FastQCFastQC Report
Sat 18 Jun 2016
SRR3551141_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551141_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences201051
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6700.33324877767332667No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT3550.1765721135433298No Hit
GAATCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTC3390.16861393377799663No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCG3240.1611531402479968No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGC3190.15866620907133017No Hit
GCCAGACAAAGGTTGCCAATCTTTGTGGTATAAACCAGAAGCTAATGGCTG2160.10743542683199785No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGGC302.156541E-645.0000044
ATAGGAT253.879293E-545.05
AGGAACG253.879293E-545.07
AACGGGA253.879293E-545.04
CCCCATT551.8189894E-1245.042
GTATCCT207.019393E-445.012
GTAAGGC207.019393E-445.04
GTAAGCA207.019393E-445.01
GCGCTTT207.019393E-445.040
GATTCAT551.8189894E-1245.045
TAAACCA650.041.5384631
ACGGGAG650.041.538465
CGAATAT650.041.5384614
CTCGTCC603.6379788E-1241.25000437
AGGCGAT603.6379788E-1241.2500047
TGATTCA603.6379788E-1241.25000444
TAGGGCG556.002665E-1140.9090925
TGCCAAT501.0713848E-940.514
TAATGGC501.0713848E-940.543
GGTATGC451.9146682E-840.08