FastQCFastQC Report
Sat 18 Jun 2016
SRR3551152_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551152_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131981
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9980.7561694486327577No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCG5350.40536137777407355No Hit
GAATCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTC5240.39702684477311123No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGC4550.3447465923125298No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1740.13183715837885757No Hit
GCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC1630.12350262537789532TruSeq Adapter, Index 21 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTT1560.11819883165001023No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGCAT207.01058E-445.00000425
CGCATCA207.01058E-445.00000426
CAATTGG253.8719976E-545.0000042
TAACGTC406.7357178E-945.00000432
TACGGCT253.8719976E-545.0000047
GCGCATC207.01058E-445.00000425
ATCACGG207.01058E-445.0000042
ATGTAGG253.8719976E-545.0000042
GGCTTAA207.01058E-445.0000048
AACGGGC207.01058E-445.0000044
AAACGGC207.01058E-445.0000046
AACGGCT207.01058E-445.0000047
TACGAAA207.01058E-445.00000420
CGGGCAT207.01058E-445.0000046
GATATGG207.01058E-445.0000042
GCTAGTC207.01058E-445.00000437
CATACGA207.01058E-445.00000418
TAGATGG302.1508713E-645.0000042
ATAGTTA207.01058E-445.00000437
CGGTAGT253.8719976E-545.00000412