##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551161_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	112313
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.974161495107424	33.0	31.0	34.0	30.0	34.0
2	32.10058497235405	33.0	31.0	34.0	30.0	34.0
3	32.008093453117624	33.0	31.0	34.0	30.0	34.0
4	35.76358925502836	37.0	35.0	37.0	35.0	37.0
5	35.81694906199638	37.0	35.0	37.0	35.0	37.0
6	35.79597197118766	37.0	35.0	37.0	35.0	37.0
7	26.774362718474265	35.0	10.0	37.0	0.0	37.0
8	31.08791502319411	35.0	19.0	37.0	17.0	37.0
9	35.83925280243605	39.0	32.0	39.0	32.0	39.0
10	36.65801821694728	38.0	35.0	39.0	33.0	39.0
11	37.02040725472563	39.0	37.0	39.0	34.0	39.0
12	37.02765485740743	39.0	37.0	39.0	34.0	39.0
13	36.92445220054669	39.0	37.0	39.0	33.0	39.0
14	38.11563220642312	40.0	37.0	41.0	33.0	41.0
15	38.15398929776607	40.0	37.0	41.0	33.0	41.0
16	38.115097985095225	40.0	37.0	41.0	33.0	41.0
17	38.00135336069734	40.0	37.0	41.0	33.0	41.0
18	37.91342943381443	40.0	36.0	41.0	33.0	41.0
19	37.811909574136564	40.0	36.0	41.0	33.0	41.0
20	37.89320025286476	40.0	36.0	41.0	33.0	41.0
21	37.84150543570201	40.0	36.0	41.0	33.0	41.0
22	37.84455940095982	40.0	36.0	41.0	33.0	41.0
23	37.8076001887582	40.0	36.0	41.0	33.0	41.0
24	37.749263219751946	40.0	36.0	41.0	33.0	41.0
25	37.67289628092919	39.0	36.0	41.0	33.0	41.0
26	37.52061649141239	39.0	36.0	41.0	33.0	41.0
27	37.40036327050297	39.0	35.0	41.0	33.0	41.0
28	37.39121027841835	39.0	36.0	41.0	33.0	41.0
29	37.34978141444	39.0	36.0	41.0	32.0	41.0
30	37.25467221069689	39.0	35.0	41.0	32.0	41.0
31	37.08895675478351	39.0	35.0	41.0	32.0	41.0
32	36.99298389322696	39.0	35.0	41.0	31.0	41.0
33	36.898177414902996	39.0	35.0	41.0	31.0	41.0
34	36.67514891419515	39.0	35.0	41.0	30.0	41.0
35	36.65685183371471	39.0	35.0	41.0	31.0	41.0
36	36.44036754427359	39.0	35.0	41.0	30.0	41.0
37	36.36724154817341	39.0	35.0	40.0	30.0	41.0
38	36.24164611398503	39.0	35.0	40.0	30.0	41.0
39	36.0725472563283	39.0	35.0	40.0	30.0	41.0
40	35.84528059975248	38.0	35.0	40.0	28.0	41.0
41	35.7535993161967	38.0	35.0	40.0	28.0	41.0
42	35.72135015536937	38.0	35.0	40.0	28.0	41.0
43	35.687311353093584	38.0	35.0	40.0	28.0	41.0
44	35.42453678559027	38.0	34.0	40.0	27.0	41.0
45	35.28165929144445	38.0	34.0	40.0	26.0	41.0
46	35.008707807644704	38.0	34.0	40.0	24.0	41.0
47	34.798064338055255	38.0	34.0	40.0	24.0	41.0
48	34.738721252214795	38.0	34.0	40.0	23.0	41.0
49	34.58051160595835	37.0	34.0	40.0	23.0	41.0
50	34.40200154924185	37.0	33.0	40.0	23.0	41.0
51	32.176114964429765	35.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	12.0
10	13.0
11	7.0
12	7.0
13	3.0
14	8.0
15	7.0
16	9.0
17	17.0
18	31.0
19	82.0
20	132.0
21	191.0
22	298.0
23	435.0
24	664.0
25	850.0
26	1165.0
27	1385.0
28	1767.0
29	1936.0
30	2476.0
31	3163.0
32	3936.0
33	5386.0
34	8380.0
35	10413.0
36	10914.0
37	16602.0
38	20658.0
39	21360.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.15149626490255	19.722561057045933	22.980420788332605	12.14552188971891
2	31.658846260005518	26.770721109755772	24.16728250514188	17.40315012509683
3	32.75845182659175	25.850079688014745	25.44941369209263	15.942054793300864
4	29.258411759992164	24.279468984000072	27.68245884269853	18.779660413309234
5	26.608673973627273	28.628030593074712	23.709632900910847	21.05366253238717
6	25.836724154817343	36.42499087371898	23.953593973983423	13.784690997480256
7	58.063625760152426	28.504269318778768	8.72561502230374	4.706489898765058
8	77.25285585818204	7.695458228344003	9.953433707585052	5.0982522058889
9	70.54926856196522	8.640139609840357	11.677187858929955	9.133403969264467
10	42.191019739478065	24.570619607703474	18.765414511232002	14.47294614158646
11	32.830571705857736	24.916973101956142	24.286591935038686	17.965863257147436
12	32.031020451773166	21.43385004407326	27.98785536847916	18.54727413567441
13	25.260655489569327	24.664108340085296	29.48901729986734	20.58621887047804
14	20.487387924817252	28.283457836581693	28.480229359023447	22.748924879577608
15	19.889950406453394	25.591872712864937	35.38593039096097	19.132246489720693
16	22.19422506744544	24.96594339034662	31.316054241272155	21.52377730093578
17	22.052656415552963	24.460213866604935	29.082118721786436	24.405010996055665
18	22.842413611959433	25.887475180967474	29.137321592335702	22.132789614737387
19	24.49048640851905	27.017353289467827	27.012011076188863	21.480149225824256
20	27.232822558385937	25.829601203778722	27.93443323568955	19.00314300214579
21	24.915192364196486	27.68245884269853	27.856971143144605	19.54537764996038
22	22.098955597303963	25.224150365496424	28.106274429496143	24.570619607703474
23	23.78442388681631	26.931877877004446	28.474887145744482	20.808811090434766
24	24.540347065789355	23.833394175206788	28.01278569711431	23.613473061889543
25	21.869240426308618	27.327201659647592	27.361926045960843	23.441631868082947
26	20.53991968872704	28.90849679022019	27.04851620026177	23.503067320791004
27	21.403577502159145	29.21300294712099	26.670999795215156	22.712419755504705
28	19.35483870967742	29.430252953798757	29.165813396490165	22.049094940033655
29	23.14691976886024	27.779509050599664	27.000436280751117	22.073134899788982
30	22.270796791110556	28.29325189425979	27.76259204188295	21.673359272746698
31	24.102285576914515	27.735880975488147	25.977402437829994	22.184431009767348
32	27.156250834720826	28.270992672264118	24.1414618076269	20.431294685388156
33	25.095046877921522	26.199104288906895	25.136003846393564	23.569844986778023
34	23.058773249757376	27.060981364579344	28.493584892220845	21.386660493442434
35	22.066902317630195	27.035160667064368	25.9346647315983	24.963272283707138
36	22.22983982263852	30.31260851370723	25.465440331929518	21.992111331724733
37	23.303624691709775	30.071318547274135	27.245287722703516	19.37976903831257
38	23.110414644787337	28.328866649452866	24.560825550025374	23.99989315573442
39	25.379964919466136	25.20367188126041	26.877565375335	22.53879782393846
40	25.648856321173863	24.007016106773037	27.724306180050394	22.619821392002706
41	19.41271268686617	26.63538504002208	28.130314389251467	25.821587883860282
42	21.030512941511667	25.56249053983065	27.56849162608069	25.838504892576996
43	23.177192310774352	25.55180611327273	25.547354268873594	25.72364730707932
44	22.358052941333597	26.825033611425216	26.654973155378276	24.16194029186292
45	22.30819228406329	25.878571492169204	26.27834711921149	25.53488910455602
46	23.80134089553302	28.046619714547738	25.0941565090417	23.057882880877546
47	20.44465021858556	25.714743618281055	30.336648473462557	23.50395768967083
48	22.24675683135523	24.935670848432505	28.626249855315056	24.19132246489721
49	21.57808980260522	24.083587830438148	29.78640050572952	24.551921861227104
50	20.206031358791947	24.330220010150207	29.411555207322394	26.052193423735453
51	20.163293652560256	24.80122514757864	27.43493629410665	27.600544905754454
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	52.0
1	174.0
2	296.0
3	431.5
4	567.0
5	408.0
6	249.0
7	236.5
8	224.0
9	226.0
10	228.0
11	243.0
12	258.0
13	239.0
14	220.0
15	248.0
16	276.0
17	258.0
18	240.0
19	257.0
20	274.0
21	348.0
22	422.0
23	496.0
24	570.0
25	737.5
26	917.0
27	929.0
28	1152.5
29	1376.0
30	1727.5
31	2079.0
32	2384.5
33	2690.0
34	2958.5
35	3227.0
36	3343.0
37	3459.0
38	3721.5
39	3984.0
40	4515.0
41	5046.0
42	5667.5
43	6289.0
44	7010.0
45	7731.0
46	9632.5
47	11534.0
48	10595.5
49	9657.0
50	9327.5
51	8998.0
52	8080.5
53	7163.0
54	6483.0
55	5803.0
56	5721.0
57	5639.0
58	5476.0
59	5313.0
60	5015.0
61	4717.0
62	4339.5
63	3962.0
64	3329.0
65	2696.0
66	2288.5
67	1881.0
68	1509.5
69	1138.0
70	984.5
71	831.0
72	716.0
73	601.0
74	474.5
75	283.0
76	218.0
77	170.5
78	123.0
79	82.0
80	41.0
81	34.0
82	27.0
83	21.5
84	16.0
85	12.0
86	8.0
87	6.5
88	5.0
89	4.0
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	112313.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.282807867299425
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.57505736120395	34.7813699215585
2	10.388249534787086	10.239242118009491
3	5.728893786923452	8.470079153793417
4	3.716283355314267	7.325955143215834
5	2.567252624162165	6.326070891170212
6	1.9150512185868367	5.662746075699162
7	1.365828982312876	4.71183211204402
8	1.0677313869668117	4.209664063821641
9	0.7967335730158444	3.5338740840330147
>10	1.8445917869595854	11.597054659745533
>50	0.0144532167440516	0.5208657946987436
>100	0.016259868837058048	1.431713158761675
>500	0.0036133041860129	1.1895328234487548
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCG	697	0.6205871092393579	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGC	639	0.568945714209397	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTC	435	0.38731046272470687	No Hit
GCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC	216	0.19231967804261305	TruSeq Adapter, Index 20 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	207	0.18430635812417084	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCG	144	0.12821311869507537	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGC	135	0.12019979877663316	No Hit
CTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGCT	129	0.11485758549767168	TruSeq Adapter, Index 22 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCT	117	0.10417315893974874	No Hit
CCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC	115	0.10239242118009492	TruSeq Adapter, Index 20 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.17095082492676716	0.0
2	0.0	0.0	0.0	0.8342756403978168	0.0
3	0.0	0.0	0.0	1.0631004425133332	0.0
4	0.0	0.0	0.0	1.4094539367660022	0.0
5	0.0	0.0	0.0	2.7280902477896594	0.0
6	0.0	0.0	0.0	3.195533909698788	0.0
7	8.903688798269123E-4	0.0	0.0	3.69681158904134	0.0
8	8.903688798269123E-4	0.0	0.0	4.487459154327638	0.0
9	8.903688798269123E-4	0.0	0.0	4.77237719587225	0.0
10	8.903688798269123E-4	0.0	0.0	6.417778885792384	0.0
11	8.903688798269123E-4	0.0	0.0	7.455058630790736	0.0
12	8.903688798269123E-4	0.0	0.0	9.087995156393294	0.0
13	8.903688798269123E-4	0.0	0.0	9.509139636551422	0.0
14	8.903688798269123E-4	0.0	0.0	9.705020790113345	0.0
15	8.903688798269123E-4	0.0	0.0	10.105686786035454	0.0
16	8.903688798269123E-4	0.0	0.0	10.731616108553775	0.0
17	8.903688798269123E-4	0.0	0.0	11.405625350582746	0.0
18	8.903688798269123E-4	0.0	0.0	12.150864102997872	0.0
19	8.903688798269123E-4	0.0	0.0	12.614746289387693	0.0
20	8.903688798269123E-4	0.0	0.0	13.037671507305477	0.0
21	8.903688798269123E-4	0.0	0.0	13.591480950557816	0.0
22	8.903688798269123E-4	0.0	0.0	14.095429736539849	0.0
23	8.903688798269123E-4	0.0	0.0	14.593145940363092	0.0
24	8.903688798269123E-4	0.0	0.0	15.000934887323819	0.0
25	8.903688798269123E-4	0.0	0.0	15.377560923490602	0.0
26	8.903688798269123E-4	0.0	0.0	15.71857220446431	0.0
27	8.903688798269123E-4	0.0	0.0	16.09341750287144	0.0
28	8.903688798269123E-4	0.0	0.0	16.478947227836493	0.0
29	8.903688798269123E-4	0.0	0.0	16.829752566488295	0.0
30	8.903688798269123E-4	0.0	0.0	17.23754151344902	0.0
31	8.903688798269123E-4	0.0	0.0	17.615057918495634	0.0
32	8.903688798269123E-4	0.0	0.0	17.975657314825533	0.0
33	8.903688798269123E-4	0.0	0.0	18.33358560451595	0.0
34	8.903688798269123E-4	0.0	0.0	18.685281312047582	0.0
35	8.903688798269123E-4	0.0	0.0	19.06101697933454	0.0
36	8.903688798269123E-4	0.0	0.0	19.40113789142842	0.0
37	8.903688798269123E-4	0.0	0.0	19.783106140874164	0.0
38	8.903688798269123E-4	0.0	0.0	20.14192479944441	0.0
39	8.903688798269123E-4	0.0	0.0	20.526564155529638	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACCTC	20	7.0061E-4	45.0	37
TGAAACG	35	1.1988777E-7	45.0	4
CGACACC	20	7.0061E-4	45.0	35
TGCATTG	25	3.868289E-5	45.0	39
AAACGGC	35	1.1988777E-7	45.0	6
AACGGCT	25	3.868289E-5	45.0	7
TACGAAT	25	3.868289E-5	45.0	12
CCTCAAG	25	3.868289E-5	45.0	24
GCCTCAA	25	3.868289E-5	45.0	23
TGCTCCC	25	3.868289E-5	45.0	41
TGGGCTC	25	3.868289E-5	45.0	6
TGGGCGA	20	7.0061E-4	45.0	6
TGGGAGT	20	7.0061E-4	45.0	6
GACTATC	20	7.0061E-4	45.0	3
TACGGCT	80	0.0	42.1875	7
ACACCCA	50	1.0622898E-9	40.5	30
CATACGA	50	1.0622898E-9	40.5	18
ACATACG	50	1.0622898E-9	40.5	17
GTCACTT	40	3.4212098E-7	39.375	4
GAATGAA	120	0.0	39.374996	1
>>END_MODULE