##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551163_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	260890
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.929878492851394	33.0	31.0	34.0	30.0	34.0
2	32.04989842462341	33.0	31.0	34.0	30.0	34.0
3	31.56870328490935	33.0	31.0	34.0	28.0	34.0
4	35.51648970830618	37.0	35.0	37.0	33.0	37.0
5	35.72805396910575	37.0	35.0	37.0	35.0	37.0
6	35.731499865843844	37.0	35.0	37.0	35.0	37.0
7	26.771443903560886	35.0	10.0	37.0	0.0	37.0
8	31.11804208670321	35.0	19.0	37.0	17.0	37.0
9	35.892560082793516	39.0	32.0	39.0	32.0	39.0
10	36.713879412779335	38.0	35.0	39.0	33.0	39.0
11	37.05941584575875	39.0	37.0	39.0	34.0	39.0
12	37.11265284219403	39.0	37.0	39.0	34.0	39.0
13	37.106401165242055	39.0	37.0	39.0	34.0	39.0
14	38.296289623979455	40.0	38.0	41.0	34.0	41.0
15	38.37582889340335	40.0	38.0	41.0	34.0	41.0
16	38.32640959791483	40.0	38.0	41.0	34.0	41.0
17	38.212434359308524	40.0	37.0	41.0	33.0	41.0
18	38.02933420215417	40.0	37.0	41.0	33.0	41.0
19	37.85270420483729	40.0	37.0	41.0	33.0	41.0
20	37.79231093564337	40.0	36.0	41.0	33.0	41.0
21	37.72568898769596	40.0	36.0	41.0	33.0	41.0
22	37.70576488175093	39.0	35.0	41.0	33.0	41.0
23	37.64648319214995	39.0	35.0	41.0	33.0	41.0
24	37.57117175821227	39.0	35.0	41.0	33.0	41.0
25	37.441185940434664	39.0	35.0	41.0	33.0	41.0
26	37.304607305761046	39.0	35.0	41.0	33.0	41.0
27	37.15687071179424	39.0	35.0	41.0	32.0	41.0
28	37.04545210625168	39.0	35.0	41.0	32.0	41.0
29	36.939361416689025	39.0	35.0	41.0	32.0	41.0
30	36.78995362029974	39.0	35.0	41.0	31.0	41.0
31	36.60999655027023	39.0	35.0	41.0	31.0	41.0
32	36.382410211200124	39.0	35.0	41.0	30.0	41.0
33	36.150101575376596	39.0	35.0	41.0	30.0	41.0
34	35.838633140404	39.0	35.0	40.0	27.0	41.0
35	35.57577906397332	39.0	35.0	40.0	26.0	41.0
36	35.367725861474185	38.0	35.0	40.0	25.0	41.0
37	35.18503200582621	38.0	34.0	40.0	24.0	41.0
38	34.98147495112883	38.0	34.0	40.0	23.0	41.0
39	34.892625244355855	38.0	34.0	40.0	23.0	41.0
40	34.6499367549542	38.0	34.0	40.0	22.0	41.0
41	34.51606807466749	38.0	34.0	40.0	22.0	41.0
42	34.393617999923336	38.0	33.0	40.0	21.0	41.0
43	34.35815861090881	38.0	33.0	40.0	21.0	41.0
44	34.16533021579976	38.0	33.0	40.0	20.0	41.0
45	34.08021771627889	37.0	33.0	40.0	20.0	41.0
46	33.99248725516501	37.0	33.0	40.0	20.0	41.0
47	33.84423320173253	37.0	33.0	40.0	20.0	41.0
48	33.7314155391161	37.0	33.0	40.0	20.0	41.0
49	33.56398482118901	37.0	33.0	40.0	19.0	41.0
50	33.37800605619226	36.0	32.0	40.0	18.0	41.0
51	31.365414542527503	35.0	28.0	39.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	10.0
9	22.0
10	28.0
11	23.0
12	14.0
13	16.0
14	15.0
15	21.0
16	37.0
17	76.0
18	164.0
19	285.0
20	480.0
21	767.0
22	1221.0
23	1781.0
24	2615.0
25	3690.0
26	4733.0
27	5338.0
28	5485.0
29	5373.0
30	5951.0
31	7123.0
32	8945.0
33	12041.0
34	17878.0
35	22084.0
36	24659.0
37	37314.0
38	45133.0
39	47559.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.428149794932736	19.1670819119169	21.191689984284565	19.213078308865803
2	36.62424776725823	23.491509831729847	23.343171451569628	16.541070949442293
3	31.02380313542106	22.94798574111695	30.392502587297326	15.635708536164666
4	27.07577906397332	24.515696270458815	30.20660048296217	18.201924182605698
5	23.868680286710877	27.871516731189388	29.270573805051935	18.989229177047797
6	25.209858561079386	32.52788531565027	28.66380466863429	13.598451454636054
7	56.662961401356895	27.968875771397904	11.00157154356242	4.366591283682778
8	76.78600176319522	5.817011000804937	13.43171451569627	3.965272720303576
9	70.19855111349611	7.955076852313235	15.620376403848365	6.22599563034229
10	40.71141093947641	24.36965771014604	21.952163747173138	12.966767603204415
11	29.608264019318487	25.064586607382424	27.798305799379047	17.528843573920042
12	28.18314232051823	21.441603741040286	31.88738548813676	18.487868450304727
13	24.839970868948598	21.34232818429223	33.150369887692136	20.66733105906704
14	19.949787266664114	24.276131703016596	33.52102418643873	22.25305684388056
15	19.275556748054736	24.757177354440568	35.73843382268389	20.228832074820804
16	23.388401241902717	24.854919697956994	32.855992947219136	18.900686112921157
17	23.649814097895668	24.3320939859711	30.853233163402198	21.164858752731035
18	23.330905745716585	24.943846065391543	31.270267162405613	20.45498102648626
19	25.174211353443983	25.99754685882939	29.15634941929549	19.67189236843114
20	25.740733642531332	25.263137720878532	30.302426309939058	18.693702326651078
21	25.83234313312124	25.002108168193494	30.743991720648552	18.421556978036723
22	23.913526773736056	23.61340028364445	31.058683736440646	21.41438920617885
23	23.487293495342865	24.09942887807122	31.535129748169727	20.87814787841619
24	22.86595883322473	23.864080647015985	31.722948369044424	21.547012150714863
25	23.48154394572425	25.062286787534976	29.691057533826516	21.765111732914256
26	22.125416842347352	25.710835984514546	29.96243627582506	22.201310897313046
27	22.11430104641803	25.78059718655372	30.69377898731266	21.41132277971559
28	21.710682663191385	25.8507416919008	30.823718808693318	21.614856836214496
29	23.04917781440454	24.99290888880371	30.999655027022882	20.958258269768866
30	23.35812028057802	24.29912990149105	31.40097359040209	20.941776227528845
31	23.29449193146537	25.060753574303344	30.09505922036107	21.549695273870213
32	24.7334125493503	25.9929472191345	28.86503890528575	20.408601326229444
33	24.10556173099774	25.18992678906819	29.74318678370194	20.96132469623213
34	23.09095787496646	24.80509026792901	30.814902832611445	21.28904902449308
35	22.995132047989575	25.41224270765457	29.957070029514355	21.635555214841503
36	24.386906359001877	26.561002721453487	28.28395109049791	20.768139829046724
37	23.877879566100653	26.6480125723485	28.63160719077006	20.84250067078079
38	23.262677756909042	26.242094369274405	28.50588370577638	21.98934416804017
39	23.47119475641075	25.142397178887656	28.562229292038786	22.82417877266281
40	24.762160297443366	24.426386599716356	29.974318678370192	20.837134424470083
41	22.613745256621563	25.51458469086588	29.803748706351335	22.067921346161217
42	23.163402200160988	25.330599103070263	29.1824140442332	22.32358465253555
43	22.793897811338113	25.642991299014913	29.18088083100157	22.382230058645405
44	22.765150063245045	25.44405688221089	28.742765150063242	23.04802790448082
45	22.204377323776303	25.140480662348114	28.547663766338303	24.107478247537276
46	22.271072099352217	26.271992027291198	28.336080340373336	23.12085553298325
47	22.086703208248686	25.748783011997396	29.91107363256545	22.25344014718847
48	22.15493119705623	24.693549005327917	30.553106673310594	22.598413124305264
49	21.92418260569589	25.18724366591284	29.988500900762773	22.900072827628502
50	20.791521330829084	25.079918739698726	31.032235808195026	23.096324121277167
51	21.196289623979457	24.265015907087278	30.041396757254013	24.497297711679252
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	127.0
1	348.0
2	569.0
3	4097.5
4	7626.0
5	5143.5
6	2661.0
7	2236.0
8	1811.0
9	1720.5
10	1630.0
11	1614.5
12	1599.0
13	1505.0
14	1411.0
15	1340.5
16	1270.0
17	1236.5
18	1203.0
19	1157.5
20	1112.0
21	1177.0
22	1242.0
23	1310.5
24	1379.0
25	1625.0
26	2093.5
27	2316.0
28	2905.0
29	3494.0
30	4063.5
31	4633.0
32	5077.5
33	5522.0
34	6390.5
35	7259.0
36	7586.0
37	7913.0
38	8420.5
39	8928.0
40	9694.5
41	10461.0
42	11346.5
43	12232.0
44	12901.5
45	13571.0
46	14256.5
47	14942.0
48	16678.0
49	18414.0
50	18158.0
51	17902.0
52	17525.5
53	17149.0
54	16024.5
55	14900.0
56	14470.0
57	14040.0
58	13827.0
59	13614.0
60	13165.0
61	12716.0
62	11795.5
63	10875.0
64	9301.5
65	7728.0
66	6641.0
67	5554.0
68	4826.0
69	4098.0
70	3367.0
71	2636.0
72	2194.5
73	1753.0
74	1456.0
75	917.0
76	675.0
77	542.0
78	409.0
79	310.5
80	212.0
81	170.0
82	128.0
83	98.5
84	69.0
85	51.5
86	34.0
87	25.0
88	16.0
89	13.5
90	11.0
91	9.5
92	8.0
93	6.5
94	5.0
95	3.0
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	260890.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.37986219343814
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.07697649457506	34.110115800414825
2	11.09362905330817	10.95603747754958
3	6.2091535566987	9.198214409030344
4	4.127267038100063	8.152155103076028
5	2.906559683911948	7.1762758324287566
6	2.0337921920920783	6.025702690535837
7	1.4631062817210783	5.057359059601836
8	1.0389133881942478	4.104111995195988
9	0.6683316515127988	2.970191236609344
>10	1.3474626178840532	8.429098701854908
>50	0.020884825201064074	0.7272502817964324
>100	0.012376192711741674	1.184837381372129
>500	7.735120444838546E-4	0.28990701884637193
>1k	7.735120444838546E-4	1.6187430116876473
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4238	1.6244394189121851	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	759	0.29092721070182836	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTC	397	0.15217141323929625	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	382	0.14642186362068305	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	302	0.11575759898807926	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTT	281	0.10770822952202078	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.8330330790754726E-4	0.0	0.0	0.05557897964659435	0.0
2	3.8330330790754726E-4	0.0	0.0	0.2215493119705623	0.0
3	3.8330330790754726E-4	0.0	0.0	0.3875196442945303	0.0
4	3.8330330790754726E-4	0.0	0.0	0.6113687761125378	0.0
5	3.8330330790754726E-4	0.0	0.0	1.2580014565525701	0.0
6	3.8330330790754726E-4	0.0	0.0	1.7877266280808004	0.0
7	3.8330330790754726E-4	0.0	0.0	2.2013108973130437	0.0
8	3.8330330790754726E-4	0.0	0.0	2.7597838169343403	0.0
9	3.8330330790754726E-4	0.0	0.0	3.1101230403618385	0.0
10	3.8330330790754726E-4	0.0	0.0	3.728391276016712	0.0
11	3.8330330790754726E-4	0.0	0.0	4.230518609375599	0.0
12	3.8330330790754726E-4	0.0	0.0	4.705814711180958	0.0
13	3.8330330790754726E-4	0.0	0.0	4.927747326459428	0.0
14	3.8330330790754726E-4	0.0	0.0	5.06650312392196	0.0
15	3.8330330790754726E-4	0.0	0.0	5.203725708152861	0.0
16	3.8330330790754726E-4	0.0	0.0	5.415692437425735	0.0
17	3.8330330790754726E-4	0.0	0.0	5.654107094944229	0.0
18	3.8330330790754726E-4	0.0	0.0	5.927019050174403	0.0
19	3.8330330790754726E-4	0.0	0.0	6.091072865958833	0.0
20	3.8330330790754726E-4	0.0	0.0	6.268542297520027	0.0
21	3.8330330790754726E-4	0.0	0.0	6.462110468013339	0.0
22	3.8330330790754726E-4	0.0	0.0	6.684426386599716	0.0
23	3.8330330790754726E-4	0.0	0.0	6.89332668940933	0.0
24	3.8330330790754726E-4	0.0	0.0	7.077312277204952	0.0
25	3.8330330790754726E-4	0.0	0.0	7.236383149986584	0.0
26	3.8330330790754726E-4	0.0	0.0	7.407336425313351	0.0
27	3.8330330790754726E-4	0.0	0.0	7.5775230940243015	0.0
28	3.8330330790754726E-4	0.0	0.0	7.7442600329640845	0.0
29	3.8330330790754726E-4	0.0	0.0	7.9244125876806315	0.0
30	3.8330330790754726E-4	0.0	0.0	8.159760818735865	0.0
31	3.8330330790754726E-4	0.0	0.0	8.351795775997546	0.0
32	3.8330330790754726E-4	0.0	0.0	8.542680823335505	0.0
33	3.8330330790754726E-4	0.0	0.0	8.753114339376749	0.0
34	3.8330330790754726E-4	0.0	0.0	8.965081068649623	0.0
35	3.8330330790754726E-4	0.0	0.0	9.180880831001572	0.0
36	3.8330330790754726E-4	0.0	0.0	9.408946299206562	0.0
37	3.8330330790754726E-4	0.0	0.0	9.617846602016176	0.0
38	3.8330330790754726E-4	0.0	0.0	9.870826785235156	0.0
39	3.8330330790754726E-4	0.0	0.0	10.220399402046839	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATGGGC	20	7.023252E-4	45.0	4
GTATACG	20	7.023252E-4	45.0	1
TTACGGG	45	3.8198777E-10	45.0	3
TTAATCG	30	2.1590295E-6	44.999996	20
CGTTTTA	205	0.0	42.80488	1
CGTTTTT	1450	0.0	42.672413	1
CGAATAT	70	0.0	41.785713	14
ACGGGAC	40	3.4445293E-7	39.375	5
GCTACGA	75	0.0	39.0	10
AACGTCT	35	6.229735E-6	38.571426	33
ATAGCGG	35	6.229735E-6	38.571426	2
TAACGTC	35	6.229735E-6	38.571426	32
ACGTCAT	35	6.229735E-6	38.571426	35
AGCTAAT	35	6.229735E-6	38.571426	40
TAATCGT	35	6.229735E-6	38.571426	21
TGCGACG	30	1.13750415E-4	37.499996	1
GGATGAC	165	0.0	36.81818	8
GCATATC	55	2.732122E-9	36.81818	28
TACGAAT	80	0.0	36.5625	12
AGATCAC	25	0.00210413	36.0	37
>>END_MODULE