##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551145_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	150376
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.965001063999573	33.0	31.0	34.0	30.0	34.0
2	32.08074426770229	33.0	31.0	34.0	30.0	34.0
3	31.986939405224238	33.0	31.0	34.0	30.0	34.0
4	35.75011304995478	37.0	35.0	37.0	35.0	37.0
5	35.81280922487631	37.0	35.0	37.0	35.0	37.0
6	35.78516518593393	37.0	35.0	37.0	35.0	37.0
7	26.80314677874129	35.0	10.0	37.0	0.0	37.0
8	31.122712400915038	35.0	19.0	37.0	17.0	37.0
9	35.85164520934192	39.0	32.0	39.0	32.0	39.0
10	36.64306804277278	38.0	35.0	39.0	33.0	39.0
11	37.06256982497207	39.0	37.0	39.0	34.0	39.0
12	37.111640155343935	39.0	37.0	39.0	34.0	39.0
13	37.077199819120075	39.0	37.0	39.0	33.0	39.0
14	38.28174708730116	40.0	38.0	41.0	34.0	41.0
15	38.342827312869076	40.0	38.0	41.0	34.0	41.0
16	38.29726153109539	40.0	37.0	41.0	34.0	41.0
17	38.19789062084375	40.0	37.0	41.0	33.0	41.0
18	38.084960366015856	40.0	37.0	41.0	33.0	41.0
19	37.977050859179656	40.0	37.0	41.0	33.0	41.0
20	38.04034553386179	40.0	37.0	41.0	34.0	41.0
21	37.98156620737352	40.0	37.0	41.0	33.0	41.0
22	37.97488296004681	40.0	37.0	41.0	33.0	41.0
23	37.92069878172049	40.0	37.0	41.0	33.0	41.0
24	37.855222907910836	40.0	36.0	41.0	33.0	41.0
25	37.75400329839868	40.0	36.0	41.0	33.0	41.0
26	37.55506197797521	40.0	36.0	41.0	33.0	41.0
27	37.44551657179337	39.0	36.0	41.0	33.0	41.0
28	37.43887987444805	39.0	36.0	41.0	33.0	41.0
29	37.33993456402617	39.0	36.0	41.0	32.0	41.0
30	37.298192530722986	39.0	36.0	41.0	32.0	41.0
31	37.19544342182263	39.0	36.0	41.0	32.0	41.0
32	37.0197837420865	39.0	35.0	41.0	31.0	41.0
33	36.95637601744959	39.0	35.0	41.0	31.0	41.0
34	36.76030084587966	39.0	35.0	41.0	30.0	41.0
35	36.595773261690695	39.0	35.0	41.0	30.0	41.0
36	36.398175240729906	39.0	35.0	41.0	30.0	41.0
37	36.38695004521998	39.0	35.0	41.0	30.0	41.0
38	36.201408469436615	39.0	35.0	40.0	30.0	41.0
39	36.103573708570515	39.0	35.0	40.0	29.0	41.0
40	35.787885034845985	39.0	35.0	40.0	27.0	41.0
41	35.59127121349152	38.0	35.0	40.0	26.0	41.0
42	35.50537319785072	38.0	35.0	40.0	26.0	41.0
43	35.43411182635527	38.0	35.0	40.0	26.0	41.0
44	35.206628717348515	38.0	34.0	40.0	25.0	41.0
45	35.07317657072937	38.0	34.0	40.0	24.0	41.0
46	34.81059477576209	38.0	34.0	40.0	23.0	41.0
47	34.638393094642765	38.0	34.0	40.0	23.0	41.0
48	34.45356306857477	38.0	33.0	40.0	23.0	41.0
49	34.22478986008406	37.0	33.0	40.0	22.0	41.0
50	34.04025243389903	37.0	33.0	40.0	21.0	41.0
51	31.653541788583283	35.0	29.0	39.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	14.0
10	11.0
11	12.0
12	6.0
13	6.0
14	9.0
15	8.0
16	14.0
17	25.0
18	54.0
19	117.0
20	192.0
21	286.0
22	434.0
23	734.0
24	930.0
25	1297.0
26	1715.0
27	2083.0
28	2325.0
29	2774.0
30	3458.0
31	4191.0
32	5158.0
33	6940.0
34	10297.0
35	12805.0
36	14315.0
37	22346.0
38	28131.0
39	29678.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.97693780922487	20.94350162259935	23.232430707027717	12.847129861148055
2	31.147257541096984	25.99151460339416	24.419455232217906	18.44177262329095
3	31.845507261797096	25.513379794648085	26.2575144969942	16.383598446560622
4	29.964888014044792	25.397669840932064	26.12318455072618	18.51425759429696
5	25.289274884290048	30.850667659732938	24.023780390487843	19.836277065489174
6	25.08977496409002	36.2424855030058	24.25254029898388	14.415199233920307
7	58.90434643826142	28.575703569718574	7.825051869979252	4.694898122040751
8	78.20330371867851	7.440682023727191	9.212241315103475	5.1437729424908225
9	71.52404639038144	9.095866361653455	11.594270362291855	7.7858168856732455
10	39.530244187902326	26.583364366654255	18.914587434165025	14.971804011278394
11	32.43536202585519	25.95959461616215	23.152630738947703	18.452412619034952
12	29.80927807628877	23.078815768473692	27.920013831994467	19.19189232324307
13	23.99917540032984	25.6071447571421	29.824573070170775	20.56910677235729
14	19.922727030909186	30.04801298079481	27.711203915518436	22.31805607277757
15	19.527052189179127	26.647204341118265	34.127121349151466	19.69862212055115
16	21.214156514337397	25.90040963983614	31.689897324041073	21.195536521785392
17	21.532026387189447	24.884290046283983	28.330318667872533	25.25336489865404
18	21.73684630526148	27.525668989732406	28.7319785072086	22.00550619779752
19	25.14363994254402	26.69441932223227	26.643879342448262	21.518061392775444
20	26.39849444060222	26.105894557642177	27.519683992126403	19.975927009629196
21	25.477469809012078	26.462334415066234	27.793663882534446	20.266531893387242
22	23.119380752247697	26.280789487684203	25.729504708198114	24.87032505186998
23	23.889450444219822	26.955099217960317	27.101399159440337	22.054051178379527
24	23.1187157525137	25.12102995158802	28.044368782252487	23.715885513645794
25	23.037585784965685	27.4910890035644	26.19899452040219	23.272330691067726
26	21.399026440389424	29.26331329467468	26.784859286056285	22.55280097887961
27	23.99119540352184	27.561578975368413	26.067989572804173	22.379236048305582
28	20.297786880885248	28.00513379794648	28.590333563866576	23.1067457573017
29	21.492791402883437	28.322338671064536	27.487764004894398	22.697105921157632
30	21.976911209235517	25.491434803426078	27.19516412193435	25.336489865404054
31	23.06352077459169	27.430574027770387	24.717375113049954	24.78853008458797
32	23.580225567909775	27.55027397989041	27.075464169814335	21.794036282385488
33	22.17441613023355	27.062164175134328	25.780709687716126	24.982710006915998
34	22.598020960791615	26.883279246688303	26.83805926477629	23.680640527743787
35	22.334016066393573	24.541815183273926	27.370724051710376	25.75344469862212
36	21.970261211895515	27.050194179922325	27.224424110230355	23.7551204979518
37	22.242911102835556	26.82874926850029	28.702053519178595	22.226286109485557
38	21.18223652710539	26.228254508698196	27.123344150662337	25.466164813534075
39	23.656700537319786	24.76658509336596	28.352928658828535	23.223785710485718
40	23.256370697451718	24.13483534606586	30.37984784806086	22.228946108421557
41	20.574426770229294	26.287439485024205	28.964728414108638	24.173405330637866
42	21.94432622226951	25.963584614566155	29.026573389370647	23.065515773793692
43	22.98505080597968	24.92352503058999	28.1208437516625	23.970580411767838
44	21.665691333723466	25.977549608980155	27.456509017396392	24.900250039899984
45	21.692956322817473	24.80981007607597	26.8606692557323	26.636564345374264
46	22.471006011597595	26.703729318508273	26.354604458158214	24.470660211735915
47	22.60334095866362	24.576395169441934	29.912353035058786	22.907910836835665
48	21.39104644358142	24.348300260679896	29.000638399744638	25.260014895994043
49	20.530536787785287	24.353620258551896	30.393812842474865	24.722030111187955
50	20.37825184869926	23.88280044687982	29.657658136936742	26.081289567484173
51	20.648241740703305	24.238575304569878	27.74910890035644	27.36407405437038
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	100.0
1	215.5
2	331.0
3	503.5
4	676.0
5	505.0
6	334.0
7	301.0
8	268.0
9	272.5
10	277.0
11	303.5
12	330.0
13	335.0
14	340.0
15	360.0
16	380.0
17	360.5
18	341.0
19	397.5
20	454.0
21	596.0
22	738.0
23	786.0
24	834.0
25	1025.5
26	1450.0
27	1683.0
28	2040.5
29	2398.0
30	2688.5
31	2979.0
32	3181.5
33	3384.0
34	3614.0
35	3844.0
36	4182.5
37	4521.0
38	5083.0
39	5645.0
40	6429.0
41	7213.0
42	7841.5
43	8470.0
44	9509.0
45	10548.0
46	11806.5
47	13065.0
48	13123.5
49	13182.0
50	12498.0
51	11814.0
52	10625.5
53	9437.0
54	8687.0
55	7937.0
56	7486.5
57	7036.0
58	6987.5
59	6939.0
60	6928.5
61	6918.0
62	6320.5
63	5723.0
64	4789.0
65	3855.0
66	3161.5
67	2468.0
68	2074.5
69	1681.0
70	1463.0
71	1245.0
72	995.5
73	746.0
74	609.0
75	350.0
76	228.0
77	183.5
78	139.0
79	112.0
80	85.0
81	62.0
82	39.0
83	31.0
84	23.0
85	18.0
86	13.0
87	11.0
88	9.0
89	10.0
90	11.0
91	7.0
92	3.0
93	2.5
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	150376.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.86234505506198
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.78218482015444	35.79228068308773
2	10.05054613835505	10.022875990849604
3	5.239994131846735	7.838351864659254
4	3.4288686467238363	6.838857264457094
5	2.4806284258678866	6.18449752620099
6	1.7591123084514741	5.2628078948768415
7	1.359010949440525	4.743443102622759
8	1.0415972046251716	4.154918338032665
9	0.7988690468251958	3.5850135659945734
>10	2.0218455342019976	13.367159653136138
>50	0.020005067950547472	0.643719742512103
>100	0.017337725557141144	1.5660743735702507
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTC	305	0.20282491887003246	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCG	301	0.20016491993403202	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	282	0.18752992498803	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGC	243	0.16159493536202585	No Hit
GCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	210	0.13964994414002235	TruSeq Adapter, Index 15 (95% over 21bp)
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	153	0.10174495930201628	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.649997340001063E-4	0.0	0.0	0.1542799382880247	0.0
2	6.649997340001063E-4	0.0	0.0	0.8418896632441347	0.0
3	6.649997340001063E-4	0.0	0.0	1.105894557642177	0.0
4	6.649997340001063E-4	0.0	0.0	1.418444432622227	0.0
5	6.649997340001063E-4	0.0	0.0	2.669973932010427	0.0
6	6.649997340001063E-4	0.0	0.0	3.2172687130925146	0.0
7	6.649997340001063E-4	0.0	0.0	3.8410384635846144	0.0
8	6.649997340001063E-4	0.0	0.0	4.918338032664787	0.0
9	6.649997340001063E-4	0.0	0.0	5.292732882906847	0.0
10	6.649997340001063E-4	0.0	0.0	6.49837740064904	0.0
11	6.649997340001063E-4	0.0	0.0	7.621561951375219	0.0
12	6.649997340001063E-4	0.0	0.0	8.781321487471406	0.0
13	6.649997340001063E-4	0.0	0.0	9.165026333989466	0.0
14	6.649997340001063E-4	0.0	0.0	9.357876256849497	0.0
15	6.649997340001063E-4	0.0	0.0	9.68705112517955	0.0
16	6.649997340001063E-4	0.0	0.0	10.31880087247965	0.0
17	6.649997340001063E-4	0.0	0.0	11.043650582539767	0.0
18	6.649997340001063E-4	0.0	0.0	11.838990264403895	0.0
19	6.649997340001063E-4	0.0	0.0	12.261930095227962	0.0
20	6.649997340001063E-4	0.0	0.0	12.726764909294037	0.0
21	6.649997340001063E-4	0.0	0.0	13.301324679470127	0.0
22	6.649997340001063E-4	0.0	0.0	13.87654944938022	0.0
23	6.649997340001063E-4	0.0	0.0	14.448449220620311	0.0
24	6.649997340001063E-4	0.0	0.0	14.943874022450391	0.0
25	6.649997340001063E-4	0.0	0.0	15.342208863116454	0.0
26	6.649997340001063E-4	0.0	0.0	15.717933712826515	0.0
27	6.649997340001063E-4	0.0	0.0	16.07437357025057	0.0
28	6.649997340001063E-4	0.0	0.0	16.42748842900463	0.0
29	6.649997340001063E-4	0.0	0.0	16.81850827259669	0.0
30	6.649997340001063E-4	0.0	0.0	17.280683087726764	0.0
31	6.649997340001063E-4	0.0	0.0	17.728892908442837	0.0
32	6.649997340001063E-4	0.0	0.0	18.152497739000903	0.0
33	6.649997340001063E-4	0.0	0.0	18.56479757408097	0.0
34	6.649997340001063E-4	0.0	0.0	18.92522742990903	0.0
35	6.649997340001063E-4	0.0	0.0	19.338192264723094	0.0
36	6.649997340001063E-4	0.0	0.0	19.763127094749162	0.0
37	6.649997340001063E-4	0.0	0.0	20.173431930627228	0.0
38	6.649997340001063E-4	0.0	0.0	20.56378677448529	0.0
39	6.649997340001063E-4	0.0	0.0	20.94483162206735	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGG	30	2.1528886E-6	45.000004	2
AACGTCC	20	7.013719E-4	45.0	21
ATGGGCG	20	7.013719E-4	45.0	5
ATCTATC	25	3.874595E-5	45.0	3
ATTAGGC	20	7.013719E-4	45.0	4
CACGGGA	20	7.013719E-4	45.0	4
CGAAAGG	25	3.874595E-5	45.0	3
CGCGGGA	25	3.874595E-5	45.0	4
TTAGCGG	20	7.013719E-4	45.0	2
GGGTCGT	20	7.013719E-4	45.0	7
CTTTTCG	25	3.874595E-5	45.0	26
TTTCGAC	20	7.013719E-4	45.0	24
AAGGTAT	25	3.874595E-5	45.0	6
TACGGGA	55	1.8189894E-12	45.0	4
ACGGGCT	20	7.013719E-4	45.0	5
ACGGGAC	25	3.874595E-5	45.0	5
TTACGGG	25	3.874595E-5	45.0	3
CAGTCTC	25	3.874595E-5	45.0	6
TTCGCCG	25	3.874595E-5	45.0	29
AAGGACC	20	7.013719E-4	45.0	6
>>END_MODULE