FastQCFastQC Report
Sat 18 Jun 2016
SRR3551140_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551140_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences471419
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6370.13512395554697626No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG6260.1327905748389437No Hit
GAGGAGCTGAGAACCCCAGTCTAATAATGTCCATCGACACCTCCTTATCCC5130.10882039120188199No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT5020.10648701049384941No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAC207.0290465E-445.00000426
TTGTGCG207.0290465E-445.0000041
TAGCGTA207.0290465E-445.0000041
TGGCGTG207.0290465E-445.00000423
GATTTCG207.0290465E-445.00000436
TATTGCG207.0290465E-445.0000041
CTCGGAA207.0290465E-445.00000418
AGACACG207.0290465E-445.00000424
TACTCGA406.8012014E-945.00000444
CGGAGTT207.0290465E-445.00000431
ACGACCA207.0290465E-445.00000428
ATTTCGA207.0290465E-445.00000437
AGTACGG600.044.9999962
TACGGGA1450.041.896554
TAGACGG700.041.7857172
ACGGGCT501.0786607E-940.55
TTACTCG451.924127E-840.043
CTACGGG403.452442E-739.3750043
CGTTCAA356.240449E-638.571439
GCCGATG356.240449E-638.571439