##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551140_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	471419
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.95663518016881	31.0	31.0	34.0	30.0	34.0
2	32.08858573795286	33.0	31.0	34.0	30.0	34.0
3	32.029941516994434	33.0	31.0	34.0	30.0	34.0
4	35.752124967385704	37.0	35.0	37.0	35.0	37.0
5	31.186937734796434	37.0	35.0	37.0	0.0	37.0
6	33.411892605092284	37.0	35.0	37.0	17.0	37.0
7	29.48575895328784	35.0	32.0	37.0	0.0	37.0
8	32.556848578440835	35.0	33.0	37.0	17.0	37.0
9	36.420144287777966	39.0	35.0	39.0	32.0	39.0
10	36.81431592702034	38.0	35.0	39.0	33.0	39.0
11	36.9730536953326	39.0	37.0	39.0	33.0	39.0
12	36.96626143621704	39.0	37.0	39.0	33.0	39.0
13	36.77008139256161	39.0	35.0	39.0	33.0	39.0
14	38.045783050746785	40.0	37.0	41.0	33.0	41.0
15	38.15196035798303	40.0	37.0	41.0	33.0	41.0
16	38.2058572098282	40.0	37.0	41.0	34.0	41.0
17	38.15112882594889	40.0	37.0	41.0	33.0	41.0
18	38.0664483187992	40.0	37.0	41.0	33.0	41.0
19	38.01157356831184	40.0	37.0	41.0	33.0	41.0
20	37.940420305503174	40.0	36.0	41.0	33.0	41.0
21	37.85225881858813	40.0	36.0	41.0	33.0	41.0
22	37.7997004787673	40.0	36.0	41.0	33.0	41.0
23	37.75648202554415	40.0	36.0	41.0	33.0	41.0
24	37.72684384804176	40.0	36.0	41.0	33.0	41.0
25	37.59740697765682	40.0	36.0	41.0	33.0	41.0
26	37.42676472522321	39.0	36.0	41.0	32.0	41.0
27	37.373864863316925	39.0	36.0	41.0	32.0	41.0
28	37.1643845496257	39.0	35.0	41.0	32.0	41.0
29	37.0957534592369	39.0	35.0	41.0	31.0	41.0
30	36.98659578845995	39.0	35.0	41.0	31.0	41.0
31	36.907638427810504	39.0	35.0	41.0	31.0	41.0
32	36.842590137436126	39.0	35.0	41.0	31.0	41.0
33	36.70202728358424	39.0	35.0	41.0	30.0	41.0
34	36.56905428079904	39.0	35.0	41.0	30.0	41.0
35	36.48196614900969	39.0	35.0	41.0	30.0	41.0
36	36.35085985079091	39.0	35.0	41.0	30.0	41.0
37	36.27400465403389	39.0	35.0	40.0	30.0	41.0
38	36.15655287546747	39.0	35.0	40.0	29.0	41.0
39	36.1454332557661	39.0	35.0	40.0	29.0	41.0
40	35.9817317503113	39.0	35.0	40.0	29.0	41.0
41	35.84845116552366	38.0	35.0	40.0	28.0	41.0
42	35.807298814854725	38.0	35.0	40.0	28.0	41.0
43	35.622717794573404	38.0	35.0	40.0	27.0	41.0
44	35.47112653499329	38.0	34.0	40.0	27.0	41.0
45	35.373928500972596	38.0	34.0	40.0	26.0	41.0
46	35.24020033134006	38.0	34.0	40.0	26.0	41.0
47	35.10136205795693	38.0	34.0	40.0	25.0	41.0
48	34.975476168758576	38.0	34.0	40.0	24.0	41.0
49	34.76019210086993	38.0	34.0	40.0	24.0	41.0
50	34.60451530379556	37.0	34.0	40.0	24.0	41.0
51	32.531868677333755	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	9.0
9	24.0
10	23.0
11	20.0
12	13.0
13	14.0
14	21.0
15	31.0
16	78.0
17	123.0
18	254.0
19	453.0
20	867.0
21	1366.0
22	1865.0
23	2397.0
24	3168.0
25	3840.0
26	4734.0
27	5689.0
28	6581.0
29	7967.0
30	9657.0
31	12769.0
32	16275.0
33	23329.0
34	33749.0
35	40638.0
36	46703.0
37	72168.0
38	90066.0
39	86513.0
40	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.0918545921993	20.95863764506734	25.59888337126845	11.350624391464919
2	31.473275366499863	25.161056300233973	25.524003063092493	17.841665270173667
3	32.05068102897847	25.18629923698451	25.99810359786093	16.764916136176097
4	28.785645041884184	26.6066917116196	25.685854834022386	18.92180841247383
5	22.715461192696942	39.76865590907452	20.344534267816954	17.171348630411586
6	25.683097202276535	35.672087887844995	24.170854377952523	14.47396053192595
7	69.44310687520019	20.00280005684964	6.235641753938641	4.318451314011527
8	83.11629357323315	6.050031924890596	6.946474367812923	3.887200134063328
9	76.63140433457285	8.615477950612936	8.923696329592145	5.829421385222064
10	40.887618021335584	29.22050235565389	15.883534605096528	14.008345017914001
11	31.447396053192595	27.02882149425458	23.673632161622677	17.85015029093015
12	30.469709536526953	23.32765544027712	27.251765414631144	18.95086960856478
13	25.176117212076733	25.357696656265443	27.530922597519403	21.93526353413842
14	19.610367846862346	29.114015345160038	27.94287035524661	23.33274645273101
15	19.203723227107943	27.38498024050791	32.57972207314512	20.83157445923902
16	22.590943513095567	26.383959916761945	30.701350603178913	20.32374596696357
17	22.601761914560083	25.618398919008357	27.566771810215542	24.21306735621602
18	23.466597655164513	27.62340932376506	27.161612069093522	21.748380951976902
19	25.472668687515775	27.722684066615898	25.688400340249334	21.116246905618993
20	27.040912648832567	26.9412136549439	26.493416684520565	19.524457011702964
21	26.35108046133058	27.33619137115814	25.766250405690055	20.546477761821226
22	23.75169435258231	26.592691427371406	26.27895778490048	23.376656435145804
23	23.756785365036198	27.60792416088448	26.137045812748323	22.498244661331
24	23.02155831648703	25.389727609621165	28.01626578479018	23.572448289101626
25	22.118964233516255	27.34022175601747	26.33771655363912	24.203097456827155
26	22.199571930702835	29.165561846255667	26.01910402423322	22.61576219880828
27	23.26953304809522	28.346545217736242	26.24565407843129	22.138267655737252
28	21.664591372006644	28.903798955918198	27.505255409731046	21.926354262344113
29	21.936960538289718	26.851272434925193	28.680218658984895	22.531548367800195
30	22.9012831472639	27.209128185329824	27.76723042558743	22.122358241818848
31	23.581569686414845	28.42609228732826	25.36003003697348	22.63230798928342
32	24.441314414565387	28.108540385516918	25.787250832062348	21.662894367855348
33	24.376404005778298	28.55060996692963	24.97056758425095	22.102418443041117
34	22.108357957570654	27.67538007589851	26.803968444207804	23.41229352232303
35	23.333170703768836	26.34153481297954	26.828575004401607	23.496719478850025
36	23.289472846872954	29.011982970563345	26.283200295278725	21.415343887284983
37	23.085832348717382	29.945547379295277	25.928526427657772	21.040093844329565
38	22.498668912368828	28.43160755081997	25.63006582254852	23.439657714262683
39	23.946849829981396	27.16182419461244	25.757977510452484	23.13334846495368
40	23.97378977088323	25.884828570761893	28.23708844997762	21.90429320837726
41	20.920030800625348	27.011639327222703	28.456001985494854	23.61232788665709
42	23.761452126452266	26.4060209707288	26.948213797068	22.884313105750934
43	23.81978664415308	26.309715985142727	27.160339315980053	22.710158054724143
44	22.76170455581977	27.347646149179393	26.37420214289199	23.516447152108846
45	21.56298324844777	27.159915064942226	26.040528701643336	25.236572984966664
46	21.70044058470278	28.993952301455817	25.9800729287534	23.325534185088
47	22.16181358833649	27.44734514306806	27.077186112566526	23.313655156028926
48	22.16138933729867	25.179511220379325	28.468729516629583	24.19036992569243
49	21.236309949323214	26.766634352879286	27.8758386912704	24.1212170065271
50	20.515507436060066	25.82182729164501	28.396818965718396	25.265846306576528
51	20.809513405272167	25.79361459762971	27.250916912555496	26.145955084542628
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	227.0
1	556.0
2	885.0
3	1389.0
4	1893.0
5	1492.5
6	1092.0
7	915.5
8	739.0
9	757.0
10	775.0
11	821.5
12	868.0
13	902.0
14	936.0
15	999.5
16	1063.0
17	1020.0
18	977.0
19	1081.0
20	1185.0
21	1250.0
22	1315.0
23	1674.5
24	2034.0
25	2734.5
26	4434.0
27	5433.0
28	6091.5
29	6750.0
30	8218.5
31	9687.0
32	11183.0
33	12679.0
34	14031.0
35	15383.0
36	15989.0
37	16595.0
38	17876.0
39	19157.0
40	22043.5
41	24930.0
42	26741.5
43	28553.0
44	30933.5
45	33314.0
46	35836.0
47	38358.0
48	37081.0
49	35804.0
50	34316.5
51	32829.0
52	29943.0
53	27057.0
54	25543.5
55	24030.0
56	23450.0
57	22870.0
58	23107.5
59	23345.0
60	22606.5
61	21868.0
62	19826.0
63	17784.0
64	14811.5
65	11839.0
66	10029.5
67	8220.0
68	7207.0
69	6194.0
70	5156.0
71	4118.0
72	3375.5
73	2633.0
74	2305.0
75	1577.0
76	1177.0
77	878.0
78	579.0
79	480.0
80	381.0
81	283.5
82	186.0
83	152.0
84	118.0
85	83.0
86	48.0
87	44.5
88	41.0
89	32.0
90	23.0
91	19.0
92	15.0
93	16.0
94	17.0
95	9.5
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	471419.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.02502778856641
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.71271538579771	25.467648769696854
2	8.432530828668172	5.906992532040913
3	4.063953386514188	4.270202408822941
4	2.663851908553239	3.7320594848681146
5	1.8978698867393495	3.3236472761064553
6	1.3955841154358126	2.932822345465272
7	1.0569474597028858	2.5913729903003784
8	0.8285680670903701	2.3216495659647176
9	0.6709908835087817	2.1151326906692827
>10	6.1654989370441085	43.293195983209955
>50	0.08497280472052365	1.920793695329063
>100	0.02410576020440387	1.6436506050030122
>500	0.0024105760204403874	0.4808316525230335
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	637	0.13512395554697626	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	626	0.1327905748389437	No Hit
GAGGAGCTGAGAACCCCAGTCTAATAATGTCCATCGACACCTCCTTATCCC	513	0.10882039120188199	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	502	0.10648701049384941	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06321340463579109	0.0
2	0.0	0.0	0.0	0.2948544712877504	0.0
3	0.0	0.0	0.0	0.41746302121891565	0.0
4	0.0	0.0	0.0	0.6015879716345756	0.0
5	0.0	0.0	0.0	1.1671146050541026	0.0
6	0.0	0.0	0.0	1.553819426030771	0.0
7	0.0	0.0	0.0	1.9125236785110487	0.0
8	0.0	0.0	0.0	2.555476126333474	0.0
9	0.0	0.0	0.0	2.8499063465834005	0.0
10	0.0	0.0	0.0	3.5509811865877277	0.0
11	0.0	0.0	0.0	4.292784126223169	0.0
12	0.0	0.0	0.0	4.96628264876893	0.0
13	0.0	0.0	0.0	5.307380483179507	0.0
14	0.0	0.0	0.0	5.468383752033754	0.0
15	0.0	0.0	0.0	5.67796376471886	0.0
16	0.0	0.0	0.0	6.078880995462635	0.0
17	0.0	0.0	0.0	6.541102501171993	0.0
18	0.0	0.0	0.0	7.088598465483996	0.0
19	0.0	0.0	0.0	7.38175593262045	0.0
20	0.0	0.0	0.0	7.698247206837229	0.0
21	0.0	0.0	0.0	8.058860588987717	0.0
22	0.0	0.0	0.0	8.461899074920613	0.0
23	0.0	0.0	0.0	8.860270799437442	0.0
24	0.0	0.0	0.0	9.185247094410705	0.0
25	0.0	0.0	0.0	9.480950067774103	0.0
26	0.0	0.0	0.0	9.734227937355092	0.0
27	0.0	0.0	0.0	10.004263722930132	0.0
28	0.0	0.0	0.0	10.283633031337303	0.0
29	0.0	0.0	0.0	10.570850983944219	0.0
30	0.0	0.0	0.0	10.9278582322732	0.0
31	0.0	0.0	0.0	11.228015841533752	0.0
32	0.0	0.0	0.0	11.540264605372291	0.0
33	0.0	0.0	0.0	11.837240331849161	0.0
34	0.0	0.0	0.0	12.15564073573615	0.0
35	0.0	0.0	0.0	12.478920026558114	0.0
36	0.0	0.0	0.0	12.814078346439155	0.0
37	2.1212551891205064E-4	0.0	0.0	13.15390342773626	0.0
38	2.1212551891205064E-4	0.0	0.0	13.482485856531026	0.0
39	2.1212551891205064E-4	0.0	0.0	13.825917071649636	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGAC	20	7.0290465E-4	45.000004	26
TTGTGCG	20	7.0290465E-4	45.000004	1
TAGCGTA	20	7.0290465E-4	45.000004	1
TGGCGTG	20	7.0290465E-4	45.000004	23
GATTTCG	20	7.0290465E-4	45.000004	36
TATTGCG	20	7.0290465E-4	45.000004	1
CTCGGAA	20	7.0290465E-4	45.000004	18
AGACACG	20	7.0290465E-4	45.000004	24
TACTCGA	40	6.8012014E-9	45.000004	44
CGGAGTT	20	7.0290465E-4	45.000004	31
ACGACCA	20	7.0290465E-4	45.000004	28
ATTTCGA	20	7.0290465E-4	45.000004	37
AGTACGG	60	0.0	44.999996	2
TACGGGA	145	0.0	41.89655	4
TAGACGG	70	0.0	41.785717	2
ACGGGCT	50	1.0786607E-9	40.5	5
TTACTCG	45	1.924127E-8	40.0	43
CTACGGG	40	3.452442E-7	39.375004	3
CGTTCAA	35	6.240449E-6	38.57143	9
GCCGATG	35	6.240449E-6	38.57143	9
>>END_MODULE