FastQCFastQC Report
Sat 18 Jun 2016
SRR3551163_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551163_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences260890
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42381.6244394189121851No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7590.29092721070182836No Hit
GAATCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTC3970.15217141323929625No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3820.14642186362068305No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT3020.11575759898807926No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTT2810.10770822952202078No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGGGC207.023252E-445.04
GTATACG207.023252E-445.01
TTACGGG453.8198777E-1045.03
TTAATCG302.1590295E-644.99999620
CGTTTTA2050.042.804881
CGTTTTT14500.042.6724131
CGAATAT700.041.78571314
ACGGGAC403.4445293E-739.3755
GCTACGA750.039.010
AACGTCT356.229735E-638.57142633
ATAGCGG356.229735E-638.5714262
TAACGTC356.229735E-638.57142632
ACGTCAT356.229735E-638.57142635
AGCTAAT356.229735E-638.57142640
TAATCGT356.229735E-638.57142621
TGCGACG301.13750415E-437.4999961
GGATGAC1650.036.818188
GCATATC552.732122E-936.8181828
TACGAAT800.036.562512
AGATCAC250.0021041336.037
Produced by FastQC (version 0.11.5)