##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551152_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	131981
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.94936392359506	33.0	31.0	34.0	30.0	34.0
2	32.06001621445511	33.0	31.0	34.0	30.0	34.0
3	31.77277789984922	33.0	31.0	34.0	30.0	34.0
4	35.6215743175154	37.0	35.0	37.0	33.0	37.0
5	35.76980019851342	37.0	35.0	37.0	35.0	37.0
6	35.76940620240792	37.0	35.0	37.0	35.0	37.0
7	26.855107932202362	35.0	11.0	37.0	0.0	37.0
8	31.149127525931764	35.0	19.0	37.0	17.0	37.0
9	35.932520590084934	39.0	32.0	39.0	32.0	39.0
10	36.680325198324	38.0	35.0	39.0	33.0	39.0
11	37.018017744978444	39.0	37.0	39.0	34.0	39.0
12	37.09299065774619	39.0	37.0	39.0	34.0	39.0
13	37.04250611830491	39.0	37.0	39.0	33.0	39.0
14	38.284245459573725	40.0	37.0	41.0	34.0	41.0
15	38.29294368128746	40.0	37.0	41.0	34.0	41.0
16	38.234867139967115	40.0	37.0	41.0	34.0	41.0
17	38.10894750001894	40.0	37.0	41.0	33.0	41.0
18	37.98062599919685	40.0	37.0	41.0	33.0	41.0
19	37.82423985270607	40.0	37.0	41.0	33.0	41.0
20	37.85710822012259	40.0	36.0	41.0	33.0	41.0
21	37.81647358331881	40.0	36.0	41.0	33.0	41.0
22	37.792394359794216	40.0	36.0	41.0	33.0	41.0
23	37.76813329191323	39.0	36.0	41.0	33.0	41.0
24	37.6739757995469	39.0	36.0	41.0	33.0	41.0
25	37.59925292276919	39.0	35.0	41.0	33.0	41.0
26	37.448041763587185	39.0	35.0	41.0	33.0	41.0
27	37.3547859161546	39.0	35.0	41.0	33.0	41.0
28	37.31896257794683	39.0	35.0	41.0	33.0	41.0
29	37.26360612512407	39.0	35.0	41.0	32.0	41.0
30	37.16149294216592	39.0	35.0	41.0	32.0	41.0
31	37.03666436835605	39.0	35.0	41.0	32.0	41.0
32	36.883695380395665	39.0	35.0	41.0	31.0	41.0
33	36.70248747925838	39.0	35.0	41.0	31.0	41.0
34	36.48886582159553	39.0	35.0	41.0	30.0	41.0
35	36.27800971351937	39.0	35.0	41.0	30.0	41.0
36	36.088399087747476	39.0	35.0	41.0	29.0	41.0
37	36.012645759616916	39.0	35.0	40.0	29.0	41.0
38	35.83392306468355	39.0	35.0	40.0	28.0	41.0
39	35.73258272024003	39.0	35.0	40.0	27.0	41.0
40	35.469984315924265	38.0	35.0	40.0	26.0	41.0
41	35.36775748024337	38.0	35.0	40.0	26.0	41.0
42	35.28676097317038	38.0	34.0	40.0	25.0	41.0
43	35.25672634697418	38.0	34.0	40.0	25.0	41.0
44	35.061743735840764	38.0	34.0	40.0	24.0	41.0
45	34.93999136239307	38.0	34.0	40.0	24.0	41.0
46	34.71918685265303	38.0	34.0	40.0	23.0	41.0
47	34.50495147028739	38.0	33.0	40.0	23.0	41.0
48	34.43134996704071	37.0	33.0	40.0	23.0	41.0
49	34.224077708154965	37.0	33.0	40.0	22.0	41.0
50	34.04249096460854	37.0	33.0	40.0	22.0	41.0
51	31.830324061796773	35.0	29.0	39.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	8.0
10	9.0
11	7.0
12	8.0
13	1.0
14	10.0
15	13.0
16	13.0
17	25.0
18	48.0
19	98.0
20	173.0
21	289.0
22	418.0
23	654.0
24	908.0
25	1380.0
26	1757.0
27	2007.0
28	2269.0
29	2379.0
30	2960.0
31	3549.0
32	4535.0
33	6177.0
34	9506.0
35	11787.0
36	12749.0
37	19263.0
38	23902.0
39	25072.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.47718232169782	19.974844864033457	24.181510975064594	14.366461839204128
2	33.19796031246922	25.824171661072427	24.07846583977997	16.899402186678387
3	30.533940491434375	24.520196088830968	27.988119502049535	16.95774391768512
4	27.58881960282162	23.719323235920324	30.16267493048242	18.529182230775643
5	25.36804540047431	27.74641804502163	26.690963093172503	20.194573461331554
6	24.703555814852137	34.72696827573666	26.706874474356155	13.862601435055048
7	58.09775649525311	27.820671157212022	9.646085421386411	4.435486926148461
8	78.58403861161834	6.044051795334177	10.80988930224805	4.562020290799433
9	72.8551837006842	7.277562679476592	12.402542790250113	7.464710829589108
10	40.93846841590835	24.21409142225017	19.980148657761347	14.867291504080132
11	30.706692629999772	24.82099696168388	25.424871761844507	19.04743864647184
12	28.336654518453415	21.440964987384547	30.5506095574363	19.671770936725743
13	24.310317394170372	23.21318977731643	32.49255574666051	19.98393708185269
14	21.117433569983557	27.00464460793599	30.61122434289784	21.266697479182607
15	19.266409558951665	25.571862616588753	35.783180912404056	19.378546912055523
16	22.91238890446352	24.487615641645387	32.00536440851335	20.594631045377742
17	22.652503011797155	24.49822322910116	29.403474742576584	23.445799016525108
18	22.74190982035293	26.008289071911868	30.45514127033437	20.79465983740084
19	24.406543366090574	27.42364431243891	27.61912699555239	20.550685325918124
20	25.838567672619543	26.360612512407087	29.06327425917367	18.7375455557997
21	25.330918844379113	26.59549480607057	28.995082625529434	19.07850372402088
22	23.176820906039506	24.380782082269416	28.816269008417876	23.626128003273198
23	23.104083163485654	26.519726324243642	28.868549260878464	21.507641251392247
24	23.176063221221234	24.075435100506894	29.970980671460286	22.777521006811586
25	23.21243209249816	26.755366302725392	27.56760442791008	22.464597176866366
26	20.999992423151816	27.238011531962936	28.9761405050727	22.785855539812548
27	22.045597472363447	27.79112144929952	28.48137231874285	21.681908759594183
28	20.17941976496617	26.872807449557136	30.55212492707284	22.395647858403862
29	21.90012198725574	26.17952584084073	29.667149059334296	22.253203112569235
30	22.238807101022118	26.79173517400232	29.53455421613717	21.434903508838392
31	23.10862927239527	25.933278274903206	27.386517756343714	23.571574696357807
32	23.479894833347224	27.266803555057166	27.315295383426403	21.938006228169208
33	23.346542305331827	27.751721838749514	26.591706381979225	22.31002947393943
34	22.20546896901827	26.426531091596516	29.315583303657345	22.05241663572787
35	20.797690576673915	26.975852584841757	29.076154901084244	23.15030193740008
36	21.765254089603804	29.68836423424584	27.74944878429471	20.796932891855647
37	22.532031125692335	28.731408308771716	27.372879429614873	21.363681135921077
38	21.393988528651853	28.702616285677486	26.021927398640717	23.88146778702995
39	24.04437002295785	26.387889165864785	26.625044513983077	22.942696297194292
40	22.57824989960676	26.132549382108035	29.861874057629507	21.4273266606557
41	20.356718012441185	27.430463475803336	29.196626787189068	23.016191724566415
42	21.403080746471083	27.11223585213023	29.928550321637204	21.55613307976148
43	22.264568384843272	26.29090550912631	27.805517460846634	23.639008645183775
44	21.290185708548957	26.251505898576312	28.22072874125821	24.23757965161652
45	21.489456815753783	25.921155317810896	27.85931308294376	24.730074783491563
46	22.77524795235678	27.464559292625452	27.11753964585812	22.642653109159653
47	21.143952538622983	26.462899962873443	30.509694577249757	21.883452921253816
48	21.58947121176533	25.652177207325295	29.058728150264052	23.69962343064532
49	20.993930944605662	25.246815829551224	30.033110826558367	23.726142399284743
50	19.377031542418983	25.95297808017821	29.98689205264394	24.683098324758866
51	20.122593403595975	25.264242580371416	28.013880785870693	26.59928323016192
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	61.0
1	200.5
2	340.0
3	1146.5
4	1953.0
5	1368.0
6	783.0
7	662.5
8	542.0
9	553.0
10	564.0
11	543.0
12	522.0
13	493.0
14	464.0
15	470.0
16	476.0
17	482.5
18	489.0
19	482.0
20	475.0
21	521.5
22	568.0
23	618.0
24	668.0
25	831.0
26	1137.0
27	1280.0
28	1515.0
29	1750.0
30	2135.0
31	2520.0
32	2618.5
33	2717.0
34	3040.5
35	3364.0
36	3594.5
37	3825.0
38	4257.0
39	4689.0
40	5589.0
41	6489.0
42	7409.0
43	8329.0
44	10423.5
45	12518.0
46	11715.0
47	10912.0
48	10618.5
49	10325.0
50	9345.0
51	8365.0
52	7629.0
53	6893.0
54	6674.5
55	6456.0
56	6267.5
57	6079.0
58	5937.5
59	5796.0
60	5678.5
61	5561.0
62	5218.0
63	4875.0
64	4169.0
65	3463.0
66	2954.0
67	2445.0
68	1977.5
69	1510.0
70	1306.0
71	1102.0
72	939.5
73	777.0
74	628.5
75	377.5
76	275.0
77	205.0
78	135.0
79	104.5
80	74.0
81	55.0
82	36.0
83	29.5
84	23.0
85	15.5
86	8.0
87	6.5
88	5.0
89	5.0
90	5.0
91	3.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	131981.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.51361938460839
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.57159346684365	38.635106568369686
2	9.920932648467709	10.419681620839363
3	4.9734518381716395	7.835218705722793
4	3.40941882610954	7.161636902281389
5	2.2652507647024875	5.947825823413976
6	1.6029895538754544	5.050726998583129
7	1.174467593928551	4.31728809449845
8	0.906100305880995	3.806608526984945
9	0.594447971374156	2.809495306142551
>10	1.5481618283603624	10.677294459050923
>50	0.014428348819761067	0.5273486335154303
>100	0.014428348819761067	1.2532106894174162
>500	0.00432850464592832	1.5585576711799425
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	998	0.7561694486327577	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCG	535	0.40536137777407355	No Hit
GAATCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTC	524	0.39702684477311123	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGC	455	0.3447465923125298	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	174	0.13183715837885757	No Hit
GCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC	163	0.12350262537789532	TruSeq Adapter, Index 21 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTT	156	0.11819883165001023	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09546828710193134	0.0
2	0.0	0.0	0.0	0.5053757737856207	0.0
3	0.0	0.0	0.0	0.7167698380827543	0.0
4	0.0	0.0	0.0	1.0781854963972086	0.0
5	0.0	0.0	0.0	2.2238049416203847	0.0
6	0.0	0.0	0.0	2.7549419992271615	0.0
7	0.0	0.0	0.0	3.280017578287784	0.0
8	0.0	0.0	0.0	4.208939165485941	0.0
9	0.0	0.0	0.0	4.560504921162894	0.0
10	0.0	0.0	0.0	5.937218235958206	0.0
11	0.0	0.0	0.0	6.9419083049832935	0.0
12	0.0	0.0	0.0	8.192088255127633	0.0
13	0.0	0.0	0.0	8.575476773171896	0.0
14	0.0	0.0	0.0	8.752017335828642	0.0
15	0.0	0.0	0.0	9.047514414953667	0.0
16	0.0	0.0	0.0	9.493790772914283	0.0
17	0.0	0.0	0.0	10.03553541797683	0.0
18	0.0	0.0	0.0	10.640167902955728	0.0
19	0.0	0.0	0.0	11.000068191633645	0.0
20	0.0	0.0	0.0	11.382699024859638	0.0
21	0.0	0.0	0.0	11.8327638069116	0.0
22	0.0	0.0	0.0	12.301770709420296	0.0
23	0.0	0.0	0.0	12.794265841295339	0.0
24	0.0	0.0	0.0	13.194323425341526	0.0
25	0.0	0.0	0.0	13.515581788287708	0.0
26	0.0	0.0	0.0	13.861843750236776	0.0
27	0.0	0.0	0.0	14.217197930005076	0.0
28	0.0	0.0	0.0	14.584675066865685	0.0
29	0.0	0.0	0.0	14.958213682272449	0.0
30	0.0	0.0	0.0	15.390851713504217	0.0
31	0.0	0.0	0.0	15.78409013418598	0.0
32	0.0	0.0	0.0	16.193239936051402	0.0
33	0.0	0.0	0.0	16.608451216462974	0.0
34	0.0	0.0	0.0	16.972897614050506	0.0
35	0.0	0.0	0.0	17.361589925822656	0.0
36	0.0	0.0	0.0	17.7775588910525	0.0
37	0.0	0.0	0.0	18.183677953644843	0.0
38	0.0	0.0	0.0	18.614042930421803	0.0
39	0.0	0.0	0.0	19.023192732287225	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGCAT	20	7.01058E-4	45.000004	25
CGCATCA	20	7.01058E-4	45.000004	26
CAATTGG	25	3.8719976E-5	45.000004	2
TAACGTC	40	6.7357178E-9	45.000004	32
TACGGCT	25	3.8719976E-5	45.000004	7
GCGCATC	20	7.01058E-4	45.000004	25
ATCACGG	20	7.01058E-4	45.000004	2
ATGTAGG	25	3.8719976E-5	45.000004	2
GGCTTAA	20	7.01058E-4	45.000004	8
AACGGGC	20	7.01058E-4	45.000004	4
AAACGGC	20	7.01058E-4	45.000004	6
AACGGCT	20	7.01058E-4	45.000004	7
TACGAAA	20	7.01058E-4	45.000004	20
CGGGCAT	20	7.01058E-4	45.000004	6
GATATGG	20	7.01058E-4	45.000004	2
GCTAGTC	20	7.01058E-4	45.000004	37
CATACGA	20	7.01058E-4	45.000004	18
TAGATGG	30	2.1508713E-6	45.000004	2
ATAGTTA	20	7.01058E-4	45.000004	37
CGGTAGT	25	3.8719976E-5	45.000004	12
>>END_MODULE