FastQCFastQC Report
Sat 18 Jun 2016
SRR3551147_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551147_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131295
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2950.2246848699493507No Hit
GCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC2120.16146844891275372No Hit
GAATCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTC1880.14318900186602687No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCG1500.11424654404204272No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCGG253.8718856E-545.027
ATAGGAG207.0104457E-445.05
CTATAGG207.0104457E-445.03
AGGCGAT502.1827873E-1145.07
CGCCGGT253.8718856E-545.028
CTATCTC451.9059371E-840.0000045
GGTACCT403.4271034E-739.3758
CGAAAAA750.039.022
ATCCTGC356.206097E-638.57142620
CTACAGG356.206097E-638.5714262
CACACCC700.038.57142629
AATAGGA356.206097E-638.5714264
AGCATAT301.1344211E-437.50000431
CGGTAGT301.1344211E-437.50000412
TGTACGG301.1344211E-437.5000042
GCATATG301.1344211E-437.50000432
TGCGGGA850.037.0588234
CCATTAT850.037.05882334
TACGGGG552.7102942E-936.818184
GATTGGG552.7102942E-936.818183