0
26	2674.5
27	3088.0
28	3641.5
29	4195.0
30	4963.5
31	5732.0
32	6288.0
33	6844.0
34	7764.0
35	8684.0
36	9345.5
37	10007.0
38	11079.0
39	12151.0
40	13460.0
41	14769.0
42	16648.0
43	18527.0
44	23379.5
45	28232.0
46	25929.5
47	23627.0
48	22355.0
49	21083.0
50	18636.5
51	16190.0
52	14766.5
53	13343.0
54	12426.5
55	11510.0
56	10973.0
57	10436.0
58	10075.0
59	9714.0
60	9277.0
61	8840.0
62	7812.5
63	6785.0
64	5793.5
65	4802.0
66	3920.0
67	3038.0
68	2476.0
69	1914.0
70	1641.0
71	1368.0
72	1091.0
73	814.0
74	696.0
75	438.5
76	299.0
77	231.0
78	163.0
79	118.5
80	74.0
81	57.0
82	40.0
83	33.0
84	26.0
85	22.5
86	19.0
87	12.0
88	5.0
89	5.0
90	5.0
91	5.5
92	6.0
93	3.5
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	279742.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.549544209481546
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.43889632836333	38.59180529302861
2	10.837108933642583	11.389702700228366
3	5.317305241791838	8.382659006365445
4	3.131264032599642	6.581859908506174
5	2.1057696561251946	5.532861781976784
6	1.4630105177119166	4.612832152766331
7	1.0720448070219826	3.9434826186802754
8	0.769185983370953	3.233629827077235
9	0.5447130390299996	2.576197973338921
>10	1.2786041761361393	8.642557703246432
>50	0.02104864210322033	0.7578545385647748
>100	0.016295722918622194	2.03735848173487
>500	0.003394942274712957	1.3187525303839018
>1k	6.789884549425914E-4	0.5259310686650085
>5k	6.789884549425914E-4	1.8725144154368822
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5248	1.8760143274874705	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTC	1474	0.5269140851212903	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCG	947	0.3385262134395264	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	881	0.3149330454490209	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGC	720	0.2573800144418786	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTT	629	0.22485004039436338	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTT	519	0.1855280937435208	TruSeq Adapter, Index 23 (95% over 21bp)
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	500	0.17873612114019347	No Hit
GAACTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCT	467	0.1669395371449407	TruSeq Adapter, Index 23 (95% over 23bp)
CGTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTC	412	0.1472785638195194	TruSeq Adapter, Index 23 (95% over 22bp)
CGTTTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCT	407	0.1454912026081175	No Hit
GCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC	360	0.1286900072209393	Illumina PCR Primer Index 7 (95% over 23bp)
GAATCTATCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTC	284	0.10152211680762989	TruSeq Adapter, Index 23 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0983048666271064	0.0
2	0.0	0.0	0.0	0.502605972646224	0.0
3	0.0	0.0	0.0	0.7914435444087766	0.0
4	0.0	0.0	0.0	1.2436459308934662	0.0
5	0.0	0.0	0.0	2.5973933124092916	0.0
6	0.0	0.0	0.0	3.5983155907943747	0.0
7	0.0	0.0	0.0	4.280372629065353	0.0
8	0.0	0.0	0.0	5.216949903839967	0.0
9	0.0	0.0	0.0	5.706329403521816	0.0
10	0.0	0.0	0.0	6.842376189488887	0.0
11	0.0	0.0	0.0	7.55732067404966	0.0
12	0.0	0.0	0.0	8.479599059133058	0.0
13	0.0	0.0	0.0	8.889977193270942	0.0
14	0.0	0.0	0.0	9.126623817660558	0.0
15	0.0	0.0	0.0	9.388293499009801	0.0
16	0.0	0.0	0.0	9.73754387971774	0.0
17	0.0	0.0	0.0	10.15364156973211	0.0
18	0.0	0.0	0.0	10.636944041295193	0.0
19	0.0	0.0	0.0	10.931858641176513	0.0
20	0.0	0.0	0.0	11.270384854616038	0.0
21	0.0	0.0	0.0	11.63214676380379	0.0
22	0.0	0.0	0.0	12.022148980131693	0.0
23	0.0	0.0	0.0	12.431454697542735	0.0
24	0.0	0.0	0.0	12.753537187837365	0.0
25	0.0	0.0	0.0	13.047379370991843	0.0
26	0.0	0.0	0.0	13.336931887238956	0.0
27	0.0	0.0	0.0	13.653294821657099	0.0
28	0.0	0.0	0.0	13.9685853393484	0.0
29	0.0	0.0	0.0	14.295672441034954	0.0
30	0.0	0.0	0.0	14.67995510148637	0.0
31	0.0	0.0	0.0	15.031707787890271	0.0
32	0.0	0.0	0.0	15.385247835505574	0.0
33	0.0	0.0	0.0	15.731280966032987	0.0
34	0.0	0.0	0.0	16.079101457771802	0.0
35	0.0	0.0	0.0	16.446582922836043	0.0
36	0.0	0.0	0.0	16.7772447469454	0.0
37	0.0	0.0	0.0	17.11934568280773	0.0
38	0.0	0.0	0.0	17.49862373186722	0.0
39	0.0	0.0	0.0	17.85895575208585	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGT	20	7.0241233E-4	45.000004	8
TACGCGG	20	7.0241233E-4	45.000004	2
ATGCACG	20	7.0241233E-4	45.000004	1
AAATTCG	35	1.2075179E-7	45.0	30
CGTACAT	35	1.2075179E-7	45.0	35
CGTTTTT	1900	0.0	43.934208	1
CGTTTTA	355	0.0	43.09859	1
AGGCGAT	70	0.0	41.785713	7
GGCCGGT	45	1.9193976E-8	40.0	9
GCGATGA	45	1.9193976E-8	40.0	9
CGGTTTT	210	0.0	39.642857	1
CTCGTCC	40	3.4457298E-7	39.375004	37
TTCGTAC	40	3.4457298E-7	39.375004	33
CCTCGTC	40	3.4457298E-7	39.375004	36
TCGTCCC	40	3.4457298E-7	39.375004	38
CGTCCCC	40	3.4457298E-7	39.375004	39
TGCACGG	40	3.4457298E-7	39.375004	2
ATGGGTC	35	6.23135E-6	38.571426	5
CTGTATC	35	6.23135E-6	38.571426	3
CGCATGG	35	6.23135E-6	38.571426	2
>>END_MODULE