##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551136_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	664560
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.818206031058143	31.0	31.0	34.0	30.0	34.0
2	31.940890213073313	33.0	31.0	34.0	30.0	34.0
3	31.68278259299386	33.0	31.0	34.0	30.0	34.0
4	35.51786144215722	37.0	35.0	37.0	33.0	37.0
5	30.97812838569881	37.0	35.0	37.0	0.0	37.0
6	33.28942157216805	37.0	35.0	37.0	17.0	37.0
7	29.697100337065127	35.0	32.0	37.0	0.0	37.0
8	32.721853557240884	35.0	35.0	37.0	17.0	37.0
9	36.55225713253882	39.0	35.0	39.0	32.0	39.0
10	36.84221590225111	38.0	37.0	39.0	33.0	39.0
11	36.95028590345492	39.0	37.0	39.0	33.0	39.0
12	36.99081046105694	39.0	37.0	39.0	33.0	39.0
13	36.81763271939328	39.0	37.0	39.0	33.0	39.0
14	37.977716082821715	40.0	37.0	41.0	33.0	41.0
15	38.089496809919346	40.0	37.0	41.0	33.0	41.0
16	38.1561454195257	40.0	37.0	41.0	33.0	41.0
17	38.12592993860599	40.0	37.0	41.0	33.0	41.0
18	38.021585710846274	40.0	37.0	41.0	33.0	41.0
19	37.90902552064524	40.0	37.0	41.0	33.0	41.0
20	37.77716233297219	40.0	36.0	41.0	33.0	41.0
21	37.68475532683279	40.0	36.0	41.0	33.0	41.0
22	37.68559046587215	39.0	36.0	41.0	33.0	41.0
23	37.62996268207536	39.0	36.0	41.0	33.0	41.0
24	37.56989135668713	39.0	35.0	41.0	33.0	41.0
25	37.439891055736126	39.0	35.0	41.0	33.0	41.0
26	37.30255055976887	39.0	35.0	41.0	32.0	41.0
27	37.252797339593116	39.0	35.0	41.0	32.0	41.0
28	37.12490068616829	39.0	35.0	41.0	32.0	41.0
29	37.086828879258455	39.0	35.0	41.0	32.0	41.0
30	36.937734741784034	39.0	35.0	41.0	31.0	41.0
31	36.810855302756714	39.0	35.0	41.0	31.0	41.0
32	36.69115204044782	39.0	35.0	41.0	31.0	41.0
33	36.445071927290236	39.0	35.0	40.0	30.0	41.0
34	36.211046406644996	39.0	35.0	40.0	29.0	41.0
35	36.03884224148309	39.0	35.0	40.0	29.0	41.0
36	35.85957927049476	39.0	35.0	40.0	27.0	41.0
37	35.724670458649335	39.0	35.0	40.0	27.0	41.0
38	35.552646864090526	39.0	35.0	40.0	26.0	41.0
39	35.494962080173345	39.0	35.0	40.0	25.0	41.0
40	35.372151498736	38.0	35.0	40.0	25.0	41.0
41	35.274852534007465	38.0	34.0	40.0	24.0	41.0
42	35.24570091489105	38.0	34.0	40.0	24.0	41.0
43	35.10139942217407	38.0	34.0	40.0	24.0	41.0
44	34.961211929697846	38.0	34.0	40.0	24.0	41.0
45	34.834820031298904	38.0	34.0	40.0	23.0	41.0
46	34.75852744673167	38.0	34.0	40.0	23.0	41.0
47	34.62874383050439	38.0	34.0	40.0	23.0	41.0
48	34.54202178885277	38.0	34.0	40.0	23.0	41.0
49	34.40438034188034	38.0	33.0	40.0	23.0	41.0
50	34.230778259299385	37.0	33.0	40.0	22.0	41.0
51	32.25677440712652	35.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	16.0
9	30.0
10	23.0
11	23.0
12	28.0
13	20.0
14	22.0
15	46.0
16	81.0
17	184.0
18	315.0
19	612.0
20	1037.0
21	1674.0
22	2606.0
23	3749.0
24	5593.0
25	7946.0
26	10194.0
27	11108.0
28	11901.0
29	12846.0
30	15124.0
31	18189.0
32	23615.0
33	32007.0
34	44561.0
35	53272.0
36	65030.0
37	99995.0
38	124556.0
39	118141.0
40	15.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.76661249548574	20.764566028650535	25.875315998555436	13.593505477308293
2	33.41112916817142	24.06464427591188	26.017816299506443	16.506410256410255
3	31.7515348501264	23.29541350668111	28.704405922715782	16.248645720476706
4	26.409353557240884	25.843866618514504	29.473185265438783	18.273594558805826
5	21.088238834717707	39.238744432406406	23.87308896111713	15.799927771758757
6	24.857349223546407	34.21767786204406	27.116287468400145	13.808685446009388
7	69.30043938846757	18.506831587817505	8.477940291320573	3.714788732394366
8	83.72788010111954	4.207295052365475	8.905591669676177	3.1592331768388107
9	77.72390754785121	7.037137354038762	10.513723365836041	4.725231732273985
10	39.16681714216925	30.96138798603587	17.599765258215964	12.27202961357891
11	28.468911761165284	26.211929697845193	27.86369928975563	17.455459251233897
12	26.755146262188518	23.616528229204288	31.292434091729866	18.33589141687733
13	23.79138076321175	24.103918382087397	33.12642951727459	18.978271337426268
14	20.305916696761766	26.81909835078849	32.52633321295293	20.34865173949681
15	19.28614421572168	26.460966654628628	35.5206452389551	18.732243890694594
16	24.117160226315157	25.840857108462746	32.222523173227394	17.819459491994703
17	24.09714698447093	25.904357770554952	29.684151920067414	20.314343324906705
18	24.727789815817985	25.84175996147827	30.430961839412547	18.9994883832912
19	24.052154809197063	27.69005055976887	29.161099073070908	19.096695557963166
20	25.25084266281449	27.498194293968943	29.143643914770674	18.10731912844589
21	24.41735885397857	27.08604189237992	30.548182255928737	17.948416997712773
22	23.5641627543036	24.53232213795594	30.784278319489587	21.119236788250873
23	21.958589141687735	26.826622125917897	31.11607680269652	20.098711929697846
24	21.549596725653064	25.9431804502227	31.621524015890213	20.885698808234018
25	22.367280606717227	26.993348982785605	29.710334657517755	20.929035752979416
26	21.232996268207536	28.00093294811605	28.862405200433372	21.903665583243047
27	21.476014204887445	27.251715420729504	30.333905140243168	20.938365234139884
28	20.20825809558204	27.619477549055016	30.817683881064163	21.354580474298785
29	21.628144938004095	26.589623209341518	30.065908270133622	21.716323582520765
30	22.239827856025038	26.114872998675814	30.258366437943902	21.386932707355243
31	22.696069579872397	26.375947995666305	29.241904417960757	21.68607800650054
32	23.856085229324666	26.194625015047553	29.026122547249305	20.923167208378477
33	22.805465270254004	27.28451908029373	28.473426026242926	21.436589623209343
34	21.094408330323823	27.160828217166245	30.30892620681353	21.4358372456964
35	21.390845070422536	27.15210063801613	29.248976766582402	22.208077524978933
36	21.43267726014205	28.24620801733478	29.01634163958108	21.3047730829421
37	21.56720235945588	28.4037558685446	29.21481882749488	20.814222944504635
38	21.934663536776213	27.286776212832553	28.93929818225593	21.839262068135305
39	22.44537739256049	25.97237269772481	28.904989767665825	22.677260142048876
40	22.275641025641026	25.95476706392199	29.989767665824	21.77982424461298
41	19.894516672685686	26.919615986517393	29.488834717707956	23.697032623088962
42	21.2080173347779	26.751233899121225	29.54496208017335	22.49578668592753
43	21.512880703021548	26.22366678704707	29.601089442638738	22.662363067292645
44	21.777416636571566	26.40062597809077	28.957806669074277	22.864150716263392
45	21.57878897315517	26.789304201276032	28.091368725171538	23.540538100397253
46	21.07003129890454	27.100487540628386	28.389912122306487	23.439569038160588
47	20.85906464427591	27.009901288070303	29.573251474659923	22.55778259299386
48	20.89547971590225	26.48143132298062	29.718911761165284	22.904177199951846
49	21.349313831708198	25.64192849404117	29.90760804141086	23.101149632839775
50	19.828909353557243	26.503250270855904	29.697544239797764	23.97029613578909
51	19.910316600457445	26.571265198025763	28.446942337787405	25.071475863729386
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	244.0
1	534.0
2	824.0
3	4743.0
4	8662.0
5	6748.0
6	4834.0
7	3895.0
8	2956.0
9	2771.0
10	2586.0
11	2535.0
12	2484.0
13	2479.5
14	2475.0
15	2376.0
16	2277.0
17	2296.0
18	2315.0
19	2416.0
20	2517.0
21	2699.5
22	2882.0
23	3337.5
24	3793.0
25	4621.5
26	6187.0
27	6924.0
28	8677.0
29	10430.0
30	12247.5
31	14065.0
32	16368.5
33	18672.0
34	20511.0
35	22350.0
36	24768.0
37	27186.0
38	28954.0
39	30722.0
40	32679.0
41	34636.0
42	37382.0
43	40128.0
44	42300.5
45	44473.0
46	48135.0
47	51797.0
48	52788.0
49	53779.0
50	52839.5
51	51900.0
52	47038.0
53	42176.0
54	38945.0
55	35714.0
56	33303.0
57	30892.0
58	29209.5
59	27527.0
60	25253.5
61	22980.0
62	20447.5
63	17915.0
64	14980.5
65	12046.0
66	10310.0
67	8574.0
68	7050.0
69	5526.0
70	4702.5
71	3879.0
72	3207.0
73	2535.0
74	2095.5
75	1226.0
76	796.0
77	619.5
78	443.0
79	336.5
80	230.0
81	177.0
82	124.0
83	107.5
84	91.0
85	61.5
86	32.0
87	28.5
88	25.0
89	20.0
90	15.0
91	12.0
92	9.0
93	9.0
94	9.0
95	6.5
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	664560.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.50732295487859
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.252796265976	31.23542117869548
2	8.589770055254217	7.130767195831641
3	3.790892587792967	4.720494087863343
4	2.338233575951742	3.882152647238784
5	1.683020036163092	3.4928828090276447
6	1.2316139467703777	3.0672598706598424
7	0.9471549323884918	2.751970596686873
8	0.7540324024083019	2.5038293156163487
9	0.5928514051989958	2.2146907265863476
>10	4.7688579879664506	36.154329347982916
>50	0.039576038733198156	1.0844889149791626
>100	0.009333971163411591	0.6673062550870227
>500	0.0011200765396093904	0.28809157645303646
>1k	7.467176930729269E-4	0.8063154772916346
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3970	0.5973877452750692	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1233	0.1855362946912243	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	775	0.11661851450583845	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.514265077645359E-4	0.0	0.0	0.06635969664138679	0.0
2	4.514265077645359E-4	0.0	0.0	0.2163837727218009	0.0
3	4.514265077645359E-4	0.0	0.0	0.3302937281810521	0.0
4	4.514265077645359E-4	0.0	0.0	0.4873901528831106	0.0
5	4.514265077645359E-4	0.0	0.0	0.9607860840255207	0.0
6	4.514265077645359E-4	0.0	0.0	1.431022029613579	0.0
7	4.514265077645359E-4	0.0	0.0	1.8407668231611893	0.0
8	4.514265077645359E-4	0.0	0.0	2.577193932827736	0.0
9	4.514265077645359E-4	0.0	0.0	2.974298784157939	0.0
10	6.019020103527145E-4	0.0	0.0	3.706061153244252	0.0
11	6.019020103527145E-4	0.0	0.0	4.4086312748284575	0.0
12	6.019020103527145E-4	0.0	0.0	5.0218189478752855	0.0
13	6.019020103527145E-4	0.0	0.0	5.324575659082702	0.0
14	7.523775129408932E-4	0.0	0.0	5.484079691826171	0.0
15	7.523775129408932E-4	0.0	0.0	5.672324545563982	0.0
16	7.523775129408932E-4	0.0	0.0	6.003671602263152	0.0
17	7.523775129408932E-4	0.0	0.0	6.43177440712652	0.0
18	7.523775129408932E-4	0.0	0.0	6.907427470807752	0.0
19	7.523775129408932E-4	0.0	0.0	7.2050680149271695	0.0
20	7.523775129408932E-4	0.0	0.0	7.520163717346816	0.0
21	7.523775129408932E-4	0.0	0.0	7.904478150957024	0.0
22	7.523775129408932E-4	0.0	0.0	8.322499097146984	0.0
23	7.523775129408932E-4	0.0	0.0	8.736607680269652	0.0
24	7.523775129408932E-4	0.0	0.0	9.07141567352835	0.0
25	7.523775129408932E-4	0.0	0.0	9.38440471891176	0.0
26	7.523775129408932E-4	0.0	0.0	9.671511977850006	0.0
27	7.523775129408932E-4	0.0	0.0	9.986306729264475	0.0
28	7.523775129408932E-4	0.0	0.0	10.317653785963644	0.0
29	7.523775129408932E-4	0.0	0.0	10.664349343926808	0.0
30	7.523775129408932E-4	0.0	0.0	11.051823763091368	0.0
31	7.523775129408932E-4	0.0	0.0	11.426507764535934	0.0
32	7.523775129408932E-4	0.0	0.0	11.78794992175274	0.0
33	7.523775129408932E-4	0.0	0.0	12.145178764897075	0.0
34	7.523775129408932E-4	0.0	0.0	12.50827615264235	0.0
35	7.523775129408932E-4	0.0	0.0	12.887775370169736	0.0
36	9.028530155290719E-4	0.0	0.0	13.262308896111714	0.0
37	9.028530155290719E-4	0.0	0.0	13.660166124954857	0.0
38	9.028530155290719E-4	0.0	0.0	14.08872035632599	0.0
39	9.028530155290719E-4	0.0	0.0	14.728090766823161	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTAAG	20	7.0311356E-4	45.000004	2
TACTCGA	20	7.0311356E-4	45.000004	44
GTTAACG	25	3.889024E-5	45.0	1
TGCTACG	25	3.889024E-5	45.0	1
ACGTAAG	35	1.2109012E-7	45.0	1
AATAGCG	35	1.2109012E-7	45.0	1
CGTTATT	375	0.0	42.6	1
CGTTTTT	1800	0.0	42.000004	1
ATTACGG	50	1.0804797E-9	40.5	2
TCTCGTA	50	1.0804797E-9	40.5	28
TACGGTT	45	1.9261279E-8	40.000004	33
TCGTACG	45	1.9261279E-8	40.000004	30
CGACGGT	40	3.4552795E-7	39.375004	28
ACGCACG	40	3.4552795E-7	39.375004	1
TTAACGG	75	0.0	39.0	2
TAACGTG	35	6.2443123E-6	38.571426	44
CTAACGT	35	6.2443123E-6	38.571426	43
TTACGCG	35	6.2443123E-6	38.571426	1
CGGTTTT	570	0.0	38.289474	1
TAGACGG	90	0.0	37.500004	2
>>END_MODULE