FastQCFastQC Report
Sat 18 Jun 2016
SRR3551149_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551149_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162283
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5050.31118478213984213No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGCG207.0153666E-445.00000420
GCGCGAC207.0153666E-445.0000049
TAGGTAT253.8759597E-545.0000046
CGGTTCG302.1539508E-645.00000429
TTGTCCG302.1539508E-645.00000424
CACGCCA207.0153666E-445.00000427
ACACAAC207.0153666E-445.00000411
CGCGACC207.0153666E-445.00000410
CCTCGTC207.0153666E-445.00000436
GCGTTAG207.0153666E-445.0000041
GGCCCGC406.7520887E-945.00000422
CGTTAGG253.8759597E-545.0000042
CGAGGGT207.0153666E-445.0000045
TCAGACG253.8759597E-545.00000422
TCGTCCC207.0153666E-445.00000438
CGGGATC253.8759597E-545.0000046
AGCGACT207.0153666E-445.00000419
AGTTACC207.0153666E-445.00000410
GGCGATA351.203316E-745.0000048
AGCTACG253.8759597E-545.0000049