##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3551148_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 105117 Sequences flagged as poor quality 0 Sequence length 51 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.99154275711826 33.0 31.0 34.0 30.0 34.0 2 32.11985692133528 33.0 31.0 34.0 30.0 34.0 3 32.01572533462713 33.0 31.0 34.0 30.0 34.0 4 35.76008637993854 37.0 35.0 37.0 35.0 37.0 5 35.83592568281058 37.0 35.0 37.0 35.0 37.0 6 35.821950778656166 37.0 35.0 37.0 35.0 37.0 7 26.794048536392783 35.0 10.0 37.0 0.0 37.0 8 31.169962993616636 36.0 19.0 37.0 17.0 37.0 9 35.89598257180094 39.0 32.0 39.0 32.0 39.0 10 36.690430662975544 38.0 35.0 39.0 33.0 39.0 11 37.073128038281155 39.0 37.0 39.0 34.0 39.0 12 37.1136067429626 39.0 37.0 39.0 34.0 39.0 13 37.04398907883596 39.0 37.0 39.0 33.0 39.0 14 38.20367780663451 40.0 37.0 41.0 33.0 41.0 15 38.30275787931543 40.0 37.0 41.0 34.0 41.0 16 38.24086494097054 40.0 37.0 41.0 34.0 41.0 17 38.15868032763492 40.0 37.0 41.0 34.0 41.0 18 38.0837542928356 40.0 37.0 41.0 33.0 41.0 19 37.99113368912735 40.0 37.0 41.0 33.0 41.0 20 38.018874206836195 40.0 37.0 41.0 34.0 41.0 21 37.94229287365507 40.0 36.0 41.0 33.0 41.0 22 37.92620603708249 40.0 36.0 41.0 33.0 41.0 23 37.87854485953747 40.0 36.0 41.0 33.0 41.0 24 37.83667722632876 40.0 36.0 41.0 33.0 41.0 25 37.7282076162752 40.0 36.0 41.0 33.0 41.0 26 37.59419503981278 40.0 36.0 41.0 33.0 41.0 27 37.46674657762303 39.0 36.0 41.0 33.0 41.0 28 37.43586670091422 39.0 36.0 41.0 33.0 41.0 29 37.42575415965067 40.0 36.0 41.0 33.0 41.0 30 37.378758906742014 40.0 36.0 41.0 32.0 41.0 31 37.26348735218851 40.0 35.0 41.0 32.0 41.0 32 37.109487523426274 39.0 35.0 41.0 32.0 41.0 33 37.033134507263334 39.0 35.0 41.0 31.0 41.0 34 36.88385323021015 39.0 35.0 41.0 31.0 41.0 35 36.83055071967427 39.0 35.0 41.0 31.0 41.0 36 36.684599065802864 39.0 35.0 41.0 31.0 41.0 37 36.581789815158345 39.0 35.0 41.0 30.0 41.0 38 36.47953233064109 39.0 35.0 41.0 30.0 41.0 39 36.45099270336863 39.0 35.0 41.0 30.0 41.0 40 36.260604849833996 39.0 35.0 40.0 30.0 41.0 41 36.18136933131653 39.0 35.0 40.0 30.0 41.0 42 36.12504162028977 39.0 35.0 40.0 29.0 41.0 43 36.10107784658999 39.0 35.0 40.0 29.0 41.0 44 35.86018436599218 38.0 35.0 40.0 28.0 41.0 45 35.78116765128381 38.0 35.0 40.0 28.0 41.0 46 35.71042742848445 38.0 35.0 40.0 28.0 41.0 47 35.56260167242216 38.0 34.0 40.0 27.0 41.0 48 35.45154446949589 38.0 34.0 40.0 27.0 41.0 49 35.27772862619747 38.0 34.0 40.0 26.0 41.0 50 35.07375591008115 38.0 34.0 40.0 26.0 41.0 51 32.94021899407327 36.0 31.0 39.0 20.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 6.0 9 18.0 10 12.0 11 7.0 12 7.0 13 1.0 14 8.0 15 5.0 16 10.0 17 16.0 18 36.0 19 83.0 20 128.0 21 221.0 22 313.0 23 423.0 24 588.0 25 748.0 26 991.0 27 1176.0 28 1361.0 29 1688.0 30 2020.0 31 2512.0 32 3247.0 33 4560.0 34 7275.0 35 9399.0 36 9834.0 37 15504.0 38 20298.0 39 22618.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.487266569631934 21.128837390717013 25.354604868860413 13.029291170790644 2 30.808527640629013 26.09758649885366 25.212858053407157 17.881027807110172 3 32.70546153333904 24.651578717048622 25.401219593405443 17.241740156206895 4 28.909691106100823 25.754159650674964 25.827411360674297 19.50873788254992 5 26.589419408849185 29.94853353881865 23.9609197370549 19.501127315277262 6 25.031155759772446 36.371852316942075 24.571667760685713 14.02532416259977 7 60.33752865854238 28.518698212468014 6.797187895392753 4.34658523359685 8 81.19619091108004 6.614534280848959 7.907379396291751 4.281895411779256 9 73.89194897114643 9.500841919004538 10.424574521723413 6.182634588125612 10 40.559566958722186 29.54802743609502 16.905923875300854 12.986481729881941 11 31.480160202441088 26.656011872484946 24.426115661596125 17.437712263477838 12 29.861012015183082 23.175128666152954 28.07633398974476 18.887525328919207 13 24.068418999781198 26.983266265209245 29.59464216064005 19.35367257436951 14 19.992008904363708 30.629679309721546 27.71292940247534 21.665382383439404 15 18.7857339916474 27.949808308836822 33.926957580600664 19.337500118915113 16 21.78429749707469 26.9157224806644 32.17177050334389 19.128209518917018 17 22.19431680888914 26.085219327035592 28.013546809745332 23.70691705432994 18 22.74322897342961 27.16877384248028 29.500461390640904 20.587535793449206 19 25.800774375219994 26.5618311024858 27.315277262478954 20.322117259815254 20 25.796017770674585 27.654898827021317 27.26295461247943 19.286128789824673 21 24.344302063415054 27.55501013156768 28.721329566102533 19.379358238914733 22 22.127724345253384 27.001341362481806 27.451316152477716 23.419618139787094 23 22.165777181616676 27.31052065793354 29.52424441336796 20.999457747081824 24 22.015468477981678 26.24599256067049 29.94853353881865 21.790005422529184 25 21.93746016343693 28.088701161562827 27.906047547019035 22.067791127981202 26 20.265038005270316 29.80678672336539 27.60067353520363 22.32750173616066 27 20.465766717086677 29.888600321546466 28.277062701561118 21.368570259805743 28 18.968387606191197 28.781262783374718 30.44417173245051 21.80617787798358 29 20.206056108907216 28.37885403883292 29.862914657001244 21.552175195258616 30 21.080320024353817 27.49032030975009 28.92300959882797 22.506350067068123 31 23.452914371604976 27.527421825204296 27.455121436114045 21.564542367076687 32 23.634616665239687 27.763349410656694 27.985007182472867 20.617026741630752 33 22.583407060703784 27.58735504247648 27.8061588515654 22.023079045254335 34 21.94887601434592 27.07459307248114 29.739242938820553 21.23728797435239 35 22.00024734343636 26.335416726124222 28.108678900653555 23.555657029785856 36 22.494934216159137 27.965029443382132 28.03542719065422 21.504609149804505 37 22.367457214342114 27.96407812247305 28.884956762464682 20.78350790072015 38 20.973772082536602 28.015449451563494 28.92491224064614 22.085866225253763 39 23.373003415242064 25.698983037948192 28.82407222428342 22.103941322526328 40 23.76685027160212 25.1139206788626 29.353957970642234 21.765271078893043 41 21.122178144353434 26.361102390669444 27.884167166110146 24.632552298866976 42 21.531246135258808 26.700723955211814 28.72798881246611 23.04004109706327 43 23.017209395245295 25.589581133403733 28.393123852469156 23.00008561888182 44 21.35239780435134 26.763511135211242 29.335882873369673 22.548208187067743 45 21.34668987889685 26.996584757936393 27.385675009751036 24.271050353415717 46 21.88323487161924 27.4684399288412 27.701513551566347 22.946811647973213 47 21.536002739804218 25.561041506131264 29.95709542700039 22.94586032706413 48 21.57405557616751 24.79427685341096 30.054130159726782 23.57753741069475 49 21.313393647078968 25.62763396976702 30.03224977881789 23.02672260433612 50 20.200348183452725 25.903517033400874 29.93616636700058 23.95996841614582 51 21.068904173444828 25.862610234310342 27.636823729748755 25.431661862496075 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 61.0 1 154.0 2 247.0 3 416.0 4 585.0 5 443.0 6 301.0 7 259.5 8 218.0 9 203.5 10 189.0 11 201.5 12 214.0 13 215.5 14 217.0 15 258.5 16 300.0 17 286.0 18 272.0 19 299.0 20 326.0 21 437.5 22 549.0 23 614.5 24 680.0 25 814.0 26 1076.5 27 1205.0 28 1569.5 29 1934.0 30 2291.0 31 2648.0 32 2820.5 33 2993.0 34 3064.0 35 3135.0 36 3412.0 37 3689.0 38 4137.0 39 4585.0 40 5149.5 41 5714.0 42 6382.5 43 7051.0 44 7422.5 45 7794.0 46 8379.0 47 8964.0 48 9089.5 49 9215.0 50 8612.5 51 8010.0 52 7126.0 53 6242.0 54 5591.0 55 4940.0 56 4808.0 57 4676.0 58 4505.0 59 4334.0 60 4197.0 61 4060.0 62 3577.5 63 3095.0 64 2568.5 65 2042.0 66 1627.5 67 1213.0 68 1034.0 69 855.0 70 720.5 71 586.0 72 491.5 73 397.0 74 339.5 75 223.0 76 164.0 77 138.5 78 113.0 79 73.5 80 34.0 81 26.0 82 18.0 83 12.5 84 7.0 85 7.0 86 7.0 87 4.5 88 2.0 89 3.0 90 4.0 91 2.0 92 0.0 93 1.0 94 2.0 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 105117.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.24259634502507 #Duplication Level Percentage of deduplicated Percentage of total 1 69.90535642368103 33.02510535879068 2 10.22351993556182 9.659712510821276 3 5.49738219895288 7.7913182453837155 4 3.5420861860652435 6.693493916302787 5 2.6560612162706403 6.273961395397509 6 1.9975835682641965 5.662262050857616 7 1.4377768828030608 4.754701903593139 8 1.1377366089407974 4.2999705090518185 9 1.000805477245268 4.255258426324953 >10 2.559403946838502 15.900377674400906 >50 0.03624647603705195 1.2766726599883937 >100 0.006041079339508659 0.40716534908720764 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 220 0.20929059999809735 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.08371623999923894 0.0 2 0.0 0.0 0.0 0.46043931999581417 0.0 3 0.0 0.0 0.0 0.6402389718123614 0.0 4 0.0 0.0 0.0 0.890436370900996 0.0 5 0.0 0.0 0.0 1.6476878145304756 0.0 6 0.0 0.0 0.0 2.0586584472540124 0.0 7 0.0 0.0 0.0 2.4829475727047003 0.0 8 0.0 0.0 0.0 3.2306858072433573 0.0 9 0.0 0.0 0.0 3.511325475422624 0.0 10 0.0 0.0 0.0 4.315191643597134 0.0 11 0.0 0.0 0.0 5.363547285405786 0.0 12 0.0 0.0 0.0 6.300598380851813 0.0 13 0.0 0.0 0.0 6.750573170847722 0.0 14 0.0 0.0 0.0 6.951301882664079 0.0 15 0.0 0.0 0.0 7.222428341752523 0.0 16 0.0 0.0 0.0 7.81414994720169 0.0 17 0.0 0.0 0.0 8.398260985378197 0.0 18 0.0 0.0 0.0 9.142193936280526 0.0 19 0.0 0.0 0.0 9.558872494458555 0.0 20 0.0 0.0 0.0 9.962232559909435 0.0 21 0.0 0.0 0.0 10.433136409905153 0.0 22 0.0 0.0 0.0 10.959216872627643 0.0 23 0.0 0.0 0.0 11.443439215350514 0.0 24 0.0 0.0 0.0 11.81635701171076 0.0 25 0.0 0.0 0.0 12.166443106253032 0.0 26 0.0 0.0 0.0 12.512723917158976 0.0 27 0.0 0.0 0.0 12.825708496247039 0.0 28 0.0 0.0 0.0 13.171037986243899 0.0 29 0.0 0.0 0.0 13.51636747624076 0.0 30 0.0 0.0 0.0 13.896895839873665 0.0 31 0.0 0.0 0.0 14.271716278052075 0.0 32 0.0 0.0 0.0 14.635120865321499 0.0 33 0.0 0.0 0.0 14.998525452590922 0.0 34 0.0 0.0 0.0 15.35527079349677 0.0 35 0.0 0.0 0.0 15.734847836220592 0.0 36 0.0 0.0 0.0 16.132499976216977 0.0 37 0.0 0.0 0.0 16.501612488940893 0.0 38 0.0 0.0 0.0 16.876432927119303 0.0 39 0.0 0.0 0.0 17.276939029842936 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTACCT 20 7.004038E-4 45.000004 8 ACTGCCC 20 7.004038E-4 45.000004 23 ACGTTCT 20 7.004038E-4 45.000004 12 GAGTCGC 20 7.004038E-4 45.000004 25 GACGTTC 20 7.004038E-4 45.000004 11 CGGCAGG 20 7.004038E-4 45.000004 2 ATAGCGG 20 7.004038E-4 45.000004 2 AGAGTCG 20 7.004038E-4 45.000004 24 GAGACGT 20 7.004038E-4 45.000004 9 GTTAATC 20 7.004038E-4 45.000004 40 GAGCGAC 20 7.004038E-4 45.000004 18 AACGAGC 20 7.004038E-4 45.000004 15 AAGGGCG 20 7.004038E-4 45.000004 5 CGCCCAG 20 7.004038E-4 45.000004 19 TTGGGAC 20 7.004038E-4 45.000004 5 TTCTCGC 20 7.004038E-4 45.000004 15 CTCGTAA 25 3.8665836E-5 45.0 33 GAATAGC 25 3.8665836E-5 45.0 27 TAAAACG 25 3.8665836E-5 45.0 21 CGAATAG 25 3.8665836E-5 45.0 26 >>END_MODULE