##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551142_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	92417
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.952649404330373	33.0	31.0	34.0	30.0	34.0
2	32.0839888765054	33.0	31.0	34.0	30.0	34.0
3	31.953276994492356	33.0	31.0	34.0	30.0	34.0
4	35.707185907354706	37.0	35.0	37.0	35.0	37.0
5	35.79291688758562	37.0	35.0	37.0	35.0	37.0
6	35.752469783697805	37.0	35.0	37.0	35.0	37.0
7	26.77694580001515	35.0	10.0	37.0	0.0	37.0
8	31.067011480571757	35.0	19.0	37.0	17.0	37.0
9	35.82559485808888	39.0	32.0	39.0	32.0	39.0
10	36.63639806529102	38.0	35.0	39.0	33.0	39.0
11	36.99450317582263	39.0	37.0	39.0	34.0	39.0
12	37.069976303061125	39.0	37.0	39.0	34.0	39.0
13	37.04015494984689	39.0	37.0	39.0	33.0	39.0
14	38.23731564538992	40.0	37.0	41.0	33.0	41.0
15	38.303991689840615	40.0	38.0	41.0	34.0	41.0
16	38.26900894857007	40.0	37.0	41.0	34.0	41.0
17	38.14790568834738	40.0	37.0	41.0	33.0	41.0
18	38.026683402404316	40.0	37.0	41.0	33.0	41.0
19	37.922070614713746	40.0	37.0	41.0	33.0	41.0
20	37.97673588192649	40.0	37.0	41.0	33.0	41.0
21	37.938409599965375	40.0	37.0	41.0	33.0	41.0
22	37.943397859701136	40.0	37.0	41.0	33.0	41.0
23	37.91382537844769	40.0	36.0	41.0	33.0	41.0
24	37.869277297466915	40.0	36.0	41.0	33.0	41.0
25	37.76021727604228	40.0	36.0	41.0	33.0	41.0
26	37.62042697772055	40.0	36.0	41.0	33.0	41.0
27	37.475161496261514	39.0	36.0	41.0	33.0	41.0
28	37.330696733285	39.0	36.0	41.0	32.0	41.0
29	37.321358624495495	39.0	36.0	41.0	32.0	41.0
30	37.244944112014025	39.0	36.0	41.0	32.0	41.0
31	37.02911801941201	39.0	35.0	41.0	31.0	41.0
32	36.87481740372442	39.0	35.0	41.0	31.0	41.0
33	36.785374985121784	39.0	35.0	41.0	31.0	41.0
34	36.66602464914464	39.0	35.0	41.0	30.0	41.0
35	36.57245961240897	39.0	35.0	41.0	30.0	41.0
36	36.32161831697631	39.0	35.0	41.0	30.0	41.0
37	36.20835993377842	39.0	35.0	41.0	30.0	41.0
38	36.01665278033262	39.0	35.0	40.0	29.0	41.0
39	36.0700304056613	39.0	35.0	40.0	29.0	41.0
40	35.93739247108216	39.0	35.0	40.0	28.0	41.0
41	35.8406029193763	38.0	35.0	40.0	28.0	41.0
42	35.71428416849714	38.0	35.0	40.0	27.0	41.0
43	35.65204453726046	38.0	35.0	40.0	27.0	41.0
44	35.46495774586926	38.0	34.0	40.0	27.0	41.0
45	35.38513476957703	38.0	34.0	40.0	26.0	41.0
46	35.22474220111018	38.0	34.0	40.0	26.0	41.0
47	35.04864905807373	38.0	34.0	40.0	25.0	41.0
48	34.95672874038326	38.0	34.0	40.0	25.0	41.0
49	34.73270069359533	38.0	34.0	40.0	24.0	41.0
50	34.52305311793285	37.0	33.0	40.0	23.0	41.0
51	32.27232002770053	35.0	30.0	39.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	7.0
10	11.0
11	4.0
12	2.0
13	6.0
14	8.0
15	5.0
16	9.0
17	19.0
18	36.0
19	65.0
20	111.0
21	180.0
22	253.0
23	429.0
24	618.0
25	800.0
26	1082.0
27	1162.0
28	1378.0
29	1607.0
30	1888.0
31	2433.0
32	3134.0
33	4212.0
34	6345.0
35	8150.0
36	8971.0
37	13793.0
38	17401.0
39	18292.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.963058744603266	20.21273142387223	24.517134293474143	13.30707553805036
2	31.650021100014065	25.57970936083188	25.68575045716697	17.08451908198708
3	31.585097979808907	25.4293041323566	27.216854042005256	15.768743845829231
4	28.60188060638194	25.2724065918608	27.986193016436374	18.139519785320882
5	25.83507363363883	29.45994784509344	24.533365073525434	20.1716134477423
6	25.62731964898233	34.921064306350566	25.127411623402622	14.324204421264486
7	59.56371663222134	28.17014185701765	7.902225780971034	4.363915729789974
8	79.40530421892076	6.973825162037288	9.04162654057154	4.579244078470411
9	72.97466916259995	8.642349351309823	11.007715030784379	7.375266455305842
10	40.35188331151195	27.86175703604315	17.93392990467122	13.852429747773678
11	30.84822056548038	25.395760520250604	25.1317398314163	18.62427908285272
12	30.151379075278356	21.398660419619766	28.82045511107264	19.629505394029238
13	24.20442126448597	24.030210891935468	30.320179187811767	21.445188655766795
14	19.20317690468204	28.72415248276832	28.58023956631356	23.49243104623608
15	18.671889371003168	26.450761223584408	34.61376153737948	20.263587868032936
16	20.815434389776772	25.304868151963383	32.14235476156984	21.737342696690003
17	20.751593321575037	24.853652466537543	29.532445329322528	24.862308882564896
18	21.45600917580099	27.06320265751972	29.16671175216681	22.31407641451248
19	24.71406775809645	27.745977471677293	25.652206845060977	21.887747925165282
20	26.32524319118777	26.334981659218542	27.45165932674724	19.888115822846448
21	24.878539662616188	27.269874590172805	27.665905623424266	20.18568012378675
22	22.637609963534846	25.288637371912092	26.824069164764058	25.249683499789
23	22.520748347165565	27.573931203133622	27.236330978066807	22.668989471634006
24	23.310646309661642	24.470606057327117	28.446065117889567	23.772682515121677
25	22.582425311360463	27.175736065875327	26.45833558760834	23.78350303515587
26	22.448250862936472	28.165813649003972	26.025514786240628	23.360420701818928
27	23.759697891080645	28.205849573130486	26.478894575673305	21.55555796011556
28	20.980988346299924	28.00025969248082	29.283573368536093	21.735178592683162
29	24.1330058322603	26.54922795589556	27.79034160381748	21.527424608026664
30	23.90361080753541	26.21703799084584	28.388716361708344	21.490634839910406
31	24.17412380839023	26.68340240431955	26.59467414003917	22.547799647251047
32	25.866453141737995	28.249131653267256	25.335165608059125	20.549249596935628
33	23.839769739333672	28.099808476795396	26.442104807557048	21.61831697631388
34	23.04121536081024	26.157525130657778	29.021716783708623	21.779542724823354
35	22.711189499767357	25.516950344633564	27.15625912981378	24.6156010257853
36	23.911185171559346	28.225326509192033	26.963653873205146	20.899834446043478
37	23.263036021511194	28.351926593592086	28.480690781998984	19.904346602897736
38	21.60857850828311	27.710269755564454	27.27312074618306	23.40803098996938
39	22.856184468225543	26.469156107642533	28.807470487031605	21.86718893710032
40	23.506497722280535	24.36348290898861	30.941277037774434	21.188742330956426
41	20.03311079130463	27.216854042005256	29.366891372799376	23.383143793890735
42	23.215425733360746	25.87078134975167	27.984028912429533	22.929764004458054
43	23.395046365928344	27.16816170185139	27.375915686507895	22.060876245712368
44	22.410379042816796	26.41505350747157	28.0662648646894	23.108302585022237
45	21.907224861226833	25.59918629689343	26.820823008753802	25.672765833125936
46	22.756635683910968	27.553372215068656	26.624971596134912	23.065020504885464
47	21.872599197117413	26.26681238300313	29.675276193773875	22.185312226105587
48	22.159342978023524	25.024616683077788	28.42334202581776	24.392698313080928
49	20.567644480993756	25.69765302920458	30.017204626854365	23.717497862947294
50	20.15430061568759	25.756083837389227	29.54326584935672	24.546349697566466
51	20.27440838806713	25.008385903026497	27.976454548405595	26.740751160500775
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	57.0
1	124.0
2	191.0
3	428.0
4	665.0
5	474.0
6	283.0
7	264.5
8	246.0
9	238.0
10	230.0
11	229.5
12	229.0
13	235.5
14	242.0
15	249.5
16	257.0
17	248.5
18	240.0
19	258.5
20	277.0
21	370.5
22	464.0
23	474.0
24	484.0
25	621.0
26	886.5
27	1015.0
28	1237.0
29	1459.0
30	1668.5
31	1878.0
32	2011.5
33	2145.0
34	2438.5
35	2732.0
36	2780.5
37	2829.0
38	3134.0
39	3439.0
40	3966.0
41	4493.0
42	5157.0
43	5821.0
44	7278.0
45	8735.0
46	8387.0
47	8039.0
48	7740.5
49	7442.0
50	6495.0
51	5548.0
52	5037.5
53	4527.0
54	4372.0
55	4217.0
56	4247.0
57	4277.0
58	4350.0
59	4423.0
60	4243.5
61	4064.0
62	3739.0
63	3414.0
64	2844.5
65	2275.0
66	1948.0
67	1621.0
68	1349.5
69	1078.0
70	930.0
71	782.0
72	674.0
73	566.0
74	474.5
75	306.0
76	229.0
77	203.0
78	177.0
79	133.5
80	90.0
81	68.0
82	46.0
83	29.0
84	12.0
85	13.0
86	14.0
87	13.0
88	12.0
89	9.0
90	6.0
91	5.0
92	4.0
93	2.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	92417.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.97887834489326
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.16016546178528	39.834662453877534
2	12.262728573085397	13.72907581938388
3	6.22414659604902	10.452622353030288
4	3.86979549232613	8.665072443381629
5	2.4316696949781575	6.8061071015072985
6	1.5888970502957436	5.336680480863911
7	1.0206054045695288	3.999264204637675
8	0.5663586809448332	2.536329896014802
9	0.3228051184907411	1.6263241611391845
>10	0.512235667066146	4.051202700801801
>50	0.03092743650210693	1.2833136760552712
>100	0.009664823906908416	1.6793447093067293
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTC	411	0.44472337340532586	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGC	352	0.3808823052035881	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCG	324	0.3505848491078481	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	293	0.3170412370018503	No Hit
GCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	172	0.1861129445881169	No Hit
CCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	98	0.10604109633508987	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCT	94	0.10171288832141272	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1374206044342491	0.0
2	0.0	0.0	0.0	0.7109081662464698	0.0
3	0.0	0.0	0.0	0.9338108789508424	0.0
4	0.0	0.0	0.0	1.2508521159526926	0.0
5	0.0	0.0	0.0	2.4551759957583563	0.0
6	0.0	0.0	0.0	3.0351558695910925	0.0
7	0.0	0.0	0.0	3.6292024194682795	0.0
8	0.0	0.0	0.0	4.722074942921757	0.0
9	0.0	0.0	0.0	5.166798316327083	0.0
10	0.0	0.0	0.0	6.503132540549899	0.0
11	0.0	0.0	0.0	7.54514861984267	0.0
12	0.0	0.0	0.0	8.922600820195418	0.0
13	0.0	0.0	0.0	9.346765205535778	0.0
14	0.0	0.0	0.0	9.561011502212796	0.0
15	0.0	0.0	0.0	9.84450912710865	0.0
16	0.0	0.0	0.0	10.347663308698616	0.0
17	0.0	0.0	0.0	10.902755986452709	0.0
18	0.0	0.0	0.0	11.495720484326476	0.0
19	0.0	0.0	0.0	11.846305333434325	0.0
20	0.0	0.0	0.0	12.240172262678945	0.0
21	0.0	0.0	0.0	12.68381358408085	0.0
22	0.0	0.0	0.0	13.158834413581916	0.0
23	0.0	0.0	0.0	13.585162902929115	0.0
24	0.0	0.0	0.0	13.963881104125864	0.0
25	0.0	0.0	0.0	14.270101821093522	0.0
26	0.0	0.0	0.0	14.549271237975697	0.0
27	0.0	0.0	0.0	14.870640682991224	0.0
28	0.0	0.0	0.0	15.151974203880238	0.0
29	0.0	0.0	0.0	15.489574428947055	0.0
30	0.0	0.0	0.0	15.837995174048064	0.0
31	0.0	0.0	0.0	16.16369282707727	0.0
32	0.0	0.0	0.0	16.52726230022615	0.0
33	0.0	0.0	0.0	16.807513769111743	0.0
34	0.0	0.0	0.0	17.124555006113592	0.0
35	0.0	0.0	0.0	17.4783860112317	0.0
36	0.0	0.0	0.0	17.803001612257486	0.0
37	0.0	0.0	0.0	18.1406018373243	0.0
38	0.0	0.0	0.0	18.439248190268025	0.0
39	0.0	0.0	0.0	18.745468907235683	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACCCC	30	2.1438227E-6	45.000004	19
CTCGTAA	30	2.1438227E-6	45.000004	33
TAAAACG	30	2.1438227E-6	45.000004	21
CGAATAG	30	2.1438227E-6	45.000004	26
AACGAAT	30	2.1438227E-6	45.000004	24
TCGTAAC	30	2.1438227E-6	45.000004	34
CGTAACC	30	2.1438227E-6	45.000004	35
GCTCGTA	30	2.1438227E-6	45.000004	32
TCTATCT	30	2.1438227E-6	45.000004	4
TTGATGG	20	6.9996226E-4	45.0	2
CGGGTAT	20	6.9996226E-4	45.0	6
AGTCACC	25	3.862928E-5	45.0	17
CGTGCGG	20	6.9996226E-4	45.0	2
CCAATTA	20	6.9996226E-4	45.0	25
ACGGGCT	20	6.9996226E-4	45.0	5
AATGACT	20	6.9996226E-4	45.0	2
GATGGGC	20	6.9996226E-4	45.0	4
CTACGGG	20	6.9996226E-4	45.0	3
TCCAATT	20	6.9996226E-4	45.0	24
CAAGCTT	45	3.765308E-10	45.0	22
>>END_MODULE