FastQCFastQC Report
Sat 18 Jun 2016
SRR3551142_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551142_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92417
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTC4110.44472337340532586No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGC3520.3808823052035881No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCG3240.3505848491078481No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2930.3170412370018503No Hit
GCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC1720.1861129445881169No Hit
CCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC980.10604109633508987No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCT940.10171288832141272No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCC302.1438227E-645.00000419
CTCGTAA302.1438227E-645.00000433
TAAAACG302.1438227E-645.00000421
CGAATAG302.1438227E-645.00000426
AACGAAT302.1438227E-645.00000424
TCGTAAC302.1438227E-645.00000434
CGTAACC302.1438227E-645.00000435
GCTCGTA302.1438227E-645.00000432
TCTATCT302.1438227E-645.0000044
TTGATGG206.9996226E-445.02
CGGGTAT206.9996226E-445.06
AGTCACC253.862928E-545.017
CGTGCGG206.9996226E-445.02
CCAATTA206.9996226E-445.025
ACGGGCT206.9996226E-445.05
AATGACT206.9996226E-445.02
GATGGGC206.9996226E-445.04
CTACGGG206.9996226E-445.03
TCCAATT206.9996226E-445.024
CAAGCTT453.765308E-1045.022