##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551149_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	162283
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.03951122421942	33.0	31.0	34.0	30.0	34.0
2	32.152049198005955	33.0	31.0	34.0	30.0	34.0
3	32.041544708934396	33.0	31.0	34.0	30.0	34.0
4	35.787180419390815	37.0	35.0	37.0	35.0	37.0
5	35.87327076773291	37.0	35.0	37.0	35.0	37.0
6	35.85922739904981	37.0	35.0	37.0	35.0	37.0
7	26.769883475163756	36.0	10.0	37.0	0.0	37.0
8	31.142849220189422	36.0	19.0	37.0	17.0	37.0
9	35.955515981341236	39.0	32.0	39.0	32.0	39.0
10	36.70704879747108	38.0	35.0	39.0	33.0	39.0
11	37.06445530338976	39.0	37.0	39.0	34.0	39.0
12	37.07781468175964	39.0	37.0	39.0	34.0	39.0
13	37.04756505610569	39.0	37.0	39.0	33.0	39.0
14	38.22872389591023	40.0	37.0	41.0	33.0	41.0
15	38.322128627151336	40.0	37.0	41.0	34.0	41.0
16	38.252189077106046	40.0	37.0	41.0	34.0	41.0
17	38.12857169266035	40.0	37.0	41.0	33.0	41.0
18	38.043325548578714	40.0	37.0	41.0	33.0	41.0
19	37.941823850927086	40.0	37.0	41.0	33.0	41.0
20	37.99465748106702	40.0	36.0	41.0	34.0	41.0
21	37.92744156812482	40.0	36.0	41.0	33.0	41.0
22	37.92307881909997	40.0	36.0	41.0	33.0	41.0
23	37.871151013969424	40.0	36.0	41.0	33.0	41.0
24	37.83048748174485	40.0	36.0	41.0	33.0	41.0
25	37.726588736959506	40.0	36.0	41.0	33.0	41.0
26	37.617723359809716	40.0	36.0	41.0	33.0	41.0
27	37.47387588348749	40.0	36.0	41.0	33.0	41.0
28	37.39875402845646	40.0	36.0	41.0	33.0	41.0
29	37.37356962836526	40.0	36.0	41.0	33.0	41.0
30	37.27527837173333	39.0	35.0	41.0	32.0	41.0
31	37.17059088136157	39.0	35.0	41.0	32.0	41.0
32	37.038851882205776	39.0	35.0	41.0	31.0	41.0
33	36.908425405002376	39.0	35.0	41.0	31.0	41.0
34	36.7762612226789	39.0	35.0	41.0	31.0	41.0
35	36.68551234571705	39.0	35.0	41.0	31.0	41.0
36	36.549077845492135	39.0	35.0	41.0	30.0	41.0
37	36.469205030717944	39.0	35.0	41.0	30.0	41.0
38	36.37362508703931	39.0	35.0	41.0	30.0	41.0
39	36.34298725066704	39.0	35.0	41.0	30.0	41.0
40	36.149726095770966	39.0	35.0	40.0	30.0	41.0
41	36.02330496724857	39.0	35.0	40.0	29.0	41.0
42	35.97122927232058	38.0	35.0	40.0	29.0	41.0
43	35.92722589550353	38.0	35.0	40.0	29.0	41.0
44	35.7329171878755	38.0	35.0	40.0	28.0	41.0
45	35.64778812321685	38.0	35.0	40.0	28.0	41.0
46	35.530536162136514	38.0	34.0	40.0	27.0	41.0
47	35.398316521138995	38.0	34.0	40.0	26.0	41.0
48	35.317007942914536	38.0	34.0	40.0	26.0	41.0
49	35.151494611265505	38.0	34.0	40.0	26.0	41.0
50	34.94377722866844	38.0	34.0	40.0	26.0	41.0
51	32.946876752340046	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	16.0
10	10.0
11	9.0
12	9.0
13	7.0
14	6.0
15	12.0
16	20.0
17	49.0
18	75.0
19	122.0
20	181.0
21	359.0
22	465.0
23	668.0
24	922.0
25	1298.0
26	1617.0
27	1835.0
28	2108.0
29	2537.0
30	3121.0
31	4047.0
32	5136.0
33	7279.0
34	11838.0
35	14327.0
36	14950.0
37	24295.0
38	31058.0
39	33894.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.803004627718245	20.88820147520073	23.726453171311846	12.582340725769182
2	32.32008281828657	24.331568925888725	25.724197851900694	17.62415040392401
3	33.289377199090474	23.972936167066177	26.76189126402642	15.975795369816925
4	29.80349143163486	26.014431579401414	25.201037693412125	18.9810392955516
5	25.8585310845868	31.129569948793158	23.789922542718582	19.221976423901456
6	27.114978155444504	35.6623922407153	23.22670889741994	13.995920706420264
7	60.893007893617934	28.72882557014598	6.110313464749851	4.267853071486231
8	82.23597049598541	6.467097601104244	7.776538516049124	3.520393386861224
9	76.26183888638982	8.843193680175988	9.59003715731161	5.304930276122576
10	42.19172679824751	28.385597998558076	16.428707874515506	12.993967328678913
11	32.35335802271341	26.854322387434298	23.82874361454989	16.963575975302405
12	32.00951424363612	22.878551665916945	26.960926283098047	18.15100780734889
13	25.90166560884381	24.30260717388759	27.986295545436057	21.809431671832538
14	20.251042931175785	28.529174343584973	28.88102882002428	22.338753905214965
15	20.74955479008892	26.759426434068878	32.42422188399278	20.06679689184942
16	23.9673902996617	25.42472101205918	31.136348231176402	19.471540457102716
17	23.337626245509387	25.812315522882866	27.68989974304148	23.16015848856627
18	24.62303506836822	27.154415434765195	27.223430673576406	20.99911882329018
19	26.246125595410486	28.42503527787877	25.379737865334018	19.949101261376732
20	28.25188097336135	25.708792664666046	26.933196946075682	19.106129415896923
21	26.234417653112157	27.79650363870522	26.705200175002926	19.263878533179692
22	24.940998132891306	25.303944344139556	27.14270749246686	22.61235003050227
23	24.83994010463203	26.127813757448408	28.13849879531436	20.893747342605202
24	24.562030526919024	25.300247099203244	28.646869974057665	21.490852399820067
25	23.171250223375214	26.495073421122363	27.2801217625999	23.053554592902522
26	22.945718282260003	28.586481640097855	26.2368824830697	22.230917594572443
27	21.887073815495153	29.540987041156498	26.870959989647712	21.700979153700633
28	21.296747040663533	28.728209362656592	29.549613946007902	20.425429650671976
29	22.693073211611814	26.85247376496614	28.227232673785917	22.227220349636127
30	23.34871798031833	26.780377488707995	27.804514336067243	22.06639019490643
31	25.252182915031153	26.798247505900186	26.47843581890894	21.471133760159724
32	25.912757343652753	27.38672565826365	25.907211476248282	20.79330552183531
33	25.026650973915938	28.206897826636183	25.92631390841924	20.840137291028636
34	23.125650869160662	26.91162968394718	28.57230886784198	21.39041057905018
35	23.12010500175619	26.88081930947789	27.697294232914103	22.301781455851817
36	24.1935384482663	29.342568229574262	25.448136896655843	21.015756425503596
37	23.197747145418806	29.953846059045002	26.116105815150075	20.732300980386114
38	23.284632401422208	28.8323484283628	25.1412655669417	22.741753603273292
39	24.673564082497858	26.557926585039716	26.380458828096597	22.38805050436583
40	24.284737156695403	25.80307241054208	28.025116617267365	21.887073815495153
41	20.97816776865106	27.113129532976345	28.164379509868564	23.744323188504033
42	22.29623558844734	27.893864422028187	26.536359322911213	23.27354066661326
43	23.070808402605326	26.244276972942327	26.382307450564753	24.30260717388759
44	22.37449393959934	26.87404102709464	27.19446892157527	23.55699611173074
45	23.067727365158397	26.575796602231904	26.32007049413679	24.036405538472916
46	21.550008318801105	28.837278088277884	26.241195935495398	23.37151765742561
47	21.876598288175593	27.39165531817874	28.187795394465226	22.543950999180442
48	23.101618777074616	25.191794581071335	28.61667580707776	23.089910834776287
49	21.93082454724155	26.085911648170175	28.5193150237548	23.463948780833483
50	20.0113382178047	27.08416778097521	28.383133168600533	24.52136083261956
51	20.71874441561963	26.7193729472588	27.38117979085918	25.180702846262392
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	87.0
1	253.0
2	419.0
3	738.0
4	1057.0
5	745.5
6	434.0
7	414.5
8	395.0
9	365.5
10	336.0
11	338.5
12	341.0
13	339.0
14	337.0
15	344.0
16	351.0
17	362.5
18	374.0
19	470.5
20	567.0
21	690.0
22	813.0
23	847.0
24	881.0
25	1117.0
26	1643.5
27	1934.0
28	2343.0
29	2752.0
30	3098.0
31	3444.0
32	3889.0
33	4334.0
34	4988.0
35	5642.0
36	5788.0
37	5934.0
38	6230.0
39	6526.0
40	7333.5
41	8141.0
42	8580.0
43	9019.0
44	9308.0
45	9597.0
46	10173.5
47	10750.0
48	10838.0
49	10926.0
50	11063.5
51	11201.0
52	10715.0
53	10229.0
54	9564.5
55	8900.0
56	8865.0
57	8830.0
58	8573.5
59	8317.0
60	8245.5
61	8174.0
62	7383.0
63	6592.0
64	5471.0
65	4350.0
66	3718.0
67	3086.0
68	2542.0
69	1998.0
70	1707.0
71	1416.0
72	1169.0
73	922.0
74	762.5
75	529.5
76	456.0
77	338.0
78	220.0
79	163.0
80	106.0
81	83.5
82	61.0
83	45.5
84	30.0
85	25.5
86	21.0
87	17.0
88	13.0
89	11.5
90	10.0
91	6.5
92	3.0
93	1.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	162283.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.85610322707863
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.34625057959	29.535441173752027
2	10.042011493445365	8.808069853280996
3	5.9420269491787385	7.817824417837975
4	4.059237610824633	7.120893747342604
5	2.8930322743814196	6.343856103227079
6	2.2804232060811285	6.000628531639174
7	1.7169914712453105	5.271038864206356
8	1.3334082702224221	4.6782472594171916
9	1.1015722696041927	4.3479600451063884
>10	3.255539475348105	18.727161809924638
>50	0.022481066726616178	0.7018603304104558
>100	0.0056202666816540445	0.3358330817152752
>500	0.0014050666704135111	0.31118478213984213
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	505	0.31118478213984213	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05915591898103929	0.0
2	0.0	0.0	0.0	0.28592027507502327	0.0
3	0.0	0.0	0.0	0.39868624563262944	0.0
4	0.0	0.0	0.0	0.5293222333824246	0.0
5	0.0	0.0	0.0	0.9280084790150539	0.0
6	0.0	0.0	0.0	1.1972911518766598	0.0
7	0.0	0.0	0.0	1.430833790353888	0.0
8	0.0	0.0	0.0	1.8923731999038715	0.0
9	0.0	0.0	0.0	2.0655275044212886	0.0
10	0.0	0.0	0.0	2.52891553643943	0.0
11	0.0	0.0	0.0	3.113080236377193	0.0
12	0.0	0.0	0.0	3.5672251560545467	0.0
13	0.0	0.0	0.0	3.8081622844044047	0.0
14	0.0	0.0	0.0	3.9196958399832393	0.0
15	0.0	0.0	0.0	4.121195689012405	0.0
16	0.0	0.0	0.0	4.481677070303112	0.0
17	0.0	0.0	0.0	4.882211938403899	0.0
18	0.0	0.0	0.0	5.33389202812371	0.0
19	0.0	0.0	0.0	5.604407115964087	0.0
20	0.0	0.0	0.0	5.880468071208938	0.0
21	0.0	0.0	0.0	6.195966305774481	0.0
22	0.0	0.0	0.0	6.537345254894228	0.0
23	0.0	0.0	0.0	6.86331901677933	0.0
24	0.0	0.0	0.0	7.150471706833125	0.0
25	0.0	0.0	0.0	7.396338495098069	0.0
26	0.0	0.0	0.0	7.645902528299329	0.0
27	0.0	0.0	0.0	7.874515506861471	0.0
28	0.0	0.0	0.0	8.132090237424746	0.0
29	0.0	0.0	0.0	8.390897382966793	0.0
30	0.0	0.0	0.0	8.682363525446288	0.0
31	0.0	0.0	0.0	8.976910705372713	0.0
32	0.0	0.0	0.0	9.244960963255547	0.0
33	0.0	0.0	0.0	9.534578483266886	0.0
34	0.0	0.0	0.0	9.81310426846928	0.0
35	0.0	0.0	0.0	10.116278353247106	0.0
36	0.0	0.0	0.0	10.400350005853971	0.0
37	0.0	0.0	0.0	10.673329923651892	0.0
38	0.0	0.0	0.0	10.994374025621907	0.0
39	0.0	0.0	0.0	11.304942600272364	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGCG	20	7.0153666E-4	45.000004	20
GCGCGAC	20	7.0153666E-4	45.000004	9
TAGGTAT	25	3.8759597E-5	45.000004	6
CGGTTCG	30	2.1539508E-6	45.000004	29
TTGTCCG	30	2.1539508E-6	45.000004	24
CACGCCA	20	7.0153666E-4	45.000004	27
ACACAAC	20	7.0153666E-4	45.000004	11
CGCGACC	20	7.0153666E-4	45.000004	10
CCTCGTC	20	7.0153666E-4	45.000004	36
GCGTTAG	20	7.0153666E-4	45.000004	1
GGCCCGC	40	6.7520887E-9	45.000004	22
CGTTAGG	25	3.8759597E-5	45.000004	2
CGAGGGT	20	7.0153666E-4	45.000004	5
TCAGACG	25	3.8759597E-5	45.000004	22
TCGTCCC	20	7.0153666E-4	45.000004	38
CGGGATC	25	3.8759597E-5	45.000004	6
AGCGACT	20	7.0153666E-4	45.000004	19
AGTTACC	20	7.0153666E-4	45.000004	10
GGCGATA	35	1.203316E-7	45.000004	8
AGCTACG	25	3.8759597E-5	45.000004	9
>>END_MODULE