##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551147_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	131295
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.98259644312426	33.0	31.0	34.0	30.0	34.0
2	32.10763547736014	33.0	31.0	34.0	30.0	34.0
3	31.98910849613466	33.0	31.0	34.0	30.0	34.0
4	35.75074450664534	37.0	35.0	37.0	35.0	37.0
5	35.81904870710994	37.0	35.0	37.0	35.0	37.0
6	35.787120606268324	37.0	35.0	37.0	35.0	37.0
7	26.804821204158575	35.0	10.0	37.0	0.0	37.0
8	31.134293004303288	35.0	19.0	37.0	17.0	37.0
9	35.85002475341788	39.0	32.0	39.0	32.0	39.0
10	36.64314711146655	38.0	35.0	39.0	33.0	39.0
11	37.01572032446018	39.0	37.0	39.0	34.0	39.0
12	37.03308579915458	39.0	37.0	39.0	34.0	39.0
13	36.977927567691076	39.0	37.0	39.0	33.0	39.0
14	38.15431661525572	40.0	37.0	41.0	33.0	41.0
15	38.256453025629305	40.0	37.0	41.0	33.0	41.0
16	38.192033207662135	40.0	37.0	41.0	34.0	41.0
17	38.071571651624204	40.0	37.0	41.0	33.0	41.0
18	37.984873757568835	40.0	37.0	41.0	33.0	41.0
19	37.887215811721696	40.0	37.0	41.0	33.0	41.0
20	37.943013823831826	40.0	36.0	41.0	33.0	41.0
21	37.90444419056323	40.0	36.0	41.0	33.0	41.0
22	37.88616474351651	40.0	36.0	41.0	33.0	41.0
23	37.87186107620244	40.0	36.0	41.0	33.0	41.0
24	37.809642408317146	40.0	36.0	41.0	33.0	41.0
25	37.677497239041855	40.0	36.0	41.0	33.0	41.0
26	37.56003655889409	40.0	36.0	41.0	33.0	41.0
27	37.436437031113144	39.0	36.0	41.0	33.0	41.0
28	37.33620472980692	39.0	36.0	41.0	32.0	41.0
29	37.2564301763205	39.0	36.0	41.0	32.0	41.0
30	37.20992421645912	39.0	36.0	41.0	32.0	41.0
31	37.02773144445714	39.0	35.0	41.0	31.0	41.0
32	36.92123081610114	39.0	35.0	41.0	31.0	41.0
33	36.82150119958871	39.0	35.0	41.0	31.0	41.0
34	36.66646863932366	39.0	35.0	41.0	30.0	41.0
35	36.61003084656689	39.0	35.0	41.0	30.0	41.0
36	36.47874633459004	39.0	35.0	41.0	30.0	41.0
37	36.40447084809018	39.0	35.0	41.0	30.0	41.0
38	36.24551582314635	39.0	35.0	41.0	30.0	41.0
39	36.21437221524049	39.0	35.0	40.0	30.0	41.0
40	36.02293308960737	39.0	35.0	40.0	29.0	41.0
41	35.96397425644541	39.0	35.0	40.0	29.0	41.0
42	35.92073574774363	38.0	35.0	40.0	29.0	41.0
43	35.85735176510911	38.0	35.0	40.0	29.0	41.0
44	35.69355268669789	38.0	35.0	40.0	28.0	41.0
45	35.57200198027343	38.0	35.0	40.0	27.0	41.0
46	35.40917780570471	38.0	34.0	40.0	26.0	41.0
47	35.28047526562322	38.0	34.0	40.0	26.0	41.0
48	35.181682470771925	38.0	34.0	40.0	26.0	41.0
49	34.97560455462889	38.0	34.0	40.0	25.0	41.0
50	34.77471343158536	38.0	34.0	40.0	24.0	41.0
51	32.56830039224647	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	13.0
10	12.0
11	3.0
12	3.0
13	4.0
14	6.0
15	9.0
16	17.0
17	44.0
18	55.0
19	125.0
20	204.0
21	295.0
22	434.0
23	570.0
24	836.0
25	1065.0
26	1258.0
27	1492.0
28	1819.0
29	2160.0
30	2626.0
31	3351.0
32	4286.0
33	6133.0
34	9287.0
35	11830.0
36	12352.0
37	19500.0
38	25102.0
39	26394.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.65813625804486	20.732701169122965	23.891237290071977	12.717925282760195
2	32.072051487109185	24.559960394531398	25.773258692257894	17.594729426101527
3	32.453634944209604	24.796069918884953	25.954529875471266	16.795765261434177
4	29.583000114246545	25.123576678472144	25.78392170303515	19.509501504246163
5	25.160135572565594	30.17175063787654	24.480749457328915	20.18736433222895
6	26.039072318062377	35.127004074793405	24.687916523858487	14.146007083285731
7	59.77074526828897	28.45272097185727	6.987318633611333	4.789215126242431
8	80.21402185917209	6.169313378270307	8.95464412201531	4.66202064054229
9	72.81236909250161	9.058989298907042	11.545755740888838	6.582885867702502
10	41.37629003389314	26.119044898891808	18.411973037815606	14.092692029399444
11	31.124566815187173	26.46102288739099	24.64221790624167	17.772192391180166
12	29.197608439011386	23.423588103126548	28.928748238699114	18.45005521916295
13	24.92250276095815	25.11367531132183	29.58452340150044	20.379298526219582
14	20.0	28.85334551963136	29.779504169998855	21.36715031036978
15	19.683917894817014	26.652957081381622	34.228264594996	19.43486042880536
16	22.5857801134849	25.697094329563196	31.657717354050043	20.059408202901864
17	21.540805057313683	25.205072546555467	28.903613999009863	24.350508397120986
18	22.966601926958376	26.74587760386915	28.659126394759895	21.628394074412583
19	24.971247953082752	26.593548878479762	26.879926882211812	21.555276286225673
20	25.946913439201797	26.090864084694772	27.286644579001486	20.675577897101945
21	24.746563083133402	26.249285959099737	28.63551544232454	20.368635515442325
22	22.55836094291481	25.02151643246125	28.493849727712405	23.926272896911534
23	22.54084313949503	25.649110781065538	28.99272630336266	22.817319776076776
24	23.034388209756653	24.301001561369436	29.890704139533113	22.773906089340798
25	22.156213107886817	26.71084199702959	27.73220610076545	23.400738794318137
26	20.55219162953654	28.878479759320612	27.65071023268213	22.918618378460717
27	21.712174873376746	27.99801972656994	28.17015118625995	22.119654213793368
28	20.662629955443848	28.04752656232149	29.384972771240335	21.904870710994327
29	21.444837960318367	28.36741688563921	28.27678129403252	21.9109638600099
30	22.801325259910886	26.312502380136333	28.499181233101034	22.386991126851747
31	23.521078487375757	27.390228112266268	26.675806390190033	22.412887010167942
32	24.15781255950341	28.153395026467116	26.3330667580639	21.355725655965575
33	24.29262348147302	26.140370920446323	27.07033778894855	22.496667809132106
34	23.073993678357894	26.351346205110627	28.811455120149283	21.763204996382193
35	21.32906812902243	26.208157203244603	27.915000571232717	24.547774096500248
36	23.349708671312694	27.378803457862066	27.90205262957462	21.36943524125062
37	22.617007502189725	27.7329677443924	28.52050725465555	21.12951749876233
38	22.68784035949579	27.480102060246008	26.42598728055143	23.406070299706766
39	23.97349480178225	25.70699569671351	27.72154308998819	22.59796641151605
40	23.24993335618264	25.217258844586617	29.161049544917933	22.371758254312805
41	20.336646483110552	25.76411896873453	28.884572908336185	25.01466163981873
42	21.677900910164134	25.88674359267299	28.695685288853344	23.739670208309533
43	23.132640237632813	25.927872348528126	28.084085456414943	22.85540195742412
44	22.279599375452225	26.39247496096576	26.99417342625385	24.333752237328156
45	21.664952968506036	26.497581781484442	27.14802543889714	24.68943981111238
46	22.89348413877147	27.163258311436078	27.353669218172815	22.589588331619634
47	21.653528314101834	25.54857382230854	29.571575459842343	23.226322403747286
48	21.29631745306371	24.913363037434785	29.533493278494994	24.256826231006514
49	21.387714688297347	25.06721505007807	30.209832819223887	23.3352374424007
50	20.38463003160821	24.75189458852203	30.249438287825125	24.614037092044633
51	20.07768764994859	25.163943790700333	28.36437031113142	26.393998248219656
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	79.0
1	206.0
2	333.0
3	511.5
4	690.0
5	508.0
6	326.0
7	316.5
8	307.0
9	296.5
10	286.0
11	285.5
12	285.0
13	305.5
14	326.0
15	353.0
16	380.0
17	362.5
18	345.0
19	405.5
20	466.0
21	587.0
22	708.0
23	719.0
24	730.0
25	864.0
26	1314.0
27	1630.0
28	1823.5
29	2017.0
30	2396.0
31	2775.0
32	3054.5
33	3334.0
34	3644.5
35	3955.0
36	4161.5
37	4368.0
38	4832.0
39	5296.0
40	5936.5
41	6577.0
42	7297.0
43	8017.0
44	8622.0
45	9227.0
46	10114.5
47	11002.0
48	11120.5
49	11239.0
50	10676.5
51	10114.0
52	8839.0
53	7564.0
54	6961.0
55	6358.0
56	6164.0
57	5970.0
58	5913.5
59	5857.0
60	5582.0
61	5307.0
62	4841.5
63	4376.0
64	3771.0
65	3166.0
66	2693.5
67	2221.0
68	1920.0
69	1619.0
70	1371.5
71	1124.0
72	925.0
73	726.0
74	598.0
75	401.5
76	333.0
77	255.5
78	178.0
79	136.5
80	95.0
81	74.0
82	53.0
83	41.5
84	30.0
85	18.5
86	7.0
87	11.0
88	15.0
89	13.5
90	12.0
91	6.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	131295.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.113065996420275
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.461982227874	34.60603983396169
2	10.298838453545896	10.11615065310941
3	5.5099793744087595	8.118359419627556
4	3.6443713847060466	7.159450093301345
5	2.648760138331033	6.5044365741269665
6	1.8966239163810616	5.588940934536731
7	1.4236310345362344	4.894321946761111
8	1.093311415411814	4.295670055980807
9	0.8591411689902765	3.7975551239575003
>10	2.1400989408061037	13.545831905251532
>50	0.010855574337422265	0.3853916752351575
>100	0.012406370671339732	0.9878517841501961
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	295	0.2246848699493507	No Hit
GCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC	212	0.16146844891275372	No Hit
GAATCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTC	188	0.14318900186602687	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCG	150	0.11424654404204272	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11729311854983053	0.0
2	0.0	0.0	0.0	0.5933203853916752	0.0
3	0.0	0.0	0.0	0.8187668989679728	0.0
4	0.0	0.0	0.0	1.083818881145512	0.0
5	0.0	0.0	0.0	1.9444761795955672	0.0
6	0.0	0.0	0.0	2.4456376861266613	0.0
7	0.0	0.0	0.0	2.875204691724742	0.0
8	0.0	0.0	0.0	3.650557903956739	0.0
9	0.0	0.0	0.0	3.9475989184660496	0.0
10	0.0	0.0	0.0	4.732853497848357	0.0
11	0.0	0.0	0.0	5.7237518565063406	0.0
12	0.0	0.0	0.0	6.6255379108115315	0.0
13	0.0	0.0	0.0	7.132792566358201	0.0
14	0.0	0.0	0.0	7.371187021592597	0.0
15	0.0	0.0	0.0	7.657565025324651	0.0
16	0.0	0.0	0.0	8.294299097452303	0.0
17	0.0	0.0	0.0	8.944742754864999	0.0
18	0.0	0.0	0.0	9.70105487642332	0.0
19	0.0	0.0	0.0	10.127575307513615	0.0
20	0.0	0.0	0.0	10.573136829277582	0.0
21	0.0	0.0	0.0	11.111618873529077	0.0
22	0.0	0.0	0.0	11.670665295708138	0.0
23	0.0	0.0	0.0	12.171065158612285	0.0
24	0.0	0.0	0.0	12.550363684831867	0.0
25	0.0	0.0	0.0	12.903004684108305	0.0
26	0.0	0.0	0.0	13.243459385353592	0.0
27	0.0	0.0	0.0	13.547355192505426	0.0
28	0.0	0.0	0.0	13.906089340797442	0.0
29	0.0	0.0	0.0	14.318900186602688	0.0
30	0.0	0.0	0.0	14.726379527019308	0.0
31	0.0	0.0	0.0	15.117864351270041	0.0
32	0.0	0.0	0.0	15.509349175520773	0.0
33	0.0	0.0	0.0	15.916066872310447	0.0
34	0.0	0.0	0.0	16.30983662744202	0.0
35	0.0	0.0	0.0	16.71960089873948	0.0
36	0.0	0.0	0.0	17.110324079363266	0.0
37	0.0	0.0	0.0	17.490384249209797	0.0
38	0.0	0.0	0.0	17.917666323927033	0.0
39	0.0	0.0	0.0	18.350279904032902	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCCGG	25	3.8718856E-5	45.0	27
ATAGGAG	20	7.0104457E-4	45.0	5
CTATAGG	20	7.0104457E-4	45.0	3
AGGCGAT	50	2.1827873E-11	45.0	7
CGCCGGT	25	3.8718856E-5	45.0	28
CTATCTC	45	1.9059371E-8	40.000004	5
GGTACCT	40	3.4271034E-7	39.375	8
CGAAAAA	75	0.0	39.0	22
ATCCTGC	35	6.206097E-6	38.571426	20
CTACAGG	35	6.206097E-6	38.571426	2
CACACCC	70	0.0	38.571426	29
AATAGGA	35	6.206097E-6	38.571426	4
AGCATAT	30	1.1344211E-4	37.500004	31
CGGTAGT	30	1.1344211E-4	37.500004	12
TGTACGG	30	1.1344211E-4	37.500004	2
GCATATG	30	1.1344211E-4	37.500004	32
TGCGGGA	85	0.0	37.058823	4
CCATTAT	85	0.0	37.058823	34
TACGGGG	55	2.7102942E-9	36.81818	4
GATTGGG	55	2.7102942E-9	36.81818	3
>>END_MODULE