##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551150_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	152468
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.03053755542147	33.0	31.0	34.0	30.0	34.0
2	32.145007476978776	33.0	31.0	34.0	30.0	34.0
3	32.04724925886087	33.0	31.0	34.0	30.0	34.0
4	35.796908203688645	37.0	35.0	37.0	35.0	37.0
5	35.85812104835113	37.0	35.0	37.0	35.0	37.0
6	35.83295511189233	37.0	35.0	37.0	35.0	37.0
7	26.760795707951832	35.0	10.0	37.0	0.0	37.0
8	31.11571608468662	35.0	19.0	37.0	17.0	37.0
9	35.91257837710208	39.0	32.0	39.0	32.0	39.0
10	36.687783666080755	38.0	35.0	39.0	33.0	39.0
11	37.07941994385707	39.0	37.0	39.0	34.0	39.0
12	37.09990293045098	39.0	37.0	39.0	34.0	39.0
13	37.07581918828869	39.0	37.0	39.0	33.0	39.0
14	38.26301256657135	40.0	38.0	41.0	33.0	41.0
15	38.34249809796154	40.0	38.0	41.0	34.0	41.0
16	38.298383923183884	40.0	37.0	41.0	34.0	41.0
17	38.20739433848414	40.0	37.0	41.0	34.0	41.0
18	38.11589317102605	40.0	37.0	41.0	33.0	41.0
19	38.02881916205368	40.0	37.0	41.0	34.0	41.0
20	38.067673216674976	40.0	37.0	41.0	34.0	41.0
21	37.99412991578561	40.0	37.0	41.0	33.0	41.0
22	38.001797098407536	40.0	37.0	41.0	33.0	41.0
23	37.96470734842721	40.0	37.0	41.0	33.0	41.0
24	37.93571765878742	40.0	36.0	41.0	33.0	41.0
25	37.81308864810977	40.0	36.0	41.0	33.0	41.0
26	37.69261746727182	40.0	36.0	41.0	33.0	41.0
27	37.559428863761575	40.0	36.0	41.0	33.0	41.0
28	37.472486029855446	40.0	36.0	41.0	33.0	41.0
29	37.43250386966445	40.0	36.0	41.0	33.0	41.0
30	37.351450796232655	40.0	36.0	41.0	32.0	41.0
31	37.22386992680431	40.0	36.0	41.0	32.0	41.0
32	37.096859668914135	39.0	35.0	41.0	31.0	41.0
33	36.97638848807619	39.0	35.0	41.0	31.0	41.0
34	36.836201694781856	39.0	35.0	41.0	31.0	41.0
35	36.74794711021329	39.0	35.0	41.0	30.0	41.0
36	36.603805388671724	39.0	35.0	41.0	30.0	41.0
37	36.53852611695569	39.0	35.0	41.0	30.0	41.0
38	36.41930765800037	39.0	35.0	41.0	30.0	41.0
39	36.36340740352074	39.0	35.0	41.0	30.0	41.0
40	36.17350526012016	39.0	35.0	41.0	29.0	41.0
41	36.07252669412598	39.0	35.0	40.0	29.0	41.0
42	36.03807356297715	39.0	35.0	40.0	29.0	41.0
43	35.97526038250649	39.0	35.0	40.0	29.0	41.0
44	35.79712464254794	38.0	35.0	40.0	28.0	41.0
45	35.66034184222263	38.0	35.0	40.0	27.0	41.0
46	35.49292310517617	38.0	34.0	40.0	27.0	41.0
47	35.39197733294856	38.0	34.0	40.0	26.0	41.0
48	35.32279560301178	38.0	34.0	40.0	26.0	41.0
49	35.14748012697746	38.0	34.0	40.0	26.0	41.0
50	34.94257155599863	38.0	34.0	40.0	25.0	41.0
51	32.79280242411522	35.0	31.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	11.0
10	15.0
11	8.0
12	6.0
13	3.0
14	7.0
15	17.0
16	16.0
17	34.0
18	60.0
19	144.0
20	211.0
21	327.0
22	466.0
23	675.0
24	891.0
25	1206.0
26	1479.0
27	1764.0
28	2115.0
29	2449.0
30	2856.0
31	3783.0
32	4711.0
33	6743.0
34	10226.0
35	12799.0
36	14127.0
37	22488.0
38	29621.0
39	33198.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.655271925912324	19.78447936616208	23.793845265891857	11.766403442033738
2	31.762074664847706	24.68517984101582	25.557494031534482	17.99525146260199
3	32.59765983681822	24.61828055723168	25.747042002256208	17.03701760369389
4	29.33927119133195	25.371225437468848	26.003489256762073	19.286014114437126
5	25.182989217409556	30.61626046121153	24.197208594590343	20.00354172678857
6	26.51048088779285	36.03838182438282	23.777448382611432	13.673688905212897
7	60.09720072408636	28.623055329642945	6.798147808064643	4.48159613820605
8	80.75268253010468	6.891282105097463	8.033161056746334	4.322874308051525
9	73.91124695017972	9.661043628827032	10.22247291234882	6.205236508644437
10	41.34966025657843	28.655193220872576	16.949786184642022	13.045360337906969
11	32.83246320539392	27.70810924259517	22.580475903140332	16.878951648870583
12	32.048036309258336	23.968308103995593	26.877115197943173	17.106540388802895
13	25.693916100427632	25.32072303696513	27.54151690846604	21.443843954141197
14	19.832358265340925	29.33992706666317	27.36180706771257	23.46590760028334
15	20.10979353044573	28.120654825930686	32.98397040690506	18.785581236718524
16	22.69328646010966	26.384552824199176	31.647952357215942	19.27420835847522
17	22.055775638166697	25.960201484901752	27.54807566177821	24.435947215153345
18	23.473778104257942	28.01177952094866	26.730854999081778	21.783587375711626
19	26.692814229871185	28.52598578062282	24.51661988089304	20.264580108612957
20	28.733242385287404	25.965448487551484	26.325524044389642	18.975785082771466
21	26.074979667864735	27.637930582154947	26.290762651835138	19.996327098145183
22	24.277881260330037	26.299944906472177	26.13794369966157	23.28423013353622
23	24.220164231182935	27.550043287771857	27.403783088910462	20.826009392134743
24	23.963061101345858	25.18823622005929	28.043917412178292	22.80478526641656
25	23.389170186530944	27.05616916336543	25.936589972977934	23.61807067712569
26	22.228926725607998	29.309756801427184	25.920193089697513	22.54112338326731
27	22.60080803840806	29.404858724453657	26.47047249258861	21.523860744549676
28	21.03064249547446	29.656058976309787	27.805834666946506	21.50746386126925
29	23.425899205079098	27.384762704305167	27.57693417635176	21.61240391426398
30	23.715140226145813	26.49867513183094	27.430018102159142	22.356166539864102
31	23.631188183750034	28.27281790277304	25.704410105727106	22.391583807749825
32	25.125272188262453	28.64141983891702	25.605372930713333	20.627935042107197
33	24.68977096833434	28.748983393236614	25.699163103077368	20.862082535351682
34	23.172075451898102	28.522050528635518	27.864207571424828	20.441666448041556
35	23.246189364325627	26.825956922108247	27.109295065194004	22.818558648372118
36	22.520135372668364	30.178791615289768	26.227142744707088	21.073930267334784
37	23.44557546501561	29.56620405593305	27.114542067843743	19.8736784112076
38	22.94645433795944	27.34409843376971	27.138809455098773	22.570637773172074
39	24.14801794474906	26.46522548993887	27.81960804890207	21.56714851641
40	25.23677099456935	25.47485373980114	28.91819922869061	20.370176036938897
41	20.46068683264685	27.74155888448724	29.187763989820816	22.609990293045097
42	22.143007057218565	27.269984521342185	27.550043287771857	23.03696513366739
43	23.137969934674818	27.290972531941126	26.927617598446886	22.643439934937167
44	22.52800587664297	27.95340661647034	26.70921111315161	22.80937639373508
45	22.810032269066298	26.61410919012514	25.76278301020542	24.813075530603143
46	21.843272030852376	28.960831125219716	26.08678542382664	23.109111420101268
47	22.087257654065116	27.31589579452738	28.489912637405883	22.106933914001626
48	22.45454783954666	25.0885431697143	29.087415064144608	23.369493926594433
49	20.64695542671249	26.62985019807435	28.734554135949843	23.98864023926332
50	19.709709578403338	26.413411338772725	29.093973817456774	24.78290526536716
51	19.82645538735997	26.429152346721935	27.88453970669255	25.859852559225544
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	89.0
1	205.5
2	322.0
3	508.0
4	694.0
5	515.5
6	337.0
7	308.5
8	280.0
9	292.0
10	304.0
11	310.5
12	317.0
13	326.0
14	335.0
15	315.5
16	296.0
17	362.5
18	429.0
19	505.0
20	581.0
21	624.0
22	667.0
23	695.5
24	724.0
25	908.0
26	1369.5
27	1647.0
28	1993.5
29	2340.0
30	2637.5
31	2935.0
32	3535.5
33	4136.0
34	4699.0
35	5262.0
36	5199.5
37	5137.0
38	5783.0
39	6429.0
40	7187.0
41	7945.0
42	8984.5
43	10024.0
44	11112.5
45	12201.0
46	12111.5
47	12022.0
48	11599.0
49	11176.0
50	10370.5
51	9565.0
52	8992.0
53	8419.0
54	7928.5
55	7438.0
56	7421.0
57	7404.0
58	7547.0
59	7690.0
60	7590.5
61	7491.0
62	6804.0
63	6117.0
64	5031.5
65	3946.0
66	3142.5
67	2339.0
68	2040.0
69	1741.0
70	1386.0
71	1031.0
72	844.0
73	657.0
74	501.0
75	290.0
76	235.0
77	191.5
78	148.0
79	116.5
80	85.0
81	68.5
82	52.0
83	36.5
84	21.0
85	14.5
86	8.0
87	6.5
88	5.0
89	5.5
90	6.0
91	4.5
92	3.0
93	1.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	152468.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.725660466458535
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.07234964212458	31.126531468898396
2	10.346113573446935	9.461657528137051
3	5.96411205301433	8.181388881601386
4	3.994721516990117	7.306451189757851
5	2.8515283216432143	6.5194007922974
6	2.1587274265960956	5.922554240889891
7	1.5835449029648434	5.068604559645302
8	1.2608115667627695	4.612115329118241
9	1.0126654904829524	4.167431854553087
>10	2.722434987162385	16.056483983524412
>50	0.02008118536368461	0.5988141774011596
>100	0.012909333448082963	0.9785659941758271
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	274	0.1797098407534696	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	258	0.1692158354539969	No Hit
GCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	154	0.10100480100742452	Illumina PCR Primer Index 5 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08198441640213028	0.0
2	0.0	0.0	0.0	0.41516908466038777	0.0
3	0.0	0.0	0.0	0.5496235275598814	0.0
4	0.0	0.0	0.0	0.7208069890075295	0.0
5	0.0	0.0	0.0	1.2638717632552405	0.0
6	0.0	0.0	0.0	1.5760684209145526	0.0
7	0.0	0.0	0.0	1.8889209539050817	0.0
8	0.0	0.0	0.0	2.4418238581210483	0.0
9	0.0	0.0	0.0	2.6320277041739906	0.0
10	0.0	0.0	0.0	3.2492063908492272	0.0
11	0.0	0.0	0.0	3.9372196132959045	0.0
12	0.0	0.0	0.0	4.625888711073799	0.0
13	0.0	0.0	0.0	4.88495946690453	0.0
14	0.0	0.0	0.0	5.026628538447412	0.0
15	0.0	0.0	0.0	5.250282026392424	0.0
16	0.0	0.0	0.0	5.700212503607315	0.0
17	0.0	0.0	0.0	6.152110606815857	0.0
18	0.0	0.0	0.0	6.72993677361807	0.0
19	0.0	0.0	0.0	7.037542303958864	0.0
20	0.0	0.0	0.0	7.388435606159981	0.0
21	0.0	0.0	0.0	7.785896056877509	0.0
22	0.0	0.0	0.0	8.179421255607734	0.0
23	0.0	0.0	0.0	8.594590340268121	0.0
24	0.0	0.0	0.0	8.911378125245953	0.0
25	0.0	0.0	0.0	9.192748642338064	0.0
26	0.0	0.0	0.0	9.468872156780439	0.0
27	0.0	0.0	0.0	9.733189915260906	0.0
28	0.0	0.0	0.0	10.053257076894823	0.0
29	0.0	0.0	0.0	10.362830233229268	0.0
30	0.0	0.0	0.0	10.708476532780649	0.0
31	0.0	0.0	0.0	11.046252328357426	0.0
32	0.0	0.0	0.0	11.342052102736313	0.0
33	0.0	0.0	0.0	11.671957394338484	0.0
34	0.0	0.0	0.0	12.007109688590393	0.0
35	0.0	0.0	0.0	12.33570322953013	0.0
36	0.0	0.0	0.0	12.680037778419079	0.0
37	0.0	0.0	0.0	13.041425085919668	0.0
38	0.0	0.0	0.0	13.38707138547105	0.0
39	0.0	0.0	0.0	13.760920324264763	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGGA	45	3.8016879E-10	45.000004	4
ATGCCGC	25	3.8748498E-5	45.000004	41
ACAACGA	20	7.014027E-4	45.0	13
CATTCTA	20	7.014027E-4	45.0	45
CAGGGTA	40	6.7484507E-9	45.0	5
GAGCGAC	20	7.014027E-4	45.0	18
GCGGGTC	20	7.014027E-4	45.0	5
CGGGAAC	20	7.014027E-4	45.0	6
TTCGGGA	35	1.2026794E-7	45.0	4
GGACCCA	20	7.014027E-4	45.0	8
ATTTGGA	20	7.014027E-4	45.0	16
TGCGGGA	80	0.0	42.1875	4
CATACGA	65	0.0	41.53846	18
ACATACG	65	0.0	41.53846	17
CAAGCGA	45	1.909575E-8	40.000004	15
CGAGATA	45	1.909575E-8	40.000004	19
TCGACAC	40	3.4319783E-7	39.375	34
GTTATGG	40	3.4319783E-7	39.375	2
CGTTTTT	180	0.0	38.750004	1
CCCTAGC	35	6.2127074E-6	38.571426	21
>>END_MODULE