##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551141_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	201051
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.145420813624405	33.0	31.0	34.0	30.0	34.0
2	32.27356740329568	33.0	31.0	34.0	30.0	34.0
3	32.14787790162695	33.0	31.0	34.0	30.0	34.0
4	35.881716579375386	37.0	35.0	37.0	35.0	37.0
5	35.946202704786344	37.0	35.0	37.0	35.0	37.0
6	35.93527015533372	37.0	35.0	37.0	35.0	37.0
7	26.696460102163133	36.0	10.0	37.0	0.0	37.0
8	31.099691123147856	36.0	19.0	37.0	17.0	37.0
9	35.97355894772968	39.0	32.0	39.0	32.0	39.0
10	36.82478077701678	38.0	35.0	39.0	34.0	39.0
11	37.18549522260521	39.0	37.0	39.0	34.0	39.0
12	37.196189026664875	39.0	37.0	39.0	34.0	39.0
13	37.13357804736112	39.0	37.0	39.0	34.0	39.0
14	38.37139830192339	40.0	38.0	41.0	34.0	41.0
15	38.44547900781394	40.0	38.0	41.0	34.0	41.0
16	38.36463882298521	40.0	37.0	41.0	34.0	41.0
17	38.22856887058508	40.0	37.0	41.0	34.0	41.0
18	38.15059363047187	40.0	37.0	41.0	34.0	41.0
19	38.03117616923069	40.0	37.0	41.0	34.0	41.0
20	38.04672446294721	40.0	37.0	41.0	34.0	41.0
21	37.97931868033484	40.0	36.0	41.0	33.0	41.0
22	37.96701831873505	40.0	36.0	41.0	33.0	41.0
23	37.92206952464797	40.0	36.0	41.0	33.0	41.0
24	37.86063735072196	40.0	36.0	41.0	33.0	41.0
25	37.763234204256634	40.0	36.0	41.0	33.0	41.0
26	37.62036995588184	40.0	36.0	41.0	33.0	41.0
27	37.4800473511696	40.0	35.0	41.0	33.0	41.0
28	37.39370110071574	40.0	35.0	41.0	33.0	41.0
29	37.31659628651437	40.0	35.0	41.0	32.0	41.0
30	37.20675848416571	39.0	35.0	41.0	32.0	41.0
31	37.11592083600679	39.0	35.0	41.0	32.0	41.0
32	36.965665428174944	39.0	35.0	41.0	31.0	41.0
33	36.850416063585854	39.0	35.0	41.0	31.0	41.0
34	36.69896692878921	39.0	35.0	41.0	31.0	41.0
35	36.60054414054145	39.0	35.0	41.0	30.0	41.0
36	36.42390239292518	39.0	35.0	41.0	30.0	41.0
37	36.32747909734346	39.0	35.0	41.0	30.0	41.0
38	36.23213512989241	39.0	35.0	41.0	30.0	41.0
39	36.14304330741951	39.0	35.0	41.0	29.0	41.0
40	35.95191767263033	39.0	35.0	40.0	29.0	41.0
41	35.83073946411607	38.0	35.0	40.0	28.0	41.0
42	35.762970589551905	38.0	35.0	40.0	28.0	41.0
43	35.70763139700872	38.0	35.0	40.0	28.0	41.0
44	35.52068878045869	38.0	35.0	40.0	27.0	41.0
45	35.40815514471453	38.0	34.0	40.0	27.0	41.0
46	35.28208265564459	38.0	34.0	40.0	26.0	41.0
47	35.1519763642061	38.0	34.0	40.0	26.0	41.0
48	35.08088494958991	38.0	34.0	40.0	26.0	41.0
49	34.90671521156324	37.0	34.0	40.0	25.0	41.0
50	34.72865093931391	37.0	34.0	40.0	24.0	41.0
51	32.6252343932634	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	14.0
9	25.0
10	21.0
11	11.0
12	5.0
13	5.0
14	15.0
15	21.0
16	32.0
17	61.0
18	98.0
19	158.0
20	290.0
21	462.0
22	684.0
23	926.0
24	1289.0
25	1703.0
26	2068.0
27	2443.0
28	2710.0
29	3189.0
30	3901.0
31	4861.0
32	6200.0
33	8861.0
34	14274.0
35	17871.0
36	18521.0
37	29555.0
38	38148.0
39	42620.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.113185211712455	19.784034896618273	23.636788675510196	12.465991216159084
2	32.136124664886026	25.533819777071486	24.532083899110177	17.797971658932312
3	33.17168280685001	24.090902308369518	26.429115000671473	16.308299884109008
4	29.799404131290068	25.220466448811496	26.19086699394681	18.789262425951627
5	25.88397968674615	30.204276526851398	24.075483335074185	19.83626045132827
6	26.720085948341467	36.094324325668616	23.600976866566196	13.584612859423729
7	60.29564637828213	28.886203003218093	6.808720175477864	4.00943044302192
8	81.6932022223217	6.525707407573203	7.936294770978508	3.8447955991265896
9	75.05060904944516	8.857454078815822	9.969112314785802	6.122824556953211
10	43.87742413616446	26.009321018050148	16.37445225340834	13.73880259237706
11	33.84365161078532	26.065525662642813	22.594764512486883	17.496058214084982
12	33.10254612013867	22.174970529865558	26.191861766417475	18.530621583578295
13	26.753410826108798	23.95312632118219	27.11550800543146	22.177954847277555
14	20.762891007754252	26.990166674127458	28.71062566214543	23.536316655972865
15	21.88201003725423	25.302535177641495	32.27837712819135	20.53707765691292
16	24.922034707611502	25.102088524802163	29.72330403728407	20.252572730302262
17	23.773072503991525	24.71959850983084	27.693470810888783	23.813858175288857
18	24.66886511382684	25.80738220650482	27.997373800677437	21.526378878990904
19	27.519385628522112	26.583802119860135	25.0220093409135	20.874802910704247
20	29.098089539470084	25.853141740155483	26.09984531288081	18.94892340749362
21	27.39752600086545	26.559927580564135	26.109793037587476	19.932753380982934
22	25.35127902870416	24.617136945352176	26.641498923158803	23.390085102784866
23	25.769083466384153	25.912330702160148	27.44079860333945	20.877787228116247
24	25.668611446846818	23.578594485976197	28.152060919866102	22.60073314731088
25	24.707163853947506	26.137646666766145	25.605940781194818	23.549248698091528
26	22.771336626030212	27.689491721006114	26.5251105440908	23.014061108872873
27	23.194612312298872	27.401505090748117	27.059800747074124	22.344081849878886
28	21.786014493834898	28.984188091578755	27.39454168345345	21.835255731132897
29	23.90537724259019	27.51839085605145	26.982208494362126	21.594023406996236
30	23.939199506592853	26.208275512183477	28.157532168454768	21.6949928127689
31	25.049862970092168	26.918045670004126	26.26249061183481	21.7696007480689
32	26.095866222998144	27.413442360396118	25.466175249066158	21.02451616753958
33	26.136154508060145	26.872286136353463	25.047873425150836	21.94368593043556
34	24.205301142496182	27.61538117194145	27.189618554496125	20.989699131066246
35	23.618385384802863	26.612153135274134	27.06328245072146	22.706179029201547
36	23.86956543364619	29.90087092329807	25.020517182207502	21.209046460848242
37	24.032210732600188	29.51639136338541	26.7429657151668	19.708432188847606
38	24.362972579096844	28.179417162809433	24.957846516555502	22.499763741538217
39	25.442798096005493	27.231398998264122	24.926511183729502	22.399291722000886
40	25.596987828958824	25.622851913196154	26.920035214945457	21.860125042899565
41	21.689521564180232	26.700190498928134	27.591506632645448	24.018781304246186
42	23.07523961581887	26.42264897961214	26.378878990902805	24.123232413666184
43	24.111792530253517	26.233144823950145	26.464429423380135	23.190633222416203
44	22.71264505026088	26.817573650466798	26.55594849068147	23.913832808590854
45	23.217989465359537	27.02448632436546	25.470154338948824	24.28736987132618
46	22.32916026281889	28.83397744850809	25.233895877165498	23.602966411507527
47	22.394815245882885	26.118248603588146	28.136641946570773	23.3502942039582
48	23.251811729362203	25.040412631620836	28.553451611780094	23.154324027236868
49	22.900657047216875	26.269454019129473	28.028709133503437	22.801179800150212
50	21.092658081780243	26.276417426424143	27.51242222122745	25.118502270568165
51	21.27917792003024	25.61190941601882	27.06228767825079	26.046624985700145
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	117.0
1	316.0
2	515.0
3	1029.5
4	1544.0
5	1101.5
6	659.0
7	605.5
8	552.0
9	520.0
10	488.0
11	498.5
12	509.0
13	497.5
14	486.0
15	497.5
16	509.0
17	504.0
18	499.0
19	489.0
20	479.0
21	639.5
22	800.0
23	888.0
24	976.0
25	1173.0
26	1766.0
27	2162.0
28	2444.5
29	2727.0
30	3255.0
31	3783.0
32	4287.0
33	4791.0
34	5371.0
35	5951.0
36	6091.0
37	6231.0
38	6653.0
39	7075.0
40	8197.5
41	9320.0
42	10456.0
43	11592.0
44	13109.5
45	14627.0
46	14112.5
47	13598.0
48	13504.5
49	13411.0
50	12440.0
51	11469.0
52	10985.5
53	10502.0
54	10442.5
55	10383.0
56	10652.0
57	10921.0
58	11052.0
59	11183.0
60	11090.0
61	10997.0
62	10152.0
63	9307.0
64	7949.5
65	6592.0
66	5460.0
67	4328.0
68	3786.0
69	3244.0
70	2851.5
71	2459.0
72	2168.0
73	1877.0
74	1543.5
75	989.5
76	769.0
77	594.0
78	419.0
79	360.5
80	302.0
81	223.5
82	145.0
83	113.0
84	81.0
85	60.0
86	39.0
87	30.0
88	21.0
89	18.5
90	16.0
91	12.5
92	9.0
93	6.5
94	4.0
95	3.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	201051.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.75010320764383
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.53641334778827	27.77902124336611
2	9.948891456891314	8.307344902537167
3	5.744647899069562	7.1951892803318565
4	4.110127592656572	6.863930047599863
5	3.0891480718140554	6.448612541096538
6	2.3314549851678	5.840309175283883
7	1.8203695540809397	5.320043173125226
8	1.4998987359868476	5.009674162277234
9	1.1389223126317922	4.279511168807915
>10	3.728898366671035	20.36000815713426
>50	0.033357557273734496	0.9082272657186484
>100	0.016678778636867248	1.3548801050479728
>500	0.0011913413312048034	0.33324877767332667
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	670	0.33324877767332667	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	355	0.1765721135433298	No Hit
GAATCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTC	339	0.16861393377799663	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCG	324	0.1611531402479968	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGC	319	0.15866620907133017	No Hit
GCCAGACAAAGGTTGCCAATCTTTGTGGTATAAACCAGAAGCTAATGGCTG	216	0.10743542683199785	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06217327941666542	0.0
2	0.0	0.0	0.0	0.25814345613799483	0.0
3	0.0	0.0	0.0	0.3382226400266599	0.0
4	0.0	0.0	0.0	0.45660056403599086	0.0
5	0.0	0.0	0.0	0.9306096463086481	0.0
6	0.0	0.0	0.0	1.2111354830366425	0.0
7	0.0	0.0	0.0	1.4245141779946382	0.0
8	0.0	0.0	0.0	1.8204336213199637	0.0
9	0.0	0.0	0.0	1.9795972166266271	0.0
10	0.0	0.0	0.0	2.556565249613282	0.0
11	0.0	0.0	0.0	3.094737156243938	0.0
12	0.0	0.0	0.0	3.723930743940592	0.0
13	0.0	0.0	0.0	3.9596918194885875	0.0
14	0.0	0.0	0.0	4.080556674674585	0.0
15	0.0	0.0	0.0	4.262102650571248	0.0
16	0.0	0.0	0.0	4.568989957771908	0.0
17	0.0	0.0	0.0	4.8957727143859024	0.0
18	0.0	0.0	0.0	5.250409100178562	0.0
19	0.0	0.0	0.0	5.491641424315223	0.0
20	0.0	0.0	0.0	5.740334541981885	0.0
21	0.0	0.0	0.0	6.013896971415213	0.0
22	0.0	0.0	0.0	6.306360077791207	0.0
23	0.0	0.0	0.0	6.602802274049868	0.0
24	0.0	0.0	0.0	6.84851107430453	0.0
25	0.0	0.0	0.0	7.068355790321859	0.0
26	0.0	0.0	0.0	7.270791988102522	0.0
27	0.0	0.0	0.0	7.497600111414517	0.0
28	0.0	0.0	0.0	7.736345504374512	0.0
29	0.0	0.0	0.0	7.961164082745174	0.0
30	0.0	0.0	0.0	8.261087982651167	0.0
31	0.0	0.0	0.0	8.525697459848496	0.0
32	0.0	0.0	0.0	8.779364439868491	0.0
33	0.0	0.0	0.0	9.01711506035782	0.0
34	0.0	0.0	0.0	9.289682717320481	0.0
35	0.0	0.0	0.0	9.53638629004581	0.0
36	0.0	0.0	0.0	9.80298531218447	0.0
37	0.0	0.0	0.0	10.069086948087799	0.0
38	0.0	0.0	0.0	10.405320043173125	0.0
39	0.0	0.0	0.0	10.70375178437312	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGGGC	30	2.156541E-6	45.000004	4
ATAGGAT	25	3.879293E-5	45.0	5
AGGAACG	25	3.879293E-5	45.0	7
AACGGGA	25	3.879293E-5	45.0	4
CCCCATT	55	1.8189894E-12	45.0	42
GTATCCT	20	7.019393E-4	45.0	12
GTAAGGC	20	7.019393E-4	45.0	4
GTAAGCA	20	7.019393E-4	45.0	1
GCGCTTT	20	7.019393E-4	45.0	40
GATTCAT	55	1.8189894E-12	45.0	45
TAAACCA	65	0.0	41.53846	31
ACGGGAG	65	0.0	41.53846	5
CGAATAT	65	0.0	41.53846	14
CTCGTCC	60	3.6379788E-12	41.250004	37
AGGCGAT	60	3.6379788E-12	41.250004	7
TGATTCA	60	3.6379788E-12	41.250004	44
TAGGGCG	55	6.002665E-11	40.909092	5
TGCCAAT	50	1.0713848E-9	40.5	14
TAATGGC	50	1.0713848E-9	40.5	43
GGTATGC	45	1.9146682E-8	40.0	8
>>END_MODULE