##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551144_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	160710
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.04675502457843	33.0	31.0	34.0	30.0	34.0
2	32.14295936780536	33.0	31.0	34.0	30.0	34.0
3	32.0269616078651	33.0	31.0	34.0	30.0	34.0
4	35.77721361458528	37.0	35.0	37.0	35.0	37.0
5	35.84831062161658	37.0	35.0	37.0	35.0	37.0
6	35.79545143426047	37.0	35.0	37.0	35.0	37.0
7	26.636936096073672	35.0	10.0	37.0	0.0	37.0
8	30.964239935287164	35.0	19.0	37.0	17.0	37.0
9	35.8025325119781	39.0	32.0	39.0	32.0	39.0
10	36.684419140065955	38.0	35.0	39.0	33.0	39.0
11	37.0382054632568	39.0	37.0	39.0	34.0	39.0
12	37.09629767904922	39.0	37.0	39.0	34.0	39.0
13	37.02110011822538	39.0	37.0	39.0	33.0	39.0
14	38.33175906913073	40.0	38.0	41.0	34.0	41.0
15	38.31393814946176	40.0	37.0	41.0	34.0	41.0
16	38.304243668720055	40.0	37.0	41.0	34.0	41.0
17	38.13717254682347	40.0	37.0	41.0	33.0	41.0
18	38.00899135088047	40.0	37.0	41.0	33.0	41.0
19	37.86473772633937	40.0	37.0	41.0	33.0	41.0
20	37.975807354862795	40.0	37.0	41.0	33.0	41.0
21	37.992956256611286	40.0	37.0	41.0	33.0	41.0
22	38.027565179515896	40.0	37.0	41.0	33.0	41.0
23	38.04025885134715	40.0	37.0	41.0	34.0	41.0
24	37.92232592869143	40.0	36.0	41.0	33.0	41.0
25	37.86410926513596	40.0	36.0	41.0	33.0	41.0
26	37.70989359716259	40.0	36.0	41.0	33.0	41.0
27	37.64830439922842	40.0	36.0	41.0	33.0	41.0
28	37.61887250326675	40.0	36.0	41.0	33.0	41.0
29	37.55634372472155	40.0	36.0	41.0	33.0	41.0
30	37.50830688818368	40.0	36.0	41.0	33.0	41.0
31	37.40189782838654	40.0	36.0	41.0	33.0	41.0
32	37.28650364009707	40.0	36.0	41.0	32.0	41.0
33	37.19489764171489	40.0	36.0	41.0	32.0	41.0
34	37.03088793478937	39.0	35.0	41.0	31.0	41.0
35	36.984916931118164	39.0	35.0	41.0	31.0	41.0
36	36.77261526974053	39.0	35.0	41.0	31.0	41.0
37	36.74437807230415	39.0	35.0	41.0	31.0	41.0
38	36.60153070748553	39.0	35.0	41.0	30.0	41.0
39	36.54683591562441	39.0	35.0	41.0	30.0	41.0
40	36.28483604007218	39.0	35.0	40.0	30.0	41.0
41	36.146512351440485	39.0	35.0	40.0	29.0	41.0
42	36.00942691805115	39.0	35.0	40.0	29.0	41.0
43	35.96328168751167	39.0	35.0	40.0	29.0	41.0
44	35.78799701325369	38.0	35.0	40.0	28.0	41.0
45	35.76737601891606	38.0	35.0	40.0	28.0	41.0
46	35.502936967208015	38.0	35.0	40.0	27.0	41.0
47	35.29015618194263	38.0	34.0	40.0	26.0	41.0
48	35.13704809906042	38.0	34.0	40.0	25.0	41.0
49	34.9227366063095	38.0	34.0	40.0	24.0	41.0
50	34.806265944869644	38.0	34.0	40.0	24.0	41.0
51	32.441353991662	35.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	16.0
10	16.0
11	12.0
12	10.0
13	6.0
14	5.0
15	12.0
16	19.0
17	48.0
18	70.0
19	100.0
20	160.0
21	277.0
22	405.0
23	623.0
24	869.0
25	1101.0
26	1501.0
27	1804.0
28	2160.0
29	2612.0
30	3320.0
31	4127.0
32	5507.0
33	7176.0
34	10900.0
35	13355.0
36	15060.0
37	23921.0
38	30903.0
39	34601.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.619438740588635	19.161844315848423	22.968079148777303	12.25063779478564
2	29.200423122394376	29.519009395806112	24.858440669528964	16.422126812270548
3	30.73113060792732	28.204218779167444	25.91811337191214	15.146537240993094
4	28.504760126936716	23.995395432767097	29.601144919420076	17.898699520876114
5	28.178706987741897	28.476137141434883	23.254931242610912	20.090224628212308
6	25.44147843942505	37.150146226121585	24.255491257544644	13.152884076908716
7	58.25399788438803	28.544583411113184	9.202289838840146	3.9991288656586397
8	77.46437682782653	8.878103416091095	9.256424615767532	4.401095140314853
9	71.13620807665983	8.935349387094767	10.10391388214797	9.824528654097442
10	40.46667911144297	26.77058054881464	18.21417460021156	14.548565739530833
11	32.0135648061726	25.429033663119903	24.374961110074047	18.18244042063344
12	30.688196129674566	22.458465559081574	27.402775185116045	19.450563126127808
13	24.254246779914133	24.575322008586895	30.82633314666169	20.344098064837286
14	20.48347955945492	28.77418953394313	27.37539667724473	23.366934229357227
15	19.563810590504634	26.708978906104164	34.684836040072184	19.04237446331902
16	22.503888992595357	25.598282620869888	30.00622238815257	21.89160599838218
17	22.070188538361023	25.078090971314793	29.75297119034285	23.098749299981332
18	22.109389583722233	28.034347582602205	28.288843258042434	21.567419575633128
19	24.290336631199054	28.3155995270985	25.649928442536247	21.7441353991662
20	26.344346960363385	26.802314728392755	28.586273411735423	18.26706489950843
21	24.96111007404642	28.432580424366872	26.95102980523925	19.655279696347456
22	23.060792732250636	24.93559828262087	26.808537116545327	25.195071868583163
23	24.110509613589695	28.252131167942256	27.53717876921162	20.100180449256424
24	24.22189036152075	24.439673946860804	27.689627278949665	23.64880841266878
25	22.188413913259907	30.191649555099247	25.50432455976604	22.115611971874806
26	21.878538983261777	27.828386534752035	27.246593242486465	23.04648123949972
27	23.89148155061913	28.553916993342042	26.052516956007715	21.502084500031113
28	20.881712401219588	27.69398295065646	29.85252940078402	21.57177524733993
29	23.33271109451808	25.987181880405704	29.136332524422876	21.543774500653353
30	21.820670773442846	26.88880592371352	30.090846867027565	21.199676435816066
31	26.052516956007715	25.675440233961794	26.015804865907537	22.256237944122955
32	24.824839773505072	28.44875863356356	27.46126563375023	19.265135959181134
33	23.878414535498724	28.178706987741897	25.63063903926327	22.31223943749611
34	23.096260344720303	26.408437558334892	28.580051023582854	21.915251073361958
35	22.490199738659697	26.93982950656462	27.83709787816564	22.732872876610045
36	24.598967083566674	29.001306701512043	26.164519942754026	20.235206272167257
37	22.626470039201045	28.60618505382366	29.48478626096696	19.282558646008336
38	23.98108394001618	28.97517267127123	24.96484350693796	22.078899881774625
39	24.269802750295565	25.14093709165578	28.127683404890796	22.46157675315786
40	22.91083317777363	28.063592806919296	29.175533569784086	19.850040445522993
41	21.57675315786199	28.34111131852405	27.37913011013627	22.70300541347769
42	21.289278825213117	27.722605936158295	29.308692676249144	21.67942256237944
43	25.338186796092337	25.582726650488457	26.25412233215108	22.824964221268125
44	22.76834048907971	26.231721734801816	28.006346835915625	22.99359094020285
45	21.8592495799888	25.88575695351876	27.17628025636239	25.07871321013005
46	23.39866840893535	29.0280629705681	25.622549934664924	21.95071868583162
47	21.776491817559577	26.072428598095946	31.202787629892352	20.94829195445212
48	23.50880467923589	25.626283367556468	27.525356231721737	23.339555721485905
49	20.85060046045672	25.767531578619874	30.399477319395185	22.98239064152822
50	20.392010453612098	25.868334266691555	29.60052268060482	24.139132599091532
51	21.109451807603758	25.38609918486715	28.067948478626096	25.436500528902993
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	90.0
1	236.5
2	383.0
3	773.0
4	1163.0
5	809.5
6	456.0
7	405.0
8	354.0
9	351.5
10	349.0
11	315.5
12	282.0
13	340.0
14	398.0
15	380.0
16	362.0
17	406.0
18	450.0
19	479.0
20	508.0
21	638.0
22	768.0
23	781.0
24	794.0
25	1071.0
26	1499.0
27	1650.0
28	2098.0
29	2546.0
30	2952.0
31	3358.0
32	3730.5
33	4103.0
34	4617.5
35	5132.0
36	5362.5
37	5593.0
38	6021.5
39	6450.0
40	7458.0
41	8466.0
42	9701.5
43	10937.0
44	14388.5
45	17840.0
46	15068.0
47	12296.0
48	11297.5
49	10299.0
50	9525.0
51	8751.0
52	8332.0
53	7913.0
54	7521.5
55	7130.0
56	7347.0
57	7564.0
58	7644.5
59	7725.0
60	7638.5
61	7552.0
62	6712.0
63	5872.0
64	4960.5
65	4049.0
66	3367.0
67	2685.0
68	2306.5
69	1928.0
70	1557.0
71	1186.0
72	1006.5
73	827.0
74	648.0
75	381.5
76	294.0
77	230.0
78	166.0
79	142.0
80	118.0
81	80.5
82	43.0
83	37.5
84	32.0
85	20.5
86	9.0
87	11.0
88	13.0
89	8.5
90	4.0
91	3.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	160710.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.98979528342978
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.51437139300668	29.25953580984382
2	11.43629059635623	10.061601642710473
3	6.4459092452189655	8.50662684338249
4	4.236449021161028	7.454421006782404
5	3.076553128889895	6.766847115923092
6	2.189657123458187	5.779354116109763
7	1.728527780921127	5.322630825710908
8	1.2094036437705105	4.2561134963599025
9	0.8628493832748669	3.4160910957625537
>10	2.237750367771868	12.827453176529152
>50	0.03394817245671608	1.0578059859374027
>100	0.02121760778544755	1.9588077904299668
>500	0.002829014371393007	0.629083442225126
>1k	0.00424352155708951	2.70362765229295
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCG	1692	1.0528280754153443	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGC	1437	0.8941571775247339	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTC	1216	0.7566423993528716	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTT	509	0.31671955696596354	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	502	0.3123638852591625	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCG	353	0.21965030178582542	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCT	313	0.19476074917553357	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGC	293	0.18231597287038764	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTC	270	0.16800448011946986	No Hit
GAATCTATCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTC	232	0.14435940513969261	No Hit
GAACTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCT	229	0.1424926886939207	No Hit
GCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC	223	0.13875925580237697	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	180	0.11200298674631323	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	175	0.10889179267002676	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	170	0.10578059859374027	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	163	0.10142492688693922	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06969074730881712	0.0
2	0.0	0.0	0.0	0.47787941011760315	0.0
3	0.0	0.0	0.0	0.5880156804181444	0.0
4	0.0	0.0	0.0	0.9377138945927447	0.0
5	0.0	0.0	0.0	2.5430900379565675	0.0
6	0.0	0.0	0.0	2.8560761620309876	0.0
7	0.0	0.0	0.0	3.3146661688756143	0.0
8	0.0	0.0	0.0	3.9729948354178335	0.0
9	0.0	0.0	0.0	4.147221703689876	0.0
10	0.0	0.0	0.0	6.00087113434136	0.0
11	0.0	0.0	0.0	6.501773380623483	0.0
12	0.0	0.0	0.0	8.100304897019477	0.0
13	0.0	0.0	0.0	8.33862236326302	0.0
14	0.0	0.0	0.0	8.474270424989111	0.0
15	0.0	0.0	0.0	8.738099682658204	0.0
16	0.0	0.0	0.0	9.042374463319021	0.0
17	0.0	0.0	0.0	9.372161035405389	0.0
18	0.0	0.0	0.0	9.72621492128679	0.0
19	0.0	0.0	0.0	9.94959865596416	0.0
20	0.0	0.0	0.0	10.172982390641529	0.0
21	0.0	0.0	0.0	10.459212245659884	0.0
22	0.0	0.0	0.0	10.777176280256363	0.0
23	0.0	0.0	0.0	11.087051210254495	0.0
24	0.0	0.0	0.0	11.343413602140501	0.0
25	0.0	0.0	0.0	11.5736419637857	0.0
26	0.0	0.0	0.0	11.76715823533072	0.0
27	0.0	0.0	0.0	11.961918984506253	0.0
28	0.0	0.0	0.0	12.190902868520938	0.0
29	0.0	0.0	0.0	12.440420633439114	0.0
30	0.0	0.0	0.0	12.724783772011698	0.0
31	0.0	0.0	0.0	12.98923526849605	0.0
32	0.0	0.0	0.0	13.242486466305769	0.0
33	0.0	0.0	0.0	13.467114678613651	0.0
34	0.0	0.0	0.0	13.725966025760687	0.0
35	0.0	0.0	0.0	13.990417522245037	0.0
36	0.0	0.0	0.0	14.266691556219277	0.0
37	0.0	0.0	0.0	14.548565739530831	0.0
38	0.0	0.0	0.0	14.802439176155808	0.0
39	0.0	0.0	0.0	15.056312612780784	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAATA	20	7.0151663E-4	45.0	20
TTGATCC	25	3.875794E-5	45.0	17
TTAGCAG	20	7.0151663E-4	45.0	2
ATAGCGG	20	7.0151663E-4	45.0	2
TATGCGG	25	3.875794E-5	45.0	2
CTGAAGC	40	6.7520887E-9	45.0	5
CAAGGTG	20	7.0151663E-4	45.0	5
CTACGAA	40	6.7520887E-9	45.0	11
TACGAAT	40	6.7520887E-9	45.0	12
TCTAACT	35	1.2032251E-7	45.0	24
TCGTCCC	35	1.2032251E-7	45.0	38
TAGGGTC	20	7.0151663E-4	45.0	5
CGAATGG	20	7.0151663E-4	45.0	2
CGAATAT	40	6.7520887E-9	45.0	14
TCATAGG	20	7.0151663E-4	45.0	3
GCTACGA	40	6.7520887E-9	45.0	10
TAGTGAC	20	7.0151663E-4	45.0	44
TAATGCG	20	7.0151663E-4	45.0	1
TCGCGGG	20	7.0151663E-4	45.0	3
TATGGGA	75	0.0	42.0	4
>>END_MODULE