FastQCFastQC Report
Sat 18 Jun 2016
SRR3551161_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551161_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112313
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCG6970.6205871092393579No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGC6390.568945714209397No Hit
GAATCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTC4350.38731046272470687No Hit
GCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC2160.19231967804261305TruSeq Adapter, Index 20 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2070.18430635812417084No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCG1440.12821311869507537No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGC1350.12019979877663316No Hit
CTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGCT1290.11485758549767168TruSeq Adapter, Index 22 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCT1170.10417315893974874No Hit
CCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC1150.10239242118009492TruSeq Adapter, Index 20 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACCTC207.0061E-445.037
TGAAACG351.1988777E-745.04
CGACACC207.0061E-445.035
TGCATTG253.868289E-545.039
AAACGGC351.1988777E-745.06
AACGGCT253.868289E-545.07
TACGAAT253.868289E-545.012
CCTCAAG253.868289E-545.024
GCCTCAA253.868289E-545.023
TGCTCCC253.868289E-545.041
TGGGCTC253.868289E-545.06
TGGGCGA207.0061E-445.06
TGGGAGT207.0061E-445.06
GACTATC207.0061E-445.03
TACGGCT800.042.18757
ACACCCA501.0622898E-940.530
CATACGA501.0622898E-940.518
ACATACG501.0622898E-940.517
GTCACTT403.4212098E-739.3754
GAATGAA1200.039.3749961
Produced by FastQC (version 0.11.5)